생소한 HBsAg 선별검사키트 bioelisa와 IEMA Well의 평가

김덕언,김홍석,김신규,이혜림,박일규,강정옥,최태열 대한임상검사정도관리학회 1997 臨床檢査와 精度管理 Vol.19 No.2

Optimal media for selective isolation of entomopathogenic fungi

Tae Young Shin,Sung Min Bae,Jae Bang Choi,Ye Rim Cha,Hyun Na Koom,Soo Dong Woo 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

In agricultural fields, the entomopathogenic fungal species have been investigated for their potential as the biological control agents due to their role of natural enemies for insects. Until recent times, most of the studies for these fungi have been based on isolation from insect cadaver or soil. However, these methods, especially isolation from soil, might cause a problem involving differential isolation of the each entomopathogenic fungi. The purpose of this study is to determine the optimal isolation medium for entomopathogenic fungi using dodine, chitin, and skim milk. The growth rates of entomopathogenic fungi and non-entomopathogenic fungi were compared on dodine agar medium. The medium for this experiment was modified Veen semiselective medium which consisted of based on SDA (Sabouraund Dextrose Agar), 100 mg/ml for chloramphenicol, 50 mg/ml for streptomycin and adjusted dodine to 40, 50, 70 and 100 mg/ml. As a result, optimal concentration of dodine for isolation of entomopathogenic fungi was 50 mg/ml and 168 positive entomopathogenic fungi were isolated in 470 soil samples and 11 cadavers of insect. In addition, the isolates had significantly greater chitinase and protease activity than non-entomopathogenic fungi. The isolation method described represents a valuable tool for rapid and simple isolation of entomopathogenic fungi. These positive entomopathogenic fungi may have potential against variety pests in agriculture.

Isolation of Beauveria bassiana from the pine sawyer, Monochamus saltuarius Gebler and evaluation of its efficacy

Tae Young Shin,Jae Bang Choi,Sung Min Bae,Ye Rim Cha,Hyun Na Koo,Soo Dong Woo 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

Entomopathogenic fungi were isolated directly from a cadaver of adult M. saltuarius (infected with white fungi) supporting fungal sporulation, to develop biological control of pine wilt disease vector, M. saltuarius which was the most abundant in the middle to northern part of Korea and caused enormous damage to native pine tree in Korea, Japan and other regions of Asia. Pathogenicity of each fungus was tested using oak longicorn beetle, Moechotypa diphysis, as substitutive insect. As the result, only one of them showed pathogenic to adults of M. diphysis, with up to 100% mortality within 13 days of inoculation. Selected fungus was named as MsW1 and identified by Beauveria bassiana using microscopic examination, B. bassiana-specific PCR primers and genetic sequencing of the ITS region analysis. Pathogenicity test were conducted with various concentration of conidial suspensions of this isolate on M. saltuarius (3rd instar larvae and adults). Mortality rates varied from 57.1% to 100.0% and from 16.7% to 100.0% of M. saltuarius (3rd instar larvae and adults), respectively at 30 days. This is the first report of natural infection of M. saltuarius by B. bassiana.

Direct Monitoring of Membrane Fatty Acid Changes and Effects on the Isoleucine/Valine Pathways in an ndgR Deletion Mutant of Streptomyces coelicolor

Choi Tae-Rim,Oh Suk Jin,Hwang Jeong Hyeon,Kim Hyun Jin,Shin Nara,Yun Jeonghee,Lee Sang-Ho,Bhatia Shashi Kant,Yang Yung-Hun 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.6

NdgR, a global regulator in soil-dwelling and antibiotic-producing Streptomyces, is known to regulate branched-chain amino acid metabolism by binding to the upstream region of synthetic genes. However, its numerous and complex roles are not yet fully understood. To more fully reveal the function of NdgR, phospholipid fatty acid (PLFA) analysis with gas chromatography-mass spectrometry (GC-MS) was used to assess the effects of an ndgR deletion mutant of Streptomyces coelicolor. The deletion of ndgR was found to decrease the levels of isoleucine- and leucine-related fatty acids but increase those of valine-related fatty acids. Furthermore, the defects in leucine and isoleucine metabolism caused by the deletion impaired the growth of Streptomyces at low temperatures. Supplementation of leucine and isoleucine, however, could complement this defect under cold shock condition. NdgR was thus shown to be involved in the control of branched-chain amino acids and consequently affected the membrane fatty acid composition in Streptomyces. While isoleucine and valine could be synthesized by the same enzymes (IlvB/N, IlvC, IlvD, and IlvE), ndgR deletion did not affect them in the same way. This suggests that NdgR is involved in the upper isoleucine and valine pathways, or that its control over them differs in some respect.

