FSCB phosphorylation in mouse spermatozoa capacitation

( Shun Li Liu ),( Bing Ni ),( Xiang Wei Wang ),( Wen Qian Huo ),( Jun Zhang ),( Zhi Qiang Tian ),( Ze Min Huang ),( Yi Tian ),( Jun Tang ),( Yan Hua Zheng ),( Feng Shuo Jin ),( Yan Feng Li ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.8

It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation. [BMB reports 2011; 44(8): 541-546]

Clinical Efficacy and Prognosis Factors for Advanced Hepatoblastoma in Children: A 6-year Retrospective Study

Zhang, Yi,Zhang, Wei-Ling,Huang, Dong-Sheng,Hong, Liang,Wang, Yi-Zhuo,Zhu, Xia,Hu, Hui-Min,Zhang, Pin-Wei,Yi, You,Han, Tao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Objective: This study aimed to investigate the effect of multimodality treatment of advanced paediatric hepatoblastoma (HB) and the factors affecting prognosis. Methods: A total of 35 children underwent multimodality treatments consisting of chemotherapy, surgery, interventional therapy, and autologous peripheral blood stem cell transplantation. The patients were followed up every month. Results: Serum AFP levels in 33 out of 35 patients in this study were significantly increased (P = 0.0002). According to the statistical scatter plot, the values of serum AFP on the 25th, 50th, and 75th percentages were 1,210, 1,210 and 28,318 ng/dl, respectively. Of the 35 cases, 21 were stage IV. 18 cases were treated with systemic chemotherapy before surgery, and 3 cases with locally interventional chemotherapy before surgery. Statistical analysis showed that the preferred interventional treatment affected prognosis, and that there was a statistically significant difference (P = 0.024). Some 33 patients completed the follow-up, of which 17 were in complete remission (CR), 5 were in partial remission (PR), 1 became disease progressive (DP), and 10 died. The remission and overall survival rates were 66.7% (22/33) and 69.7% (23/33), respectively. Patients with the mixed HB phenotypes had worse prognoses than the epithelial phenotype (P < 0.001), and patients in stage IV had a lower survival rate than those in stage III (P < 0.001). Conclusion: Multimodality treatment can effectively improve remission rate and prolong the survival of children with advanced HB. In addition, alpha-fetoprotein (AFP), a tumor marker of liver malignant tumors, HB pathological classification, and staging are highly useful in predicting prognosis.

CMA Adaptive Array Performance in the Presence of Correlated signals

Yi Min Zhang,Ke Hu Yang 대한전자공학회 1998 대한전자공학회 심포지엄 논문집 Vol.3 No.-

Reliability Analysis for Random MDOF Nonlinear Systems with Independence Failure Modes

Yi Min Zhang,Bang Chun Wen,Qiao Ling Lu 대한기계학회 1997 Asia-Pacific Vibration Conference Vol.2 No.1

Cross-immunizing potential of tumor MAGE-A epitopes recognized by HLA-A*02:01-restricted cytotoxic T Lymphocytes

( Ze Min Huang ),( Zheng Cai Jia ),( Jun Tang ),( Yi Zhang ),( Yi Tian ),( Dong Jing Ni ),( Fang Wang ),( Yu Zhang Wu ),( Bing Ni ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.7

Almost all melanoma cells express at least one member of the MAGE-A antigen family, making the cytotoxic T cells (CTLs) epitopes with cross-immunizing potential in this family attractive candidates for the broad spectrum of anti-melanoma immunotherapy. In this study, four highly homologous peptides (P264: FLWGPRALA, P264I9: FLWGPRALI, P264V9: FLWGPRALV, and P264H8: FLWGPRAHA) from the MAGE-A antigens were selected by homologous alignment. All four peptides showed high binding affinity and stability to HLA-A*02:01 molecules, and could prime CTL immune responses in human PBMCs and in HLA-A*02:01/Kb transgenic mice. CTLs elicited by the four epitope peptides could cross-lyse tumor cells expressing the mutual target antigens, except MAGE-A11 which was not tested. However, CTLs induced by P264V9 and P264I9 showed the strongest target cell lysis capabilities, suggesting both peptides may represent the common CTL epitopes shared by the eight MAGE-A antigens, which could induce more potent and broad-spectrum antitumor responses in immunotherapy. [BMB Reports 2012; 45(7): 408-413]

