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Expression of ErbB4 in the apoptotic neurons of Alzheimer's disease brain
Ran-Sook Woo,Ji-Hye Lee,Ha-Nul Yu,Dae-Yong Song,Tai-Kyoung Baik 대한해부학회 2010 Anatomy & Cell Biology Vol.43 No.4
Neuregulin-1 (NRG1) signaling participates in the synaptic plasticity, maintenance or regulation of adult brain. Although ErbB4, a key NRG1 receptor, is expressed in multiple regions in the adult animal brain, little is known about its localization in Alzheimer's disease (AD) brains. We previously reported that ErbB4 immunoreactivity showed regional difference in the hippocampus of age-matched control. In the present paper, immunohistochemical characterization of the distribution of ErbB4 receptor in the hippocampus relative to pathology staging were performed in age-matched control (Braak stage 0, n=6) and AD (Braak stage I/V, n=10). Here, we found that ErbB4 immunoreactivity was significantly increased in apoptotic hippocampal pyramidal neurons in the brains of AD patients, compared to those of age-matched control subjects. In AD brains, ErbB4 immunoreactivity was demonstrated to colocalize with the apoptotic signal Bax in apoptotic hippocampal pyramidal neurons. These results suggest that up-regulation of ErbB4 immunoreactivity in apoptotic neuron may involve in the progression of pathology of AD.
Isolation and Characterization of a Type Ⅱ Peroxiredoxin Gene from Panax ginseng C. A. Meyer
Yu-Jin Kim,Jung-Hye Lee,Ok Ran Lee,Ju-Sun Shim,Seok-Kyu Jung,Na-Ri Son,Ju-Han Kim,Se-Young Kim,Deok-Chun Yang 고려인삼학회 2010 Journal of Ginseng Research Vol.34 No.4
A peroxiredoxin cDNA (PgPrx) was isolated and characterized from the leaves of Panax ginseng. The cDNA is 716 nucleotides long and has an open reading frame of 489 base pairs with a deduced amino acid sequence of 162 residues. The calculated molecular mass of the mature protein is approximately 17.4 kDa with a predicted isoelectric point of 5.37. A GenBank BlastX search revealed that the deduced amino acid sequence of PgPrx shares a high degree homology with type Ⅱ peroxiredoxin (Prx) proteins in other plants. The PgPrx gene was highly expressed in leaves, and expressed at a low level in the stem. To analyze the gene expression of PgPrx in response to various abiotic stresses, we utilized real-time quantitative RTPCR. Our results reveal that PgPrx expression is induced by ultraviolet irradiation, low temperature, and salt. The induction of PgPrx in response to abiotic stimuli suggests that ginseng Prx may function to protect the host against environmental stresses.
Ran-Sook Woo,Ji-Hye Lee,Ha-Nul Yu,Dae-Yong Song,Tai-Kyoung Baik 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.2
Neuregulin-1 (NRG1) plays important roles in the development and plasticity of the brain, and has also been reported to exhibit potent neuroprotective properties. Although ErbB4, a key NRG1 receptor, is expressed in multiple regions in the adult animal brain, little is known about its role in Alzheimer’s disease (AD). AD is characterized by progressive impairment of cognition and behavioral disturbance that strongly correlate with degeneration and death of neurons in the cerebral cortex and limbic brain areas, such as the hippocampus and the amygdala. Here, we show that the ErbB4 and phospho- ErbB4 immunoreactivities were higher intensity in the neurons of the CA1-2 transitional fi eld of AD brains as compared to age-matched controls. Also, ErbB4 expression was increased in the neurons of the cortico medial nucleus amygdala, human basal forebrain and superior frontal gyrus of AD brains. In cerebral cortex and hippocampus of amyloid precursor protein/presenilin 1 double transgenic mice, ErbB4 immunoreactivity significantly increased in comparison to age-matched wild type control. These results suggest that up-regulating of ErbB4 immunoreactivity may involve in the progression of pathology of AD.
Neuregulin 1 Controls Glutamate Uptake by Up-regulating Excitatory Amino Acid Carrier 1 (EAAC1)
Yu, Ha-Nul,Park, Woo-Kyu,Nam, Ki-Hoan,Song, Dae-Yong,Kim, Hye-Sun,Baik, Tai-Kyoung,Woo, Ran-Sook American Society for Biochemistry and Molecular Bi 2015 The Journal of biological chemistry Vol.290 No.33
<P>Neuregulin 1 (NRG1) is a trophic factor that is thought to have important roles in the regulating brain circuitry. Recent studies suggest that NRG1 regulates synaptic transmission, although the precise mechanisms remain unknown. Here we report that NRG1 influences glutamate uptake by increasing the protein level of excitatory amino acid carrier (EAAC1). Our data indicate that NRG1 induced the up-regulation of EAAC1 in primary cortical neurons with an increase in glutamate uptake. These <I>in vitro</I> results were corroborated in the prefrontal cortex (PFC) of mice given NRG1. The stimulatory effect of NRG1 was blocked by inhibition of the NRG1 receptor ErbB4. The suppressed expression of ErbB4 by siRNA led to a decrease in the expression of EAAC1. In addition, the ablation of ErbB4 in parvalbumin (PV)-positive neurons in PV-ErbB4<SUP>−/−</SUP> mice suppressed EAAC1 expression. Taken together, our results show that NRG1 signaling through ErbB4 modulates EAAC1. These findings link proposed effectors in schizophrenia: NRG1/ErbB4 signaling perturbation, EAAC1 deficit, and neurotransmission dysfunction.</P>
Facile One-Pot Synthesis of 1,3,5-Trisubstituted Pyrazoles from α,β-Enones
Yu, Jin,Kim, Ko Hoon,Moon, Hye Ran,Kim, Jae Nyoung Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.6
A practical and efficient one-pot synthesis of 1,3,5-trisubstituted pyrazoles from ${\alpha},{\beta}$-enones and arylhydrazine hydrochlorides has been developed. The pyrazoles were formed via a tandem formation of the corresponding pyrazolines and an acid-catalyzed aerobic oxidation process.