Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

고속액체크로마토그래피법에 의한 식품중의 삭카린과 글리시리진의 동시분석

정진일,박유신,박영준,정희선 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

일반 여성과 영양사의 공중화장실 이용 후 손 씻기 실태

문정아,유창희,이경은 서울여자대학교 자연과학연구소 2011 자연과학연구논문집 Vol.23 No.-

The purposes of this study were to examine handwashing practices among dietitians and female adults in public restrooms and to determine environmental factors affecting handwashing. Trained investigators observed 312 women (156 dietitians, 156 female adults) handwashing in 54 public restrooms in Seoul and recorded facilities of the restrooms. The data were collected during May and July, 2009 and analyzed by t-test and χ2-test using SPSS 14.0. More dietitians (91.7%) washed their hands after using public restrooms than the female adults (78.8%) (p<.01). However, more than half of the subjects washed hands without soaps. The dietitians (13.1 seconds) washed hands longer than the female adults (10.1 seconds) (p<.01), but both groups washed hands shorter than 20 seconds, which is recommended for proper handwashing. Both warm and cold water was available at 63% of the observed public restrooms. Significantly more females washed their hands at the restrooms where both warm and cold water was available (88.5%) than where only cold water was available (80%)(p<.05). Literature has revealed that most consumers understand the importance of handwashing. However the finding of the study showed that their handwashing practices in everyday life did not meet their perception. This observation study disclosed the gap which was not reported from the studies conducted based on surveys. From now on, messages for the consumers should focus on when and how their hands should be washed. Finally dietitians should be a role model of proper handwashing in foodservice facilities as well as in everyday life. Key words:handwashing, observation, dietitians, female adults, public restooms.

뮤코펙트 정(염산암브록솔 30mg)에 대한 암브렉트 정의 생물학적 동등성

유정연,정선경,최미희,한상범,이경률,이희주 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

A bioequivalence study of Ambrect^(™) tablets (Dong Wha Pharm. Ind. Co., Ltd.) to Mucopect^(™) tablets(Boe-hringer Ingelheim Korea, Ltd.) was conducted according to the guideline of Korea Food and Drug Administration (KFDA). Twenty four healthy male Korean volunteers received each medicine at the ambroxol hydorchoride dose of 30 ㎎ in a 2×2 crossover study. There was a one-week wash out period between the doses. Plasma concentrations of ambroxol were monitored by a high-performance liquid chromatography for over a period of 24 hours after the administration. Aug_(t)(the area under the plasma concentration-time curve from time zero to 24 hr) was calculated by the linear trapezoidal rule method. C_(max) (maximum plasma drug concentration) and T_(max) (time to reach C_(max)) were compilied from the plasma concentraction-time data. Analysis of variance was carried out using logarithmically transformed AUC_(T) and C_(max). No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the AUC_(t) ratio and the C_(max) ratio for Ambrect^(™)/Mucopect^(™) were 0.89-1.01 and 0.89-1.02, respectively. These values were within the acceptable bioequivalence intervals of 0.80-1.25. Thus, our study demonstarated the bioequivalence of Ambrect^(™) and Mucopect^(™) with respect to the rate and extent of absorption.

여가스포츠 참여와 서비스마케팅 특성과의 관계

유승원,정희석 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

Based on the characteristics of the service marketing and those of the leisure sport population and society, a survey on leisure sport service marketing was carded out regarding the satisfaction of leisure sport participants and workers in the leisure sport industry, The survey aimed to promote the leisure sport industry through understanding the relationship between the leisure sport participation and the characteristics of the service marketing. The subjects of the survey were 574 in total, For the statistical analyses Duncan test and co-variation analysis were done for the verification and to examine the variance differences, The founding of the research was as follows; First, in the relationship of the service marketing characteristics and the kind of leisure sports, there were statistically meaningful differences in basic-service(BS), program-management-service(PMS), effect-service(FS) and general service(GS) regarding sex; in facility-service(FS) and effect-service(FS) regarding the marital status; in basic-service(BS), program-management-service(PMS), facility-service(FS) and general service(GS) regarding the kind of leisure sports, The satisfaction of squash was the highest and that or golf the lowest. Second, in the relationship of the service marketing characteristics and the frequency of leisure sport participation, there were statistically meaningful differences in basic-service(BS) and program-management-service(PMS) regarding sex; in facility-service(FS) and effect-service(FS) arid general service(GS) regarding sex arid the marital status. There were statistically meaningful differences in BS, PMS, FS and GS regarding the independent variable of participation frequencies, The satisfaction of the 'once a month' group was the highest and that of 'almost everyday' group was the lowest.

