A Survey of the Geographic Distribution of Ophiocordyceps sinensis

Yi Li,Xiao-Liang Wang,Lei Jiao,Yi Jiang,Hui Li,Si-Ping Jiang,Ngarong Lhosumtseiring,Shen-Zhan Fu,Cai-Hong Dong,Yu Zhan,Yi-Jian Yao 한국미생물학회 2011 The journal of microbiology Vol.49 No.6

Ophiocordyceps sinensis is one of the best known fungi in Traditional Chinese Medicine. Many efforts have been devoted to locating the production areas of this species resulting in various reports; however, its geographic distribution remains incompletely understood. Distribution of O. sinensis at the county level is clarified in this work based on both a literature search and fieldwork. More than 3600 publications related to O. sinensis were investigated, including scientific papers, books, and online information. Herbarium specimens of O. sinensis and field collections made by this research group during the years 2000-2010 were examined to verify the distribution sites. A total of 203 localities for O. sinensis have been found, of which 106 are considered as confirmed distribution sites, 65 as possible distribution sites, 29 as excluded distribution sites and three as suspicious distribution sites. The results show that O. sinensis is confined to the Tibetan Plateau and its surrounding regions, including Tibet, Gansu, Qinghai, Sichuan, and Yunnan provinces in China and in certain areas of the southern flank of the Himalayas, in the countries of Bhutan, India and Nepal, with 3,000 m as the lowest altitude for the distribution. The fungus is distributed from the southernmost site in Yulong Naxi Autonomous County in northwestern Yunnan Province to the northernmost site in the Qilian Mountains in Qilian County, Qinghai Province, and from the east edge of the Tibetan Plateau in Wudu County, Gansu Province to the westernmost site in Uttarakhand, India. The clarification of the geographic distribution of O. sinensis will lay the foundation for conservation and sustainable use of the species.

Weight Gain in Pregnancy, Maternal Age and Gestational Age in Relation to Fetal Macrosomia

( Yi Li ),( Qi-fei Liu ),( Dan Zhang ),( Ying Shen ),( Kui Ye ),( Han-lin Lai ),( Hai-qing Wang ),( Chuan-lai Hu ),( Qi-hong Zhao ),( Li Li ) 한국임상영양학회 2015 Clinical Nutrition Research Vol.4 No.2

To investigate the possible risk factors related to macrosomia. Pregnant women and their newborns (n = 1041) were recruited from a cohort study in Maternal and Child Care Center of Hefei from January 2011 to July 2012. Questionnaires were applied to collect the demographic data besides the medical records. Detailed health records of the entire pregnancy were obtained using retrospective study. Meanwhile the data of neonatal outcomes was prospectively tracked. Associations between exposure risk factors and macrosomia were analyzed using Pearson’s chi squared test. Logistic regression models were used to assess the independent association between these potential predictors and macrosomia. The incidence of macrosomia of this cohort was 11.24% of which male: female = 2.55:1. Male incidence (8.07%) of macrosomia was higher than female (3.17%), p < 0.001. Body mass index (BMI) before pregnancy (pre-BMI), maternal height, parity were not independently associated with macrosomia; multiple logistic regression analysis indicated that macrosomia was mainly independently associated with weight gain in pregnancy (OR=1.14,95% CI [1.10-1.19]), maternal age (OR = 1.09,95% CI [1.03-1.15]) and gestational age (OR = 1.62,95% CI [1.31-1.99]), respectively. Our findings indicate that weight gain in pregnancy, maternal age and gestational age should be considered as independent risk factors for macrosomia.

SENP2 Regulates Hepatocellular Carcinoma Cell Growth by Modulating the Stability of β-catenin

Shen, Huo-Jian,Zhu, Hong-Yi,Yang, Chao,Ji, Fu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

SUMOylation has emerged as an important post-translational modification that modulates the localization, stability and activity of a broad spectrum of proteins. A dynamic process, it can be reversed by a family of SUMO-specific proteases (SENPs). However, the biological roles of SENPs in mammalian development and pathogenesis remain largely elusive. Here, we demonstrated that SENP2 plays a critical role in the control of hepatocellular carcinoma cell growth. SENP2 was found to be down-regulated in hepatocellular carcinoma (HCC) tissues and over-expression suppressed the growth and colony formation of HCC cells. In contrast, silencing of SENP2 by siRNAs promoted cancer cell growth. We further found that stability of ${\beta}$-catenin was markedly decreased when SENP2 was over-expressed. Interestingly, the decrease was dependent on the de-SUMOylation activity of SENP2, because over-expression of a SENP2 catalytic mutant form had no obviously effects on ${\beta}$-catenin. Our results suggest that SENP2 might play a role in hepatocellular carcinoma cell growth control by modulating the stability of ${\beta}$-catenin.

