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( Yue Lu ),( Seok Jong Suh ),( Xian Li ),( Seung Lark Hwang ),( Ying Li ),( Kyoung Hwang Bo ),( Soon Jin Park ),( Hyeun Wook Chang ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0
In this study, we examined the effects of citreorosein (CIT), an anthraquinone component of Polygoni cuspidati radix (P. cuspidati, Polygonaceae), on cyclooxygenase (COX)-2 dependent prostaglandin (PG)D2 generation in mast cells, central effector cells of allergy and other inflammatory diseases. CIT strongly inhibited COX-2-dependent PGD2 generation in a concentration-dependent manner in mouse bone marrow-derived mast cells (BMMCs) stimulated with stem cell factor (SCF)/IL-10/LPS. In an effort to identify the mechanisms underlying the inhibition of COX-2-dependent PGD2 generation by CIT, we examined the effects of this compound on MAP kinases, Akt and NF-κB signaling pathways, which are essential for COX-2 induction. CIT inhibited nuclear translocation of the nuclear factor (NF)-κB p65 subunit and its cognate DNA-binding activity, which correlated with its inhibitory effects on the phosphorylation of Akt and IKK and subsequent phosphorylation and degradation of IκB. Furthermore, CIT significantly attenuated the DNA binding of activator protein (AP)-1 that regulates COX-2 expression through the reduction of the phosphorylation of c-Jun. Moreover, inhibition of PGD2 generation by CIT was accompanied by a decrease in phosphorylation of cytosolic phospholipase A2α. Taken together, the present study suggests that CIT represents a potential therapeutic approach for the treatment of inflammatory diseases.
( Yue Lu ),( Seok Jong Suh ),( Choong Hwan Kwak ),( Kyung Min Kwon ),( Chang Seob Seo ),( Ying Li ),( Ye Jin ),( Xian Li ),( Seung Lark Hwang ),( Ok Yun Kwon ),( Young Chae Chang ),( Young Guk Park ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0
Saucerneol F (SF), a new tetrahydrofuran-type sesquilignan isolated from Saururus chinensis, dose-dependently inhibited nitric oxide (NO) production, with concomitant reduction of inducible nitric oxide synthase (iNOS) protein and mRNA expression in lipopolysaccharide (LPS)-stimulated murine macrophage RAW264.7 cells. To elucidate the molecular mechanism underlying the inhibition of iNOSexpression by SF, we assessed the effects of SF on nuclear factor-κB (NF-κB) DNA-binding activity,NF-κB-dependent reporter gene activity, inhibitory factor-κB (IκB) phosphorylation and degradation, and p65 nuclear translocation. Treatment with SF decreased the luciferase activities of NF-κB reporter promoters in a dose-dependent manner and translocation of NF-κB p65. In addition, pretreatment of SF reduced LPS-stimulated activation of mitogen-activated protein kinases (MAPKs) including extracellular signal-regulated kinase 1/2 (ERK1/2), p38 MAPK, and c-Jun NH(2)-terminal kinase (JNK). Furthermore, SF attenuated the luciferase activities of AP-1 reporter promoters and the DNA-binding capacity of AP-1. Taken together, the present results indicate that SF attenuates NO production andiNOS expression by blocking LPS-induced activation of NF-κB, MAPKs, and AP-1, suggesting that SF is potentially applicable as an anti-inflammatory drug.ⓒ2011 Elsevier B.V.All rights reserved.
( Yue Lu ),( Ying Li ),( Chang Seob Seo ),( Makoto Murakami ),( Jong Keun Son ),( Hyeun Wook Chang ) 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Previously we reported that saucerneol D (SD), a naturally occurring sesquilignan isolated from Saururus chinensis (S. chinensis) suppressed lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. The aim of this study was to elucidate whether SD modulates the generation of other inflammatory mediators in activated mast cells. We investigated the effects of SD on cyclooxygenase-2 (COX-2)-dependent prostaglandin D(2) (PGD(2)) and 5-lipoxygenase (5-LO)-dependent leukotriene C(4) (LTC(4)) generations as well as degranulation in cytokine-stimulated mouse bone marrow-derived mast cells (BMMCs). Biochemical analyses of the cytokine-mediated signaling pathways showed that SD suppressed the phosphorylation of Syk kinase and multiple downstream signaling processes including phospholipase Cγ1 (PLCγ1)-mediated intracellular Ca(2+) influx and activation of mitogen-activated protein kinases (MAPKs; including extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun NH(2)-terminal kinase (JNK) and p38) and the nuclear factor-κB (NF-κB) pathway. Taken together, the present study suggests that SD suppresses eicosanoid generation and degranulation through Syk-dependent pathway in BMMCs. Crown Copyrighr ⓓ2012 Published by Elsevier Ltd, All rights reserved.
