Effects of Alpha lipoic acid (ALA) on non-specific immune responses of nile tilapia, Oreochromis niloticus

Yo-Sep Hwang,Chang-Ju Kim,Su-Hwan Park,Sang-Hoon Choi 한국수산과학회 양식분과 2014 한국수산과학회 양식분과 학술대회 Vol.2014 No.7

Growth and Quality of Soybean Sprouts Affected by Chitosan Treatment and Watering Time

( Yo Sep Kim ),( Na Young Park ),( Hong Kyoon No ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.3

The effects of chitosan concentrations (0.05 and 0.1%) and watering times (1 and 5 min/every 20 min) on growth and selected quality of soybean sprouts were evaluated. In chitosan concentration studies, soaking of soybeans in 0.05% chitosan solution was more effective in increasing total weight of soybean sprouts than soaking in 0.1% chitosan solution. In chitosan treatment and watering time studies, soybeans were soaked in water (W) or 0.05% chitosan solution (CH) for 8 h and then cultivated by spraying water for 1 min (W-1 and CH-1) or 5 min (W-5 and CH-5) for 3 days at 20±2oC. Increase in watering time from 1 min to 5 min increased total weight of soybean sprouts (W-1 vs. W-5 and CH-1 vs. CH-5). At the same watering time, chitosan treatment was more effective in increasing total weight than water treatment (W-1 vs. CH-1 and W-5 vs. CH-5). No significant differences in hardness, mineral content, and DPPH radical scavenging activity were observed among four treatment groups. However, the moisture content increased with increasing watering time. Total free amino acid contents of W-5, CH-1, and CH-5soybean sprouts were all comparable, but higher than that of W-1 soybean sprouts. Increased shelf life of CH-5 soybean sprouts, compared with W-5, was not observed during storage at 10°C for 21 days.

The dietary effect of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus

( Yo-sep Hwang ),( Seok Jin Bang ),( Tae Yun Kang ),( Jae Hyeok Choi ),( Sang Mok Jung ),( In Sung Kang ),( Se Young Jeon ),( Kwan Ha Park ),( Sanghoon Choi ) 한국어병학회 2019 한국어병학회지 Vol.32 No.1

The study investigated the dietary effects of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus. Tilapia were divided in four groups. The first is a fish group fed a basal diet added with 40% medicinal herbs extract (MHE). The second is a fish group fed a basal diet supplied with 2×10⁸ CFU/g of 2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp, respectively (PB). The third group was fed with a mixture of probiotics (2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp) with the medicinal herbs extract added in basal diet (MHE+PB). The fourth group was fed only a basal diet (C). In a non-specific immune parameters analysis, respiratory burst activity, lysozyme activity, phagocytic activity (PA), alternative complement pathway activity (ACH₅₀) and superoxide dismutase (SOD) activity were significantly (p<0.05) increased in the group MHE+PB compared to other groups. Both PB and MHE groups showed a significant (p<0.05) increase in respiratory burst activity, lysozyme activity compared to the control C group, whereas no significant differences were observed in PA, ACH₅₀ and SOD activity compared to the control group. In challenging test, fish were administered with Edwardsiella tarda (E. tarda) on 30 days after feeding with each experimental diet and viable E. tarda cell reduction was checked over 21 days post injection. MHE+PB group showed a significantly (p< 0.05) reduced E. tarda cells compared to other groups. No significant antibacterial difference (p >0.05) was observed between PB and MHE only treated group. Compared to the control, a significant antibacterial difference (p< 0.05) appeared in PB but not in MHE (p >0.05). The results suggest that the probiotics and MHE mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.