Prognostic Significance of GSTP1, XRCC1 and XRCC3 Polymorphisms in Non-small Cell Lung Cancer Patients

Ke, Hong-Gang,Li, Jun,Shen, Yi,You, Qing-Sheng,Yan, Yu,Dong, Han-Xuan,Liu, Jun-Hua,Shen, Zhen-Ya Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Aim: Individual differences in chemosensitivity and clinical outcome in non-small cell lung cancer (NSCLC) patients treatment with platinum-based chemotherapy may be due to genetic factors. Our study aimed to investigate the prognostic role of GSTP1, XRCC1 and XRCC3 in NSCLC patients treated with chemotherapy. Methods: A total of 460 cases were consecutively selected from The Affiliated Hospital of Nantong University between Jan. 2003 to Nov. 2006, and all were followed-up until Nov. 2011. Genotyping of GSTP1 Ile105Val, XRCC1 Arg194Trp, XRCC1 Arg399Gln and XRCC3 Thr241Met was conducted by duplex polymerase-chain-reaction with confronting-two-pair primer methods. Results: Patients with GSTP Val/Val exhibited a shorter survival time, and had a 1.89 fold greater risk of death than did those with the IIe/IIe genotype. For XRCC1 Arg194Trp, the variant genotype Trp/Trp was significantly associated with a decreased risk of death from NSCLC when compared with the Arg/Arg. Individuals carrying XRCC1 399Gln/Gln genotype had a longer survival time, with a lowered risk of death from NSCLC. Conclusion: This study indicated that GSTP1 Ile105Val, XRCC1 Arg194Trp and XRCC1Arg399Gln genes have a role in modifying the effect of platinum-based chemotherapy for NSCLC patients in a Chinese population. Our findings provide information for therapeutic decisions for individualized therapy in NSCLC cases.

Digital Native 척도 개발 및 검증

홍민지(Minji Hong),우선연(Sunyeon Woo),진징(Jin Jing),센이(Shen Yi),이재학(Jeahak Lee),박철(Cheol Park) 한국IT서비스학회 2010 한국IT서비스학회 학술대회 논문집 Vol.2010 No.9

본 연구는 Don Tapscott(2008)의 디지털 네이티브(Digital Native)의 8가지 특성인 자유, 맞춤제작, 철저한 조사, 성실성, 협업, 엔터테인먼트, 속도, 혁신에 대한 척도를 개발하여, 한국과 중국의 젊은 세대들을 대상으로 검증해 보았다. 설문은 한국, 중국의 10대~30대, 남녀 506명이 응답하였으며, 수집된 설문지를 통해 디지털 네이티브의 8가지 차원을 확인하였으며, 측정의 신뢰도와 타당성을 검증하였다. 또한 한국과 중국의 디지털 네이티브 성향 차이를 살펴보고 IT소비 상관관계에 대해 알아보았다. 그 결과 디지털 네이티브(Digital Native)의 8가지 요인은 IT 소비와 유의한 상관관계를 나타내었으며, 특히 엔터테인먼트, 자유, 혁신속성에서 비교적 높은 상관관계가 있는 것으로 나타났다. 또한 디지털 네이티브 성향이 높은 집단과 낮은 집단 간에는 IT 소비면에서 거의 모두 유의한 차이가 나타났다. 본 연구결과는 한국과 중국의 디지털 네이티브(Digital Native) 세대의 새로운 소비특징과 소통 방법을 연구하여 그들에게 맞는 새로운 마케팅 방향을 제시할 수 있을 것이다.

