Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

도로교통소음의 수평·수직적 전달 및 감쇠 특성

최형일,채희정,김연풍,조도호,설동화,정숙경,김정애 조선대학교 생산기술연구소 2007 生産技術硏究 Vol.29 No.1

This study has been conducted to achieve the following objectives: First, in order to understand the horizontal propagation and attenuation characteristics for the road traffic noise, we selected actively developed areas within 100 meters away from the road to measure the level of traffic noise near a road. To measure the noise near a railroad, we selected areas within 100 meters away from the road. In this way, we analyzed the horizontal propagation and attenuation characteristics for the traffic noise occurring in diversified areas. Second, in order to understand the vertical propagation and attenuation characteristics for the road traffic noise, we measured and analyzed the distributional characteristics of vertical sound pressure levels on each floor of multi-storied apartment buildings according to changes of traffic load and types, and the existence or nonexistence of soundproof walls. For the case of the road traffic noise, we also selected we measured and analyzed the different noise levels on each floor of multi-storied apartment buildings from the soundproof wall. The results of Horizontal propagation and attenuation characteristics for the road traffic noise are as follows: In cases of the soundproof wall + raising ground, raising ground, cutting land, and flat land as distance increases, the sound pressure level steadily decreases. The soundproof wall + raising ground has an especially high distance decrease compared to the other areas, and the sound pressure level for the raising ground is higher than that of the cutting land with a measurement of 10.7~12.1dB(A). Vertical propagation and attenuation characteristics for the road traffic noise are as follows: The amount of sound pressure level decrease is 12.6- 14.5dB(A) for first floor when measuring the vertical sound pressure levels at soundproof wall, which indicates a fairly large decrease. At fifth and sixth floor, the amount of decrease is 2.3dB(A), which indicates a small decrease.

타이타늄의 주조성

최기열,장경수,윤숙진 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.2

In order to investigate the castability of titanium, Kennedy Class 1, division 2 removable partial denture frameworks were designed. According to the manufacturer's instruction, 10 casting bodies each were obtained with pure titanium and cobalt-chrome alloy. The results were as follows ; 1. Castability of titanium was comparable with that or cobalt-chrome alloy in terms of fitness and surface detail reproducibility. 2. There was no radiographically detectable porosity in each casting body of titanium and cobalt-chrome alloy. 3. The number of internal porosities and narrow, long defect were almost same between titanium and cobalt-chrome alloy. 4. Vickers hardness number of cobalt chrome alloy was 439.4±16.1 and that of titanium was 200.1±23.3. 5. Biocompatibility and low density of castable titanium would provide denture rearers with multiple benefits.

담양신설 IC의 교통소음도 조사연구

최형일,강영주,채희정,김연풍,조도호,설동화,정숙경 조선대학교 환경공해연구소 2007 環境公害硏究 Vol.20 No.1

After the vehicle has become a large formation with high-speed, loud noise from an express highway is increasing, and the number of large-scale apartment complexes, which are recently being constructed in the metropolitan area nearby the express highway and IC, is increasing gradually. Compared with a main road, the traffic volume and the speed of a vehicle between IC and the ramp way [turn-off] are smaller. If the ramp way is rightly connected with the downtown area, however, the traffic-noise that comes about in surrounding areas will be in conjunction with the traffic-noise from the main road of express highway and that of the ramp way. This research has selected a place that hasn't had a road or a commercial quarter nearby the main-road of IC and ramp way. Then we have studied the traffic-noise status under minimizing influence of its outer-noise, which occurs in IC. 1. The traffic volume change of a small and full-sized car with time has been kept on regularity in 2004 and 2005. As the traffic volume of a full-sized car has diminished remarkably, it has been maintaining through 6:00a.m.-10:00 a.m. As it has decreased since lO:OO a.m.. we have also observed the traffic proportion going up. 2. Monthly traffic volume of small cars was higher in May, August and October than other months. We have observed that the high traffic volume during these months is due to the following: May-The Damyang festival, August-a high-demand season, October-viewing scarlet maple leaves. Monthly traffic volume of full-sized cars was generally higher in August, but it has decreased during 2006. This was due to the many construction trucks that were working on the extension of the 88 Olympic expressway. Therefore, there was more traffic volume in 2006 compared to 2004 and 2005. 3. In this study, the noise figures have been less than 55 dB(A) in 18 spots. There were 14 spots from 55dB(A) to 58dB(A). 58-61dB(A) : 20, 61-64dB(A) : 17, 64-67dB(A) : 18. There were 8 spots we observed at the highest level from 67dB(A) to 70dB(A). Those were the nearby spots separating the main road and Damyang IC. 4. Traffic-noise at the boundary between IC and the main road of an expressway was higher than that of the same distance in other places. 5. We have observed that traffic-noise is low in places with mountains that serve the role of soundproofing walls. Thus, the fundamental sound hadn't affected it.

