실험적 망상적혈구 증다증에 있어서 산화환원 효소활성의 변동 I : Ⅱ. Glucose 6-phosphate dehydrogenase

박주홍,이근배 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.15 No.1

토끼에 Phenylhydrazine을 주사한 후 시일이 경과하는 동안에 채혈한 혈액의 적혈구에서 glucose-6-phosphate dehydrogenase(D-glucose-6-phosphate : oxidoreductase, EC 1. 1. 1. 49)의 총 활성도의 변동과 세포질 및 mitochondria에 함유된 glucose-6-phosphate(G-6-P) dehydrogenase의 활성도의 변화를 관찰하여 다음과 같은 결과를 얻었다. G-6-P dehydrogenase의 활성도의 측정은 Komberg 및 Horecker의 방법에 따랐다. 1. 정상흰쥐 적혈구중의 망상적혈구의 수는 약 2.9%였다. 2. 정상 적혈구의 G-6_P dehydrogenase의 활성도는 약 3.8unit/㎖ RBC였으며 세포질 분획에는 약 99.0%가 mitochondria 분획에 약 1.0%가 분포되어 있었다. 3. Phenylhydrazine 주사후 5~6일이 경과하면 망상적혈구의 수는 최고치에 달하는 바 약 35배의 증가를 보였다. 이때 적혈구내의 G-6-P dehydrogenase의 총 활성도도 최고치에 달하는바, 정상치의 약 1.4배의 증가를 보였다. 총 활성도의 약 97.5%가 세포질 분획에, 약 2.5%가 mitochondria 분획에 증명되었다. 4. 망상적혈구증다증의 유발기에 있어서 G-6-P dehydrogenase의 총 활성도의 증가는 주로 세포질 분획내의 이 효소의 증가에 기인하였다.(Ks=0.0208, Kd=0.0042, t1/2=165.0 hours). 5. Mitochondria 분획내의 isozyme도 정상치의 최고 약 22.1배 증가하였다.(Ks=0.0013, Kd=0.0010, t1/2=69.3 hours). 6. 이 효소의 합성 및 분해속도 상수는 세포질 분획에 있어서는, Ks=0.0123, Kd=0.0464, t1/2=56.3 hours이며 mitochondria 분획에 있어서는, Ks=0.0003, Kd=0.0146, t1/2=47.5 hours 이었다. The activity of glucose 6-phosphate dehydrog-enase(D-glucose 6-phosphate : NADP 1-oxidoreductase, EC 1, 1, 1, 49) isozymes in rat red blood cells were studied during phenylhydrazine-induced reticulocytosis and recovery period from it. Glucose 6-phosphate dehydrogenase activity was assayed by the method of Kornberg and Horecker. The results obtained were as follows: 1. Red blood cells of normal rabbit contained about 2.9% of reticulocytes. 2. Total activity of G-6-P dehydrogenase in normal rat erythrocyte was 3.8 units and the relative activity of cytosolic and mitochondrial isozymes were 99.3% and 0.07% respectively. 3. The reticulocyte count in phenylhydrazine-treated rabbit increased to about 38 times on Day 4, Total G-6-P dehydrogenase activity reached maximal on Day 4, showing about 5.1 units which is about 1.3 fold higher than normal value. The increase in the total G-6-P dehydrogenase activity during induction of reticulocytosis was mostly due to the increase in the activity of cytosolic isozyme (Ks= 0.0208 : Kd=0.0042 ; t1/2=165.0 hours), and partly due to the increse in the activity of the mitochondrial isozyme (Ks=0.0013 ; Kd= 0.0100: t1/2=69.3 hours). 4. The rate constants following recovery from phenylhydrazine treatment were: Ks=0.04 64 : Kd=0.0123 ; t1/2=56.3 hours for cytosolic isozyme and Ks=0.0003 : Kd=0.0146 : t1/2=47.5 hours for mitochondrial isozyme.

