제1형 당뇨병 환자에서 췌도세포 동종이식의 반복시행

양태영,정인경,서인아,오은영,조건영,오승훈,김성주,정재훈,민용기,이명식,이문규,김광원,도영수,주성욱 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.4

연구배경:췌도세포 이식은 시술이 간편하고 안전하기 때문에 반복이식이 가능하여 그 동안 여러 센터에서 반복이식의 성공을 보고한 바 있다. 국내에서도 본 병원을 비롯하여 몇몇 센터에서 췌도세포 이식이 활발히 시도되고 있으나 사람에서의 동종이식 및 동종 이식의 반복시행은 보고된 바 없는 실정이다. 저자 등은 국내 최초로 췌도세포 동종이식을 시행하여, 혈청 C­펩타이드가 증가하고 인슐린 요구량이 감소하였으며, 혈당농도와 당화혈색소의 안정을 보였다. 그러나 이식 70일 후 다시 인슐린 요구량이 증가하고 C­펩타이드가 감소하여 췌도세포 이식을 다시 시행한 바 있다. 2차 이식 후 70여일이 지난 현재 다시 혈청 C­펩타이드가 증가하고, 인슐린 요구량이 더 감소되어 췌도세포 이식에서 반복 시행의 유용성을 보고하는 바이다. 방법:환자는 32세 남자로 17년전 당뇨병 진단 후 인슐린 치료 중이었으며, 3년전 부터는 만성신부전증으로 혈액툭석을 하고있었다. 1999년 12월 25일 신장이식을 하였고 3일 후 췌도이식을 하였으며, 두번째 이식은 70일 후 시행하였다. 췌도분리는 변형된 Recordi방법과 비연속성 자당 농도차(discontinuous density gradient)를 이용하였으며 분리한 췌도는 배양 후 환자의 신장기능이 정상화되고 미생물학 검사에서 음성을 확인한 후 경피경간으로 간문맥을 접근하여 16G 폴리 에틸렌 카테터를 이용하여 간실질에 이식하였다. 결과:1차 췌도이식시 순수분리전 췌도수는 210,000개, 순수분리후 획득한 췌도수는 90,000개, 순도 95%, 세포양 1.0mL 이었고, 2차 췌도이식시 순수분리전 췌도수는 420,000개, 순수분리후 획득한 췌도수는 370,000개, 순도 95%, 세포양 1.5mL 이었다. 1,2차 모두 간문맥을 통해 약 20분에 걸쳐 간실질에 주입하였다. 췌도이식전 인슐린요구량은 75∼75U/일, HbA1e 8∼10%, C­펩타이드 0.6ng/mL 였으며, 1차 췌도이식수 7일째 인슐린 요구량은 40U/일, C­펩타이드 1.5ng/mL, FPS 109mg/mL 였고, 40일 추적관찰 후 인슐린 요구량이 36U/일, C­펩타이드 1.8ng/mL, HbA1e 6.5∼7.0%로 안정되었다. 그러나 이식 50일째부터 인슐린 요구량이 50∼56U/일, C­펩타이드 0.6ng/mL, FPS 130∼200mg/dL로 혈당 조절이 불안정하여 다시 췌도이식을 시행하였다. 2차 이식후 50일이 경과한 현재, 인슐린 요구량은 26U/일, C­펩타이드 1.8ng/mL, FPS 90∼120mg/dL로 다시 안정되었다. 결론:췌도이식은 반복이식이 가능하며, 본 환자의 경우 인슐린요구량 감소, 혈당의 안정화 및 C­펩타이드가 상승하여 이식한 췌도의 기능을 확인할 수 있었고, 향후 스테로이드 등 면역억제가 유지 용량으로 감량되면 인슐린 요구량은 더 감소될 것으로 기대된다. Over the past 20 years, significant advances have been made in human islet transplantation. However, cases of prolonged insulin independence after islet allotransplantation have rarely been reported and over time, a slight, gradual decrease in insulin secretion appears to occur, as suggested by the lower C-peptide. Although preliminary clinical success achieved over the past few years has been considerably higher with whole pancreatic transplant than with isolated islet grafts, both approaches remain experimental. Islet grafts might gain, over time, increasing credibility and might eventually provide an easier alternative in terms of grafting procedures and patient management, as compared with the more "traumatizing" whole-pancreas transplantation. Also, using islet, re-transplantation is possible. But it is not known whether re-transplantation of islet could be suitable for those patients who lost grafted islet function. The aim of the present study was to investigate the benefits of re-transplantation of islet in previously simultaneous islets-kidney transplant (SIK) patient who have lost graft function. Methods : The recipient was a 32 year old male. First islet transplantation was underwent at December 25, 1999. However, the grafted islets lost function after 70 days. So we performed re-transplantation of islets. The isolation of islet was conducted sterilely on a laminarflow hood and isolated by a modified Recordimethod. The islet was injected slowly into the liver via a cannular placed in the potalvein for 20 minutes. Results : Transplanted islets were 90,000 IEq at first islet transplantation, 370,000 IEq at second islet transplantation. The insulin requirement was reduced from 75-85 to 35-40 U/day, the basal C-peptide level was 1.5 ng/mL at 7 days posttransplant Unfortunately, the grafted islets lost function after 70 days. After second transplantation, the insulin requirement was reduced to 26 U/day. Conclusions : Despite the continuous need for exogenous insulin therapy, islet transplantation can prevent wide glucose fluctuations, thus resulting in normalization of glycemic control and improvement in HbAlc, and also, show that islets can be successfully and safely re-transplanted intraportally in patients who have lost previously grafted islet function (J Kor Diabetes Asso 457~466, 2000).

