Disruption of endothelial barrier function is linked with hyposecretion and lymphocytic infiltration in salivary glands of Sjögren's syndrome

Cong, Xin,Zhang, Xue-Ming,Zhang, Yan,Wei, Tai,He, Qi-Hua,Zhang, Li-Wei,Hua, Hong,Lee, Sang-Woo,Park, Kyungpyo,Yu, Guang-Yan,Wu, Li-Ling Elsevier 2018 Biochimica et biophysica acta. Molecular basis of Vol.1864 No.10

<P><B>Abstract</B></P> <P>Sjögren's syndrome (SS) is an inflammatory autoimmune disease that causes hyposecretion in salivary glands. Endothelial tight junctions (TJs) play crucial roles in salivation and barrier function of blood vessels. However, whether the alteration of endothelial TJs were involved in pathogenesis of SS was still unknown. Here, the ultrastructure and function of endothelial TJs in submandibular glands (SMGs) were detected by transmission electron microscopy and in vivo paracellular permeability assay in different aged NOD mouse model for SS. CFSE-labeled lymphocytes were injected into tail vein to trace the infiltration, while claudin-5 expression and distribution were detected by immunofluorescence, qRT-PCR, and western blot. Results showed that the stimulated salivary flow rate was gradually decreased and lymphocytic infiltration was found as age increased in 12- and 21-week-old NOD mice, but not 7-week-old NOD mice. Blood vessels were dilated, while endothelial TJ width and paracellular tracer transport were increased in 12-week-old NOD mice. Moreover, the injected CFSE-labeled lymphocytes were observed in SMGs of 12-week-old NOD mice. Claudin-5 level was increased and relocalized from the apical portion of neighboring endothelial cells to lateral membranes and cytoplasm in 12-week-old NOD mice. Additionally, the alteration of claudin-5 expression and distribution was further confirmed in labial salivary glands and bilateral parotid glands from SS patients. In cultured human microvessel endothelial cell line (HMEC-1), IFN-γ stimulation significantly increased claudin-5 expression. Taken together, we identified that the endothelial TJ barrier was disrupted and contributed to the development of salivary hyposecretion and lymphocytic infiltration in SS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Endothelial tight junction barrier is disrupted in hyposecretory submandibular glands from Sjögren's syndrome mouse model </LI> <LI> The disrupted salivary endothelial barrier is linked with lymphocytic infiltration in Sjögren's syndrome mouse model </LI> <LI> The redistribution of claudin-5 is responsible for disrupted endothelial barrier in salivary glands from Sjögren's syndrome </LI> </UL> </P>

Association between Pax8-PPARγ1 Rearrangement and Follicular Thyroid Cancer: a Meta-Analysis

Li, Hang-Yu,Xie, Zhi-Hao,Xu, Cong-Hui,Pu, Mei-Ling,Chen, Zi-Yan,Yu, Miao,Wang, Heng-Shu,Zhou, Chen-Ming,Pu, Chao-Yu,Liu, Wei Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9

Background: Pax8 and peroxisome proliferator-activated receptor gamma 1 gene (Pax8-$PPAR{\gamma}1$) are important factors in tumors. Several studies have suggested that follicular thyroid cancer may arise from Pax8- $PPAR{\gamma}1$ rearrangement. In order to have a better understanding of the association between Pax8-$PPAR{\gamma}1$ rearrangement and follicular thyroid cancer, we conducted the presenmt meta-analysis. Materials and Methods: The information was extracted from PubMed, EMBASE and Web of Science. Statistic analysis was performed with Stata12.0 software. Odds ratios (ORs) were calculated using a fixed-effects model. We also performed heterogeneity and publication bias analyses. Results: Nine studies including 198 follicular thyroid cancer patients and 268 controls were considered eligible. The frequency of Pax8-$PPAR{\gamma}1$ rearrangement was significantly higher in the follicular thyroid cancer group than in the control group, with a pooled OR of 6.63 (95%CI=3.50-12.7). In addition, through subgroup analysis, the OR between Pax8-$PPAR{\gamma}1$ rearrangement and follicular thyroid cancer was 6.04 (95%CI = 3.18-11.5) when using benign tumor tissues as controls. The OR for the method subgroup was 9.99 (95% CI =4.86-20.5) in the RT-PCR. Conclusions: The final results demonstrated that Pax8-$PPAR{\gamma}1$ rearrangement has significant association with follicular thyroid cancer.

