목재 부후균의 목질소 분해효소 수도에 관하여

홍순우,김규중,신광수 한국균학회 1985 韓國菌學會誌 Vol.14 No.1

Among the representative phenolic compounds in relation to lignin derivatives and protein synthesis inhibitors, the most effective inducer for the extracellular polyphenol oxidase (PO) of Lentinus edodes JA01 was gallic acid and ferulic acid for Pleurotus ostreatus. Optimum concentration of these inducers was 2.0 mM and 1.0 mM, respectively. Addition of gallic acid after two days culture had the best effect on production of PO enzyme of L. edodes JA01 and for P. ostreatus, and addition of ferulic acid after three days culture had the best effect. Also, in case of L. edodes JA01, polyphenol oxidase activity was parallel to growth curve, whereas the maximum enzyme activity of P. ostreatus was shown at exponential growth phase and declined thereafter.

각종 폐기물의 자원화

홍순우,Hong, Sun-U 환경보전협회 1985 환경정보 Vol.7 No.21

한강에서 분리한 이종 coliphage의 동정과 점토질에 대한 흡착 및 용출효과

홍순우,하영칠,안태석,이영숙 한국미생물학회 1982 미생물학회지 Vol.20 No.4

Coliphages isolated from Han-River from September 1980 to August 1981 were classified by morphological and physiological characteristics. Effects of soil metrial on the fate of coliphage in nature were investigated. 1. The correlation coefficient between coliphage and E.coli which was host of coliphages in nature was 0.7173 (p=0.004). 2. Coliphage I isolated from Han-River of which DNA molecular weight was $27{\times}10^6$ daltons was identified as $T_1$ phage and coliphage II of which DNA molecular weight $72{\times}10^6$ daltons was classified as $T_5$ phage. 3. Soil material SW was composed of 63.65% silt and 21.92% clay. Clay was consisted of illite, kaolinite and chlorite evenly. Soil material J was composed of 68.92% silt and 11.67% clay. Clay consisted of smectite only. 4. Coliphage was absorbed to soil material J more than soil material SW, and $T_1$ coliphage was absorbed to soil material more than $T_5$ coliphage was. 5. The phage adsorption efficiency to soil material was enhanced at lower pH : the phage adsorption efficiency at pH 4 was 27 time higher than at pH 7. 6. Divalent $(Ca^{2+})\;and\;trivalention\;(Al^{3+})$ enhanced the phage adsorption efficiency to soil material from 4 to 39 and from 17 to 91 times higher than monovalent $ion(Na^+)$, respectively. 7. The concentration of organic compound was inversely related to the phage adsorption efficiency to soil. 8. Adsorption of phage onto soil material, and elution efficiency of elutants was in the order of D.D.W>tap water>river water>seawater. 9. The higher the concentration of organic compound was, the more were adsorbed phages to soil eluted. 10. Coliphages survived longer in sterile soil suspension than in nonsterile soil material suspension.

Trichoderma koningii의 種間 原形質體 融合에 대한 硏究

홍순우,하영칠,박희문,조남진,Hong, Soon-Woo,Hah, Yung-Chil,Park, Hee-Moon,Cho, Nam-Jin 한국미생물학회 1984 미생물학회지 Vol.22 No.2

The conditions for the protoplast fusion of auxotrophic mutants of Trichoderma koningii were determined. A preparation of commercial enzyme Driselase was used successfully to isolate protoplasts from the 18 hr old mycelium of T. koningii. The yields of protoplasts production were ranged from $0.3{\times}10^8$ to $2.5{\times}10^8$ protoplasts per mg of damp mycelium of various auxotrophic mutant strains. The regeneration frequencies from $9.3{\times}10^{-3}\;to\;2.0{\times}10^{-1}$ were obtained when the protoplasts from auxotrophic mutants were plated on the malt extract medium containing 0.6M $MgSO_4$, and 2% agar, and the optimal concentration of PEG for protoplst fusion was 30%. Exposure of protoplasts to PEG for 10 min was found to be sufficient to induce high frequency heterokaryon formation. Optimal pH of fusion mixture was determined as 5.5, and 1 mM of calcium chloride in fusion mixture was found to be sufficient to enhance protoplast fusion frequency. Under optimal condition, the fusion frequency of the cross between protoplasts from various auxotrophic mutants were $1.6{\times}10^{-2}\;and\;4.1{\times}10^{-2}$.

연안지의 개발과 환경관리

홍순우,Hong, Sun-U 환경보전협회 1985 환경정보 Vol.7 No.3

Salicylate를 생분해하는 pseudomonas putida의 특성

홍순우,하영칠,이계준,배경숙 한국미생물학회 1986 미생물학회지 Vol.24 No.1

Eight strains of the bacteria capable of growing on salicylate as the sole carbon source were isolated form soil and river water. Three of these isolates were identified as Acinetobacter calcoaceticus (AcBl), Pseuomonas putida biotype B (PpB2), and P. putida biotype A (PpB3). Effects of temperature, pH and C source concentration on biodegradation of salicylate by PpB3 were wxamined. The optimum conditions were as follows; $30^{\circ}C$ for temperature, 7.0 for pH, and 10mM for C source concentration. Ultraviolet scanning spectrum of the salicylate was measured. The spectrum has two peaks at 225nm and 292nm. The spectra of the culture filtrates indicate that ring degradation of salicylate is accomplished.

