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      • 한국산 하루살이목(目)의 카탈로그

        윤일병,배연재,전동준 고려대학교 한국곤충연구소 1994 昆蟲硏究誌 Vol.20 No.1

        韓國産 하루살이目의 카탈로그. 裵淵宰 · 尹一炳^1 · 田東埈^2 (서울女子大學校, ^1高麗大學校 生物學科, ^2러시아科學院 動物學硏究所) 昆蟲硏究誌, 20: 31~50, 1994. 韓國産 하루살이目의 關한 硏究는 南北韓에 걸친 綜合的인 硏究가 이루어지지 못하였고, 1940년 以來 各種 文獻에 記錄된 種들에 있어서도 分類上 혹은 命名上 變動이 많았으나 이를 體系的으로 收錄하지 못하였다. 이 論文에서는 種合的인 文獻 및 模式標本 調査를 통하여 다음과 같이 韓國産으로 記錄된 56種의 命名상 有效한 種들에 대한 카탈로그를 작성하였다. Acerella longicaudata(U′eno), *Ameletus costalis (Matsumura), A. montanus Imanishi, *Baetiella japonica (Imanishi), *B, tuberculata (Kazlauskas), *Baetis thermicus U′eno, Bleptus fasciatus Eation, Choroterpes altioculus Kluge, Cincticostella castanea (Allen), C. tshernovae(Bajkova), * Cinygmula grandifaliz Tshernova, *C. hirasana (Imanisht), Cloeon dipterum (Linnaeus), Drunella aculea (Allen), *D. cryptomeria (Imanishi), *D. lepnevae (Tshernova), D. triacantha (Tsher-nova), *D. trispina ezoensis (Gose), Ecdyonurus bajrovae Kluge, E. dracon Kluge, **E. Joernensis mon-golicus Bajkova & Varychanova, E. kibunensis Imanishi, E. levis (Nava′s), E. scalaris Kluge, **E. subspinosa (Braasch & Solda′n), Epeorus curvatulus Matsumura, *E. hiemalis Imanishi, E. latifolium Ue′no, E. pellucidus (Broadsky), *Ephemeralla aurivillii Bengtsson, *E. imanishii Gose, E. keijoensis Allen, *E. notofascia Yoon & Base, Ephemera orientalis McLachlan, E. separigata Bae, E. strigata Eaton, Ephoron shigae (Takahashi), Heptagenia guranica Belov, H. kihada Matsumura, *H. Kyotoensis Gose, *Iron aesculus (Imanishi), *I. koreanicus Braasch & Solda′n, I. maculatus Tshernova, Isonychia japonica (Ulmer), **I. ussurica Bajkova, Paraleptophlebia chocolata Imanishi, *Potamanthellus rarus tiunova & Levanidova, **Potamanthus formosus Eaton, P. yooni Bae & McCafferty, *P. luteus oriens Bae & McCafferty, *Phithrogena japonica Ue′no, Rhoenanthus coreanus (Yoon & Bae), *Serratella setigera (Bajkova), Siphlonurus chankae Tshernova, *S, sanukensis Takahashi 및 Uracanthella rufa (Imanishi). 별표한 種들은 幼蟲만이, 쌍별표한 種들은 成蟲만이 韓國産으로 記載되었다. (C. tshernovae와 E. keijoensis는 암컷 成蟲과 幼蟲만이, 그리고 P. luteus oriens는 亞成蟲만이 韓國産으로 記載되었다.) 다음 10종은 韓國에서 幼蟲만이 記載되었고, 아직 命名上으로 留保된 未決定種으로 남아있다. Baetis Kua: Yoon & Bae, Baetis nla: Imanishi, Brachycercus Kua: Yoon & Bae, Caenis Kua: Yoon & Bae, Cinygmula Kua: Yoon & Bae, Cinygmula na: Imanishi, Ecdyonurus Kub: Yoon & Bae, Ecdyonurus na: Imanishi, Paraleptophlebia nb: Imanishi 및 Rhithrogena na: Imanishi. 각 種에 있어서 原記載 및 再記載 文獻, 記載에 使用된 標本, 分布, 模式標本에 대한 情報, 異名과 命名上의 變動을 年代順으로 整理하였다. 