"Tinidazole"(Fasigyn)에 依한 Giardia Lamblia의 驅蟲效果

崔源永,李玉蘭,李元求,이규면,朴定圭,李水月 中央醫學社 1973 中央醫學 Vol.25 No.4

"Tinidazole a new antiflagellate drug, was given orally for 3-7 days to 99 children infected with G.lamblia. Stool examination for detection and evaluation of therapeutic on giardiasis were made by formaline-ether concentration techniques. The children were grouped according to doses and the duration of administration of the drug. The results were summarized as follows; 1) Except 1 case out of 99 children, none of the children showed G. lamblia in the stool examination 2 weeks after administration of the drug, regardless of groups. 2) 4 weeks after administration of the drug, group A (10 mg per kg for 7 days) and group B (5 mg per kg for 7 days) children showed 100% of negative conversion, but group C (10 mg per kg for 5 days) children revealed 84.6% of negatives. 3) 6 months later, the negative conversion rates in groups A, B and C were reduced to 54.5%, 57. 1% and 50. 0% respectively. 4) Mild or transient side effects-nausea, abdominal pain, headache or fever and diarrhea were noted in 16 cases (27. 1%) out of 59 cases.

간흡충 성충의 조직항원 부위와 충체 추출물의 항원성

이옥란,이민구 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

간흡충(Clonorchis sinensis)의 수용성추출물의 항원성과 충체조직의 부위별 조직항원의 황원성을 비교함으로써 수용성항원의 항원성을 주도하는 체조직항원부위를 알아보고자 이 실험을 시도하였다. 간흡충의 수용성항원은 충체 추출물(조항원)을 DEAE-anion exchange chromatography로 부분 정제한 분리하원(D1, D2, D3, D4 및 D5)을 사용하였고, 이들 수용성항원은 SDS-PAGE로 각 항원의 분자량별 단백분획상과 EITB로 단백분획의 항원성을 비교하였다. 조직항원의 항원성은 면역황금 표지법으로 부위별 조직항원을 검사하였다. 미세 조직항원의 면역반응 관찰에는 간흡충 감염 토끼 혈청, D3면역 토끼혈청 및 D5면역 토끼혈청을 사용하였다. 간흡충의 수용성항원과 분리항원들은 공통분획들이 관찰되었고 일부 분획들은 염색상 농도 차이를 보였다. 이들 수용성항원의 단백분획들 대부분이 항체와 결합한 것으로 보아 충체 단백물질 대부분이 항원성이 있음을 알 수 있었다. 모든 항원에서 항체와 강하게 결합된 공통 분획은 27 kDa, 16 kDa및 14 kDa분획이었다. 수용성항원과 감염 흰주혈청을 사용한 ELISA에서 D5항원은 감염초기(감염2주)를 제외한 전 감염기간 중 강력한 반응을 보였고, D1항원은 감염초기(2주)에 강한 항원성을 보였다. 면역황금표지법 상 간흡충의 표피, 장상피, 배설낭 및 난황선과 항혈청들(감염혈청, D3면역혈청및 D5면역혈청)의 반응은 강도의 차이는 있으나 항원이 있는 조직들이었다. 전 조직은 D5면역혈청반응에서 강한 항원성을 보였고 D2및 D3면역혈청에서 약한 반응을 보였다. D5면역혈청을 반응시켰을 때 난황선은 많은 황금입자가 표지되어 항원단백질이 풍부한 조직부위로 보이나 감염혈청과 조직의 면역반응에서는 난황선의 항원성은 강하지 않았다. 면역황금표지법 상 간흡충의 표피, 장상피, 배설낭 및 난황선과 항혈청들(감염혈청, D3면역혈청및 D5면역혈청)의 반응은 강도의 차이는 있으나 항원이 있는 조직들이었다. 전 조직은 D5면역혈청반응에서 강한 항원성을 보였고 D2및 D3면역혈청에서 약한 반응을 보였다. D5면역혈청을 반응시켰을 때 난황선은 많은 황금입자가 표지되어 하원단백질이 풍부한 조직부위로 보이나 감염혈청과 조직의 면역반응에서는 난황선의 항원성은 강하지 않았다. 결론적으로 간흡충의 충체추출 수용성항원은 간흡충충체 항원단백질의 혼합체로 구성되었으나 감염혈청과 면역혈청에서 강한 항체결합을 한 장상피 및 배설낭이 주 항원성 단백질함유조직으로 보이며, 이 중 가장 강한 항원성을 보인 장조직은 수용성항원의 항원성을 주도하는 조직항원부위로 생각한다. The major antigenic site in the tissue of Clonorchis sinensis were observed and compared with antigenicity of the worm extracted soluble antigens. The worm extracted crude antigen(CSWA) was separated into five protein fractions(seperate antigens: D1, D2, D3, D4 and D5) by DEAE anion exchange chromatography. These antigens were analized with SDS-PAGE(SDS-polyacrylamide gel electrophoresis) and the EITB(enzyme linked immunotransfer blot). Antigenicity of the crude and separated five antigens were evaluated by ELISA(enzyme-linked immunosorbent assay) reacted with sera from rat infected with Clonorchis sinensis. The antigenicity in tissue sites of Clonorchis sinensis was observed with electronmicroscopy by immunogold labeling method using three anti rabbit sera (the infected serum with Clonorchis sinensis, the D3 and the D5 immune rabbit sera). The results were as follows: By EITB, the most protein fractions on SDS-PAGE were binded to infected rat sera, and the strongly binding fractions against in all antigens were molecular weight of 27 kDa, 16 kDa and 14 kDa. In antigenicity estimated by the OD value of ELISA, the D5 was revealed stronger than those of the others. Statistically, the antigenicity of the D5 was stronger(p<0.001) than that of other separated antigens(p<0.05 ~ p<0.001) from 4 weeks to 20 weeks after infection. In the ultrastructural tissues, the tegumental syncytium, the intestinal epithelium, the excretory bladder and the vitellaria of Clonorchis sinensis were labeled with different density of the immunogold particles against all three antisera. The immunogold particles of a large numbers were labeled on the intestinal epithelium and the excretory bladder, but in the tegument and the vitellaria were labeled scarecely. Much more numbers of immunogold particles were labeled in D5 immune serum than other antisera, and especially, a large number of immunogold particles were labeled in intestinal epithelium. Summarizing the above results, although the antigenicity of Clonorchis sinensis was shown in all the tissues(tegument, intestine, excretory bladder and vitellaria), the major antigenic site was to be the intestinal region.