HIIT 프로그램 적용이 비만 여중생의 신체구성과 체력에 미치는 효과

장징이(Zhang, Jing Yi),김창영(Kim, Chang Young),이민기(Lee, Min Ki),안민지(An, Min Ji) 학습자중심교과교육학회 2018 학습자중심교과교육연구 Vol.18 No.11

이 연구는 8주 동안의 HIIT(고강도 인터벌 트레이닝) 프로그램의 적용이 비만 여자 중학생의 신체구성과 체력에 미치는 효과를 분석하여, 학교 현장에서 청소년 비만을 예방하고 관리하는데 필요한 기초 자료를 제공하고자 한다. 연구 목적을 달성하기 위하여 충북 C시에 소재한 K중학교 2학년 여학생 24명을 임의로 선정한 후, 운동집단과 통제집단에 각각 12명씩 무선 배정하고 고강도 인터벌 트레이닝 전ㆍ후 신체구성과 체력을 측정하였다. 고강도 인터벌 트레이닝은 주3회 20분씩, 8주간 실시하였으며, t검증을 이용해 집단 내, 집단 간 차이를 분석하였으며, 연구 결과는 다음과 같다. 첫째, 실험 후 운동집단의 체중과 체지방률은 유의하게 감소하였고, 체지방 체중에는 차이가 나타나지 않았다. 둘째, 실험 후 운동집단의 체지방률이 통제집단에 비해 통계적으로 유의하게 감소하였다. 셋째, 실험 후 운동집단의 심폐지구력과 근지구력이 유의한 차이로 향상되었으며, 근력, 유연성, 순발력에서는 차이가 나타나지 않았다. 넷째, 운동집단의 심폐지구력이 통제집단에 비해 통계적으로 유의하게 향상되었다. The aim of this study was to investigate the influence of the exercise program that applied the 8 weeks of HIIT method on the body composition and physical fitness in obese middle school students. In order to accomplish the aim of this study, 24 2nd grade female students at K middle school located in Cheongju city, Chungbuk-do, were selected at random and the allocated 12 students each for the exercise group and control group and measured body composition and physical fitness before and after the HIIT. HIIT program is implemented for twenty minutes of three times each week for 8 weeks and the t-test was used to analyze the difference in the group and between the groups. Looking into the changes of body composition before and after the exercise group that participated regularly to the 8-week HIIT program, the weight and % body fat were noticeably reduced after the experiment and there was no difference in the fat free mass. Looking into the difference of exercise group and control group, the % bofy fat of the exercise group after the experiment was noticeably reduced statistically compared to the control group. Looking into the changes of physical fitness before and after the exercise group that participated regularly to the 8-week HIIT program, the cardiorespiratory endurance and muscular endurance of the exercise group after the experiment was noticeably improved and there was no difference with respect to the muscle strength, flexibility, and agility. Looking into the difference of exercise group and control group, the cardiorespiratory endurance of the exercise group after the experiment was noticeably improved statistically compared to the control group.

