Hypolipidemic and Antioxidative Effects of Glossogyne tenuifolia in Hamsters Fed an Atherogenic Diet

Yi-Ning Lee,Guoo-Shyng Wang Hsu,Wan-Teng Lin,Yi-Fa Lu 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.5

Glossogyne tenuifolia (GT) Cassini is a special herbal tea in the Penghu Islands, Taiwan, and has a long history of being used as an antipyretic, detoxifying, and anti-inflammatory remedy in folk medicine among local residents. The aim of this study was to investigate the effect of hot water extracts from GT on oxidative stress and lipid metabolism in animals. Five- to 6-week-old male Syrian hamsters were divided into four groups (n = 14) for different treatments, that is: control group (C), high-fat/cholesterol (HF) group, HF diet containing 0.5% (GT0.5) and 1.5% (GT1.5) GT extracts for 4 weeks. Hamsters fed with 0.5% GT powder as well as 1.5% GT powder exhibited reduced serum total cholesterol (TC), conjugated diene of low-density lipoprotein (LDL), and increased serum antioxidant capacity, but 1.5% GT powder was more potent at loweringserum LDL cholesterol and thiobarbituric acid reactive substance concentrations than 0.5% GT. GT extracts significantly lowered liver triacylglycerol (TG) concentration by diminishing activities of fatty acid synthase (FAS) and glucose-6-phosphate dehydrogenase (G-6-PDH). In addition, fecal excretion of cholesterol and bile acids were increased in GT extract groups. In conclusion, GT extracts increase the antioxidative capacity, decrease serum TC, inhibit the activities of FAS and G-6-PDH, and further reduce liver TG accumulation in hamster fed on atherogenic diets.

HCV, Acute, LT : Hepatic Stellate Cells Modulate Functions of Tregs in IFNγ-mediated Hepatitis of Mice

( Qin Ning ),( Young Sun Lee ),( Hyon Seung Yi ),( Hyuk Soo Eun ),( Won Il Jeong ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: Hepatic stellate cells (HSCs) perform important roles not only in liver fibrosis but also in inflammation by regulating activation or induction of immune cells and producing various chemokines and cytokines. Moreover, it has been reported that HSCs are also involved in the induction of regulatory T cells (Tregs). Therefore, we investigated the immune regulatory role of HSCs to Tregs in interferon γ (IFNγ)- mediated hepatitis of mice. Methods: To induce IFNγ-mediated hepatitis in mice, we injected concanavalin A (ConA) to mice through tail vein at dose of 12 μg/g. Mice were sacrificed at 0, 3, 12 and 24 hours after ConA treatment. In vitro experiment, isolated natural Tregs from lymph nodes and spleen were co-cultured with HSCs and then analyzed the FoxP3 expression and gene expressions. Results: After ConA treatment, liver injuries sharply increased a n d p e a k e d a t 2 4 h o u r s a n d t h e p o p u l a t i o n o CD4+CD25+FoxP3+ Tregs was also significantly increased. In addition, isolated HSCs after ConA treatment showed enhanced expressions of TGF-β and IL-10. In vitro co-culturing Tregs with HSCs, HSCs significantly up-regulated Foxp3 expression in Tregs compared to that of non-co-cultured Tregs. Moreover, levels of IL-10 and TGF-β at supernatant remarkably increased in the co-cultured group compared with those of Tregs or HSCs only cultured group. Conclusions: In ConA-induced hepatitis, HSCs might regulate the expression of FoxP3 in Tregs by producing cytokines such as IL-10 and TGF-β. Therefore, the regulation of function in HSCs could be a new therapeutic target for immune-mediated hepatitis.

Acupotomy for scapulohumeral periarthritis

Chang-qing Guo,Shi Ning Ma,Xin Yi Fu,Quan Gui Wang,Mira Lee 대한침구의학회 2020 대한침구의학회지 Vol.37 No.4

Background: Scapulohumeral periarthritis causes pain and stiffness, and limits movement but it is a treatable condition. This was a clinical study of acupotomy treatment for scapulohumeral periarthritis. Methods: There were 80 patients randomly assigned to the traditional Chinese Medicine group (acupotomy) and the Western medicine group (naproxen), with 40 cases in each group. All patients had adjunct physiotherapy exercises for 14 days. Patients received acupotomy treatment 3 times for 14 days (Day 0, 7 and 14) or naproxen (0.22 g capsule; a non-steroidal anti-inflammatory drug) 3 times a day, for 14 days. The visual analogue scale (VAS) scores, range of motion (ROM) values, and the Melle scale, together with the therapeutic standard of diseases and syndromes in traditional Chinese Medicine were used for diagnosis and evaluation. Results: There were significant differences in the VAS scores, ROM, Melle scores, cure rate and total effective rate in the group which took naproxen and the acupotomy group, before and after treatment (p < 0.01). There were significant differences in the changes in VAS, ROM and Melle scores between the 2 groups (p < 0.01), and the acupotomy group was better than the naproxen group. Conclusion: Traditional Chinese Medicine and Western medicine can improve functional activity and reduce the level of pain experienced by patients suffering from scapulohumeral periarthritis. However, improvement of functional activity of the shoulder joint following acupotomy treatment was more obvious than the use of a non-steroidal anti-inflammatory drug, and the cure rate, and total effective rate of acupotomy was better.

