내산성 및 내담즙성이 우수한 양돈용 유산균의 선발

유자영,양시용,송민동 建國大學校 自然科學硏究所 2002 建國自然科學硏究誌 Vol.13 No.1

본 연구는 가축의 장내에서 유익한 영향을 주는 유산균을 돼지의 분변으로부터 분리하고 내산성과 내담즙성이 뛰어난 균을 선별하여 유산균 생균체를 준비하고자 실시하였다. 분변에 섞여 있는 다른 균들과 산이나 담즙액에 약한 균들은 처음부터 분리하기 위하여 pH를 2.0으로 맞춘 MRS broth에 배양시키고 oxgall이 첨가된 배지에 도말하여 40개의 균주를 분리하였다. BCP배지를 이용해서 유산의 생성여부를 확인하고, O.D값과 pH를 측정하였으며, 그람염색과 형태관찰을 통하여 33개의 균주를 분리하였다. 분리된 균주중에서 산에 대한 내성이 있다고 판단되는 12개의 균주를 분리해냈고, 12개의 균주의 내산성 및 내담즙성을 3회에 걸쳐 시험하고 내산성과 내담즙성이 우수한 3개의 균주를 선별하였다. 마지막으로 담즙산 농도별 시험을 행함으로써 가축의 장까지 살아서 도달하기 위해 꼭 갖추어야 할 조건인 내산성과 내담즙성이 가장 좋은 #32-1 균주를 최종 선발하였다. 유산균이 생균제로서의 기능을 수해하려면 oxgall이 0.3% 함유된 배지에서 성장할 수 있을 정도의 내성을 갖고 있어야 한다는 보고가 있는데 #32-1 균주의 경우 담즙액의 농도가 3%인 배양액에서도 높은 생존률을 보임으로써 생균제로서 사용하기위해 갖고 있어야 할 담즙에 대한 내성이 우수하다고 판단하였다. This study was performed to screen lactic acid bacteria for the direct-fed-microbials. A lactic acid bacteria which has resistant to acid and bile salts were isolated from swine feces. Among the previously obtained acid tolerant strains, three of them show resistant to artifical gastric juice and bile. Finally, 321- stain was selected for good growth an high survival rate. The finally selected 32-1 stain was identified as Lactobacillus salivarius. The strain, which named L. salivarius JY 32-1, was able to tolerate acid condition down to pH 2.5 and could also tolerate bile at 3%.

건강한 한국인에서의 배변 조영술 소견

나용호,장명규,김태현,김형언,유광현,진경민 大韓消化器病學會 1995 大韓消化器病學會誌 Vol.27 No.3

국내 주요 상수원수와 처리수에서 HAAs 생성 특성

염철민,최유식,변석종,조순행,윤제용 대한상하수도학회 2002 상하수도학회지 Vol.16 No.2

This study was conducted to investigate the haloacetic acid (HAAs) formation characteristics in raw and treated water in Korea. The raw and treated water from the five major rivers were examined to measure the HAAs concentrations and compared those with literature values. Major findings are as follows. First, average HAAs concentration of treated water (collected from three water treatment plants) in Korea appeared to be 62.5±66.7㎍/L (n=7) and this level is higher than the average trihalomethanes (THMs) concentration (13.2±12.9㎍/L, n=6933) reported recently. Second, the average formation potential of TCAA and DCAA after three days was found as 103±74㎍/L (n=14) and 76±36㎍/L (n=14), respectively. As this result was applied to US-EPA developed model equation for TCAA and DCAA formation, dissolved organic matter (DOM) in Korea has a tendency to produce more TCAA and DCAA as compared to US. This phenomenon was especially notable in DCAA. The sum of weight percentage of TCAA and DCAA which are individual component of HAAS was composed of 80% of HAAs.

냉각탑용 수분배기의 특성에 관한 연구

전유신,최석천,이용훈,김세현,정효민,정한식 경상대학교 해양산업연구소 2002 해양산업연구소보 Vol.15 No.-

The main purpose of this research is the investigation of water distributer. The water distributer of this research is installed in evaporative cooling tower system. The performance of this cooling tower system depend on the evaporative surface, and there are many kinds of methods for increasing the evaporative surface. In this research, we introduced the two kinds of spraying system, one is the hole type with hole diameter 1, 2 and 3mm, and the other is the spray nozzle types with nozzle tip diameter of 1 and 2mm. As the experimental results, the case of diameter 2mm showed the best performance.

호스피스 병동과 일반병동의 말기암환자의 간호중재 비교

노유자,한성숙,용진선,송민선,홍진의 성인간호학회 2002 성인간호학회지 Vol.14 No.4

Purpose: The purpose of the study was to compare symptoms, medical therapies, and nursing interventions with terminal cancer patients during the last four weeks of their lives in a hospice unit and general units. Method: For the descriptive survey study, data were collected by reviewing the medical records of 243 patients who died of terminal cancer at K hospital in Seoul. The data was analyzed by using Chi-square test and t-test. Result: The study findings are summarized as follows. There were higher frequencies in physical symptoms of constipation, itching sensation, pain, sleeping disturbance, soreness and dysuria for those patients in the hospice unit than those patient in general units. All emotional symptoms were recorded significantly higher for those patients in the hospice unit than those in general units. Regarding the major medical interventions, pain management was used more significantly for those patients in the hospice unit, but antibiotic therapy and resuscitation were used more significantly for those patients in general units. Conclusion: The hospice unit provided more comprehensive nursing interventions including psychological, spiritual, and family cares as well as physiological care for terminal cancer patients. The facts showed that those patients who would need hospice care in general units should be referred to the hospice unit at an appropriate time.