The embodiment of the strategy of “using active chemicals as excipients” in compound preparation

Yang Rizhao,Hong Yanlong,Wang Youjie,Zhao Lijie,Shen Lan,Feng Yi 한국약제학회 2022 Journal of Pharmaceutical Investigation Vol.52 No.1

Background “Using active chemicals as excipients” refers to a preparation strategy. It makes use of the drugs owning the special physical and chemical properties as the roles of excipients to assist the preparation forming or achieve some specific drug delivery function, especially in the compound preparation. Area Covered This paper proposed the concept of “using active chemicals as excipients”, expounded the advantages and potentials of “medicine as excipient’s use”, and analyzed the research status of “using active chemicals as excipients” from the aspects of chemical compound preparations and traditional Chinese medicine preparations. We classified the excipient function of drugs in formulations, and expounded the functional principles and characteristics of drugs as excipients from drug physical properties and drug dispersing forms in preparation. Expert Opinion This preparation strategy has the advantages of reducing the amount of excipients, improving drugs compliance and reducing production costs. It is an important development direction of modern pharmaceutical preparation research and production. In a word, “using active chemicals as excipients” is a potential preparation strategy, which should be paid attention to and be implemented in the process of preparation research and development.

디지털네이티브(Digital Native)척도 개발 및 검증

홍민지 ( Minji Hong ),우선연 ( Seonyeon Woo ),진징 ( Jin Jing ),센이 ( Shen Yi ),이재학 ( Jae Hak Lee ),박철 ( Cheol Park ) 고려대학교 세종경영연구소 2018 경상논집 Vol.38 No.1

본 연구는 Don Tapscott(2008)의 디지털네이티브(Digital Native)의 8가지 특성인 자유, 맞춤제작, 철저한 조사, 성실성, 협업, 엔터테인먼트, 속도, 혁신에 대한 척도를 개발하여, 한국과 중국의 젊은 세대들을 대상으로 검증해 보았다. 설문은 한국, 중국의 10대~30대, 남녀 506명이 응답하였으며, 수집된 설문지를 통해 디지털네이티브의 8가지 차원을 확인하였으며, 측정의 신뢰도와 타당성을 검증하였다. 또한 한국과 중국의 디지털네이티브 성향 차이를 살펴보고 IT소비 상관관계에 대해 알아보았다. 그 결과 디지털네이티브(Digital Native)의 8가지 요인은 IT 소비와 유의한 상관관계를 나타내었으며, 특히 엔터테인먼트, 자유, 혁신 속성에서 비교적 높은 상관관계가 있는 것으로 나타났다. 또한 디지털네이티브 성향이 높은 집단과 낮은 집단 간에는 IT 소비 면에서 거의 모두 유의한 차이가 나타났다. 본 연구결과는 한국과 중국의 디지털네이티브(Digital Native) 세대의 새로운 소비 특징과 소통 방법을 연구하여 그들에게 맞는 새로운 마케팅 방향을 제시할 수 있을 것이다. This study has developed the scale of digital native based on Don Tapscott(2008) including freedom, customization, investigation, integrity, entertainment, speed, and innovation. In addition, it was validated by collecting 506 data from Korea and China, and we confirmed the 8 dimension of digital native. There foung significant correltions between the tendency of digital native and IT consumption, especially, the factor of entertainment, freedom, and innovation showed higer correlation with IT consumption. The differences of digital native were found between Korean and Chinese. The marketing impications for digital native are suggested in conclusion.

Oxymatrine Causes Hepatotoxicity by Promoting the Phosphorylation of JNK and Induction of Endoplasmic Reticulum Stress Mediated by ROS in LO2 Cells

Gu, Li-li,Shen, Zhe-lun,Li, Yang-Lei,Bao, Yi-Qi,Lu, Hong Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.5