Analysis on the change of total factor productivity of wheat in China and its influencing factors

Yu-Cong Sun,Shuang-Yu Hu,Sung-Chan Kim,Zhi-Run Li,Jong-In Lee 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.9

밀은 중국의 3대 식량작물의 하나로서 생산량은 사회생활과 산업발전에 중요한 역할을 한다. 본 연구에서 중국 12개 밀 주요 생산지역을 대상으로 중국의 농산물 비용 및 수익의 데이터를 통하여 2005년부터 2019년까지 DEA-Malmquist 모델을 이용하여 밀의 총요소 생산성을 측정하였으며, 또한 중국 통계연감, 교통 연감, 중국 농촌 통계 연감의 데이터를 기반으로 LSDVC 모형을 이용하여 효율성은 영향요인을 측정하였다. 분석결과, 2005년부터 2019년까지 중국 19년간 밀의 총요소 생산성은 내림세를 보였고 연평균 증가율은 –1.5%였다. 밀의 생산 효율성 영향요인을 보면, 농업의 기계화동력은 밀의 총요소생산성에 뚜렷한 긍정적(+)인 영향을 주지만 정부의 농업 공공지출, 산업화는 밀의 총요소생산성에 뚜렷한 부정적(-)인 영향을 준다. 또한 농촌의 도로밀도와 평균 기온과 도시화는 밀의 생산성 요소의 영향이 뚜렷하지 않다. 밀 생산력의 지속적인 향상을 위해 앞으로 밀 산업의 발전은 농업 발전방식을 중점적으로 전환하고 인력 투입을 확대하며 공업 부문의 농업 육성을 장려해야 한다. As one of the three major food crops in China, wheat production plays a vital role in people’s lives and industrial development. This study used a DEA-Malmquist model to calculate and analyze the wheat total factor productivity (TFP) of 12 major wheat-producing provinces from 2005 to 2019. The data necessary for this calculation were obtained from the “National Farm Product Cost-benefit Survey”. Further to this calculation, an LSDVC model was used to explore the factors influencing the TFP. For this, the data were obtained from the “China Statistical Yearbook”, “Rural Statistical Yearbook of China”, and “Transport Yearbook”. The results showed that from 2005 to 2019, China’s wheat total factor productivity showed a decline and increased at an average annual rate of -1.5%. As far as the factors influencing the wheat production efficiency are concerned, the total mechanical power has a significant positive impact on the TFP of wheat. In contrast, government expenditures for agriculture, forestry and water resources, and industrialization have significantly adverse effects on the wheat TFP. Moreover , the rural road density and urbanization showed no significant impact on the TFP of wheat. Therefore, to promote the sustainable improvement of wheat productivity, the future development of the wheat industry should focus on the transformation of the agricultural development mode, human resources investment, and encourage the industry sector to nurture agriculture.

Prognostic Role of Hypoxic Inducible Factor Expression in Non-small Cell Lung Cancer: A Meta-analysis

Li, Cong,Lu, Hua-Jun,Na, Fei-Fei,Deng, Lei,Xue, Jian-Xin,Wang, Jing-Wen,Wang, Yu-Qing,Li, Qiao-Ling,Lu, You Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Introduction: Reported prognostic roles of hypoxic inducible factor (HIF) expression in non-small cell lung cancer (NSCLC) have varied. This meta-analysis aimed to examine the relationship between HIF expression and clinical outcome in NSCLC patients. Methods: PubMed were used to identify relevant literature with the last report up to December $20^{th}$, 2012. After careful review, survival data were collected from eligible studies. We completed the meta-analysis using Stata statistical software (Version 11) and combined hazard ratio (HR) for overall survival (OS). Subgroup specificity, heterogeneity and publication bias were also assessed. All of the results were verified by two persons to ensure accuracy. Results: Eight studies were finally stepped into this meta-analysis in which seven had available data for HIF-$1{\alpha}$ and three for HIF-$2{\alpha}$. Combined HRs suggested that higher expression of $HIF1{\alpha}$ had a negative impact on NSCLC patient survival (HR=1.50; 95%CI=1.07-2.10; p=0.019). The expression of HIF-$2{\alpha}$ was also relative to a poorer survival (HR=2.02; 95%CI=1.47-2.77; p=0.000). No bias existed in either of the two groups. Conclusion: This study suggests that elevations of HIF-$1{\alpha}$ and HIF-$2{\alpha}$ expression are both associated with poor outcome for patients with NSCLC. The data support further and high quality investigation of HIF expression for predicting poor outcome in patients with NSCLC.