( Yue Lu ),( Ying Li ),( Yurn Dong Jahng ),( Jong Keun Son ),( Hyeun Wook Chang ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0
The aim of this study was to evaluate whether citreorosein (CIT), a naturally occurring anthraquinone isolated from Polygoni cuspidati (P. cuspidati) radix, modulates degranulation and 5-lipoxygenase (5-LO)-dependent leukotriene C(4) (LTC(4)) generation in mast cells. Cit suppresses both degranulationand the generation of LTC(4) in a dose-dependent manner in stem cell factor (SCF)-mediated mouse bone marrow-derived mast cells (BMMCs). With regard to its molecular mechanism of action, we investigated the effects of CIT on intracellular signaling and mast cell activation employing BMMCs. Binding of SCF to c-Kit on mast cell membranes induced increases in intrinsic tyrosine kinase Syk activity and activation of multiple downstream events including phosphorylation of phospholipase Cγ (PLCγ), mobilization of intracellular Ca(2+), phosphatidylinositol 3-kinase (PI3K), Akt, MAP kinases (MAPKs), translocation of phospho-phospholipase A(2) (PLA(2)) and 5-LO. The results from the biochemical analysis demonstrate that CIT attenuates degranulation and LTC(4) generation through the suppression of multiple step signaling and would be beneficial for the prevention of allergic inflammation.
( Yue Lu ),( Ying Li ),( Mei Hua Jin ),( Ju Hye Yang ),( Xian Li ),( Guang Hsuan Chao ),( Hyo Hyun Park ),( Young Na Park ),( Jong Keun Son ),( Eun Kyung Lee ),( Hyeun Wook Chang ) 영남대학교 약품개발연구소 2012 영남대학교 약품개발연구소 연구업적집 Vol.22 No.0
ETHNOPHARMACOLOGICAL RELEVANCE: The flowers of Inula japonica (Inulae Flos) have long been used in traditional medicine for the treatment of bronchitis, digestive disorders, and inflammation. However, the mechanisms underlying its anti-inflammatory effects remain yet to be elucidated. The objectives of this study were 1) to assess the anti-allergic activity of the ethanol extract of flowers of Inula japonica extract (IFE) in vivo, 2) to investigate the mechanism of its action on mast cells in vitro, and 3) to identify its major phytochemical compositions. MATERIALS AND METHODS: The anti-allergic activity of IFE was evaluated using mouse bone marrow-derived mast cells (BMMCs) in vitro and a passive cutaneous anaphylaxis (PCA) animal model in vivo. The effects of IFE on mast cell activation were evaluated in terms of degranulation, eicosanoid generation, Ca(2+) influx, and immunoblotting of various signaling molecules. RESULTS: IFE inhibited degranulation and the generation of eicosanoids (PGD(2) and LTC(4)) in stem cell factor (SCF)-stimulated BMMCs. Biochemical analysis of the SCF-mediated signaling pathways demonstrated that IFE inhibited the activation of multiple downstream signaling processes including mobilization of intracellular Ca(2+) and phosphorylation of the mitogen-activated protein kinases (MAPKs), PLCγ1, and cPLA(2) pathways. When administered orally, IFE attenuated themast cell-mediated PCA reaction in IgE-sensitized mice. Its major phytochemical composition included three sesquiterpenes, 1-O-acetylbritannilactone, britanin and tomentosin. CONCLUSIONS: This study suggests that IFE modulates eicosanoids generation and degranulation through the suppression of SCF-mediated signaling pathways that would be beneficial for the prevention of allergic inflammatory diseases. Anti-allergic activity of IFE may be in part attributed particularly to the presence of britanin and tomentosin as major components evidenced by a HPLC analysis. Crown Copyright ⓒ2012 Published by Elsevier lreland Ltd. All rights reserved.
