자주색 광합성 세균의 수소 생성

趙鏞雲 진주산업대학교 농업기술연구소 1992 農業技術硏究所報 Vol.5 No.-

1989년도 미국 중북부의 Wisconsin주 Millwaukee주변 호수에서 채취분리한 혐기성 자주색광합성 세균중에서 수소 개스를 생성하고 whey폐수중에서 생육하여 정화에 이용할 수 있는 균주를 얻을 목적으로 실험을 한 결과는 다음과 같다. 1) 분리균주인 AT, A10, D22, 및 F12가 maleic acid, glucose를 함유한 배지에서는 생육하였고 lactose에서는 생육하지 않았다. 2) 생육조건으로 무기염을 필요로 하였으며, pH 6.8, 30℃에서 생육 상태가 양호하였다. 3) Glutamic acid를 함유한 경우가 개스를 많이 생성하였다. 4) AT균주가 생성한 개스 성분에서 수소가 47%이었다. In order to obtain the strains that produces hydrogen and can be used to clarify the whey-waste water of anaerobic purple photosynthetic bacteria, we picked and selected in lake of Milwaukee in Wisconsin state of U.S.A. in 1989. The results were as followed. 1. Selected strains, such as AT, A10, D22 and F12, grew well in maleic acid and glucose contained OMGEL medium but did not grow in lactose contained the medium. 2. Selected strains needed inorganic salts, and their growth conditions were good in pH 6.8 and 30℃. 3. Production of gas was much in addition of glutamic acid than controlled. 4. The hydrogen was 47% of produced gases in AT strain.

好熱性 細菌內의 Lactate Dehydrogenase에 關한 性質

趙鏞雲 진주산업대학교 1984 論文集 Vol.22 No.-

LDH효소의 安定性이 보다 높은 온도의 것을 얻기 위하여 好熱性細菌中의 活性이 높은 菌株를 自然에서 채취하여 기본성질을 조사하였다. 1. 온도에 의한 효소활성은 60℃에서 가장 높았고 30℃,80℃에서는 현저한 활성의 감소가 있었다. 2. 열안정성 : 60℃에서는 120분간 처리시까지 변화가 적었으나 70℃,80℃에서는 20분 경과시 50%이상의 효소가 실활되었다. 3. pH에 의한 영향 : pH7.6의 tris­HClbuffer solution일때가 가장 높았다. 4. pH안정성: 중성보다 ph5.8에서 가장 안정하였고 pH 5.8∼8.0사이에서는 비교적 안정하였다. 5. 균체량 : 0.5%의 glucose를 넣은 배지가 가장 량이 많았고 tryptone을 함유한 배지에서 균체량이 높게 나타났다. Heat­stable lactate dehydrogenase (LDH) was obtained from an thermophilic bacterium which selected by hot waste water or compost in Chinju area. 1. In case of LDH Crude enzyme from thermophilic bacterium. 1) Optimum temperature was at 60℃ but 30℃ and 80℃ were very decreased on enzyme activity. 2) Heat­stability: The treatment at 60℃ was non­sensitive for 120min, heat stability of crude enzyme at 70℃ and 80℃ was 50% relative activity for 20minutes. 3) Optimum pH: Enzyme activity was maximum tris­HC1 buffer in pH 7.6. 4) pH stability: pH was relatively stability at pH 5.8∼8.0 and maximum pH 5.8 at 60℃ for 3.5hours. 2. The effect of cell growth when it incubate with variety medium. Much cell was cultured media with 0.5% glucose or tryptone than That of peptone source.

유소아 응급처치시 Ketamine hydrochloride의 효과

조남수,이경운,김용배 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Our study was made on 96 cases of ketamine anesthesia, who visited to emergency department of Chosun university hospital. This patient was not controlled by verbal order and lidocaine anesthesia, and indication for the use of ketamine(from 1 to 5 years old). Dosage of ketamine in the intramuscular injection was 5mg/kg, in the intravenous injection was 2mg/kg. The results were as follows; 1. After the ketamine injected, expending time doing induction was for 2∼4 minutes in cases of intramuscular injection, and expending time doing induction was for 20∼60 seconds in cases of intravenous injection 2. After the ketamine injected, patient`s awake time was 50±5 minutes in case of intramuscular injection, and that is 20±5 minutes in cases of intravenous injection. 3. Injected ketamine caused increased blood pressure, pulse rate, and respiration rate, and saturation of oxygen was maintained 92-98% level. 4. In adverse effect, the presence of spontaneous eye opening, mild a groan, purposeless involuntary movement and increased excretion of saliva was appeared. 5. Advantage of ketamine injection was that one attendant was enough for restraining the patient, and reduced pain sense, and operator performed various procedures easily on stable condition. From the above results, it was considered in emergency department that indications for ketamine anesthesia are primary closure of simple facial laceration, burn dressing, and some diagnostic procedures( cardiac & ureteric catheterization, cystoscopy) in pediatric patients. It was considered, that all procedures should be performed under fullfill all the condition such as professional control devices(esp. central monitoring system) and basic CPR instruments and ketamine anesthesia-skilled doctor.