Rhizosphere와 해수에 있어서의 Azotobacter의 분포

홍순우,최영길 한국미생물학회 1974 미생물학회지 Vol.12 No.1

This experiment has been carried out with a view to elucidating the distribution of Azotobacter and their population size in rhizosphere and sea and designed ro compare the results with some environmental factors. Results of the experiment are summarized as follows: 1) It was observed that the population sizes of Azotobacter were decided upon the moisture content of soil and that the soil pH was one of the most impertant factors influencing the distribution of Azotobacter. 2)Population sizes of Azotobacter in rhizosphere were changed in accordance with the kinds of vegetation on soil: The rhizosphere where bamboo, corn, legume, and oak inhabit showed the largest population size of Azotobacter. On the other hand, rhizosphere of ginseng revealed no Azotobacter. However, the largest population of general fungi were measured at the rhizosphere. 3)Comparing the population sizes of general microbes in rhizosphere with those of non-rhizosphere, the population sizes of microbes in rhizosphere are larger than those of non-rhizosphere. 4)In coastal environments, population sizes of Azotobacter in surface water of sea are similar to those of the soil(mud) of tidal land. But the sizes are generally smaller than those of terrestrial soils.

효모의 미토콘드리아와 M-DNA

홍순우 한국미생물학회 1971 미생물학회지 Vol.9 No.1

saccharomyces cerevisiae J170, mutant를 사용하여 YEP 배지에서 20시간 진탕 배양하여 생장이 왕성한 영양시기의 효모세포를 얻었다. 이 세포를 sporulation medium에 접종하여 3$0^{\circ}C$에서 20시간 진탕 배양하면 약 80%의 ascospore를 형성함을 볼 수 있었다. 포자형성시기와 생장시기를 택하여 D-C-glucose 운반에 관하여 비교 검토한 결과는 다음과 같다. haploid인 포자형성시나 diploid인 생장시기의 포도당 흡수는 PH6.0에서 최대이었다. 이 두 시기는 포도당 운반은 2,4-dinitrophenol에 의하여 저해되었으므로 포자형성기의 포도당운반도 역시 energy를 요구하는 능동수송과 연관이 있음을 알게 되었아. 생장시기의 포도당 흡수정도는 포자형성기 보다 높았으며 이때에 fructose나 galactose와 포도당흡수의 관계는 서로 경쟁적으로 작용함을 알 수 있었다. 효모세포의 당 성분은 ribose, mannose, $\alpha$,$eta$-glucose로 되어 있으며 특히 포자형성기의 mannose 함량은 영양세포 때보다 현저히 적었다. During the sporulation stage in Saccharomyces cerevisiae J170, the incorporation of D$^{14}$ C-glucose into starved cells of sporulation stage as well as the vegetative one is appeared higher at pH 6.0. Glucose transport system, in both the vegetative and sporulation stage, is associated with "energy dependent" as the result of repression by such a respiratory inhibitor as 2,4-dinitrophenol. The Km value of glucose uptake in vegetative stage and sporulation stage was 2.1 mM and 2.5 mM respectively, indicating that the glucose is considerably reuqired for vegetative growth. Competition and countertranspoer of glucose by frutose and galactose are more distinct in vegetative stage, comparing with sporulation stage. The main sugar components of yeast cells consists of ribose, mannose, and .apha., .betha.-glucose. Amounts of mannose is lower in the aporulation stage than that in the vegetative stage.ive stage.

해안간척지 토양의 생물학적 토성개량에 관한 연구 (제 2 ) -간척지토양에 있어서 생물의 화에 대하여-

홍순우,하영칠,이광웅 한국미생물학회 1968 미생물학회지 Vol.6 No.4

The soil of the reclaimed tidal land, located in Chogi-ri, Is. Kanghwa, Korea was used in this experiment. The experimented soil samples were collected from 18 sites with its time elapsed after the shore-protection works, soil-depth and the vegetation of saline plants, and at each site samplings were conducted monthly from March through October, 1968, for the purposes of examining the changes of microbial populations for the microbes such as bacteria, actinomycetes and fungi, by using the dilution plate method. The numbers of the microbes in these soils generally showed lower levels comparing with those of other soils. The more time elapsed after the reclamation, the higher numbers of the microbes inhibited the soils. Higher populations were there in the surface soils than in the lower part of the area. The surface soils included comparatively better conditions in aeration and contents of organic matter than in the lower part, and this fact was. same as in general soils. However, not so was this in the case of March, April and October due to the higher soil temperatures in the lows. At the experimental sites where the halophytes such as Salicorniu were grown vigourously, the more densly the plants grew, the higher populations of actinomycetes and fungi were, but not in the case of bacterial population. This means, in this soil with dense Salicornia, it is difficult to obtain good-natured soils in short time without a higher population of bacteria. For the rapid utilization of the land soil, in this view of point, the methods increasing the number of bacteria in the soil are needed as well as the cultivation and harvesting Salicorniu which indicated in the privious paper(Hong, et al., 1969a). According to the results of this experiment, the changes of soil-microbial populations in the reclaimed tidal land soil containing high salinity depend deeply upon the interrelations of many environmental factors such as soil-salinity, soil-components and contents, concentration of organic matters, pH, aeration, and air and soil temperatures, as in the general soils.

人類平和와 環境科學

홍순우 세계평화교수협의회 1983 아카데미論叢 Vol.11 No.1

科學人의 立場에서 平和란 한글학회가 지은 「한글사전」의 定義대로 「平穩하여 잘 和合하는 것」이라는 生物學的 해석으로 받아들일 수 있다고 筆者는 생각한다. 즉 나날이 변모하는 地球의 環境條件에서 비록 最適狀態는 아니나 能動的으로 惡化一路에 있는 地球環境을 改善하고 알맞게 適應하는 것이 人間生存의 길이요 平和요 現象이며, 하나의 科學人으로서의 平和를 向한 具現策이라고 생각한다.