Treatment with Bisphenol A Leads to the Promotion of Human Breast Cancer Cells and Alteration of Cell Cycle-Related Gene Expressions, cyclin E and p27

Kyung-Chul Choi, Hye-Rim Lee, Tae-Hee Kim 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.4

Endocrine disrupting chemicals (EDCs) have detrimental effects on human health. Among these EDCs, bisphenol A (BPA) binds to estrogen receptors (ERs) to stimulate estrogen-mediated responses. BPA is assumed to disrupt the reproductive and developmental system of humans. In addition, BPA has recently been suspected as a risk of carcinogenesis. Because BPA can cause abnormal estrogen-mediated response in the organism, exposure to BPA may stimulate growth of estrogen-dependent breast cancers in human. In breast cancer, cyclin E and cyclin-dependent kinase inhibitor p27 are important in G1/S phase transition during cell cycle progression. In this study, using an MTT assay, we investigated the effect of BPA on proliferation of MCF-7 breast cancer cells in vitro. In addition, we also analyzed the transcriptional levels of cyclin E and p27 following treatment with BPA using semi-quantitative RT-PCR. As a result, treatment with BPA resulted in significant induction of breast cancer cell growth, compared to a vehicle. BPA caused alterations of cyclin E and p27 mRNA expression. Expression of cyclin E was increased by BPA, while p27 was decreased at 24 h after treatment with BPA in MCF-7 breast cancer cells. Taken together, these collective results suggest that exposure to BPA induced breast cancer cell proliferation with deregulation of the cell cycle. A further study is required in order to determine the effects of BPA on the carcinogenic process in in vivo models.

주관절 부위의 다발생 결절종에 의한 척골신경 마비

최호림(Ho-Rim Choi),임영택(Young-Taeg Lim),최원태(Won-Tae Choi),이정웅(Jeong-Woung Lee) 대한정형외과학회 1999 대한정형외과학회지 Vol.34 No.4

Saucernetin-7 Isolated from Saururus chinensis Induces Caspase-Dependent Apoptosis in Human Promyelocytic Leukemia HL-60 Cells

CHOI, Seung-Ki,SEO, Bo-Rim,LEE, Kyung-Won,CHO, Woong,JEONG, Seoung-Hee,LEE, Kyung-Tae WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

In the present study, we investigated the effect of saucernctin-7 (a biologically active compound isolated from the underground parts of Saururus chinensi) on the indiiction of apoptosis and the putative pathways of its action in HL-60 human promyelocytic leukemia cells. Saucernetin-7-treated HL-60 cells displayed several features of apoptosis, including DNA fragmentation, DNA laddering by agarose gel electrophoresis, and externalization of annexin-V targeted phosphatidylserine (PS) residnes. z-VAD-fmk (a broad-caspase inhibitor) almost complctely suppressed saucernetin-7-induced DNA ladder formation, thereby implicating the caspase cascade in the apoptutic process. We also observed that saucemetin-7 caused the activations of caspase-3, -8 and -9, and that it induced Bid cleavage, the mitochondrial translucation of Bax from the cytosol, and cytochrome c release from mitochundria, but it had no effect on Bcl-2 and Bcl-xL levels. Taken together, the present study demonstrates that saucernetin-7 is a potent inducer of apoptosis and that its activity is facilitated by caspase-8 activation, Bid cleavage, Bax translocation to mitochondria, release of cylochrome c into cytuplasm, and subsequently rasuase-3 activation, which offers a potential mechanism for the apoptosis-inducing activity of saucernetin-7.

Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo

Choi Soyoung,Cha Jihye,Song Minji,Son JuHwan,Park Mi-Rim,Lim Yeong-jo,Kim Tae-Hun,Lee Kyung-Tai,Park Woncheoul 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.11

Objective: The study of Hanwoo (Korean native cattle) has mainly been focused on meat quality and productivity. Recently the field of microbiome research has increased dramatically. However, the information on the microbiome in Hanwoo is still insufficient, especially relationship between vagina and feces. Therefore, the purpose of this study is to examine the microbial community characteristics by analyzing the 16S rRNA sequencing data of Hanwoo vagina and feces, as well as to confirm the difference and correlation between vaginal and fecal microorganisms. As a result, the goal is to investigate if fecal microbiome can be used to predict vaginal microbiome. Methods: A total of 31 clinically healthy Hanwoo that delivered healthy calves more than once in Cheongju, South Korea were enrolled in this study. During the breeding season, we collected vaginal and fecal samples and sequenced the microbial 16S rRNA genes V3-V4 hypervariable regions from microbial DNA of samples. Results: The results revealed that the phylum-level microorganisms with the largest relative distribution were Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria in the vagina, and Firmicutes, Bacteroidetes, and Spirochaetes in the feces, respectively. In the analysis of alpha, beta diversity, and effect size measurements (LefSe), the results showed significant differences between the vaginal and fecal samples. We also identified the function of these differentially abundant microorganisms by functional annotation analyses. But there is no significant correlation between vaginal and fecal microbiome. Conclusion: There is a significant difference between vaginal and fecal microbiome, but no significant correlation. Therefore, it is difficult to interrelate vaginal microbiome as fecal microbiome in Hanwoo. In a further study, it will be necessary to identify the genetic relationship of the entire microorganism between vagina and feces through the whole metagenome sequencing analysis and meta-transcriptome analysis to figure out their relationship. Objective: The study of Hanwoo (Korean native cattle) has mainly been focused on meat quality and productivity. Recently the field of microbiome research has increased dramatically. However, the information on the microbiome in Hanwoo is still insufficient, especially relationship between vagina and feces. Therefore, the purpose of this study is to examine the microbial community characteristics by analyzing the 16S rRNA sequencing data of Hanwoo vagina and feces, as well as to confirm the difference and correlation between vaginal and fecal microorganisms. As a result, the goal is to investigate if fecal microbiome can be used to predict vaginal microbiome.Methods: A total of 31 clinically healthy Hanwoo that delivered healthy calves more than once in Cheongju, South Korea were enrolled in this study. During the breeding season, we collected vaginal and fecal samples and sequenced the microbial 16S rRNA genes V3-V4 hypervariable regions from microbial DNA of samples.Results: The results revealed that the phylum-level microorganisms with the largest relative distribution were <i>Firmicutes</i>, <i>Actinobacteria</i>, <i>Bacteroidetes</i>, and <i>Proteobacteria</i> in the vagina, and <i>Firmicutes</i>, <i>Bacteroidetes</i>, and <i>Spirochaetes</i> in the feces, respectively. In the analysis of alpha, beta diversity, and effect size measurements (LefSe), the results showed significant differences between the vaginal and fecal samples. We also identified the function of these differentially abundant microorganisms by functional annotation analyses. But there is no significant correlation between vaginal and fecal microbiome.Conclusion: There is a significant difference between vaginal and fecal microbiome, but no significant correlation. Therefore, it is difficult to interrelate vaginal microbiome as fecal microbiome in Hanwoo. In a further study, it will be necessary to identify the genetic relationship of the entire microorganism between vagina and feces through the whole metagenome sequencing analysis and meta-transcriptome analysis to figure out their relationship.