움직임 추정 오차 분포를 이용한 H.263 부호화기의 진보 예측 모드의 동적선택

許泰元,李根泳 성균관대학교 1998 학술회의지원논문목록집 Vol.1998 No.-

본 논문은 H263 부호화기에서 진보 예측 모드(Advanced Prediction Mode, APM)를 동적으로 적용하여 계산량을 줄이고 부호화 효율을 높일 수 있는 APM의 동적 선택(Dynamic APM, DSPM)방법을 제안하였다. 화면내에 존재하는 움직임 성분을 분석하고, 그 성격에 따라 기본 예측모드(Default Prediction Mode,DPM)와 APM을 동적으로 적용할 수 있도록 하였다. 이를 위하여 매크로 블록 단위로 추정된 각 탐색점에서의 예측 오차로 구성된 움직임 추정 오차 분포(Error Distribution of Motion Estimation, EDME)를 구성하고, 이 분포를 네 개의 영역으로 분할한 다음, 각 분할 영역의 최소 예측 오차와 매크로 블록 단위로 추정된 예측 오차와의 비를 구하여, 블록 단위의 움직임 추정을 수행할 것인가를 결정하였다. 선형 움직임이 대부분인 화면에 대해서는 DPM으로 부호화 하도록 하여, 부분적으로 예측 효율이 떨어지는 것을 막을 수 있었으며, 고정된 APM(Fixed APM,FAPM)으로 부호화 하는 경우에 발생하는 계산량을 30%정도로 줄이면서, 평균적으로 으로 부호화 된 화질과 비슷한 결과를 얻을 수 있었다. In this paper, we proposed a dynamic selection scheme of advanced prediction mode(DAPM), whish reduces computational cost and improves coding efficiency. We can select the mode between default prediction mode(DPM) and advanced prediction mode(APM) accoding to motion components in a frame dynamically. For this purpose, we defined error distribution of motion estimation(EDME) as sum of absolute difference(SAD) for each searching points. This distribution region is divided to four subregions. We calculate minimu, values in each subregions and then, we determine whether block motion estimation is performed of not depending on the results. As a result, we reduced computational complexity to 30% without degradation of image quality compared to fixed APM(FAPM) by selecting DPM for linear movement.

실험적 망상적혈구 증다증에 있어서 산화환원 효소활성의 변동 : Ⅰ. Sorbitol dehydrogenas

허광덕,이근배 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.15 No.1

페닐히드라진을 정용한 토끼에 있어서 망상적혈구가 생성하였다가 소실되는 동안의 솔비톨 데히드로게나의 활성도 변화, 즉 이 효소의 세포질 및 미토콘드리아 분획내의 isozyme의 합성 및 분해속도를 측정하여 음과 같이 결론을 얻었다. 토끼의 정상 적혈구속에 솔비톨 데드로게나제의 활성을 확인하였다. 세포질내에는 적혈구 ㎖당 20.2 단위, 미토콘드라아 분획에는 적혈구 ㎖당 14.3단위의 활성도를 증명하였다. 세포질 분획속의 효성활성도는 페닐히드라진을 적용한지 5일후에 최고에 달하였으며 정상 토끼에 비하여 7.5배의 증가를 보여주었다.(K_(s)=2.7267;K_(d)=0.0184; t 1/2=37.9 hours). 미토콘프라아 분획속의 이 효소의 활성도는 페닐히드라진을 적용한지 역시 5일후에 최고에 달하였으며 상 토끼에 약 5배의 증가를 보여준다. 이 때의 활성도는 세포질 분획속의 활성도의 약 절반에 해당된다.(K_(s)=2.0520;K_(d)=0.0270; t 1/2=25.7hours.) 각 분획속의 이 효소 활성도의 증가 및 감소는 페닐히드라진으로 유발된 reticulocyte의 증가와 소멸해는 경과와 거의 일치한다. 이 현상을 세포분화에 따르는 에너지 요구 및 세포구성 성분의 형성에 관련시켜 비톨의 역할에 대하여 논의하였다. The rate of biosynthesis and degradation of sorbitol dehydrogenase isozymes of red blood cells from phenylhydrazine-treaed rabbits have been studied. The results obtaind were as follows: In normal red blood cells of rabbit, appreciable levels of sorbitol dehydrogenase activity were detected. The activity of sorbitol dehydrogenase in the cytosolic and mitochondrial fractions were increased significantly after phenylhydrazine treatment, and the maximal values were attained at the 5th day, which were about 7.5 times the initial value fot cytosotic isozyme and 5 time for mitochondrial isozyme, respectively. The pattern of time course changes in these two isozymes were quite similar each other. However, the levels of the mitochondrial isozyme was about one-half of that of the cytosolic isozyme. Our experimental data show that Ks value in the mitochondria of reticulocyte during the induction period of reticulocytosis was about 4.6-fold higher than that in the recovery period, while Kd in the induction period was almost equal to that in the recovery period. The increase in total sorbitol dehydrogenase activity during the induction of reticulocytosis appeared to be primarily due to the increase in the cytoslic isozyme (Ks=2.7267: Kd=0.0l84: t 1/2=37.9 hours) and partly due to the increase in the mitochondrial isozyme during reticulocytosis (Ks=2.0520: Kd=0.0270: t 1/2=25.7 hours). The rate constant dyring recovery period from phenylhydrazine treatment were : Ks=0.4311: Kd=0.03808: t 1 /2=22.5 hours for the mitochondrial isozyme and Ks=0.5820: Kd=0.0291: t 1 / 2=23.8 hours for the cytosolic isozyme. Possible role of sorbitol on the energy yielding and formation of cell components during red blood cell maturation was discussed.