호스피스 병동과 일반병동의 말기암환자의 간호중재 비교

노유자,한성숙,용진선,송민선,홍진의 가톨릭대학교 간호대학 호스피스 교육연구소 2002 호스피스논집 Vol.6 No.-

The purpose of the study was to compare symptoms, medical therapies, and nursing interventions with terminal cancer patients during the last four weeks of their lives in a hospice unit and general units. For the descriptive survey study, data were collected by reviewing the charts of 243 patients who died of terminal cancer at K hospital in Seoul. The data was analyzed using Chi-square test and t-test. The study findings are summarized as follows: There were higher frequencies in physical symptoms of constipation, itching sensation, pain, sleeping disturbance, sore, and urinary difficulty for those patients in the hospice unit than those patient in general units. All emotional symptoms were recorded significantly higher for those patients in the hospice unit than those in general units. Regarding the major medical treatments, pain control was used significantly more for those patients in the hospice unit but antibiotic therapy and resuscitation were used significantly more for those patients in general units. The hospice unit provided more comprehensive nursing interventions including psychological, spiritual, and family cares as well as physiological care for terminal cancer patients. The facts showed that those patients who need hospice care in general units should be referred to the hospice unite at an appropriate time.

N-Arylnortropinone 유도체의 합성연구

李龍均,朴유미,鄭大一,金寅植,朴鍾勳,盧信愛,鄭逸守,朴敏洙 東亞大學校附設基礎科學硏究所 1998 基礎科學硏究論文集 Vol.15 No.1

Tropinone 화합물들은 색다른 고리계의 존재로 아주 오래전부터 약리학적으로 매우 흥미로운 연구과제로 주목되어 최근에 그들의 합성법,?? 입체적 구조분석?? 그리고 약리학적인 연구??가 활발히 진행되고 있다. Tropinone 1은 흔히 마약으로 알려져 있는 cocaine 2, 소염작용과 항경련성 작용 및 심장박동수를 조절 등 다양한 약리작용을 하는 tropane spirohy-dantoin ?? 그리고 안과질환을 검사 할 때 눈의 동공을 확장시키는 데 사용하는 atropine ??등을 합성하는 데 중요한 중간체이다. ??????????? Trigo, G, G등 ??은 aliphatic amine과 succinic aldehyde를 acetonedicarboxylic acid와 반응하여 nortropane 유도체를 합성하였으며, 그 후 [3+2] nitrone cycloaddition? 과 [3+4] cyclocoupling? 을 거치는 tropane alkaloid의 새로운 합성법이 개발되었다. 최근 lzquier M. L. 등¹은 acetonedicarboxylic acid를 사용하여 1차 지방족 amine과 succinic aldehyde대신 2,5-dimethoxytetrahydrofuran을 반응시켜 N-alkyl nortropinone 유도체를 합성하였다. 그러나 일반적으로 빙초산 속에서 amine과 2,5-dimethoxytetrahydrofuran을 반응시키면 pyrrole 유도체가 합성되어진다.?? 이러한 사실은 분명히 반응조건(예, 산, 온도)에 따른 다양한 화학이 존재한다고 볼 수 있다. 따라서 우리는 반응조건, 즉 산의 변화 및 온도 변화에 의한 amine과 2,5-dimethoxytetrahydrofuran의 반응을 자세하게 검토하면서 새로운 N-arylnortropinone 유도체를 합성하고자 한다.

항문 연축증(Anismus) 환자에서 Biofeedback 훈련의 치료효과

유광현,나용호,진경민 대한대장항문학회 1996 Annals of Coloproctolgy Vol.12 No.2

저압 증발형 압력 용기의 응력 변화 특성에 관한 연구

최석천,이용훈,전유신,허선철,정효민,정한식 경상대학교 해양산업연구소 2002 해양산업연구소보 Vol.15 No.-

The purpose of this research is to obtain the characteristics of strain and stress in the vacuum pressure vessel. The vacuum pressure vessel is used to many of industrial devices and cooling tower system. The vessel is under the vacuum pressure in this research, and we analyzed the stress on the vessel surface. As the experimental set up, we introduced the Static Strain Measurement(UCAM 70A, Kyowa) system. The environmental condition of vessel is reserved under vacuum pressure 50mmHg, and the vacuum pressure was made by a ejector pump system.

HPLC를 이용한 백삼,홍삼 및 홍삼 농죽액의 Ginsenosides 함량 분석

맹주양;이용구;이숙재;정진아;김상민;김유정;육영난 순천향대학교 기초과학연구소 2010 순천향자연과학연구 논문집 Vol.16 No.2

Commercial White ginseng(WG), Red ginseng (RG), and Red ginseng concentrate(RGC) were analyzed for ginsenosides Rg1, Rb1, and Re by HPLC. The contents of Rg1, Rb and Re were 0.25%, 0.17%, 0.13% in WG, the contents of Rg1, Rb1, and Re were 0.11%, 0.23%, 0.12% in RG, and the contents of Rg1, Rb1, and Re were 0.7%, 0.04%, 0.42% in RGC. In Rg1, the contents were decreased in the order of RGC(O.70%)>WG (0.25%)>RG(O.11%), in Rb1, RG(O.23%)>WG(O.17%)> RGC(O.04%), in Re, RGC(0.42%)>WG(0.13%)>RG(O.12%). The total content s of Rg1, Rb1, and Re were decreased in the order of RGC(1.16%)>WG(0.55%)>RG(O.46%). Overall results demonstrated that The content of Rg1 was higher than those of Rb1 and Re in WG and RGC, while the content of Rb1 was higher than those of Rg1 and Re in RG.