닭의 장풀의 수경재배시 적정 pH 및 질소량 분석

양용준,김영식 덕성여자대학교 식물자원연구소 1993 植物資源硏究誌 Vol.1 No.-

본 연구는 닭의장풀의 생육에 미치는 배양액내 NH_4NO_3 농도와 여러 pH값의 효과를 구명함으로써 수경재배시 적합한 배양액내 적정 pH와 질소농도를 밝히는데 목적이 있으며 그 결과는 다음과 같다. 1. 생육중 배양액 pH는 생육전반기 및 배양액 교환초기(2일간)에는 상승하다가 생육후반기 및 배양액 교환 후 (3-5일간)에는 급격히 감소하였다. 2. 재배기간이 경과함에 따라 초장이 신장되고 엽수가 증가하였는데 이러한 경향은 배양액중 처리 질소농도가 높을수록 뚜렷하였다. 3. 생체중과 건물중은 생육중 크게 증가하였으나 pH값(1-6)과 NH_4NO_3 농도에 의해 영향은 인정되지 않았다. 4. Relative growth rate(RGR)는 1M일 때가 6M 보다 약간 높았으며 생육전반기가 후반기보다 높았다 5. NO_3 농도는 매주 처리후 시간이 경과할수록 감소되었는데, 6M의 pH 7에서 잔류량이 가장 높고 1M의 pH 5에서 가장 낮았다. This study was conducted to investigate the effect of NH_4NO_3 concentrations and different solution pHs on growth of Commelina communis and remaining NO_3 contents in nutrient solution. The objects of this research was to reveal the optimum pH value and nitrogen concentration in hydroponic medium. The results were summarized as follows: 1) The solution pH value tended to increase until 2 days after each medium exchange weekly and the first 2 weeks after planting, after then decreased largely. 2) Growth in plant height and development in number of leaves accelerated with adding the hydroponically cultured period and increasing the nitrogen concentration. 3) Fresh and dryweight were markedly influenced by pH values(1 or 6) and NH_4NO_3 concentrations in nutrient solution. 4) Relative growth rate(RGR) showed the highest value at 1M NH_4NO_3 concentrations in nutrient solution, and the RGR between the initial and the 3rd week was more higher than that between the 3rd and 6th week. 5) NO_3 concentration decreased slowly after the change of nutrient solution weekly. The remaining contents were highest at 6M NH_4NO_3 concentrations and pH 7 in nutrient solution.

Synthesis and Anion Binding Properties of the Bridged Urea Derivatives of Calix[4]arene

Yang, Yong-Shik,Ko, Seung-Whan,Song, In-Hee,Ryu, Byung-Ju,Nam, Kye-Chun Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.5

수근관 증후군에서의 무증상성 척골신경병증

문준식,이성수,이지용,한범기,권준범,양현덕,이성익 대한신경과학회 1999 대한신경과학회지 Vol.17 No.2

Differential Expressions of Gap Junction Proteins during Differentiation of Rat Neuronal Stem Cells

Yang, Se-Ran,Cho, Sung-Dae,Ahn, Nam-Shik,Jung, Ji-Won,Park, Joon-Suk,Tiep, Nguyen Ba,Park, Ki-Su,Hong, In-Sun,Jo, Eun-Hye,Seo, Min-Seo,Yoon, Byong-Su,Lee, Yong-Soon,Kang, Kyung-Sun Korean Environmental Mutagen Society 2003 한국환경성돌연변이·발암원학회지 Vol.23 No.1

Gap junctional intercellular communication (GJIC) plays a key role during development, process of tissue differentiation, and in maintenance of adult tissue homeostasis. Neural stem cells leading to formation of cell clusters termed 'neurospheres', can differentiate into neurons, oligodendrocytes, and astrocytes. We investigated the expression levels and distribution of connexin43 (Cx43) and connexin32 (Cx32), abundant gap junctional protein in neural cells and in neurospheres isolated from rat fetus embryonic day (ED) 17. During differentiation of neurospheres, expression of Cx43 and 32 were increased time-dependently within 72 h, and then decreased at 7 day in western blot analysis. TPA-induced inhibition of GJIC was confirmed by decreased fluorescence by SL/DT assay, and induced hyperphosphorylation of Cx43 while no changes in Cx32 levels in western blot assay. Our results indicate that GJIC may be a crucial role in the differentiation of neuronal stem cell. And this GJIC can be inhibited by TPA through the hyperphosphorylation of Cx43.

