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( Yu Rim Lee ),( Eunhye Lee ),( Se Young Jang ),( Won Young Tak ),( Young Oh Kweon ),( Bina Jung ),( Gyoun Eun Kang ),( Sang Kyung Seo ),( Jung Gil Park ),( Hye Won Lee ),( Young Seok Han ),( Jae Min 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Non-alcoholic fatty liver disease (NAFLD) can progress to advanced liver disease, but only in a minority of patients. Differential liver DNA methylation of peroxisome proliferator-activated receptor gamma (PPARγ) gene promoter has been shown to distinguish patients in terms of fibrosis severity in NAFLD. However, a study of methylation of PPARγ gene promoter in human with a sufficient number of patients is still scarce. We therefore determined the prognostic significance of DNA methylation of PPARγ gene promoter in patients with NAFLD. Methods: This study enrolled 54 biopsy proven NAFLD patients and 18 healthy controls who attended Kyungpook National University Hospital, Republic of Korea between March 2015 and October 2016. We extracted genomic DNA from liver tissue of enrolled patients. Bisulfite modification of genomic DNA was performed and PPARγ methylation level was confirmed with pyrosequencing. Results: The average of 4CpG methylation of PPARγ promoter had significantly lower in the NAFLD group (15.03% DNA methylation) when compared to the healthy controls (19.55%) (P=0.001). Quantitative DNA methylation of PPARγ stratified patients into mild (Kleiner 0-2) and severe (Kleiner 3-4) fibrosis (14.21% vs 17.87%, P=0.007). Moreover, hypermethylation at the PPARγ promoter of liver was also associated with higher age, the presence of DM, NAFLD fibrosis score, the degree of steatosis and fibrosis (P=0.015, 0.024, 0.012, 0.027, 0.009, respectively). However, they were not associated with presence and degree of inflammation, ballooning, NAFLD activity score, the presence of NASH, and other liver function test. Patients with advanced fibrosis exhibited significantly higher NAFLD fibrosis score, FIB-4, and PPARγ DNA methylation (all P<0.05) and showed borderline significance with transient elastography (P=0.084). These markers, including NAFLD fibrosis score, FIB- 4, and PPARγ DNA methylation, had an area under the receiver operating curve (AUROC) of 0.855, 0.778, 0.75, respectively for predicting advanced fibrosis. PPARγ mRNA was also related to the fibrosis and steatosis severity (P<0.05). This showed negative regulation with DNA methylation level (P=0.001). Conclusions: DNA methylation level of PPARγ may be useful in NAFLD patients for stratification and prediction of progressive liver fibrosis, which is a very important factor of NAFLD patients that is currently impossible to predict. It may be possible to generate an algorithm that can predict more precisely which patients are likely to progress on to a severe fibrosis in patients with NAFLD using DNA methylation and other markers related to liver fibrosis.