附錄으로 南韓種만의 checklist와 分類學的 變動 및 國名의 變動事項을 表로 作成하였다. Mayflies (Ephemeroptera) of Korea have been described in various literatures since 1940, but there have been many taxonomic or nomenciatural changes in the members of the order. A comprehensive literature and type material review of the Ephemeroptera resulted herein the recognition of 56 nomenclaturally valid species previously described from Korea : Acerella longicaudata (Ue´no), ^*A meletus costalis (Matsumura), A. montanus Imanishi, ^*Baetis thermicus Ue´no, Bleptus fasciatus Eaton, Choroterpes altioculus Kluge, Cincticostella castanea (Allen), C. tshernovae (Bajkova), ^*Cinygmula grandifolia Tshernova, ^*C. hirasana (Imanishi), Cloeon dipterum (Linnaeus), Dyunella aculea (Allen), ^*D. cryptomeria (Imanishi), ^*D. tepnevae (Tshernova), D. triacantha (Tshernova), ^*D. trispina ezoensis (Gose), Ecdynurus bajrovae Kluge, E. dracon Kluge, ^*^*E. joernensis mongolicus Bajkova & Varychanova, E. kibunensis Imanishi, E. levis (Nava´s), E. scalaris Kluge, ^*^*E. subspinosa (Braasch & Solda´n), Epeorus curvatulus Matsumura, ^*E. hiemalis Imanishi, E. latifolium Ue´no, E. notofascia Yoon & Bae, Ephemera orientalis McLachlan, E. separigata Bae, E. strigata Eaton, Ephoron shigae (Takahashi), Heptagenia guranica Belov, H. kihada Matsurnura, ^*H. kyotoensis Gose, ^*Iron aesculus (Imanishi), ^*I. Koreanicus Braaich & Solda´n, I. Maculates Tshernova, Isonychia japonica (Ulmer), ^*^*I. Ussurica Bajkova, Paraleptophlebia chocolate Imanishi, ^*Potamanthellus rarus Tiunova & Levanidova, ^*^*Potamanthus formosus Eaton, P. yooni Bae & McCafferty, ^*^*P. luteus oriens Bae & MeCafferty, ^*Rhithrogena japonica Ue´no, Rhoenanthus coreanus (Yoon & Bae), ^*Serratella setigera (Bajkova), Siphlonurus chankae Tshernova, ^*S. sanukensis Takahashi, and Uracanthella rufa (Imanishi), The asterisked species were known to larva only from Korea, and the double asterisked were adults only (C. tshernovae and E. keijoensis were females and larvae only, and P. luteus oriens was subimagos only), The following 10 species were known to larvae only from Korea, but have not been determined: Baetis Kua: Yoon & Bae, Cinygmula Kua: Yoon & Bae, Baetis nla: Imanishi, Brachycercus Kua: Yoon & Bae, Caenis Kua: Yoon & Bae, Cinygmula Kua: Yoon & Bae, Cinygmula na: Imanishi, Ecdyonurus Kub: Yoon & Bae, Ecdyonurus na: Imanishi, Paraleptophlebia nb: Imanishi, and Rhithrogena na: Imanishi. For each species, original and secondary sources of the descriptions, materials used, type information, distributions, synonyms, aid nomenclatural changes were chronologically provided. A checklist of the Ephemeroptera previously recorded from South Korea was appended with their proposed Korean name.