Oxymatrine (OMT) often used in treatment for chronic hepatitis B virus infection in clinic. However, OMT-induced liver injury has been reported. In this study, we aim to investigate the possible mechanism of OMT-induced hepatotoxicity in human normal liver cells (L02). Exposed cells to OMT, the cell viability was decreased and apoptosis rate increased, the intracellular markers of oxidative stress were changed. Simultaneously, OMT altered apoptotic related proteins levels, including Bcl-2, Bax and pro-caspase-8/-9/-3. In addition, OMT enhanced the protein levels of endoplasmic reticulum (ER) stress makers (GRP78/Bip, CHOP, and cleaved-Caspase-4) and phosphorylation of c-Jun N-terminal kinase (p-JNK), as well as the mRNA levels of GRP78/Bip, CHOP, caspase-4, and ER stress sensors (IREI, ATF6, and PERK). Pre-treatment with Z-VAD-fmk, JNK inhibitor SP600125 and N-acetyl-l-cysteine (NAC), a ROS scavenger, partly improved the survival rates and restored OMT-induced cellular damage, and reduced caspase-3 cleavage. SP600125 or NAC reduced OMT-induced p-JNK and NAC significantly lowered caspase-4. Furthermore, 4-PBA, the ER stress inhibitor, weakened inhibitory effect of OMT on cells, on the contrary, TM worsen. 4-PBA also reduced the levels of p-JNK and cleaved-caspase-3 proteins. Therefore, OMT-induced injury in L02 cells was related to ROS mediated p-JNK and ER stress induction. Antioxidant, by inhibition of p-JNK or ER stress, may be a feasible method to alleviate OMT-induced liver injury.

Influence of Curcumin on HOTAIR-Mediated Migration of Human Renal Cell Carcinoma Cells

Pei, Chang-Song,Wu, Hong-Yan,Fan, Fan-Tian,Wu, Yi,Shen, Cun-Si,Pan, Li-Qun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

Background: This study investigated the influence of curcumin on HOX transcript antisense RNA (HOTAIR)-mediated migration of cultured renal cell carcinoma (RCC) cells. Materials and Methods: Five RCC cell lines (769-P, 769-P-vector, 769-P-HOTAIR, 786-0, and Kert-3 ) were maintained in vitro. The expression of HOTAIR mRNA was determined by quantitative real-time PCR and cell migration was measured by transwell migration assay. The effects of different concentrations of curcumin (0 to $80{\mu}mol/L$) on cell proliferation was determined by the CCK-8 assay and influence of non-toxic levels (0 to $10{\mu}M$) on the migration of RCC cells was also determined. Results: Comparison of the 5 cell lines indicated a correlation between HOTAIR mRNA expression and cell migration. In particular, the migration of 769-P-HOTAIR cells was significantly higher than that of 769-P-vector cells. Curcumin at $2.5-10{\mu}M$ had no evident toxicity against RCC cells, but inhibited cell migration in a concentration-dependent manner. Conclusions: HOTAIR expression is correlated with the migration of RCC cells, and HOTAIR may be involved in the curcumin-induced inhibition of RCC metastasis.

Genome-wide Analysis of Aberrant DNA Methylation for Identification of Potential Biomarkers in Colorectal Cancer Patients

Fang, Wei-Jia,Zheng, Yi,Wu, Li-Ming,Ke, Qing-Hong,Shen, Hong,Yuan, Ying,Zheng, Shu-Sen Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Background: Colorectal cancer is one of the leading causes of mortality worldwide. Genome wide analysis studies have identified sequence mutations causing loss-of-function that are associated with disease occurrence and severity. Epigenetic modifications, such DNA methylation, have also been implicated in many cancers but have yet to be examined in the East Asian population of colorectal cancer patients. Methods: Biopsies of tumors and matched non-cancerous tissue types were obtained and genomic DNA was isolated and subjected to the bisulphite conversion method for comparative DNA methylation analysis on the Illumina Infinium HumanMethylation27 BeadChip. Results: Totals of 258 and 74 genes were found to be hyper- and hypo-methylated as compared to the individual's matched control tissue. Interestingly, three genes that exhibited hypermethylation in their promoter regions, CMTM2, ECRG4, and SH3GL3, were shown to be significantly associated with colorectal cancer in previous studies. Using heatmap cluster analysis, eight hypermethylated and 10 hypomethylated genes were identified as significantly differentially methylated genes in the tumour tissues. Conclusions: Genome-wide methylation profiling facilitates rapid and simultaneous analysis of cancerous cells which may help to identify methylation markers with high sensitivity and specificity for diagnosis and prognosis. Our results show the promise of the microarray technology in identification of potential methylation biomarkers for colorectal cancers.