西部慶南地域의 느타리버섯 裁培 및 疾病 發生 現況

김철호,조용운,김홍출,이상원 진주산업대학교 1999 論文集 Vol.38 No.-

The authors surveyed the present situation of Agaricus sp cultivation and its disease occurence in Western Kyong-Nam area. 192 farmers, which is 66% of the mushroom-cultivating farmers, were growing Agaricus sp. Especially there were 124 farms in Western Kyong-Nam around Chinju-City. The greater part of mushroom-farmers cultivated Agaricus sp. on tables using cotton as major medium. And they purchased the eldest mycelium from other districts. About 60% of the farmers were suffering some losses due to the development of disease. The major disease seemed to be brown blotch. When a mushroom-disease has developed, most farmer coped with it by removing the cotton media, and sometimes by treating with chemicals. Mushrooms seemed to be a major source of farmers' income, and some govenmental and scientific supports are needed for the development of mushroom cultivation.

공역 리놀레산 유도체의 세균생장 억제효과

金紅出,趙鏞雲,李相元,金哲鎬 진주산업대학교 2000 산업과학기술연구소보 Vol.- No.7

Antibacterial activity of CLA-K salt was surveyed in this paper. CLA-K(10%) exhibited antibacerial activity at the concentration more than 60 ㎕. The antibacterial activity of CLA-K in liquid culture was relatively proportional to its concentration. The resistance of bacteria against CLA-K increased as time goes by. Streptococcus mutans, Bacillus cereus KCCM-l1204, Lactobacillus plantalium and Escherichia coli ATCC-11835 were relatively sensitive to CLA-K. Staphylococcus aureus KCTC-l927 and Klebsiella pneumoniae KCTC-2208 sensitively reacted to the concentration of CLA-K.

참깨의 脂質變化에 關한 硏究 : 第4報. 貯藏中 sterylglycoside의 sterol 組成變化 4. Changes in Sterol Compositions of Sterylglycoside during Storage

崔相道,趙鏞雲 진주산업대학교 1985 論文集 Vol.23 No.-

참깨 種子油中 sterylglycoside의 含量은 總 sterol의 33.3%로서 2.46㎎/g이였고 sterylglycoside의 分劃別 sterol收得率은 4-desmethylsterol이 32.8%, 4-monomethylsterol이 30.2%, 4,4-dimethylsterol은 16.4%이였다. sterylglycoside의 構成 sterol은 46.8%의 sitosterol, 9.6%의 Δ^5-avenasterol, 9.0%의 campesterol 및 8.0%의 stigmasterol이였고 RRT가 1.36인 未知 sterol이 24.6%이였다. 貯藏中 campesterol의 變化는 LA, LS 및 DA에서는 4個月까지는 약간 增加하였고 그 以後 LS에서는 급격히 감소하였으나 LA 및 DA에서는 變化가 없었고 DS에서는 4個月 以後부터 약간 增加하였으며 stigmasterol은 LA, LS 및 DA에서 4個月까지는 增加하였으나 그 以後 9個月까지 감소하였다. 또한 sitosterol은 LA, LS 및 DA에서는 4個月까지는 급격히 감소하고 그 以後는 變化가 거의 없었고 DS에서는 그 反對現象이였으며 Δ^5-avenasterol은 LS에서는 4個月까지는 거의 變化가 없고 그 以後는 감소하였으며 LA 및 DS에서는 4個月까지는 조금 감소하였다. 그 이후는 變化가 거의 없었다. 그리고 未知 sterol은 모든 貯藏條件에서 增加하였으며 LA 및 DA에서는 他貯藏區에 比해 4個月 以後에는 증가폭이 낮았다. The content of sterylglycoside in sesame oil was found to be 2.46㎎ per gram on basis which in 33.3% of total sterol(7.38㎎), and the yield of four fraction from unsaponifiables of sterylglycoside by thin layer chromatography on silica gel G-60 was as follows: 4-desmethylsterol; 32.8, 4-monomethylsterol; 30.2, 4,4-dimethylsterol; 16.4 and less polar compounds; 20.6%. The composition of 4-desmethylsterol in sterylglycoside by gas liquid chromatography was as follows: sitosterol; 46.8, 5-avenasterol; 9.6, campesterol; 9.0 and stigmasterol; 8.0%, and the unknown sterol(RRT: 1.36) consist 24.6%. On the changes of sterol composition in 4-desmethylsterol fraction of sterylglycoside during the storage of sesame seed, the campesterol content in stored LA(air under light), LS(sealing under light) and DA(air in dark) was slightly increased upto 4 months storage period and that in LS was then sharply decreased until 9months storage, but that in DS(sealing in dark) was slightly increased from 4 to 9 months storage period. The stigmasterol in LA, LS and DA was increased upto 4 months storage and then decreased until 9 months storage period. The sitosterol in LA, LS and DA was sharply decreased upto 4 months storage and then not nearly chaged until 9 months storage, but reverse tendency was observed in DS. The 5-avenaterol in LS was hardly changed upto 4 months storage and then decreased until 9 months storage period, and that in LA and DS was a little decreased upto 4 months storage and not much changed until 9 months storage after that. The unknown sterol was sharply increased in all the storage conditions, but the range increased in LA and DA was observed to be lower than that of other storage conditions during the storage period; from 4 to 9 month.