      • KCI우수등재

        재래한우의 보존을 위한 혈청 및 혈구단백질의 유전적 다형현상

        한상기(S . K . Han),윤희섭(H . S . Yoon),정의룡(E . Y . Chung),신유철(Y . C . Shin),변희대(H . D . Byun) 한국축산학회 1995 한국축산학회지 Vol.37 No.1

        Biochemical polymorphisms of five red cell and semen proteins, Hemoglobin(Hb), Transferrin(Tf), Post-transferrin 2(Ptf2), Post-albumin(Pa) and Albumin(Alb) as genetic markers in Korean cattle were analyzed by Starch and Polyacryamide gel electrophoresis and their phenotypes, genotypes and gene frequencies were estimated in order to analysis the genetic constitution of Korean native cattle population. In the Hemoglobin(Hb) locus four different phenotypes AA, AB, BB and CH were observed and assumed to be controlled by four different alleles designated Hb^A, Hb^B, Hb^C and Hb^H, and the Hb^H type was rare variant of Korean native cattle. The observed distribution of phenotypes were 73.37% for AA type, 23.37% for AB type. 2.72% for BB type and 0.54%r for CH type. Gene frequencies of Hb^A, Hb^B, Hb^C and Hb^H were 0.8505, 0.1440, 0.0027 and 0.0027. Semen Transfetrin(Tf) locus, 11 different phenotypes AA, AD₁, AD₂, AE, AH, D₁D₁, D₁D₂, D₁E, D₂H, D₂D₂, D₂E, EE and EH type were identified, which considered to be controlled by codominant alleles TF,^A Tf^D, Tf^D, Tf^E and Tf^H at a single locus. The frequencies of Tf genotypes AD₁, D₁E, D₁D₂, D₂E, AA, AE, D₁D₂, AD₂, D₁D₁, EE, AH, D₂H and EH were found to be 16.30, 13.33, 11.85, 10.37, 9.69, 8.15, 7.41, 9.63, 5.93, 4.44, 1.48, 0.74 and 0.01%, respectively. Gene frequencies of TF^A, Tf^(D1) Tf^(D2) and Tf^H were 0.2741, 0.2704, 0,2333, 0.2074 and 0.0148, respectively. And TfH gene were newly identified in Korean native cattle. Considering Post-transterrin 2 locus, three different phenotypess FF, FS and SS were identified, which considers to he controlled by two alleles Ptf^F and Ptf^S at a single autosomal locus. The frequencies of Rf genotypes FS, FF and SS were found to be 51.06. 36.88 and 12.06%n, respectively and gene frequencies of Ptf^F and Ptf^S were 0.6241 and 0.3759. In the Postalbumin(Pa) locus, three different phenotypes FF, FS and SS type were observed to be genetically controllled by Pa^F and Pa^S gene. And genotypes frequencies FS. FF amd SS type were 48.65, 36.(H and 1_5.32%, respectively. The gene frequencies of Pa^F and Pa^S were 0.6036 and 0.3964. The Albumin(Alb) locus were observed to lack any individual variation. Therefore, this locus were defined to be monomorphic. In comparison of genetic distance and dendogram calculated from the gene frequencies, close relationship was obtained between the Japanese cattle and the Korean cattle.

      • SCISCIESCOPUS

        Novel substrates of a ribose-5-phosphate isomerase from Clostridium thermocellum

        Yoon, R.Y.,Yeom, S.J.,Kim, H.J.,Oh, D.K. Elsevier Science Publishers 2009 Journal of biotechnology Vol.139 No.1

        A substrate specificity study of the recombinant D-ribose-5-phosphate isomerase (RpiB) from Clostridium thermocellum was performed. Among all aldopentoses and aldohexoses, the RpiB enzyme displayed activity with L-talose, D-ribose, D-allose, L-allose, L-ribose, and D-talose in decreasing order. The products released were L-tagatose, D-ribulose, D-psicose, L-psicose, L-ribulose, and D-tagatose, respectively. The enzyme showed specificity for aldose substrates possessing hydroxyl groups oriented in the same direction at the C2, C3, and C4 positions. Molecular modeling of the enzyme suggests that the novel substrate specificity may be explained by substrate interactions with residues Tyr42, His98, and His9, which interact with the hydroxyl groups of C2, C3, and C4, respectively, oriented in the same direction. L-Talose and D-ribulose exhibited the highest activity among the aldoses and ketoses, respectively. Ribose 5-phosphate isomerase catalyzed the conversion of L-talose to L-tagatose with an 89% conversion yield after approximately 90min, while D-ribulose was converted to D-ribose with a 38% conversion yield.