Study on the effect of minimum purchase price policy on China’s wheat production

Yu-Cong Sun(손우총),Shuang-Yu Hu(호쌍우),Li Yang(양리),Sung-Chan Kim(김성찬),Jong-In Lee(이종인) 한국산학기술학회 2022 한국산학기술학회논문지 Vol.23 No.2

Study on the influencing factors of wheat import trade in China

Yu-Cong Sun(손우총),Shuang-Yu Hu(호쌍우),Zhi-Run Li(이지윤),Ji-Yeon Shim(심지연),Jong-In Lee(이종인) 한국산학기술학회 2022 한국산학기술학회논문지 Vol.23 No.2

The Catechol-O-Methyltransferase Val158Met Polymorphism Contributes to the Risk of Breast Cancer in the Chinese Population: An Updated Meta- Analysis

Guo-Xing Wan,Yu-Wen Cao,Wen-Qin Li,Yu-Cong Li,Feng Li 한국유방암학회 2014 Journal of breast cancer Vol.17 No.2

Purpose: Catechol-O-methyltransferase (COMT) enzyme plays acentral role in estrogen-induced carcinogenesis. Emerging evidencefrom association studies has revealed that the functionalVal158Met polymorphism (rs4680 G>A) of the Catechol-Omethyltransferasegene (COMT) has been implicated in susceptibilityto breast cancer in the Chinese population, while resultsof individual published studies remain inconclusive and inconsistent. To assess this association in the Chinese population, a meta-analysis was performed. Methods: Eligible studies weresearched on MEDLINE, Embase, Cochrane Library, China NationalKnowledge Infrastructure, and the Chinese BiomedicineDatabase. Odds ratios (ORs) with their corresponding 95% confidenceintervals (CIs) were pooled to assess the association betweenCOMT polymorphisms and the risk of breast cancer usingRevMan 5.2 and Stata 12.0 software. Results: The meta-analysisincluded 14 eligible studies, with a total of 4,626 breast cancercases and 5,637 controls. Overall, the COMT Val158Met polymorphism(rs4680 G>A) was significantly associated with an increasedrisk of breast cancer in several genetic models (A/A vs. G/G: OR, 1.59, 95% CI, 1.12–2.27; A/A vs. G/A+G/G: OR, 1.62,95% CI, 1.14–2.29; A vs. G: OR, 1.15, 95% CI, 1.00–1.32), and asubgroup analysis according to menopausal status showed thatthis association was especially evident among premenopausalChinese women (A/A vs. G/G: OR, 1.87, 95% CI, 0.99–3.54; A/Avs. G/A+G/G: OR, 1.94, 95% CI, 1.03–3.63). Conclusion: The resultsof this meta-analysis indicated that COMT Val158Met variantscontribute to breast cancer susceptibility in the Chinesepopulation, particularly among premenopausal women.

Associations Between TLR9 Polymorphisms and Cancer Risk: Evidence from an Updated Meta-analysis of 25,685 Subjects

Wan, Guo-Xing,Cao, Yu-Wen,Li, Wen-Qin,Li, Yu-Cong,Zhang, Wen-Jie,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