공공서비스의 민간 위탁 성과에 영향을 미치는 요인

송근원,강대창,허남식 한국행정연구원 2003 韓國行政硏究 Vol.12 No.3

이 연구에서는 민간 위탁에 관한 이론적 배경과 경험적 사례를 통해서 공공서비스의 민간 위탁 성과에 영향을 미치는 여덟 개 요인들을 추출하여 유의한 영향을 미치는 변수가 무엇인가를 밝혀내고 이를 바탕으로 어떠한 여건 하에서 어느 요인들이 민간위탁의 성과에 유의한 영향을 미치는가를 알아보기 위해 군집분석 결과 제공된 자료들을 가지고 회귀분석을 실시하였다. 그 결과, 민간 위탁의 성공에 영향을 미치고 있는 유의한 요인들로는 전체적으로 볼 때 저항성, 측정가능성, 비용효율성, 서비스 중단시 파급효과라는 네 가지 조건들임을 찾아냈으며, 이 가운데 가장 많은 영향을 미치는 요인은 비용효율성이고, 그 다음이 저항성, 측정가능성, 서비스 중단시 파급효과의 순서이다. 한편, 이들 요인 이외의 경쟁성, 전문 기술적 복잡성, 기관장의 추진 의지, 행정지원체계의 적절성 요건 등은 공공서비스의 유형에 따라 민간 위탁 성과에 영향을 미치고 있음을 밝혀냈다. 이러한 분석 결과를 논의하면서 제시한 행정적 시사점을 요약하면 다음과 같다. 첫째, 민간 위탁 여건의 정확한 파악 없이 같은 종류의 공공서비스라는 이유로 민간 위탁을 무리하게 추진하는 것은 현실적합성이 결여될 수 있다. 따라서, 민간 위탁 여건에 따른 공공서비스의 분류 유형에 따라 민간 위탁의 성과에 영향을 미치는 요인들이 달라지는 까닭에 민간 위탁 이전에 여기에서 제시한 여덟 가지 민간 위탁 조건들을 점검함으로써 어느 유형에 속하는지를 판단하고 난 후 민간 위탁 여부를 결정할 것을 제안한다. 둘째, 기술적 전문성이 요청되는 공공서비스의 경우, 민간부문에 위탁하는 것이 좀더 적은 비용으로 양질의 공공서비스를 공급할 수 있다는 것을 시사한다. 셋째, 경쟁성이 낮다고 판단되는 공공서비스의 경우 민간 위탁을 통해 비용 절감을 추구하기 위해서는 적절한 성과 평가지표의 개발이 선행되어야 할 것을 주장한다. 넷째, 서비스 공급 중단으로 인한 사회적 파급효과가 큰 공공서비스의 경우, 신뢰할 만한 책임감 있는 수탁자를 경쟁적으로 선정한 후 적절히 지도, 감독하는 일이 매우 중요하다. 왜냐하면, 서비스 공급이 중단되는 경우, 서비스 공급을 다시 시작하려면 많은 시간과 노력 비용이 소요되고 여론이 악화되기 때문이다. 이 이외에도 위탁 조건 중 저항성은 민간 위탁의 성과에 가장 부정적인 영향을 미치는 요인으로 밝혀졌다. 따라서 조직내외의 저항성이 강하게 나타나는 공공서비스는 민간 위탁에 신중을 기할 필요가 있다. 곧 민간위탁을 무리하게 추진하기보다는 저항을 완화, 극복할 수 있는 제도적 방안을 마련한 후, 경험적 학습화를 거쳐 점진적으로 민간에 넘겨주는 방안을 적용해야 한다. constituents. Ultimately, the vision of Young Weol Gun was established as 'Pro-Environmental and Cultural Tourist and Relaxation City. The OECD countries have been practicing a series of government reform programs in order to cope with the pressures of the environmental change. These programs are basically aimed at enhancing public sector productivity and customer satisfaction through the adoption of result-oriented management system. The development of accounting system is an indispensable step toward a successful result-oriented management in government. Especially the cash-based governmental accounting system makes it hardly provide accurate information on the status and soundness of governmental financial management. Thus, many OECD countries are transforming the accounting system from cash accounting to accrual accounting. This study explores the causes, consequences, performance, and limitations of Australian governmental accounting system reform for the successful adoption of the new accounting system in Korea. This study shows the successful Australian experience with its decentralization and strengthened accountability of the public bureaucracy, and the new system provides valuable information for better management decisions. Continuous efforts based on active participation of private and public sector, an