晋州市 南江水質의 變化와 그 影響

金炯甲,趙鏞雲 진주산업대학교 농업기술연구소 1989 農業技術硏究所報 Vol.2 No.-

1988年 6月부터 12月까지 7個月 동안에 걸쳐 晋州市內의 南江 5개 지점을 매월 4∼5 일씩 採水하여 水質을 分析한 結果는 다음과 같다. 調査期間中 南江의 位置別 水質平均値는 1. 下水가 流入되지 않는 上位쪽은 各各 DO 8.8ppm, BOD 2.6ppm, SS 22.1ppm, 蒸發殘溜物 57.4ppm, KM_nO_4 消費量 8.6ppm, 硬度 67.1ppm, Cl^- 10.8ppm이었다. 2. 下水가 流入되어 흐르는 下位쪽은 各各 DO 6.3ppm, BOD 3.8ppm, SS 23.6ppm, 蒸發殘溜物 102ppm, KM_nO_4消費量 12.1ppm, 硬度 73.0ppm, Cl^- 14.2ppm으로서 比較的 汚染이 적었다. 3. 강의 中間에 位置하며 下水가 放流되는 지점은 一部地域이지만 심한 汚染度를 나타냈다. During the seven months from June to December in 1988, Water quality analyzed at five site of the Nam river in Chinju city. Analytical water sampled for four or five days of every month. For investigated preiods, the averages of water quality of the Nam river were as followed. 1. The upstream in which sewage didn't flow into were DO 8.8ppm, BOD 2.6ppm, SS 22.1ppm, total residue 57.4ppm, total hardness 67.1ppm, consumption of KMnO_4 8.6ppm and Cl^- 10.8ppm. 2. The downstream were DO 6.3ppm, BOD 3.8ppm, SS 23.6ppm, total residue 102ppm, total hardness 73.0ppm, consumption of KMnO_4 12.1ppm and Cl 14.2ppm. These showed the low pollution relatively. 3. Though the middle sewage inflow site was a small part, it seemed to be very polluted.

