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        Genome-wide identification and functional prediction of silicon (Si) transporters in poplar (Populus trichocarpa)

        Hassan Md Mahmudul,Martin Samir,Feng Kai,Yates Timothy B.,Yuan Guoliang,Martin Madhavi Z.,Martin Stanton,Muchero Wellington,Griffiths Natalie A.,Weston David J.,Yang Xiaohan 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2

        Silicon (Si) enhances plant tolerance to various biotic and abiotic stressors such as salinity, drought, and heat. In addition, Si can be biomineralized within plants to form organic carbon-containing phytoliths that can have ecosystem-level consequences by contributing to long-term carbon sequestration. Si is taken up and transported in plants via different transporter proteins such as influx transporters (e.g., Lsi1, Lsi6) and efflux transporters (e.g., Lsi2). Additionally, the imported Si can be deposited in plant leaves via silicification process using the Siliplant 1 (e.g., Slp1) protein. Functional homologs of these proteins have been reported in different food crops. Here, we performed a genome-wide analysis to identify different Si transporters and Slp1 homologs in the bioenergy crop poplar (Populus trichocarpa Torr. and A. Gray ex W. Hook). We identified one channel-type Si influx transporter (PtLsi1; Potri.017G083300), one Si efflux transporter (PtLsi2; Potri.012G144000) and two proteins like Slp1 (PtSlp1a; Potri.004G168600 and PtSlp1b; Potri.009G129900) in the P. trichocarpa genome. We found a unique sequence (KPKPPVFKPPPVPI) in PtSlp1a which is repeated six times. Repeated presence of this sequence in PtSlp1a indicates that this protein might be important for silicification processes in P. trichocarpa. The mutation profiles of different Si transporters in a P. trichocarpa genome-wide association study population identified significant and impactful mutations in Potri.004G168600 and Potri.009G129900. Using a publically accessible database (http://bar.utoronto.ca/eplant_poplar/), digital expression analysis of the putative Si transporters in P. trichocarpa found low to moderate expression in the anticipated tissues, such as roots and leaves. Subcellular localization analysis found that PtLsi1/PtLsi2 are localized in the plasma membrane, whereas PtSlp1a/PtSlp1b are found in the extracellular spaces. Protein–Protein interaction analysis of PtLsi1/PtLsi2 identified Delta-1-pyrroline-5-carboxylate synthase (P5CS) as one of the main interacting partners of PtLsi2, which plays a key role in proline biosynthesis. Proline is a well-known participant in biotic and abiotic stress tolerance in plants. These findings will reinforce future efforts to modify Si accumulation for enhancing plant stress tolerance and carbon sequestration in poplar.

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        The <i>UV</i>–optical colour dependence of galaxy clustering in the local universe

        Loh, Yeong-Shang,Rich, R. Michael,Heinis, Sé,bastien,Scranton, Ryan,Mallery, Ryan P.,Salim, Samir,Martin, D. Christopher,Wyder, Ted,Arnouts, Sté,phane,Barlow, Tom A.,Forster, Karl,Friedman Blackwell Publishing Ltd 2010 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.407 No.1

        <P>ABSTRACT</P><P>We measure the UV-optical colour dependence of galaxy clustering in the local Universe. Using the clean separation of the red and blue sequences made possible by the <I>NUV</I>−<I>r</I> colour–magnitude diagram, we segregate the galaxies into red, blue and intermediate ‘green’ classes. We explore the clustering as a function of this segregation by removing the dependence on luminosity and by excluding edge-on galaxies as a means of a non-model dependent veto of highly extincted galaxies. We find that ξ(<I>r</I><SUB>p</SUB>, π) for both red and green galaxies shows strong redshift-space distortion on small scales – the ‘finger-of-God’ effect, with green galaxies having a lower amplitude than is seen for the red sequence, and the blue sequence showing almost no distortion. On large scales, ξ(<I>r</I><SUB>p</SUB>, π) for all three samples show the effect of large-scale streaming from coherent infall. On scales of 1 <I>h</I><SUP>−1</SUP> Mpc < <I>r</I><SUB>p</SUB> < 10 <I>h</I><SUP>−1</SUP> Mpc, the projected auto-correlation function <I>w</I><SUB>p</SUB>(<I>r</I><SUB>p</SUB>) for red and green galaxies fits a power law with slope γ∼ 1.93 and amplitude <I>r</I><SUB>0</SUB>∼ 7.5 and 5.3, compared with γ∼ 1.75 and <I>r</I><SUB>0</SUB>∼ 3.9 <I>h</I><SUP>−1</SUP> Mpc for blue sequence galaxies. Compared to the clustering of a fiducial <I>L</I>* galaxy, the red, green and blue have a relative bias of 1.5, 1.1 and 0.9, respectively. The <I>w</I><SUB>p</SUB>(<I>r</I><SUB>p</SUB>) for blue galaxies display an increase in convexity at ∼ 1 <I>h</I><SUP>−1</SUP> Mpc, with an excess of large-scale clustering. Our results suggest that the majority of blue galaxies are likely central galaxies in less massive haloes, while red and green galaxies have larger satellite fractions, and preferentially reside in virialized structures. If blue sequence galaxies migrate to the red sequence via processes like mergers or quenching that take them through the green valley, such a transformation may be accompanied by a change in environment in addition to any change in luminosity and colour.</P>

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