흰쥐 간장 내 일록춘폐흡충의 충낭 1례

이옥란,이선화 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

The ectopic infection except lung of Paragonimus species are frequent occurrence, but the liver rare involved as a site of ectopic Paragonimiasis. There are not reported till now for liver invasion of Paragonimus iloktsuenensis. This is a report that we have observed a case of the ectopic liver infection of P. iloktsuenensis in an experimental infected albino rat. As a rat among 1,017 rats that were experimented infection with P. iloktsuenensis during 13 years(1989~2001) in the department of Parasitology in Soonchunhyang Medical College, the rat was experimentally infected with 70 excysted metacercariae of P. iloktsuenensis through oral route, was autosied on sixth week after infection, At autopsy of the rat, 7 cysts contained two worms in each cyst was detected in lung and a cyst were detected in live as ectopic site. The cyst lesion in the liver was located inside the right lobe of the liver that was a slightly protrudent domeshaped lesion about 10mm in maximum dimensions. In excision of the cyst, two viable worms of P. iloktsuenensis was reside in well developed cyst wall that was surrounded with numerous leukocytes and thick fibrous tissues. These two worms were adult worms that were measured with size of 7×4.3mm and 9×4.1mm respectively and were well developed ovary, testis, vitellaria and uterus on Semicon's Acetocarmine staining. Two adult worms of P. iloktsuenensis in ectopic liver syst of alvino rat was developed as same as development in lung. We supposed that the route of ectopic liver infection was assumed to be a direct invasion during larval migraton from intestine to lung of albino rat.