Soaking of Soybean Seeds in Milled Chitosan Solution and Its Effect on Growth and Quality of Soybean Sprouts

( Yo Sep Kim ),( Na Young Park ),( Shin Ho Lee ),( Kwang Sup Youn ),( Joon Heon Hong ),( Chang Su Park ),( Hong Kyoon No ) 한국키틴키토산학회 2015 한국키틴키토산학회지 Vol.20 No.1

The effects of milled chitosan solutions on growth and selected quality of soybean sprouts were evaluated.Chitosan solution (1% in 1% acetic acid) was milled for 0, 0.5, 1 and 2 h, and diluted with distilled water to 0.05% to use as soaking solutions. Soybean seeds were soaked in water (control), 0.05% acetic acid and milled chitosan solutions for 8 h, and then cultivated for 3 days at 25 o C. Results indicated that chitosan treatment increased total weight by 7.8-10.0% and hypocotyl length by 6.2-11.1% compared with the control and that the effectiveness of chitosan treatment was not affected by milling times of chitosan solution. Chitosan treatment, irrespective of milling times, did not affect the quality of soybean sprouts. No significant differences in moisture content, hardness and DPPH radical scavenging activity were observed among six treatment groups. The total free amino acid contents of soybean sprouts treated with 0.5 and 1 h-milled chitosan solutions were comparable to that of the control soybean sprouts.

Effects of Drawing Strain and Annealing on the Mechanical Properties for Heavily Cold Drawn Hyper-Eutectoid Steel Wires

Yang, Yo Sep,Bae, Jong Gu,Park, Chan Gyung Trans Tech Publications, Ltd. 2007 Materials science forum Vol.539 No.-

<P>In this study, the effects of drawing strain and annealing at low temperature on the mechanical properties have been investigated. The mechanical properties, such as hardness and strength and fatigue, were performed. The fatigue tests of hyper eutectoid steel wires were performed by the Hunter-type tester, specially designed for ultra thin wires (<0.2mmφ), under the bending stress. The mechanical properties of the steel wires increased with increase of annealing temperature, and the increase was more significant with higher drawing strain. Also, as annealing temperature increased, the hardness and tensile strength gradually increased up to 200 °C and then dramatically decreased at above temperature. However, bending fatigue limit, including fatigue life, showed the some different phenomena with increase of annealing temperature. These different behaviors depending on annealing temperature were discussed in terms of microstructural parameter changes such as pearlite morphology, rearrangement and coalescence of the dislocations.</P>

The dietary effect of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus

Hwang, Yo-Sep,Bang, Seok Jin,Kang, Tae Yun,Choi, Jae Hyeok,Jung, Sang Mok,Kang, In Sung,Jeon, Se young,Park, Kwan Ha,Choi, Sanghoon The Korean Society of Fish Pathology 2019 한국어병학회지 Vol.32 No.1

The study investigated the dietary effects of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus. Tilapia were divided in four groups. The first is a fish group fed a basal diet added with 40% medicinal herbs extract (MHE). The second is a fish group fed a basal diet supplied with $2{\times}10^8CFU/g$ of 2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp, respectively (PB). The third group was fed with a mixture of probiotics (2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp) with the medicinal herbs extract added in basal diet (MHE+PB). The fourth group was fed only a basal diet (C). In a non-specific immune parameters analysis, respiratory burst activity, lysozyme activity, phagocytic activity (PA), alternative complement pathway activity ($ACH_{50}$) and superoxide dismutase (SOD) activity were significantly (p<0.05) increased in the group MHE+PB compared to other groups. Both PB and MHE groups showed a significant (p<0.05) increase in respiratory burst activity, lysozyme activity compared to the control C group, whereas no significant differences were observed in PA, $ACH_{50}$ and SOD activity compared to the control group. In challenging test, fish were administered with Edwardsiella tarda (E. tarda) on 30 days after feeding with each experimental diet and viable E. tarda cell reduction was checked over 21 days post injection. MHE+PB group showed a significantly (p<0.05) reduced E. tarda cells compared to other groups. No significant antibacterial difference (p>0.05) was observed between PB and MHE only treated group. Compared to the control, a significant antibacterial difference (p<0.05) appeared in PB but not in MHE (p>0.05). The results suggest that the probiotics and MHE mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.