엘리트 알파인 스키 선수와 대학서클 알파인 선수의 등속성 근력 발현 특성에 대한 비교 연구

유승희,안정현,최현희 경희대학교 체육과학연구소 2001 體育學論文集 Vol.29 No.-

The purpose of this study was to compare isokinetic muscle strength between elite players and college players on alpine skiing and to offer foundamental information on characteristics of thigh muscle strength for elite players and effective training method for college club players The subjects of this study were 10 national ski players and 10 college club players. Also, the experimental instruments used were Martin's anthropometer(PKS-100, Japan), weight meter(TBF-202, Japan), and isokinetic equipment(CYBEX NORM, USA). To analyze the data, the statistical method used in this study were mean, standard deviation, and t-test using SPSS for Windows(Version 10.0.7). Based on aforementioned research method, the results of this study are as follows. 1. In peak torque, elite group was higher than college club group. Especially, right extension(p〈.05) and right flexion(p〈.01) were significant high in 240˚/sec. 2. In peak torque % of body weight, elite group was significant high on riggtㆍleft extension and flexion in 60˚/sec & 240˚/sec. 3. In angle of peak torque, elite group was higher than college club group. Especially, left extension(p〈.01) was significant high in 60˚/sec. 4. In average power, elite group was higher than college club group. Especially, right extension(p〈.05) and right flexion(p〈.01) were significant high in 240˚/sec. 5. In total work, elite group was significant high on rightㆍleft extension and flexion. However, on the muscle endurance ratio, there was no significant difference. 6. In peak torque for flexion/extension, there was no significant difference between elite group and college club group.

민들레 잎과 뿌리 분말을 첨가한 설기떡의 품질 특성

유경미,김세희,장정화,황인경,김경임,김성수,김영찬 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1

In order to develop a dandelion (Taraxacum officinale) with natural food applications, the effects of dandelion leaves and roots powder content on the physical, textural and sensory properties of sulggidduk were examined. Dandelion powders of leaves and roots at 0, 1, 2 and 3% were added to the dandelion sulgidduk. In the dandelion roots powder in sulgidduk, the color intensity of L values and the values and moisture content decreased however, the sensory color intensity, dandelion flavor and overall acceptability increased with increasing the dandelion powder content. The sensory overall acceptability indicated the dandelion roots powder content at 3% had the highest overall and flavor scores. As the dandelion leaves content was increased, the moisture contents, lightness, adhesiveness and cohesiveness of sulggidduk decreased, while the sensory color intensity, sensory hardness, hardness of texture properties and dandelion flavor increased. The addition at 1% of the dandelion leaves powder content with the dandelion sulggidduk and the addition at 3% of the dandelion roots powder content with the dandelion sulggidduk showed the highest overall acceptability.