      • KCI등재

        한국인에서 DXS7132 유전좌와 GATA31D10 유전좌의 다형성에 관한 연구

        안종성,장영길,이숭덕,신창호,이윤성,이정빈 大韓法醫學會 2000 대한법의학회지 Vol.24 No.1

        The validation study for two STR loci on X-chromosome, DXS7132 and GATA31D10, was done including allelic distribution and frequency of each allele to use these results for individual identification and paternity testing. For 496 unrelated Koreans, above two STR loci were amplified simultaneously using duplex PCR amplification method. The amplified products were analyzed by polyarylamide gel electrophoresis followed by silver staining. In male DXS7132 locus revealed 7 different alleles ranging from 276bp to 300bp. The largest allele was consisted of 14 repetition of [TCTA] unit and took 0.3417. The allele 15 followed next as 0.3165 and allele 13 as 0.1726. In female general distribution was same except one allele, allele 18 was found additionally. The heterozygosity was 0.7706 and 23 different genotypes were found. Polymorphism information content(PIC) was 0.727. Two cases of mutation were noted in DXS7132 locus In both male and femal 7 different alleles were noted in GATA31D10 locus and the alleles ranged from 195bp to 231bp. The allele 15(199bp) took the majority of all as 0.825. The other alleles showed rather relatively low frequency. The heterozygosity was 0.2385 and 11 different genotypes were found. PIC was 0.2521, and no mutation was noted in GATA31D10 locus. Considering these two loci together, 22 different halpotype were noted.

      • Exendin-4 induction of cyclin D1 expression in INS-1 beta-cells: involvement of cAMP-responsive element.

        Kim, M-J,Kang, J-H,Park, Y G,Ryu, G R,Ko, S H,Jeong, I-K,Koh, K-H,Rhie, D-J,Yoon, S H,Hahn, S J,Kim, M-S,Jo, Y-H Journal of Endocrinology, Ltd. [etc.] 2006 The Journal of endocrinology Vol.188 No.3

        <P>Glucagon-like peptide-1 (GLP-1) and its analog exendin-4 (EX) have been considered as a growth factor implicated in pancreatic islet mass increase and beta-cell proliferation. This study aimed to investigate the effect of EX on cyclin D1 expression, a key regulator of the cell cycle, in the pancreatic beta-cell line INS-1. We demonstrated that EX significantly increased cyclin D1 mRNA and subsequently its protein levels. Although EX induced phosphorylation of Raf-1 and extracellular-signal-regulated kinase (ERK), both PD98059 and exogenous ERK1 had no effect on the cyclin D1 induction by EX. Instead, the cAMP-elevating agent forskolin induced cyclin D1 expression remarkably and this response was inhibited by pretreatment with H-89, a protein kinase A (PKA) inhibitor. Promoter analyses revealed that the cAMP-responsive element (CRE) site (at position -48; 5'-TAACGTCA-3') of cyclin D1 gene was required for both basal and EX-induced activation of the cyclin D1 promoter, which was confirmed by site-directed mutagenesis study. For EX to activate the cyclin D1 promoter effectively, CRE-binding protein (CREB) should be phosphorylated and bound to the putative CRE site, according to the results of electrophoretic mobility shift and chromatin immunoprecipitation assays. Lastly, a transfection assay employing constitutively active or dominant-negative CREB expression plasmids clearly demonstrated that CREB was largely involved in both basal and EX-induced cyclin D1 promoter activities. Taken together, EX-induced cyclin D1 expression is largely dependent on the cAMP/PKA signaling pathway, and EX increases the level of phosphorylated CREB and more potently trans-activates cyclin D1 gene through binding of the CREB to the putative CRE site, implicating a potential mechanism underlying beta-cell proliferation by EX.</P>