Growth and characterization of nitrogen-doped single-walled carbon nanotubes by water-plasma chemical vapour deposition

Min, Yo-Sep,Bae, Eun Ju,Asanov, Igor P,Kim, Un Jeong,Park, Wanjun IOP Pub 2007 Nanotechnology Vol.18 No.28

<P>Nitrogen-doped single-walled carbon nanotubes (N-SWNTs) are directly grown on SiO<SUB>2</SUB>/Si substrates at 450 °C with methane and ammonia gases by water-plasma chemical vapour deposition. The strongest radial breathing mode peak in Raman spectra of the grown N-SWNTs, probed with a 633 nm laser excitation, was assigned to (7, 5) semiconducting nanotubes with a diameter of 0.83 nm. As the doped nitrogen content increases, the D-band to G-band ratio in Raman spectra, indicating the imperfection of nanotubes, gradually increases and saturates at around 4%. X-ray photoelectron spectroscopy shows that nitrogen atoms are doped with a pyridine-like configuration in the N-SWNTs.</P>

벨기에 신앙고백의 역사적 배경과 개혁주의 교회론의 특징 연구

김요섭 ( Yo Sep Kim ) 개혁신학회 2013 개혁논총 Vol.25 No.-

1561년 저지대 지방에서 드 브레가 저술한 벨기에 신앙고백(Confessio Belgica)은 개혁주의 교회론을 이해할 수 있는 중요한 자료 가운데 하나이다. 본 연구는 벨기에 신앙고백 가운데 교회론을 다루는 장들의 내용을 그 역사적 배경과 함께 분석하여 이 신앙고백이 보여주는 개혁주의의 일관된 교회 이해와 더불어 이 신앙고백이 갖는 발전적 특징들을 드러내는 데 그 목적이 있다. 이를 위해 먼저 벨기에 신앙고백에 저술된 16세기 저지대 지방 개혁주의 교회의 상황과 저자인 드 브레의 삶과 신학적 배경, 그리고 이 신앙고백의 직접적 자료들을 검토한다. 이어 벨기에 신앙고백의 28장부터 32장까지 나타난 교회의 정의, 교회의 표지, 교회의 제도라는 세 가지 주제를 갈리아 신앙고백과 베자의 신앙고백의 관련 조항들을 비교하여 이 신앙고백에 나타난 개혁주의 교회론의 일관성과 발전성을 드러내려고 한다. 삼위일체 하나님의 구원의 은혜로부터 교회를 정의하고, 하나님의 말씀의 바른 선포라는 사명을 중심으로 참 교회의 표지를 이해하며, 그리스도의 머리이심이라는 원리로부터 교회의 제도를 세우려 한 점이 벨기에 신앙고백이 가지고 있는 일관된 개혁주의 교회론의 강조점들이다. 벨기에 신앙고백은 그 발전적인 특징으로서 교회의 정의나 제도를 설명할 때 기존의 자료들을 사용하면서도 체계적으로 발전시킨 신학적 진술을 사용한다. 또 권징의 시행을 참 교회의 표지에 명시하여 그리스도의 통치를 교회 가운데 실질적으로 실현하고자 하는 개혁주의의 적극적인 교회론을 보여준다. 벨기에 신앙고백의 교회론이 보여주는 이와 같은 개혁주의적 일관성과 발전적 특징들은 새로운 시대적 과제를 맞이하면서 개혁주의 교회론의 가치를 지키고자 하는 한국 개혁주의교회가 기억해야 할 가치 있는 역사적 유산이다. The Belgic Confession, which was written by Guido de Bres in 1561, has been one of the important sources to understand the ecclesiology of the sixteenth-century reformed church in the Low Countries. The purpose of this study is to manifest both consistent reformed ideas of the church and developing characteristics of the Belgic Confession by analysing the ecclesiological articles in this confession together with its historical background. For this purpose, historical situation of the Reformed church in the sixteenth-century Low Countries, de Bres` life and theology, and the direct sources of the Belgic Confession will be reviewed. Then 5 articles from 28 to32 of this confession will be examined in comparison with the Gallican Confession (1559) and Beza`s Confession of Faith (1560). This comparative examination aims to show the consistent and developing characters of the Belgic Confession in three main ecclsiological themes: definition, marks and government of the church. On the one hand, the Belgic Confession maintains consistent reformed ecclesiological foci in these themes. They are to define the church on the basis of the salvation grace of the triune God, to signify the marks of the true church according to its ministry of proper teaching of God`s word, and to establish church government from the theological principle of Christ`s sole headship. On the other hand, the Belgic Confession has developing features in presenting a more theologically systematized articles about definition and government of the church. Furthermore, this confession includes ecclesiastical discipline in three marks of the true church to reflect the positive aspect of the reformed ecclesiology that endeavors to realize Christ`s rule in the church. Such consistent and developing features of the reformed ecclesiology manifested in the Belgic Confession are both important heritage from which the Korea reformed church can learn how to face various challenges in this time while keep the fundamental principles of the reformed idea of the church.