Circulating Exosomal LncRNA-ATB Promotes Myopenia in Human Hepatocellular Carcinoma
( Yu Rim Lee ),( Gyeonghwa Kim ),( Won Young Tak ),( Se Young Jang ),( Young Oh Kweon ),( Jung Gil Park ),( Min Kyu Kang ),( Hye Won Lee ),( Young Seok Han ),( Jae Min Chun ),( Keun Hur ),( Soo Young 대한간학회 2021 춘·추계 학술대회 (KASL) Vol.2021 No.1
( Yu Rim Lee ),( Kyunghwa Kim ),( Se Young Jang ),( Won Young Tak ),( Young Oh Kweon ),( Bina Jung ),( Gyoun Eun Kang ),( Sang Kyung Seo ),( Jung Gil Park ),( Hye Won Lee ),( Young Seok Han ),( Jae Mi 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Long interspersed nuclear element-1 (LINE-1) hypomethylation, representing global DNA methylation level, is associated with prognosis via activation of oncogenic functions of genes. This experiment was performed to evaluate prognostic implication of LINE-1 methylation in patients with hepatocellular carcinoma (HCC) and the possible mechanisms related to oncogene activation. Methods: Ninety-six HCC patients between October 2014 and September 2015 at Kyungpook National University Hospital, Daegu, South Korea were enrolled for this prospective study. Quantitative pyrosequencing was performed to quantify the methylation level of for CpG sites in the LINE-1 promotor. The expression of CD133 and ST18 were measured by immunohistochemistry and their correlation with LINE-1 methylation levels were analyzed. Results: LINE-1 was significantly hypomethylated in tumor tissues compared with nontumor tissues (64.0 ±11.6% vs. 75.6 ±4.0%, respectively, P<0.0001). In this study population, LINE-1 hypomethylation group had a large proportion of female gender, smaller tumor size, and nonexistence of ascites (P< 0.05). Contrary to previous reports, LINE-1 hypomethylation was not an independent risk factor for overall survival and disease progression (all P >0.05). A total of 81 (84.4%) patients had demethylation of LINE-1 (ΔMI<0), and 15 (15.6%) patients had hypermethylation of LINE-1 (ΔMI≥0). HCC with demethylation of LINE-1 (ΔMI<0) had higher CD 133 expression than HCC with hypermethylation of LINE-1 (ΔMI≥0) (P=0.011). Moreover, when patients divided into two groups based on the mean value of tumor line-1 methylation, ST18 showed borderline significance in distinguishing the LINE-1 hypomethylation group than the other (P=0.053). Conclusions: LINE-1 demethylation is associated with expression of CD133 and ST18 in Hepatocellular carcinoma. In this study, LINE-1 hypomethylation was not related to overall survival and disease progression, this is probably due to the enrollment of HCC patients with various tumor stages and liver function.
Behaviour alteration of Topoisomerase IIIβ knockout mice
Yu Rim Kim,Eun Kyoung Kim,Young Sub Byun,Hae Rim Kim,Ju Sung Kang,Ju Young Lee,Min Young Lee,Sang Mi Cho,Ki Hoon Lee,Hoyoung Ghang,Won Kee Yoon,Young Suk Won,Hyoung Chin Kim,Young Hee Lee,Ki Hoan Nam 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8
Discarded Egg Yolk as an Alternate Source of Poly(3-Hydroxybutyrate-co-3-Hydroxyhexanoate)
( Yun-gi Hong ),( Yu-mi Moon ),( Ju-won Hong ),( Tae-rim Choi ),( Hye-rim Jung ),( Soo-yeon Yang ),( Dae-won Jang ),( Ye-rim Park ),( Christopher J. Brigham ),( Jae-seok Kim ),( Yoo-kyung Lee ),( Yung 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3
Many poultry eggs are discarded worldwide because of infection (i.e., avian flu) or presence of high levels of pesticides. The possibility of adopting egg yolk as a source material to produce polyhydroxyalkanoate (PHA) biopolymer was examined in this study. Cupriavidus necator Re2133/pCB81 was used for the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) or poly(3HHx), a polymer that would normally require long-chain fatty acids as carbon feedstocks for the incorporation of 3HHx monomers. The optimal medium contained 5% egg yolk oil and ammonium nitrate as a nitrogen source, with a carbon/nitrogen (C/N) ratio of 20. Time course monitoring using the optimized medium was conducted for 5 days. Biomass production was 13.1 g/l, with 43.7% co-polymer content. Comparison with other studies using plant oils and the current study using egg yolk oil revealed similar polymer yields. Thus, discarded egg yolks could be a potential source of PHA.
Rhbdf2 null mutation alleviate the severity of collagen-induced arthritis in mice
Min-Young Lee,Yong-Sub Byun,Eun-Kyoung Kim,Hae-Rim Kim,Yu-Rim Kim,Ju-Young Lee,Sang-Mi Cho,Ho-Young Ghang,Ki-Hoon Lee,Ok-Seung Moon,Young-Won Seo,Won-Kee Yoon,Young-Suk Won,Hyoung-Chin Kim,Kyung-Chul 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.2