A meta-analysis incorporating 34 case-control studies from 19 articles involving 12,197 cases and 13,488 controls was conducted to assess the effects of three genetic variants of Toll-like receptor 9 (TLR9): rs187084, rs352140, and rs5743836. Studies on associations between TLR9 polymorphisms and cancer risk were systematically searched in electronic databases. The reported odds ratios (OR) and 95% confidence intervals (CI) were pooled to assess the strength of any associations. The results showed that the rs187084 polymorphism was significantly associated with an increased risk of cancer (CC vs TC+TT: OR=1.14, 95% CI=1.02-1.28), specifically cervical cancer (C vs T: OR=1.19, 95% CI=1.05-1.34; TC vs TT: OR=1.32, 95% CI=1.10-1.58; CC vs TT: OR=1.31, 95% CI=1.03-1.68; CC+TC vs TT: OR=1.32, 95% CI=1.11-1.56), and that this association was significantly positive in Caucasians (CC vs. TC+TT: OR=1.18, 95% CI=1.01-1.38). The rs352140 polymorphism had a protective effect on breast cancer (GA vs GG: OR=0.77, 95% CI=0.66-0.89), whereas the rs5743836 polymorphism was likely protective for digestive system cancers (CC+TC vs TT: OR=0.81, 95% CI=0.66-0.98). In conclusion, our results suggest that the rs187084 polymorphism may be associated with an elevated cancer risk, whereas polymorphisms of rs352140 and rs5743836 may play protective roles in the development of breast and digestive system cancers, respectively. From the results of this meta-analysis further large-scale case-control studies are warranted to verify associations between TLR9 polymorphisms and cancer.

High-j Proton h11/2 and g7/2 Intruder Bands in 113In

Ma Ke Yan,Lu Jing Bin,Ma Ying Jun,Li Jian,Yang Dong,Sun Wu Ji,Wang Hao,Pan Hao Nan,Wang Jia Qi,Yang Qing Yu,Zhang Da Ming,Zhu Li Hua,Wu Xiao Guang,Zheng Yun,Li Cong Bo 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.76 No.12

Excited states of 113In have been populated through the heavy-ion fusion evaporation reaction 110Pd(7Li, 4n)113In. A new band with the configuration of a proton d5/2 orbital is identified. Two ΔI = 2 intruder bands, built on the πh11/2 and the πg7/2 orbitals, have been extended to spins (63/2-)ħ and (55/2+)ħ, respectively. The negative-parity πh11/2 intruder band shows a smooth increase in aligned spin, which is attributed to a strong proton-neutron interaction. The properties of the positive-parity πg7/2 band are discussed based on tilted axis cranking model calculations, and the features of the antimagnetic rotation for this band are shown after backbend. Furthermore, the contributions of the two-shears-like mechanism, the neutron (gd)ν shell and the core rotation are investigated for the positive-parity πg7/2 band.

Schisantherin A Improves Learning and Memory of Mice with D-Galactose-Induced Learning and Memory Impairment Through Its Antioxidation and Regulation of p19/p53/p21/ Cyclin D1/CDK4/RB Gene Expressions

Cong Liu,Weijing Sun,Ning Li,Jiaqi Gao,Chunyan Yu,Chunmei Wang,Jinghui Sun,Shu Jing,JianGuang Chen,He Li 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.7

Schisantherin A (SCA) was evaluated for possible function in restoring the learning and memory impairment induced by D-galactose in mice. ICR mice were treated with D-galactose subcutaneously (220 mg·kg−1), and followed by SCA in different doses (1.25, 2.50 and 5.00 mg·kg−1, administered orally) for 42 days. Effects of SCA on learning and memory were examined by step-through tests and Morris water maze tests. The activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) in the peripheral blood and hippocampus of mice were assayed by water-soluble tetrazolium-1 (WST-1) and thiobarbituric acid (TBA) methods. The contents of 8 hydroxy deoxy guanosine (8-OHdG) in the hippocampus of mice were detected by immunosorbent assay methods, respectively. Quantitative real-time PCR and Western Blot were respectively used to detect the expression of p19, p53, p21, cyclin D1, CDK4 and RB genes, and the phosphorylation of RB in the hippocampus of mice. We found that SCA significantly improved the learning and memory impairment induced by D-galactose in mice. After SCA treatment, SOD activity was increased and the content of MDA was decreased in both peripheral blood and hippocampus of mice. 8-OHDG content was also decreased in the hippocampus of mice. Furthermore, the expression of p19, p53 and p21 genes was reduced and the expression of cyclin D1 and CDK4 and the phosphorylation of RB protein were elevated in the hippocampus. SCA may improve the learning and memory impairment induced by D-galactose by enhancing the antioxidant capacity, and regulating the expression of p19/p53/p21/cyclinD1/CDK4 genes, and the phosphorylation of RB protein in the hippocampus of mice.