Go Tell It on the Mountain에 나타난 John Grimes의 Identity 探究

李玉蘭 新羅大學校 1985 論文集 Vol.19 No.-

Go Tell It on the Mountain is generally understood as one of James Baldwin`s best novels as well as a major contribution to American fiction. The novel is divided into three parts. Part Ⅰ, "The Seventh Day", Part Ⅱ, "The Prayers of the Saints" and PartⅢ, "The Threshing Floor". The novel deals with the Negro not as a social problem or as an oppressed minority, but concentrates almost exclusively on one aspect of him and his way of life-the religious. The particular kind of religion which is grows out of his traditional situation in America and especially of his experience of slavery. Baldwin renders the service with great skill which begins on Saturday evening and continues into the small hours. Through the Grimeses` prayers we learn the past lives of Gabiel, his wife, Elizabeth, his sister, Florence, and his step-son, John in a series of flashbacks: a whole intricate dramatic pattern of sin and guilt and repentance underlying their lives is revealed. And the novel ends with John`s experience of conversion. The central event of this novels is the religious conversion of fourteen-year-old John chiefly around whom all the other characters` difficulties are centered. The writer endeavors here not only to interconnect the lives and psychology of all the characters but also to relate these to the Southern Negro experience and the consequent shocks of urban slum living. As Gabriel`s story is told through his memory, it is clear that "Father" and "Son" refer not only to God and Jesus Christ, but to the sons Gabriel fathers and rejects, adopts and hates, fathers and finds disappointing. In John, the innocent victim of Gabriel` hatred, we have an archetypal image of the Negro child. Baldwin sees the negro quite literally as the bastard child American civilization. Obliquely, by means of an extended metaphor, Baldwin approaches the very essence of Negro experience. That essence is rejection, and its most destructive consequence is shame. The flight from Negro self, the quest for identity, and the sophisticated acceptance of one`s "blackness" is the theme that flow this emotion. That is, the wirter presents the Negro`s inmost conflicts, his distress and his guilts from the past to the present and make attempt to discover the meaning of Negro`s subsistence and the possibility of achieving Negro`s identity as a human being. PartⅢ, "The Threshing Floor", as John lies before the altar, a series of visionary states passes through his soul. Images of darkness ane chaos, silence and emptiness, mist and cold. These images of damnation express the state of the soul when thrust into outer darkness by a rejecting, punishing, castrating father figure who is the surrogate of a hostile society. At the depth of John`s despair, a sound emerges to assuage his pain. He had heard it all his life, but it was only now that his ears were opened to this sound that come from the darkness, that yet bore such witness to the glory of the light. And now in his moaning, he heard it in himself. It rose from his bleeding, his cracked-open heart. It was a sound of rage and weeping from time set free, but bound now in eternity. John`s fear and guilt, and desire and despir and hatred have all been converted into a kind of meaningful delirium as he lies thrashing about the floor. On these harsh terms, Baldwin`s protagonist discovers his identity. He belongs to those armies of darkness and must forever share their pain. To the question, Who am I?, he can now reply: I am he who suffers and yet whose suffering on occasion is "from time set free". And thereby he discovers his humanity. We are very close to that plane of human experience where art and religion intersect. What Baldwin wants us to feel is the emotional pressure exerted on the Negro`s cultural forms by his exposure to white oppression. And finally to comprehend that these forms alone, through their power of transforming suffering, have enabled him to survive his tragic conditions of existence. Moreover, John`s struggle on the threshing floor is described in terms of birth imagery, and the accomplished delivery set him free from the womb of childhood. That is, Baldwin anticipates in new-born John who seems to be a true Negro image of the new type in the future, and also aspires to be an American society which the whites and the black can co-exist without the racial prejudice.

초등학교 5. 6 학년의 비만도에 따른 스트레스·자아존중감 조사 연구

이은주,김옥란,신영희 김천과학대학 2003 김천과학대학 논문집 Vol.29 No.-

This paper is a descriptive study that inquires about stress and self-esteem of obese children to lend assistance about understanding emotional care as weight management of children. The data collection was given by the structured questionnaire during five days, from 5th to 10th on march in 2003 at one primary school located in K-city. The subjects were 286 children in 11-12 year old children. The instruments used for this study were an Obesity-Stress Scale(Cho Yang Rae, 1997) and a self-esteem questionnaire including Self-Perception Profile(Harter, 1982). Data were analyzed with frequency, percentages, x²-test, ANOVA, Pearson correlation using the SPSS program. The results were as follows; 1. A stress of obese children was the highest among three group, which was significant of statistical data. On the other hand, self-esteem do be shown the same as the above but was not significant statistically. 2. A relation between stress and self-esteem of obese children was shown to be in inverse proportion ratio so that the higher stress was the lower self-esteem. 3. To research the differences of the stress followed by the general characteristics of children, there are meaningful differences in sex, character, factor of stress, shape of father's body and shape of mother's body. 4. To research the differences of the self-esteem followed by the general character of children, there are meaningful differences in sex, character, shape of mother's body and socioeconomic status.