正常 및 腫瘍性骨組織의 Lectin結合에 關한 免疫酵素組織化學的硏究

尹錫雄,崔宗相,白承龍 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1

To Clarify the patterns of lectin binding on the normal bone and cartilage and the changes in lectin receptors between the normal and neoplastic bone tissues, the author had performed an immunoenzyrne histochemical study. Eight lectins such as Concanavalin-A(Con-A). Dolichos biflorus agglutinin(DBA), peanut agglutinin(PNA), Ulex europaeus agglutinin I(UEA I), soybean agglutinin(SBA), wheat germ agglutinin(WGA), Ricinus communis agglutinin I (RCA I), and Pinellia ternata agglutinin(PTA) were used oil the normal bone arid cartilage tissue in addition to each three cases of osteoblastoma, osteogenic sarcoma, enchondrorna, chondrosarcorna, giant cell tumor, Ewing's tumor, multiple myeloma, simple bone cyst, and fibrous dysplasia. Avidinbiotin system was used and substrate used was 3-3' diaminobenzidine. The results obtained are as follows; 1. In the normal and neoplastic bone tissue, PKA, Con-A, PTA, RCA I, and WGA showed positive reactions in the cystoplasms of the osteocytes and osteoblasts. 2. In the normal and r,eoplastic chondroid tissues, RCA I showed positive reactions in the chondrocytes. 3. On the neoplastic cells of the Ewing's tumor, DBA, UEA I, Con-A and PTA showed cytoplasmic positive reactions, and on the plasma cells of the multiple myeloma, PNA, Con-A, PTA, RC-4 I, and WGA showed positive reactions. 4. On the neoplastic cells of the giant cell tumor of bone, Con-A, PTA, and WGA showed positive reactions, contrary to negative reactions of the above 8 lectins to the giant cells of the tumor. 5. PNA, Con-A PTA, and RCA I showed positive reactions against the fibrous spindle cells of the simlpe bone cyst and Con-A, PTA, and RCA I showed-positive reactions of the spindle cells of the fibrous dysplasia. With the above results, the author can find that there is no difference of lectin receptor between the normal and neoplastic bone and chondroid tissue and also confirm the diffenerence of lectin binding pattern between the osteoclasts and giant cells of the giant tumor.

다소성 골괴사

서유성,박형근,최형석,김연일,이병일 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.2

In case of the occurrence of osteonecrosis at three or more anatomical location, it is defined as multifocal osteonecrosis, but still any king of study with references hasn't been reported in Korea. The patient who had admitted due to the pain in both knee joints, both hip joints and left ankle joint and we diagnosed as multifocal osteonecrosis by using bone scan and magnetic resonance imaging. To relieve right knee joint and left hip joint pain, we perfomed arthroplasty. Here we present one case report of multifocal osteonecrosis through analysis by patient's history and hematologic examination and provide a review of the literature.

한국성인들의 사회경제적 요인 및 구강보건의식행태에 따른 치아우식증 실태분석

송근배,최연희,홍석진,김진범 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

The aim of this study was to analyze the association of the dxperience of dental caries with socioeconomic status and dental health behaviors among Korean adults as one of the results of National Oral Health Survey(NOHS) data in 2000. A total of 6,736 was collected from 7,027 households which were visited and interviewedfrom June 30th, 2000 to NOHS 28소, 2001 in NOHS. The subjects were more than 20 years old (men 2,754, women 3,982). The results of this study were obtained as below; 1. An average of DMFT index in women(7.20) was considerably higher than in men(4.90). The DMFT index of the people in twenties(4.53) and aged more than 70 was 4.53 and 14.28 respectively. The number of caries experienced teeth increased graduaily as the subjects were getting older. 2. There was negative relationship between caries experience and dental education levels, as opposed to monthly household income as a indicator of economic status. 3. In lihgt of caries experienced teeth connected to subjective recognition of oral health status and concern about oral health, it showed that Korean people have already known about their oral health status with concern. 4. It would be recommended that some specified measures such as index of dental root caries prevalence should be added and that the questionnaire should be improved for the second NOHS in 2003.