진행성 위암환자에서 Capecitabine/Cisplatin 치료군과 Fluorouracil/Cisplatin 치료군 간의 삶의 질 비교

유희원,신은정,이광자,한현주,손인자 韓國病院藥師會 2004 병원약사회지 Vol.21 No.1

The improvement of medical treatment and anticancer drug has led a complete recovery and prolonged survival time of cancer patients. And recent study of cancer has included evaluation of quality of life. This study was performed to evaluate quality of life between Capecitabine/Cisplatin arm and Fluorouracil/Cisplatin arm. This study was performed in the Seoul National University Hospital from August to October 2003. A total of 8 AGC patients on adjuvant chemotherapy successfully completed this study. Method of study is questionnairing by EORTC QLQ-C30, cancer specific questionnaire. The questionnaire consists of Functional, Symptom, Global health quality of life scale. Patients eligible for this study had histologically confirmed stage III or IV AGC. Patients were to be between 18 and 60 years old, and have performance status of 1. Patients were ineligible if they had a history of 6 months prior or concomitant chemotherapy, radiotherapy. Statistically significant differences favoring the Fluorouracil/Cisplatin arm was noted in the physical scale. In addition, patients in the Fluorouracil/Cisplatin arm reported an improvement in physical functioning after one cycle chemotherapy.

두개골의 3차원 영상 분석을 위한 전산화단층촬영 방법의 비교 : 상층 두께가 3차원 영상의 계측에 미치는 영향

정호걸,김기덕,박혁,김동욱,정해조,김희중,유선국,김용욱,박창서 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.3

Purpose : To evaluate the quantitative accuracy of three-dimensional (3D) images by means of comparing distance measurements on the 3D images with direct measurements of dry human skull according to slice thickness and scanning modes. Materials and Mathods : An observer directly measured the distance of 21 line items between 12 orthodontic landmarks on the skull surface using a digital vernier caliper and each was repeated five times. The dry human skull was scanned with a Helical CT with various slice thickness (3, 5, 7 mm) and acquisition modes (Conventional and Helical). The same observer measured corresponding distance of the same items on reconstructed 3D images with the internal program of V-works 4.0 (Cybermed Inc., Seoul, Korea). The quantitative accuracy of distance measurements were statistically evaluated with Wilcoxons’ two-sample test. Results : 11 line items in Conventional 3 mm, 8 in Helical 3mm, 11 in Conventional 5 mm, 10 in Helical 5 mm, 5 in Conventional 7 mm and 9 in Helical 7 mm showed no statistically significant difference. Average difference between direct measurements and measurements on 3D CT images was within 2 mm in 19 line items of Conventional 3 mm, 20 of Helical 3 mm, 15 of Conventional 5 mm, 18 of Helical 5 mm, 11 of Conventional 7mm and 16 of Helical 7 mm. Conclusion : Considering image quality and patient’s exposure time, scanning protocol of Helical 5 mm is recommended for 3D image analysis of the skull in CT.

재발성 류마티즘 환자에서 발작의 유발인자

주유철,이동민,양종태,신병철,임태영,서정균,정기영,최동헌,김원,조영신,고희관 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : Palindromic rheumatism (PR) is known to be triggered without any obviously inciting events in most patients, although, in a few cases, attacks have been noted to relate to another events such as weather change, childbirth, ingestion of certain foods, or over-exercise. The aim of this study was to characterize the triggering factors on palindromic attacks. Materials and Methods : We evaluated sixty-four patients with PR for the presence of triggering factors and the relationship between attacks and triggering factors by interview of patients. We considered that there was an probable association between episodes and PR, if episodes within 24 hours prior to PR had triggered at least 50% of all attacks and a definite association between episodes and PR, if episodes within 24 hours prior to PR had triggered 100% of all attacks. Results : In 15 patients (35.9%), there was a possible association between episodes of PR and initiating events. In 8 patients (12.5%), there was a definite association between episodes of PR and initiating events. The triggering factors were physical over-activity including exercise (19/64: 29.7%), foods, such as rawfish, crab, and chicken, including alcohol (19/96: 19.8%). However, there were no difference between the groups with and without the triggering factor in sex ratio, duration and onset of PR, the positive rate of rheumatoid factor and involved sites. Conclusion : Our observations showed that PR was triggered by physical over-activity more than in previous reports and hypersensitivity to foods was a causative factor in some patients with PR. However, patients with triggering factors had a similiar clinical profile to patients without triggering factors.