Rhizopus japonicus와 Zymomonas mobilis의 혼합 고정화 배양계를 이용한 생전분으로부터 비살균처리에 의한 에탄올 발효

이상원,조용운 진주산업대학교 1998 論文集 Vol.37 No.-

Rbizopus japonicus와 Zymomonas mobilis로 구성된 혼합고정화 배양계(R-Z 24계)의 특성을 이용하여 biomass로부터 유용물질 생산을 행할 때 투입되는 에너지를 절약할 목적으로 비가열살균 배양을 검토하였다. R-Z 24계 만으로의 비가열살균 배양은 배지에 함유된 세균 때문에 어려웠다. Sorbic acid, benzoic acid, dehydroacetic acid, P-hydroxybenzoic acid와 Vantocil IB등의 여러 가지 살균제 중에서 0.1% Vantocil IB가 잡균의 생육을 억제하는데 가장 적합하였다. Vantocil IB는 곰팡이보다 세균에 대하여, 또 고정화 배양보다 액체배양에서 더 강한 효과를 나타내었다. 0.1% Vantocil IB를 첨가한 비가열살균 배양에서 2, 5 및 10%의 생전분 기질로부터 각각 6.5g/ℓ(Yp/s=0.38), 14.5g/ℓ(Yp/s=0.36) 및 28g/ℓ(Yp/s=0.33)의 에탄올이 생산되어 살균제를 첨가하지 않은 대조구와 동등한 수율을 얻었다. In order to reduce energy input in direct ethanol production from raw starch without sterilization, (co-immobilized mixed Rbizopus japonicus and Zymomonas mobilis culture system(R-Z 24) which changed to anaeoric from aerobic condition after 24hrs of fermentation was investigated.) R-Z 24 system can not be used for ethanol production because of growth of microbial contaminants from medium under nonstrile condition. 0.1% Vantocil IB was the most effective food additive to suppress the growth of contaminants among sorbic acid, benzoic acid, dehydroacetic acid and p-hydroxybenzoic acid. Microbial growth inhibition of vantocil IB was more effective in a bacteria than in a mold, and also in free culture system than immobilized culture system. By adding 0.1% Vantocil IB to the fermentation medium without sterilization, ethanol production was 6.2g/ℓ (Yp/s=0.35), 14.5g/ℓ (Yp/s=0.36) and 28g/ℓ(Yp/s=0.31) from 2, 5, 10% untreated raw starch respectively. These productivities were similar to those of control culture system without Vantocil IB.

수돗물과 정수기물의 미생물학적 수질

김철호,조용운,김홍출,이상원 진주산업대학교 1999 論文集 Vol.38 No.-

The author examined microbiological water quality in Chinju National University. Water temperature was lowest at site 1 as 9.45℃, and highest at site 7 as 26.11℃ on average. pH was neutral being in the range of pH 6.28∼7.75. There was a little relationship between sampling sites and sampling time. General bacterial number exceeded the standard of water quality in every sites. And not a few bacteria were detected in the purified water. Coliform bacteria were detected at every sites and the number increased after rainfall. These results suggest that the water quality should be appropriately managed, although the present microbiological water qualigy is not so serious a problem.

대청호에서 수화(water-bloom)와 대장균군 분포도의 상관관계

김철호,조용운 진주산업대학교 환경문제연구소 1998 環境硏究論文集 Vol.2 No.-

중부지방의 중요한 수자원인 대청호의 회남교 부근에서 수화가 형성되는 시기를 전후하여 대장균군의 분포도를 조사하고, 다른 환경요인과의 상관관계를 확인하였다. 이 수역에서는 조사기간 동안 표층보다는 중층과 하층에 더 많은 대장균군이 분포하였다. 특히 수온약층이 형성되는 시기에는 중층에서 더 높은 분포도를 나타내다가 수온약층이 파괴되면서 수층간에 큰 차이 없이 거의 일정한 분포도를 보였다. 또한 강우량이 많은 시기인 6월 19일과 8월 5일에는 중층과 하층에서 대장균군의 분포도가 높게 나타났는데, 이는 유역의 오염원을 함유하고 있는 상류의 유입수가 수온약층의 하부로 흐름으로써 나타난 결과로 볼 수 있다. 또한 표층에서의 대장균군 분포도는 시안세균에 의한 수화가 형성되는 시기에 비하여 다른 시기에 비교적 높게 나타났는데, 이는 시안세균의 높은 광합성 결과로 표층의 pH가 높아짐으로써 대장균군의 생장이 억제된 결과로 이해할 수 있다. 이상의 결과로부터 자연수계의 대장균군은 외부에서 유입되는 것이 대부분이며, 시안세균의 활성이 높은 시기에는 그 분포도가 낮아진다는 것을 알 수 있다. 이러한 점에서 수화와 같이 환경의 변화가 심한 시기에 자연수계의 분변오염 여부를 대장균군의 분포도만으로 예측하는 것은 한계가 있다고 하겠다. The authors surveyed the monthly variations of distribution of coliform bacteria in Daechung Dam reservoir during cyanobacterial water bloom, and examined its relationship with environmental factors. The distribution of coliform bacteria was higher at middle and bottom layer than at surface layer. Especially its distribution at middle layer was high during the period of thermal decline, and showed little difference in population size of coliform bacteria when thermal decline destroyed. At June 18 and August 5, the period of higher rainfall, the coliform bacteria distributed highly at middle and bottom layers, which seemed to be the result of inflow of low-temperature water. At surface layer, coliform bacterial distribution was higher during water-bloom period, which can be explained by the result of higher pH of surface water resulted from strong photosynthetic activity. These results show that the coliform bacteria in natural water ecosystem originate from the inflow of water and its distribution is low during the period when cyanobacteria grow actively. This suggests that it have a problem to estimate whether the natural water is contaminated with feces.