복수과 진료 환자의 중복 처방에 대한 분석 및 평가

이지은,장혜경,오지영,유윤경,김현지,임숙인,연숙희,강진숙,최귀령 한국병원약사회 2003 병원약사회지 Vol.20 No.1

In an aging society, there is an increasing possibility of the duplication of the drugs given to patients because they take many kinds of drugs. Among the prescriptions given to the patients who was treated at multiple ambulatory clinics in St. Mary's hospital for one month of September, 2002, in which drugs that has identical or similar effects are prescribed, we analysed and evaluated them by patients' characteristics, kinds of medication, severity of side effects on a case by case basis. More, we assessed the potential additional costs. As a result of this study, the duplication rate turned out to be 6.69% and it was shown that the proportion of the elderly patients over 60 was high. The gastro-intestinal medications took up a large part and most of the cases showed that the danger caused by double taking of medicine was slight, but there were some examples in which the serious side effects were predicted. Expected additional costs were the average 11.349 won and it ranges from the minimum of 56 won to the maximum of 135,720 won. In conclusion, the management of drug histories of the patients who need the plural treatments is very important and the necessity of the individual and professional guidance of taking medicines for the elderly patients is emerged, too.

C1q/TNF-Related Protein-3 (CTRP-3) and Pigment Epithelium-Derived Factor (PEDF) Concentrations in Patients With Type 2 Diabetes and Metabolic Syndrome

Choi, Kyung Mook,Hwang, Soon Young,Hong, Ho Cheol,Yang, Sae Jeong,Choi, Hae Yoon,Yoo, Hye Jin,Lee, Kwan Woo,Nam, Moon Suk,Park, Yong Soo,Woo, Jeong Taek,Kim, Young Seol,Choi, Dong Seop,Youn, Byung-Soo American Diabetes Association 2012 Diabetes Vol.61 No.11

<P><B/></P><P>Recent studies have suggested that a novel adipokine, C1q/tumor necrosis factor-related protein-3 (CTRP-3), a paralog of adiponectin, may play an important role in the regulation of glucose metabolism and innate immunity. Pigment epithelium-derived factor (PEDF), a multifunctional protein with antioxidant and anti-inflammatory properties, is associated with insulin resistance and metabolic syndrome. We examined circulating CTRP-3 and PEDF concentrations in 345 subjects with diverse glucose tolerance statuses. Furthermore, we evaluated the involvement of CTRP-3 and PEDF with cardiometabolic risk factors including insulin resistance, high-sensitivity C-reactive protein (hsCRP), estimated glomerular filtration rate (eGFR), and brachial-ankle pulse wave velocity (baPWV). CTRP-3 concentrations were significantly higher in patients with type 2 diabetes or prediabetes than the normal glucose tolerance group, whereas PEDF levels were not different. Subjects with metabolic syndrome showed significantly higher levels of both CTRP-3 and PEDF compared with subjects without metabolic syndrome. Both CTRP-3 and PEDF were significantly associated with cardiometabolic parameters, including waist-to-hip ratio, triglycerides, HDL-cholesterol, alanine aminotransferase, eGFR, hsCRP, and baPWV. In conclusion, circulating CTRP-3 concentrations were elevated in patients with glucose metabolism dysregulation. Both CTRP-3 and PEDF concentrations were increased in subjects with metabolic syndrome and associated with various cardiometabolic risk factors.</P>

Comparison of the Antimicrobial and Sanitizer Resistance ofSalmonellaIsolates from Chicken Slaughter Processes in Korea : <i>Salmonella</i>in chicken slaughterhouses…

Youn, So Youn,Jeong, Ok Mi,Choi, Byung Kook,Jung, Suk Chan,Kang, Min Su Wiley Blackwell (Blackwell Publishing) 2017 Journal of Food Science Vol.82 No.3