Metformin Inhibits Nuclear Receptor TR4–Mediated Hepatic Stearoyl-CoA Desaturase 1 Gene Expression With Altered Insulin Sensitivity

Kim, Eungseok,Liu, Ning-Chun,Yu, I-Chen,Lin, Hung-Yun,Lee, Yi-Fen,Sparks, Janet D.,Chen, Lu-Min,Chang, Chawnshang American Diabetes Association 2011 Diabetes Vol.60 No.5

<P><B>OBJECTIVE</B></P><P>TR4 is a nuclear receptor without clear pathophysiological roles. We investigated the roles of hepatic TR4 in the regulation of lipogenesis and insulin sensitivity in vivo and in vitro.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>TR4 activity and phosphorylation assays were carried out using hepatocytes and various TR4 wild-type and mutant constructs. Liver tissues from TR4 knockout, C57BL/6, and <I>db/db</I> mice were examined to investigate TR4 target gene stearoyl-CoA desaturase (<I>SCD</I>) 1 regulation.</P><P><B>RESULTS</B></P><P>TR4 transactivation is inhibited via phosphorylation by metformin-induced AMP-activated protein kinase (AMPK) at the amino acid serine 351, which results in the suppression of SCD1 gene expression. Additional mechanistic dissection finds TR4-transactivated <I>SCD1</I> promoter activity via direct binding to the TR4-responsive element located at −243 to −255 on the promoter region. The pathophysiological consequences of the metformin→AMPK→TR4→SCD1 pathway are examined via TR4 knockout mice and primary hepatocytes with either knockdown or overexpression of TR4. The results show that the suppression of <I>SCD1</I> via loss of TR4 resulted in reduced fat mass and increased insulin sensitivity with increased β-oxidation and decreased lipogenic gene expression.</P><P><B>CONCLUSIONS</B></P><P>The pathway from metformin→AMPK→TR4→SCD1→insulin sensitivity suggests that TR4 may function as an important modulator to control lipid metabolism, which sheds light on the use of small molecules to modulate TR4 activity as a new alternative approach to battle the metabolic syndrome.</P>

전기적 퓨즈 프로그래밍을 이용한 1T-SRAM 리페어용 리던던시 제어 회로 설계

이재형,전황곤,김광일,김기종,여억녕,하판봉,김영희,Lee, Jae-Hyung,Jeon, Hwang-Gon,Kim, Kwang-Il,Kim, Ki-Jong,Yu, Yi-Ning,Ha, Pan-Bong,Kim, Young-Hee 한국정보통신학회 2010 한국정보통신학회논문지 Vol.14 No.8

본 논문에서는 전기적인 퓨즈 프로그래밍을 이용한 1T-SRAM 리페어용 리던던시 제어 회로를 설계하였다. 공급전원이 낮아지더라도 외부 프로그램 전원을 사용하여 높은 프로그램 파워를 eFuse (electrical fuse)에 공급하면서 셀의 읽기 전류를 줄일 수 있는 듀얼 포트 eFuse 셀을 제안하였다. 그리고 제안된 듀얼 포트 eFuse 셀은 파워-온 읽기 기능으로 eFuse의 프로그램 정보가 D-래치에 자동적으로 저장되도록 설계하였다. 또한 메모리 리페어 주소와 메모리 액세스 주소를 비교하는 주소 비교 회로는 dynamic pseudo NMOS 로직으로 구현하여 기존의 CMOS 로직을 이용한 경우 보다 레이아웃 면적을 19% 정도 줄였다. 전기적인 퓨즈 프로그래밍을 이용한 1T-SRAM 리페어용 리던던시 제어 회로는 동부하이텍 $0.11{\mu}m$ Mixed Signal 공정을 이용하여 설계되었으며, 레이아웃 면적은 $249.02{\times}225.04{\mu}m^{2}$이다. In this paper, we design a redundancy control circuit for 1T-SRAM repair using electrical fuse programming. We propose a dual port eFuse cell to provide high program power to the eFuse and to reduce the read current of the cell by using an external program supply voltage when the supply power is low. The proposed dual port eFuse cell is designed to store its programmed datum into a D-latch automatically in the power-on read mode. The layout area of an address comparison circuit which compares a memory repair address with a memory access address is reduced approximately 19% by using dynamic pseudo NMOS logic instead of CMOS logic. Also, the layout size of the designed redundancy control circuit for 1T-SRAM repair using electrical fuse programming with Dongbu HiTek's $0.11{\mu}m$ mixed signal process is $249.02 {\times}225.04{\mu}m^{2}$.