Xing Yue Xu,Eun Seob Yi,Chang Ho Kang,Ying Liu,Yeong-Geun Lee,Han Sol Choi,Hyun Bin Jang,Yue Huo,Nam In Baek,Deok Chun Yang,Yeon Ju Kim 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6
Background: Main bioactive constituents and pharmacological functions of ripened red ginseng berry(Panax ginseng Meyer) have been frequently reported. Yet, the research gap targeting the beneficial activitiesof transformed green ginseng berries has not reported elsewhere. Methods: Ginsenosides of new green berry cultivar K-1 (GK-1) were identified by HPLC-QTOF/MS. Ginsenosidesbioconversion in GK-1 by bgp1 enzyme was confirmed with HPLC and TLC. Then, mechanismsof GK-1 and b-glucosidase (bgp1) biotransformed GK-1 (BGK-1) were determined by QuantitativeReverse Transcription-Polymerase Chain Reaction and Western blot. Results: GK-1 possesses highest ginsenosides especially ginsenoside-Re amongst seven ginseng cultivarsincluding (Chunpoong, Huangsuk, Kumpoong, K-1, Honkaejong, Gopoong, and Yunpoong). Ginseng root’sbiomass is not affected with the harvest of GK-1 at 3 weeks after flowering period. Then, Re is bioconvertedinto a promising pharmaceutical effect of Rg2 via bgp1. According to the results of cell assays,BGK-1 shows decrease of tyrosinase and melanin content in a-melanocyte-stimulating hormonechallenged-murine melanoma B16 cells. BGK-1 which is comparatively more effective than GK-1 extractshows significant suppression of the nuclear factor (NF)-kB activation and inflammatory target genes, inLPS-stimulated RAW 264.7 cells. Conclusion: These results reported effective whitening and anti-inflammatory of BGK-1 as compared toGK-1.
Lu, Yue,Li, Ying,Jahng, Yurndong,Son, Jong-Keun,Chang, Hyeun Wook Dr. W. Junk B. V. Publishers ; Kluwer Academic Pub 2012 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.365 No.1
<P>The aim of this study was to evaluate whether citreorosein (CIT), a naturally occurring anthraquinone isolated from Polygoni cuspidati (P. cuspidati) radix, modulates degranulation and 5-lipoxygenase (5-LO)-dependent leukotriene C(4) (LTC(4)) generation in mast cells. Cit suppresses both degranulation and the generation of LTC(4) in a dose-dependent manner in stem cell factor (SCF)-mediated mouse bone marrow-derived mast cells (BMMCs). With regard to its molecular mechanism of action, we investigated the effects of CIT on intracellular signaling and mast cell activation employing BMMCs. Binding of SCF to c-Kit on mast cell membranes induced increases in intrinsic tyrosine kinase Syk activity and activation of multiple downstream events including phosphorylation of phospholipase C관 (PLC관), mobilization of intracellular Ca(2+), phosphatidylinositol 3-kinase (PI3K), Akt, MAP kinases (MAPKs), translocation of phospho-phospholipase A(2) (PLA(2)) and 5-LO. The results from the biochemical analysis demonstrate that CIT attenuates degranulation and LTC(4) generation through the suppression of multiple step signaling and would be beneficial for the prevention of allergic inflammation.</P>
한·중 공포소구 유형에 따른 담배 패키지디자인 경고이미지에 관한 연구
우웨잉(Yue Ying Wu),이창근(Chang Keun Lee) 한국디자인리서치학회 2022 한국디자인리서치 Vol.7 No.4
본 연구는 공포소구의 개념 및 유형을 재정리하였으며 이를 바탕으로 담배 패키지디자인 경고이미지의 유형을제시하였다. 한·중 담배패키지 경고이미지 활용 현황 및 차이점을 분석하기 위해 구체적 한·중 사례 84개를 연구대상으로 유형, 요소, 표현기법 등을 분석하였다. 결론적으로 유형별로는 한·중 모두 개인피해형 경고이미지를 가장 많이 활용하는 것을 확인하였다. 또한, 광고 자극 범위 측면에서 면적을 크게 활용하고 주목성이 높은위치에 제시하고 다양한 표현기법을 응용하는 경고이미지를 투입하는 필요성을 보인다. 광고 자극 주기 측면에서 다양한 내용으로 제시하고 수량이 많고 정기적으로 개정하는 경고이미지를 투입하는 필요성이 대두되고있다. 본 연구의 한계점과 향후연구 제언을 바탕으로 담배 패키지디자인 경고이미지에 대한 다양한 후속 연구들이 진행되어 효과적으로 금연을 위한 정책을 제공할 수 있는 광고를 집행하는데 기여할 수 있기를 기대한다. This study rearranged the concept and type of fear appeal, and suggested the type of warning image of cigarette package design. In order to analyze the current status and differences in the use of cigarette package warning images in Korea and China, 84 specific cases were analyzed for types, elements, and expression techniques. In conclusion, by type, it was confirmed that both Korea and China used the most personal damage warning images. In addition, in terms of the advertising stimulus range, it is necessary to use the area of the warning image to a large extent, present it in a highly noticeable position, and insert a warning image that applies various expression techniques. In terms of the advertising stimulation cycle, the need to present a variety of contents and to input warning images that are regularly revised with a large number is emerging. Based on the limitations of this study and the suggestions for future research, it is hoped that warning images can contribute to the execution of advertisements that can effectively provide a policy for smoking cessation.