Collapse risk evaluation method on Bayesian network prediction model and engineering application

WANG, Jing,LI, Shucai,LI, Liping,SHI, Shaoshuai,XU, Zhenhao,LIN, Peng Techno-Press 2017 Advances in computational design Vol.2 No.2

Collapse was one of the typical common geological hazards during the construction of tunnels. The risk assessment of collapse was an effective way to ensure the safety of tunnels. We established a prediction model of collapse based on Bayesian Network. 76 large or medium collapses in China were analyzed. The variable set and range of the model were determined according to the statistics. A collapse prediction software was developed and its veracity was also evaluated. At last the software was used to predict tunnel collapses. It effectively evaded the disaster. Establishing the platform can be subsequent perfect. The platform can also be applied to the risk assessment of other tunnel engineering.

Grouting effects evaluation of water-rich faults and its engineering application in Qingdao Jiaozhou Bay Subsea Tunnel, China

Zhang, Jian,Li, Shucai,Li, Liping,Zhang, Qianqing,Xu, Zhenhao,Wu, Jing,He, Peng Techno-Press 2017 Geomechanics & engineering Vol.12 No.1

In order to evaluate the grouting effects of water-rich fault in tunnels systematically, a feasible and scientific method is introduced based on the extension theory. First, eight main influencing factors are chosen as evaluation indexes by analyzing the changes of permeability, mechanical properties and deformation of surrounding rocks. The model of evaluating grouting effects based on the extension theory is established following this. According to four quality grades of grouting effects, normalization of evaluation indexes is carried out, aiming to meet the requirement of extension theory on data format. The index weight is allocated by adopting the entropy method. Finally, the model is applied to the grouting effects evaluation in water-rich fault F4-4 of Qingdao Jiaozhou Bay Subsea Tunnel, China. The evaluation results are in good agreement with the test results on the site, which shows that the evaluation model is feasible in this field, providing a powerful tool for systematically evaluating the grouting effects of water-rich fault in tunnels.

Preparation of epoxypropyl functionalized graphene oxide and its anticorrosion properties complexed with epoxy resin

Xiaofeng Yang,Yuepeng Zhang,Zhiping Chen,Yinshuai Yang,Hongxia Jing,Zhenhao Sun,Huijie Wang 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.12

Functionalized graphene oxide (GO)/epoxy resin (EP) composite coating has attracted attention due to its advantages of epoxy resin in acid resistance, anticorrosion and having the characteristics of GO such as good chemical stability and strong electrolyte barrier properties. In this study, epoxypropyl functionalized graphene oxide (EFGO) was synthesized with a new strategy by grafting epichlorohydrin (ECH) onto the surface of GO. EFGO was then crosslinked with EP by diethylenetriamine (DETA) to fabricate EFGO/EP anticorrosive coating. The structure and property of EFGO and EFGO/EP were characterized and evaluated by various methods such as FTIR, Raman, XPS, SEM, and EIS. The results showed that the graft amount of epoxypropyl on GO surface could be effectively increased by using KI-KF catalysis system. The increase of the graft amount further improved the compatibility between EFGO and EP. EFGO/EP had a network structure with EFGO as the “central node”. The “central node” locked the movement of EP chain segment, as improving the thermal stability of EFGO/EP. The EFGO/EP coating fabricated by the EFGO, which grafted larger epoxypropyl, had good anticorrosion performance and adhesion with steel plates, showing a good barrier effect on electrolyte migration. The coating resistance reached up 4.88×108 cm2.

MiRNA320a Inhibitor-Loaded PLGA-PLL-PEG Nanoparticles Contribute to Bone Regeneration in Trauma-Induced Osteonecrosis Model of the Femoral Head

Zhang Ying,Li Chuan,Wei Qiushi,Yuan Qiang,He Wei,Zhang Ning,Dong Yiping,Jing Zhenhao,Zhang Leilei,Wang Haibin,Cao Xiangyang 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1

BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH. BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH.

The characteristic of the synonymous codon usage and phylogenetic analysis of hepatitis B virus

Xiaoming Qi,Chaojun Wei,Yonghong Li,Yu Wu,Hui Xu,Rui Guo,Yanjuan Jia,Zhenhao Li,Zhenhong Wei,Wanxia Wang,Jing Jia,Yuanting Li,Anqi Wang,Xiaoling Gao 한국유전학회 2020 Genes & Genomics Vol.42 No.7

Background Hepatitis B virus (HBV) infection is a crucial medical issue worldwide. The dependence of HBV replication on host cell machineries and their co-evolutionary interactions prompt the codon usage pattern of viral genes to translation selection and mutation pressure. Objective The evolutionary characteristics of HBV and the natural selection effects of the human genome on the codon usage characteristics were analyzed to provide a basis for medication development for HBV infection. Methods The codon usage pattern of sequences from different HBV genotypes of our isolates and reference HBV genome sequences downloaded from the National Center for Biotechnology Information (NCBI) database were analyzed by computing the relative synonymous codon usage (RSCU), nucleotide content, codon adaptation index (CAI) and the effective number of codons (ENC). Results The highest ENC values were observed in the C genotypes, followed by the B genotypes. The ENC values indicated a weak codon usage bias (CUB) in HBV genome. The number of codons differentially used between the three genotypes was markedly higher than that of similarly used codons. High CAI values indicated a good adaptability of HBV to its host. The ENC plot indicated the occurrence of mutational pressure in the three genotypes. The mean Ka/Ks ratios in the three genotypes were lower than 1, which indicated a negative selection pressure. The CAI and GC3% plot indicated the existence of CUB in the HBV genome. Conclusions Nucleotide composition, mutation bias, negative selection and mutational pressure are key factors influencing the CUB and phylogenetic diversity in HBV genotypes. The data provided here could be useful for developing drugs for HBV infection.