이식물에 따른 전방 십자 인대 재건술의 결과 비교 : 자가 골-슬개건-골, 동종 골-슬개건-골 그리고 동종 아킬레스건의 비교

최호림,최원태,이정웅,전재균,김의순,이장근,고염규 대한슬관절학회 2001 대한슬관절학회지 Vol.13 No.2

목적 : 전방 십자 인대 손상 환자를 대상으로 자가 및 동종 골-슬개건-골, 그리고 동종 아키레스 건을 이용해 관절경적 재건술을 시행하고 이들의 임상 경과를 분석하여 보고하고자 한다. 대상 및 방법 : 1995년 7월부터 2001년 4월까지 본원에서 전방 십자 인대 손상 환자를 대상으로 관절경적 재건술을 시행한 147례 중 2년 이상 추시가 가능했던 32명의 환자를 대상으로 하였다. 제 Ⅰ군(13 례)은 자가 골-슬개건-골 이식물을 이용하고, 제 Ⅱ군(11례)은 동종 아킬레스 건 이식술을, 그리고 제 Ⅲ군(8 례)에서는 동종 골-슬개건-골 이식술을 이용하겨 재건술을 시행하였다. 수술시 평균 환자의 나이는 34세였고, 평균 추시 기간은 35개월(25개월~54개월)이었다. 환자의 주관적 평가로 IKDV knee ligament standard evaluation form과 Lysholm knee scoring scale을 사용하였고 객관적 전·후방 안정도의 측정을 위해서 KT-2000TM arthrometer(MED metic, USA)를 사용하였다. Kruskal-Wallis test와 Mann-Whit-ney U(Wilcoxon Rank Sum) 검정법을 이용하여 세 집단을 비교 통계 분석하였다. 결과 : IKDC 최종 평가상 모든 군에서 B(거의 정상) 이상으로 양호하였고, 이식건 공여부 동통은 자가 골-슬개건-골을 이용한 군에서 2례 발생하였으나 통계학적인 유효성은 없었다. KT-2000^TM arthrometer에서는 30lb 부하시 정상측과 비교하여 슬 후 제 1군 1.96mm, 제 2군 2.32mm, 제 3군 2.6mm의 차이를 보여 만족스러운 결과를 보였다. Lysholm knee scoring scale을 이용한 평가는 제 Ⅰ군이 평균 88점(good), 제 Ⅱ군이 85점(goood), 제 Ⅲ군이 89점(good)이었다. 결론 : 전방 십자 인대 재건술시 이식건의 선택은 환자 개개인의 특성에 따라 달라져야 할것으로 생각되며, 동종 이식물도 임상적으로 좋은 결과를 보여 자가 이식건의 대체물로 사용될 수 있으리라 사료된다. Purpose : To compare the long-term results of reconstruction for the anterior cruciate ligament used by BPTB autograft, BPTB allograft and Achilles tendon allograft. Materials and Methods : We reviewed the results of 147 patients who had been managed with arthroscopic ACL reconstruction using different graft such as BPTB autograft, Achilles tendon allograft and allograft BPTB. Thirty two patients(average age, 34 years) were retrospectively evaluated. The follow-up period was more than two years(average, 35 months). Results were evaluated with IKDC knee ligament standard evaluation form, KT-2000^TM arthrometer, and Lysholm knee scoring scale. Kruskal-Wallis test and Mann-Whitney U test(Wilcoxon Rank Sun) were used for statistical analysis. Results : On IKDC scale, the final evaluation was nearly normal in all patients. We could not find statistical difference among the three groups by KT-2000TM arthrometer. Conclusion : The use of allografts may be an acceptable choice for ACL reconstruction.

Comparative bioavailability of synthetic vitamin C and Nutra-C (calcium ascorbate) in Korean healthy volunteers

Choi, Kyung-Mi,Hoon, Kim Man,Won, Hwang Tae,Kim, Jong-Dae,Park, Keum duck,Kim, Mi-Young,Jung, Young-Rim,Shin, Hye-Seoung The Korean Society of Analytical Science 2016 분석과학 Vol.29 No.4

The purpose of this study was to compare the relative bioavailability of synthetic Vitamin C and Nutra-C^® (calcium ascorbate) using a randomized parallel pharmacokinetics study design. Under fasting conditions, 20 healthy volunteers were randomly allocated to receive a single oral dose (500 mg of ascorbic acid) of either synthetic Vitamin C or Nutra-C^®. Fasting blood was collected pre-dose and 1, 2, 3, 4, 7 and 10 hr post-dose. The ascorbic acid content of human serum was determined using HPLC with ultraviolet detection. The fasting serum ascorbic acid concentrations of synthetic Vitamin C and Nutra-C^® were 6.734 ± 2.09 ng/mL (n = 10) and 7.542 ± 2.96 ng/mL (n = 10), respectively. The bioavailability of Nutra-C^® was significantly greater (128 %, p < 0.05) than that of the synthetic Vitamin C.