      • Acetylene-bridged D-A-D type small molecule comprising pyrene and diketopyrrolopyrrole for high efficiency organic solar cells

        Mun, J.W.,Cho, I.,Lee, D.,Yoon, W.S.,Kwon, O.K.,Lee, C.,Park, S.Y. Elsevier Science 2013 Organic electronics Vol.14 No.9

        To explore effects of acetylene-incorporation, acetylene-bridged D-A-D type small molecules ((HD/OD)-DPP-A-PY) using pyrene as a donor and diketopyrrolopyrrole as an acceptor were successfully synthesized and characterized. (HD/OD)-DPP-A-PY exhibited planar back-bone, conjugation extension, enhanced light absorption, and low HOMO energy level. Combined with the advanced properties, solution-processed OSCs based on a blend of HD-DPP-A-PY as a donor and [6,6]-phenyl-C<SUB>71</SUB>-butyric-acid-methyl-ester (PC<SUB>70</SUB>BM) as an acceptor exhibited PCEs as high as 3.15%.

      • KCI등재

        한우 경제형질에 미치는 Mitochondrial DNA D-loop 영역의 염기서열 변이효과

        오재돈,윤두학,공홍식,임현진,이학교,조병욱,홍기창,전광주 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

        This study was performed to analyse the sequences of variations of mtDNA D-loop and their effects on carcass traits in Hnawoo(Korean cattle). The resulting sequences were compared with perviously published sequences for other cattle breeds(GenBank J01394). The PCR was used to amplify a total of 964 bp between nucleotide 15758 and 383 within D-loop region of mtDNA using specific primers. Twenty five polymorphic sites by nucleotide substitution were found in mtDNA of Hanwoo. The frequencies of positions at 169, 16042, 16093. 16119, 16255 and 16302 nt with high levels of sequence polymorphism were 0.891, 0.117, 0.109, 0.182, 0.197 and 0.117, respectively. The substitution effect at 169 and 16119 nt was found significant on marbling score. Also substitution effect at 169 and 16042 nt was highly significant(p<0.01) on backfat, thickness. Polymorphisrn of mtDNA sequence in D-loop region could be useful for the analysis of cytoplasmic genetic variation and associations with the other economically important traits and maternal lineage analysis in Hanwoo.

      • SCISCIESCOPUS

        X-ray and electron microscopy studies on the biodistribution and biomodification of iron oxide nanoparticles in Daphnia magna

        Kwon, D.,Nho, H.W.,Yoon, T.H. Elsevier 2014 Colloids and surfaces Biointerfaces Vol.122 No.-

        Biodistribution and biomodification of iron oxide (Fe<SUB>3</SUB>O<SUB>4</SUB> and α-Fe<SUB>2</SUB>O<SUB>3</SUB>) nanoparticles (NPs) in a well-known toxicity test organism, Daphnia magna (D. magna), were investigated using transmission electron microscopy (TEM) and scanning transmission X-ray microscopy (STXM). In addition to the morphological changes in the gut tissues of D. magna, biodistribution and biomodification of iron oxide NPs in the digestive tract of D. magna were also monitored in this study. Upon exposures to both iron oxide NPs, unique morphological changes (e.g., irregular shaped microvilli, epithelial cell protrusion, and dilatation of cytoplasmic inclusion) in the gut tissues of D. magna were observed along with bacterial colonization of the gut lumen. However, despite their heavy accumulations in the digesitive tract, TEM and STXM images confirmed us that both Fe<SUB>3</SUB>O<SUB>4</SUB> and α-Fe<SUB>2</SUB>O<SUB>3</SUB> NPs were not penetrating into the gut tissues of D. magna. Moreover, for the Fe<SUB>3</SUB>O<SUB>4</SUB> NPs in direct contact with the gut microvilli of D. magna, slight but significant spectral changes were observed in their Fe L-edge X-ray absorption near edge structure (XANES) spectra, which indicated that there were biomodifications of Fe<SUB>3</SUB>O<SUB>4</SUB> NPs, probably involving oxidative dissolution of Fe<SUB>3</SUB>O<SUB>4</SUB> NPs followed by rapid precipitation of ferric oxide or hydroxide. However, no significant changes were observed in the Fe L-edge XANES spectra of the α-Fe<SUB>2</SUB>O<SUB>3</SUB> NPs present in the gut lumen of D. magna. These X-ray and electron microscopic observations confirmed us that, despite similarities in core sizes and chemical compositions, NPs with different crystalline phase and dissolution rates can interact quite differently with their local environment, may result in different biodistribution and cause completely dissimilar toxicities.