official's will and capability to apply new information can play an important role in the system. But to reform the governmental accounting system successfully, it needs to clarify the limitation of financial information that new system products. And finally, under this circumstances, this study implies that supplemental measures like budget system reform and performance management system of measuring policy effects should be accompanied. This paper investigates the factors which have influenced on the local public services contracting-out outcomes. This study aims to provide the theoretical grounds of the factors improving local public services. Based not only on the theoretical backgrounds and empirical case study, but also on the interviewing results with public servants who deal with contracting-out, eight criteria are selected. With this conditions cluster analysis is done for the public services and the groups newly classified are discussed in terms of their characteristics. The variables influencing these newly classified five groups of the services are examined by multiple regression analysis. The research findings suggest as follows: The success in contracting-out is highly dependent on the higher cost-efficiency, on the lower resistance, on the higher measurability of outcomes, and on the higher spill-over effect in discontinued services when the services are stopped to be supplied with. Alongside introducing "research councils" in public R&D governance system, the Korean government has carried out the evaluation of member research institutes under research councils every year since 2000. However, the current evaluation system strongly needs to be improved, mainly due to both some deficits of system itself and unfavourable effects arose from the actual implementation process. Therefore, this paper aims to suggest some policy implications for the development of the Korean institute evaluation system, taking the Institute Assesment Exercise (IAE) of the Biotechnology and Biological Sciences Research Council (BBSRC) in the UK as a bench-marking model. As a result, the IAE practice of the BBSRC will be a good reference when three Korean research councils in S&T sector intend to develop their current institute evaluation system. In particular, the UK case will provide useful policy implications for the improvement of some limitations of the Korean system, including the focus of managerial performance, one year interval between evaluation exercises, lower expertise in both evaluation manpower and evaluation unit, the adoption of ranking system among the institutes and budget adjustment-focused utilisation, which have been regarded as main problems in the Korean case.

