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표고버섯의 건조조건에 따른 GMP와 Lenthionine함량의 변화
최춘순,장인애,조덕봉 광주보건대학 1981 論文集 Vol.6 No.-
For the dried shiitake mushroom (Lentinus edodes), its two characteristic components GMP (guanosine mono phosphate) and Lenthionine contents were analysed through the HPLC and sublimation apparatus, and their results are studied with the effect of dehydration temperature and air velocity. In the commonly used temperature range 45∼50℃, GMP contents were increased with the temperature increase, and above the range 50℃, showed flattened curve, but the lower temperature 40℃ revealed high content. GMP contents were showed sharp increase for the range 1.5m/see to 2.0m/sec, but above the range showed the small difference. Lenthionine contents were decreased in relation to the temperature increase, and their decreasing trends showed more sharply with the air velocity increase 1.5m./sec to 2.0m./sec. From those results, the better qualified shiitake mushroom represented as GMP and Lenthionine content were obtained near the dehydration temperature 40℃. Whence, in case of commonly used dehydration temperature 45∼50℃, other controlled operations should be required, which could obtain the mushroom inner temperature 40℃, such as humidity control. Optimum air velocity could be selected as 2m/sec, which have made possible high content GMP and coincide with reasonable dehydration rate.
소수성 물질을 포함한 커들란 겔의 특성 및 식품으로의 활용 연구
최춘순,이기영,이창문,정제명,이인영 전남대학교 촉매연구소 2001 觸媒硏究 論文集 Vol.22 No.-
The thermal stability of curdlan suspension was investigated. Also curdlan gel containing hydrophobic materials (DHA(Docosa hexaenoic acid), olive oil and Dong-chunghacho (Militaris)) were prepared. Curdlan suspension prepared under 40℃ and 15000rpm was stable for 1 hour. Curdlan gel, including hydrophobic material were dried at 30℃ and swollen in the water. Curdlan gel including Dongchunghacho absorbed water of 980%.
최춘순 광주보건대학 1989 論文集 Vol.14 No.-
Volumetric oxygen transfer rate coefficient is important as an index for the oxygen supply. And it is influenced by the several operating conditions ; size and shape of impellers, aeration rate, rotational speed of impeller and rheological properties of fermentation broth such as viscosity. Most of the fermentation broth, viscosity will be changed in the 'progress of cultivation of microorganism and production of polymer such as dextran and pullulan. Oxygen transfer for the highly viscous solution could be achieved sufficiently- by the proper K_(L)a values. In this thesis, to study the viscosity effect on volumetric oxygen transfer rate coefficient, K_(L)a, sucrose solution and xanthan solution were employed., And then oxygen concentration was measured by the D.O. probe and its amplified reader. The experimental results were analyzed by the Dynamic Method, which were resulted K_(L)a values. Determined K_(L)a values for each solution system of viscosity were analyzed by the mathematical modeling and least square fit techniques. The equations for the K_(L)a and viscosity were derived as follows. For the sucrose solution flat blade impeller K_(L)a=85.8μ^(-0.95) axial flow turbine impeller K_(L)a=64.1μ^(-0.76) marine propeller type impeller K_(L)a= 61.0μ^(-0.81) For the xanthan solution flat black impeller K_(L)a=321μ^(0.50) axial flow turbine impeller K_(L)a=222μ^(-0.45) marine propeller type impeller K_(L)a=189μ^(-0.42) Those mathematical models were simulated for the each viscosity values of the solutions. And so, the simulated results were compared to the experimental results. Comparison of the experimental results and calculated results showed good agreements. And thus those mathematical models presented as appropriate.
Separation of Soybean Trypsin Inhibitor Using Reverse Micellar System
조상우,최춘순,이준식,Jo, Sang-Woo,Choi, Choon-Soon,Lee, Jun-Sik The Korean Society for Biotechnology and Bioengine 1989 KSBB Journal Vol.4 No.2
역미셀계를 이용하여 대두 트립신 저해제의 분리에 대하여 고찰한 바, 여러 완충 수용액 중에서 1.0M $C_aCl_2용액(pH3.0)과 1.0M NaCl용액(pH 11.5)이 단백질의 용해와 회수에 가장 적당하였다. 이 조건을 두 모델계와 하나의 실제시료에 적용해 본 결과, 7S 단백질과 트립신저해제로 구성된 모델계에서는 비활성이 두배이상 증가되었고, 선수용성 단백질과 트립신 저해제로 구성된 모델계에서는 1.6배의 비활성 증가를 볼 수 있었다. 광교대두로부터 추출한 전유출물의 경우, 1.56배의 비활성 증가가 있었다. 이들을 SDS-PAGE로 확인한 결과 모두 매우 순수한 단백질임을 알 수 있었다. The separation of trypsin inhibitir using reverse micellar system was invertigated. Among the biffer system tested, 1.0M $CaCl_2$ solution (pH 3.0) and 1.0M NaCl soluation (pH 11.5) were most effective for solubilization and de-solubilization of protein, respectively. When these conditions were applied to two model sampeles, one of which was composed of the same amount of 7S protein and trpsin inhibitor, and the other of which was composed of the same amount of soluble soybean protein isolates and trypsin inhibitor, highly pure trypsin inhibitor was obtained. And in the real soybean, Kwng Gyo, pure trypsin inhibior was also obtained.