칼빈의 성찬론의 신학적 요점과 교회론적 의의

김요섭(Yo Sep Kim) 신학지남사 2013 신학지남 Vol.80 No.3

참 경건에 대한 칼빈의 이해와 실천

김요섭(Yo Sep Kim) 고신대학교 개혁주의학술원 2020 갱신과 부흥 Vol.25 No.-

칼빈은 종교개혁자로서 중세 말 로마 가톨릭의 종교 체계를 불경건이라고 비판하고 성경에 근거한 기독교의 참 경건을 회복하기 위해 노력했다. 본 논문의 목적은 칼빈의 참 경건의 이해와 그 실현을 위한 시도 모두가 공동체적인 측면에서 제시되었으며 율법의 규범적 기준을 따라 추구되었음을 증명하는 것이다. 이를 위해 칼빈이 그가 견지한 적극적이며 실천적인 율법의 제3용도에 대한 이해로부터 참된 경건의 개념을 주장했을 뿐 아니라 율법의 규범을 일반적 기준으로 삼아 경건의 공동체적 구현을 추구했음을 논증할 것이다. 이를 위하여 가장 먼저 칼빈이 당시 로마 가톨릭의 불경건을 비판하면서 제시한 기독교 신앙의 참 경건의 개념과 그 원리를 검토할 것이다. 그 후 참 경건의 표현의 현장인 예배와 경건의 훈련의 장인 그리스도인의 공동체적 삶에 대해 칼빈이 어떤 실천적 제안을 제시했는지 검토할 것이다. 결론에서는 칼빈의 경건이해와 실천의 노력이 오늘날 교회를 위해 가질 수 있는 현대적 의의를 간략하게 제안할 것이다. 칼빈은 로마 가톨릭이 만들어 놓은 외형중심의경건 체계의 오류를 비판하고 참 경건은 하나님의 사랑에 대한 깨달음에 의한 내면적 변화에서 시작되어야 한다고 말했다. 그러나 칼빈은 참 경건이 공동체적 실천으로 나타나야 하며 율법이 대표하는 성경의 가르침에 따라 지속적으로 훈련되어야 한다고 강조했다. 21세기 한국 교회의 상황 속에서도 칼빈의 경건 이해와 그 실천을 위한 시도들은 의미 있는 역사적 교훈을 던져준다. 특히 그가 강조한 참 경건의 공동체적인 성격과, 경건의 구체적 실천에 있어 율법의 가르침을 신적 규범으로 삼은 해석은 특정한 시대와 지역을 차이를 넘어서 성경의 권위를 존중하는 모든 기독교 공동체에게 보편적이며 유효한 경건 추구의 교훈을 제공한다. As a protestant reformer, John Calvin criticized the Roman Catholic religious system as impiety, and endeavored to recover the true piety from the biblical point of view. This paper aims to argue that Calvin’s idea of true piety and his practical proposal of piety are presented with the universal and definitive standard of God’s Words for the Christian community. In particular, it is argued that Calvin’s positive and covenantal interpretation of the third use of the law is an essential foundation upon which he suggested the right meaning of true piety and pursued its realization in Christian life. For this purpose, this study reviews Calvin’s idea of true piety and its principles, which are contrasted with wrong teaching and practice of the Roman Catholic impiety. After this review, this study deals with Calvin’s suggestions of reforming worship service and Christian life, For Calvin, worship service is the expressive field of true piety, and Christian life is the disciplinal field of piety. In conclusion, significance of Calvin’s idea and practice of true piety for the Christian communities today is briefly mentioned. Criticizing the errors of the Roman Catholic rituals and teachings of Christian life, Calvin consistently argued that true piety had to start with the inner change which arises from the recognition of God’s sincere love. However, he never forget to emphasize that true piety should be manifested in the communal dimension and continuously exercised faithful to the God’s commandment. His emphasis on the communal character of piety, and the interpretation of the law as the divine standard of practicing piety provide a general and effective lesson for every Christian community which has a high sense of the authority of scripture.