협력학습을 통한 실어증자의 그룹치료 효과

이옥분,정옥란,고도흥 한국음성과학회 2004 음성과학 Vol.11 No.2

This study attempted to determine the effects of a cooperative and cognitive group therapy compared to individual therapy in 24 aphasic subjects. Two dependent variables were measured overall language performance, functional communication skills. 18 subjects with different types and severity of aphasia participated in the group therapy. 6 aphasic subjects participated in the individual therapy and they functioned as a control group. The subjects were ranged from 27 to 59 years in age. The group therapy using the cooperative learning utilized the following procedures. First, 6 aphasics constituted 1 group where each subject performed a task and they monitored one another. Second, 2 aphasics consisted 1 group and they cooperated to perform a task. Third, 3 groups with 2 aphasics in a group competed one another in a task where the 2 aphasics had to cooperated. Finally, the investigator gave the feedback to the group and she and the subjects discussed the overall procedures of the therapy. The above mentioned 2 tests were administered pre- and post-treatment. A repeated two-way ANOVA was performed for analysis. The results showed that the group therapy was more effective in improving overall language performance as compared to the individual therapy. And, the group therapy was more effective in increasing functional communication skills as compared to the individual therapy.

일록춘폐흡충 항원성분에 단백질분해효소의 영향 1. 단백질분해효소억제제 첨가 일록춘폐흡충 항원성분

이민구,이옥란,남해선 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.1

We were study for influence of proteinase in adult worm extract antigenic protein components of Paragonimus iloktsuenensis by use of proteinase inhibitor, E-64 ([L-3-transcar-boxyoxiran-2-carbonyl]-L-leucyl-agmatin). Examined antigens were no-added antigens with proteinase inhibitor(E-64) and added antigens with E-64, and these antigenic proteins were separated by SDS-PAGE. The results are summarized as follows : Added antigens were more made up 5 to 20 protein(polypeptide) bands in molecular weight from 40Kda to 200Kda than no-added antigens. Especially, stain density of protein bands were strong markedly on molecular weight 45Kda, 60Kda and 83Kda in added antigens compared with no-added antigens. On the other hand, in molecular weight level below 40Kda, there were similar numbers or stain density of protein bands between added antigens and no-added antigens, but the protein band of 32Kda was more strong stain density in no-added antigens than in added antigens. In conclusion, adult worm extract antigens of P. iloktsuenensis were affected by intrinsic proteinase of P. iloktsuenensis in SDS-PAGE. There are regarded as the protein component of molecular weight 45Kda was affected most by proteinase in SDS-PAGE, and the protein component of molecular weight 32Kda was affected partially by proteinase inhibitor.

경기도 안성군내 초등학교 학생들의 요충감염 양상

이선화,이옥란,남해선 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.1

We examined the prevalence rate of Enterobius vermicularis infection by single examination of cellotape anal swap for 658 children of 4 primary schools and attatched kindergartens in Ansung of Korea in June, 1996. The total positive rate was 12.9%, and that of female children(15.9%) was higher than male(10.0%). Treatment was achieved by three different ways with albendazole 400 mg as a total. Six months later, follow-up examination was done. Overall egg positive rate of follow-up study was 6.8%. The change of egg positive rates between first and follow-up examination of each group were as follows: 1. A group of single medication for egg positive children only : 13.3% to 7.5% (reduction rate, 43.6%). 2. A group of single medication for all individuals in a class : 9.2% to 4.6% (reduction rate, 50.0%). 3. A group of twice medication at an interval of 20 days for all individuals in a class : 19.3% to 8.6% (reduction rate, 55.4%). These results suggest that medication of albendazole of every six months may be reduce the egg positive rate of Enterobius vermicularis remarkably, especially the group that has relatively low prevalence rate of enterobiasis.

일록춘폐흡충의 실험적 연구 : 1. 탈낭유충 섭취에 의한 흰쥐 감염율 1. Infection Rates in Albino Rats by feeding of Excysted Larvae.