      • KCI우수등재

        재래돼지 미토콘드리아 게놈내 D-loop 영역의 염기서열 분석

        정일정,김태헌,윤두학,조진기,이효신 한국축산학회 1997 한국축산학회지 Vol.39 No.3

        The objective of this study was to investigate differences in mtDNA D-loop sequence between Korean native pig and other breeds. When digested with BamH I/Cla I, D-loop region was found in 1.03 kb and 1.59 kb fragments. Both fragments containing D-loop region were subcloned into plasmid vector of pBluescript II SK^+ and sequenced. After sequencing 2616 by in this study, D-loop region was 1313 by and the rest of them were genes for cytochrome b, 12S rRNA, tRNA^(Pro) tRNA^(Phe) and tRNA^(Thr). The twenty-eight tandem repeats of 10bp, CGTGCGTACA, were found within D-loop region of mtDNA in Korean native pig. The sequence variation between Korean native pig and Meishan pig was observed for only 3 nucleotides substitution and 2 nucleotides deletion. However, the sequence difference between the Korean native pig and European pig showed 16 nucleotide substitution, 1 nucleotide deletion and 1 nucleotide insertion. The highly variable region among the three breeds was located between tRNA^(Pro) gene and CSB 1.

      • Valeriana officinalis extract and its main component, valerenic acid, ameliorate d-galactose-induced reductions in memory, cell proliferation, and neuroblast differentiation by reducing corticosterone levels and lipid peroxidation

        Nam, S.M.,Choi, J.H.,Yoo, D.Y.,Kim, W.,Jung, H.Y.,Kim, J.W.,Kang, S.Y.,Park, J.,Kim, D.W.,Kim, W.J.,Yoon, Y.S.,Hwang, I.K. Pergamon Press ; Elsevier Science Ltd 2013 Experimental Gerontology Vol.48 No.11

        Valeriana officinalis is used in herbal medicine of many cultures as mild sedatives and tranquilizers. In this study, we investigated the effects of extract from valerian root extracts and its major component, valerenic acid on memory function, cell proliferation, neuroblast differentiation, serum corticosterone, and lipid peroxidation in adult and aged mice. For the aging model, d-galactose (100mg/kg) was administered subcutaneously to 6-week-old male mice for 10weeks. At 13weeks of age, valerian root extracts (100mg/kg) or valerenic acid (340μg/kg) was administered orally to control and d-galactose-treated mice for 3weeks. The dosage of valerenic acid (340μg/kg), which is the active ingredient of valerian root extract, was determined by the content of valerenic acid in valerian root extract (3.401+/-0.066mg/g) measured by HPLC. The administration of valerian root extract and valerenic acid significantly improved the preferential exploration of new objects in novel object recognition test and the escape latency, swimming speeds, platform crossings, and spatial preference for the target quadrant in Morris water maze test compared to the d-galactose-treated mice. Cell proliferation and neuroblast differentiation were significantly decreased, while serum corticosterone level and lipid peroxidation in hippocampus were significantly increased in the d-galactose-treated group compared to that in the control group. The administration of valerian root extract significantly ameliorated these changes in the dentate gyrus of both control and d-galactose-treated groups. In addition, valerenic acid also mitigated the d-galactose-induced reduction of these changes. These results indicate that valerian root extract and valerenic acid enhance cognitive function, promote cell proliferation and neuroblast differentiation, and reduce serum corticosterone and lipid peroxidation in aged mice.

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