작물의 음향 신호에 대한 반응 분석에 관한 연구

이근영,허태원,김응성,이재원 成均館大學校 科學技術硏究所 1996 論文集 Vol.47 No.2

The puper studies the relationship between plants and audio signals. Responses of plant are transformed to electrical signals and digitized to process them on computer. Variance and mean are used for spatial domain analysis. and Fourier transform is used for frequency responses analysis as tools. We used celery cabbage, cucumber, parsley and onions as experimental plants. With this experiments, we found out that specific plants are sensitive to specific music signals and external stimulus.

[CF-0002] Transcriptional analysis of differentially expressed genes related with disease resistance in Vitis species for grape breeding

Hae Keun Yun(Hae Keun Yun),Soon Young Ahn(Soon Young Ahn),Hae In Lee(Hae In Lee),Youn Young Hur(Youn Young Hur) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Inhibitory Effect of Bee Venom Toxin on Lung Cancer NCI H460 Cells Growth Through Induction of Apoptosis via Death Receptor Expressions

Hur, Keun Young,Song, Ho Sueb Korean AcupunctureMoxibustion Medicine Society 2014 대한침구의학회지 Vol.25 No.1

Objectives : I investigated whether bee venom inhibit cell growth through enhancement of death receptor expressions in the human lung cancer cells, NCI-H460. Methods : Bee venom(1-5 ${\mu}g/ml$) inhibited the growth of NCI-H460 lung cancer cells by the induction of apoptotic cell death in a dose dependent manner. Results : Consistent with apoptotic cell death, expression of TNF-R1, TNF-R2, FAS, death receptors(DR) 3, 4, 5 and 6 was increased in the cells. Expression of DR downstream pro-apoptotic proteins including Caspase-8, -3, -9 was upregulated and Bax was concomitantly overwhelmed the expression of Bcl-2. NF-kB were inhibited by treatment with bee venom in NCI-H460 cells through TNF response change led by TNF-R1 and TNF-R2. Conclusions : These results suggest that bee venom should exert anti-tumor effect through induction of apoptotic cell death in NCI-H460 human lung cancer cells via enhancement of death receptor expression, and that bee venom could be a promising agent for preventing and treating lung cancer.

Potential Implications of Long Noncoding RNAs in Autoimmune Diseases

Keun Hur,Sang-Hyon Kim,Ji-Min Kim 대한면역학회 2019 Immune Network Vol.19 No.1

Inhibitory Effect of Bee Venom Toxin on Lung Cancer NCI H460 Cells Growth Through Induction of Apoptosis via Death Receptor Expressions

Keun Young Hur,송호섭 대한침구의학회 2014 대한침구의학회지 Vol.31 No.1

Objectives : I investigated whether bee venom inhibit cell growth through enhancement of death receptor expressions in the human lung cancer cells, NCI-H460. Methods : Bee venom(1-5 μg/ml) inhibited the growth of NCI-H460 lung cancer cells by the induction of apoptotic cell death in a dose dependent manner. Results : Consistent with apoptotic cell death, expression of TNF-R1, TNF-R2, FAS, death receptors(DR) 3, 4, 5 and 6 was increased in the cells. Expression of DR downstream pro-apoptotic proteins including Caspase-8, -3, -9 was upregulated and Bax was concomitantly overwhelmed the expression of Bcl-2. NF-kB were inhibited by treatment with bee venom in NCI-H460 cells through TNF response change led by TNF-R1 and TNF-R2. Conclusions : These results suggest that bee venom should exert anti-tumor effect through induction of apoptotic cell death in NCI-H460 human lung cancer cells via enhancement of death receptor expression, and that bee venom could be a promising agent for preventing and treating lung cancer.

Different Impact of Diabetes Mellitus on In-hospital and 1-Year Mortality in Patients with Acute Myocardial Infarction Who Underwent Successful Percutaneous Coronary Intervention: Results from the Korean Acute Myocardial Infarction Registry

( Keun Ho Park ),( Young Keun Ahn ),( Myung Ho Jeong ),( Shung Chull Chae ),( Seung Ho Hur ),( Young Jo Kim ),( In Whan Seong ),( Jei Keon Chae ),( Taek Jong Hong ),( Myeong Chan Cho ),( Jang Ho Bae ) 대한내과학회 2012 The Korean Journal of Internal Medicine Vol.27 No.2

Background/Aims: The aim of this study was to evaluate the impact of diabetes mellitus (DM) on in-hospital and 1-year mortality in patients who suffered acute myocardial infarction (AMI) and underwent successful percutaneous coronary intervention (PCI). Methods: Among 5,074 consecutive patients from the Korea AMI Registry with successful revascularization between November 2005 and June 2007, 1,412 patients had a history of DM. Results: The DM group had a higher mean age prevalence of history of hypertension, dyslipidemia, ischemic heart disease, high Killip class, and diagnoses as non-ST elevation MI than the non-DM group. Left ventricular ejection fraction (LVEF) and creatinine clearance were lower in the DM group, which also had a significantly higher incidence of in-hospital and 1-year mortality of hospital survivors (4.6% vs. 2.8%, p = 0.002; 5.0% vs. 2.5%, p < 0.001). A multivariate analysis revealed that independent predictors of in-hospital mortality were Killip class IV or III at admission, use of angiotensin converting enzyme inhibitors or angiotensin-II receptor blockers, LVEF, creatinine clearance, and a diagnosis of ST-elevated MI but not DM. However, a multivariate Cox regression analysis showed that DM was an independent predictor of 1-year mortality (hazard ratio, 1.504; 95% confidence interval, 1.032 to 2.191). Conclusions: DM has a higher association with 1-year mortality than in-hospital mortality in patients with AMI who underwent successful PCI. Therefore, even when patients with AMI and DM undergo successful PCI, they may require further intensive treatment and continuous attention.