Staphylococcus epidermidis에 의한 Biofilm 형성에 미치는 Vancomycin, Roxithromycin 및 Rifampin의 효과

임용성,김양리,최정현,유진홍,신완식,강문원 대한감염학회 1999 감염 Vol.31 No.4

방사선 측정 및 해석 연구 : 원자로 냉각수중의 방사능해석에 의한 결함핵연료봉의 평가 Assessment of Defected Fuel by Analysis of Reactor Coolant Activities

양재춘,오희필,전재식,이호연,오헌진,정문규,박해용 충남대학교 기초과학연구소 1987 연구논문집 Vol.7 No.-

중성자와 우라늄의 핵반응에 의해 생성된 핵분열생성물의 물리적 특성을 이용하여 원자로 내의 핵연료 상태를 해석하는 모델을 개선하였다. 이 모델에서는 고체 핵연료 내에서 특정핵종의 핵분열생성물의 생성과 이것이 원자로 냉각재까지 방출되는 과정을 계산하고 추적하여 방사능농도와 결함 핵연료봉의 수를 관계짓는 방정식의 계수들을 결정한다. 핵분열생성물의 거동은 이탈(knock out)과 이동(migration) 두 부분으로 나누어 해석하였으며 트램프 우라늄의 영향을 분리할 수 있도록 하였다. 실측자료로는 가압 경수형 원자로인 고리 원자력발전소 1호기의 1차 냉각재를 분석해서 얻은 I-131과 I-133의 방사능 강도를 이용하였다. 이 실험자료와 위 방정식에서 구한 방사능 강도로부터 구한 결함 핵연료의 수는 제3주기에서 9.34±1.13개 제6주기에서 0.294±0.092개로 나타났다. An improved mothod of assessing fuel status by analyzsis of the fission product in the reactor coolant system is proposed. The release mechanism of specific fission products is established for determination of the coefficients in the equations which relate the radioactivities with the amount of detected fuel. Knock-out and migration models are employed in the formulation of the release mechanism. The influence of the tramp uranium is quantified. Sample calculations were made for KNU 1 reactor system using the I-131 and I-133 concentrations in the primary coolant. The estimated number of defected fuel pins in the third and sixth cycles appeared to be 9.34±1.13 and 0.294±0.092, respectively.

Offsprings Produced by Transcervically Inseminating Frozen-thawed Semen into Uterus of a Estrus-induced Saanen Goat during Non-breeding Season

Yong, Hwan-Yul,Kim, Min-Ah,Bae, Bok-Soo,Kim, Seung-Dong,Jo, Shin-Il,Lim, Yang-Mook,Yoo, Mi-Hyun,Ha, Yong-Hee,Oh, Chang-Shik,Kim, Doo-Hee 韓國受精卵移植學會 2010 한국동물생명공학회지 Vol.25 No.2

We report herein the successful results of estrus induction, sperm cryopreservation and kids born by transcervical insemination of frozen-thawed semen in a Saanen goat. Flugestone acetate (FGA: 60 mg) was inserted into vagina for 15 days. The goat was intramuscularly injected with 400 IU PMSG and 200 IU hCG (: Intervet, Korea) a day before withdrawal of the FGA sponge. Follicles and corpora lutea were identified on both ovaries by laparoscopy. Artificial insemination was performed 46 hours after removal of FGA sponge. The concentration of frozen-thawed semen was and 0.5 ml of frozen-thawed semen was transcervically inseminated into uterine body under anesthesia. Three kids, all females, were born 144 days after artificial insemination. This is the first report producing kids by transcervical insemination of frozen-thawed semen in a Saanen goat of which the estrus was induced by FGA sponges, PMSG and hCG during non-breeding season in Korea.

Evaluation of Nonleaky C.B-17 SCID Mice by ELISA

Yang-Kyu Choi(최양규),Jae-Yoon Choi(최재윤),Eui-Hyun Kown(권의현),Yong-Joon Kim(김용준),Chul-Ho Lee(이철호),Kyu-Shik Jeong(정규식),Byung-Hwa Hyun(현병화) 한국실험동물학회 1996 Laboratory Animal Research Vol.12 No.1

Expression of PDGF-C and PDGF α-receptor in mouse embryos during embryogenesis

Byung Yong Park,In Shik Kim,Hong Hyun Yang,Gou Young Koh,Byung Keon Park 대한체질인류학회 2001 대한체질인류학회 학술대회 연제 초록 Vol.44 No.-