이옥란 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

The infection rates and the recovery rates of Paragonimus iloktsuenensis were examined in experimentally infected albino rats. Excysted larvae of P. iloktsuenensis were administered 34 times to albino rats in various doses(34 to 100 excysted larvae per rat) during the ten year period of 1989 and 1998. They were detected as worms in the lung at two to 36 weeks after infection. The infection rates of the albino rats were calculated as the ratio between rats with worms in the lung and rats without worms in the lung. The recovery rates of the worms were calculated as the number of worms found in the rats over the number of excysted larvae. The results were as follows: 1. The infection rates and the recovery rates are shown in Table 1. The average infection rate of all groups was 72.6%(range: 60% to 90.6%). The mean number of worms recovered per experimental rat was 5.4(range: 1.9 to 10.6), and the mean recovery rate was 9.0%. 2. The infection and recovery rates demonstrated marked differences according to the times of infection. The mean recovery rate was in the range of 4.3∼20.9(mean: 9.0%)

Go Tell It on the Mountain에 나타난 John Grimes의 Identity 探求 : Go Tell It on the Mountain

李玉蘭 동아대학교 인문과학대학 영어영문학과 1990 동아영어영문학 Vol.6 No.-

Go Tell It on the Mountain is generally understood as one of James Baldwin's best novels as well as a major contribution to American fiction. The novel is divided into three parts. Part Ⅰ, "The Seventh Day", Part Ⅱ, "The Prayers of the Saints" and part Ⅲ, "The Threshing Floor" The novel deal with the Negro not as a social problem or as an oppressed minority, but concentes almost exclusively on one aspect of him and his way of life-the religious. The particular kind of religion which is grows out of his traditional situation in America and especially of his experirce of slavery. Baldwin renders the service with great skill which begins on Saturday eveninH and continues into the small hours. Through the Grimeses' prayers we learn the past lives of Gabiel, his wife, Elizabeth, his sister, Florence, and his step-son, John in a series of flashbacks : a whole intricate dramatic pattern of sin and guilt and repentance underlying their lives is revealed. And the novel ends with John's experience of conversion. The central event of this this novel is the religiour conversion of fourteen-year- old John but the truly major figure of interest is John's father, Gabriel- and it is Gabriel, chiefly around whom all the othe rcharacters' difficulties are centered. The writer endeavors here not only to interconnect the lives and psychology of all the characters but also to related these to the Southern Negro experience and the consequent shocks of urban slum living. As Gabriel's story is told through his memory, it is clear that "Father" and "Son" refer not only to God and Jesus Christ, but to the sons Gabriel fathers and rejects, adopts and hates, fathers and finds disappointing. In John, the innocent victim of Gabrie'l hatred, we have an, archetypal image of the Negro child. Baldwin sees the negro quite literally as the bastard child of American civilization. Obliquely, by means of an extended metaphor, Baldwin approaches the very essence of Negro experience. That essence is rejection, and its most destructitre consequence is shame. The flight from Negro self, the quest for identity, and the sophisticated acceptance of one's "blackness" is the theme that flow this emotion. That is, the wirter presents the Negro's inmost conflicts, his distress and his guilts from the past to the present and make attempt to discorer the meaning of Negro's subsistence and the possibilily of achieving Negor's identity as human being. In Part Ⅲ, "The Threshing Floor", as lohn lies before the altar, a series of visionary states passes through his soul. Images of darkness ane chaos, silence and emptiness, mist and cold. These images of damnation exprress the state of the soul when thrust into outer darkness by a rejecting, punishing, castrating father figure who is the surrogate of a hostile society. At the depth of John's despair, a sound emerges to assuage his pain. He had heard it all his life, but it was onlynow that his ears were opened to this sound that come from the darkness, that yet bore such witness to the glory of the light. And now in his moaning, he heard it in himself. It rose from his bledding, his cracked-open heart. It was a sound of rage and weeping from time set free, but bound noir in eternity. John's fear and guilt, and desire and despir and hatred have all been converted into a kind of meaningful delirium as he lies thrashing about the floor. On these harsh darkness and must forever share their pain. To the question, Who am I?, he can now reply : I am he who suffers and yet whose suffering on occasion is "from time set free". And thereby he discovers his humanity. We are very close to that plane of human experience where art and religion intersect. What Baldwin wants us to feel is the emotional pressure exerted on the Negro's cultural forms by his exposurc to white oppression. And finally to comprehend that these forms alone, through their power of transforming suffering, have enabled him to survive his tragic conditions of existence. Moreover, John's struggle on the threshing floor is described in terms of birth imagery, and the accomplished delivery set him free from the womb of childllood. That is, Baldwin anticipates in new -born John who seems to be a true Negro image of the new type in the futture, and also aspires to be an American society which the whites and the black can co-exist whithout the racial prejudice.