Covalent Immobilization of Penicillin G Acylase onto Fe₃O₄@Chitosan Magnetic Nanoparticles

( Xiao Min Ling ),( Xiang Yu Wang ),( Ping Ma ),( Yi Yang ),( Jie Mei Qin ),( Xue Jun Zhang ),( Ye Wang Zhang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.5

Penicillin G acylase (PGA) was immobilized on magnetic Fe₃O₄@chitosan nanoparticles through the Schiff base reaction. The immobilization conditions were optimized as follows: enzyme/support 8.8 mg/g, pH 6.0, time 40 min, and temperature 25℃. Under these conditions, a high immobilization efficiency of 75% and a protein loading of 6.2 mg/g-support were obtained. Broader working pH and higher thermostability were achieved by the immobilization. In addition, the immobilized PGA retained 75% initial activity after ten cycles. Kinetic parameters Vmax and Km of the free and immobilized PGAs were determined as 0.113 mmol/min/mg-protein and 0.059 mmol/min/mg-protein, and 0.68 mM and 1.19 mM, respectively. Synthesis of amoxicillin with the immobilized PGA was carried out in 40% ethylene glycol at 25℃ and a conversion of 72% was obtained. These results showed that the immobilization of PGA onto magnetic chitosan nanoparticles is an efficient and simple way for preparation of stable PGA.

A Wide-gain-range Intermediate Frequency Integrated Circuit for a Superheterodyne Receiver

Changchun Zhang,Jingjian Zhang,Ying Zhang,Yi Zhang,Jie Liu,Sung Min Park 대한전자공학회 2018 Journal of semiconductor technology and science Vol.18 No.4

A wide-gain-range Intermediate Frequency (IF) integrated circuit is designed and implemented for a superheterodyne receiver in standard 0.18 μm CMOS technology. It consists mainly of a programmable attenuator, a Variable Gain Amplifier (VGA), a downconverting mixer and a fixed gain amplifier. The π-type-network attenuator is chosen over a T-type network one in order for a compact layout. The common-gate-input VGA is used for a high operation frequency, a small phase shift and a wide gain range. The common-gate Gilbert-type mixer with a dynamic current injection technique and a capacitive cross-coupling technique is employed for proper gain, low noise and high linearity. Furthermore, a Phase-Locked Loop (PLL) is also integrated monolithically so as to generate the desired local clock signal for the mixer. Measurement results show that, from an area of 2.16 mm×1.46 mm and power consumption of 234 mW, the IF chip is able to operate properly with a gain range of 5 dB ~ 57 dB, output power of above 0dBm, and so on. In addition, the on-chip local clock with an operation frequency of 660 MHz and a phase noise of -119.34 dBc @1 MHz is achieved.

Identification and evolutionary history of the DD41D transposons in insects

Xiao-Gu Zhang,Hua-Hao Zhang,Yi-Hong Shen,Xiao-Min Xiong,Min-Jin Han 한국유전학회 2016 Genes & Genomics Vol.38 No.2

The rosa monophyletic group of transposons is a group of transposable element with characteristics of encoding a DD41D motif in the catalytic domain. However, biology and evolutionary history of this monophyletic group are still poorly understood. In this study, we report the first description for the presence of a rosa transposon in the silkworm Bombyx mori. Further analyses confirmed that this element in the silkworm genome had recently amplified and might still be capable of transposition. In addition, we present evidence, based on searches of publicly available insect genomes, that a new clade of the rosa monophyletic group was identified. Interestingly, analysis of their three dimensional structures suggested that these proteins showed highly similar protein structures with that of the Mos1 transposase. These results provided useful insights into the functionality of these transposases and their structural and functional deviations from other transposases in the Tc1/mariner superfamily. Meanwhile, sequence and phylogenetic analysis confirmed that DD41D and maT elements might represent another independent large group of the Tc1/mariner superfamily. Importantly, the result of the comparison of terminal inverted repeats (TIRs) validated that DD41D and maT elements almost had identical consensus terminal sequences (50-CAGGGTGNS NCA-30), implying they might have similar cleavage sites or patterns during the process of their transposition. In a word, this study will enrich and expand our knowledge of the Tc1/mariner superfamily.

A Meta-Analysis of the Accuracy of Prostate Cancer Studies Which Use Magnetic Resonance Spectroscopy as a Diagnostic Tool

Peng Wang,You-min Guo,Min Liu,Yong-qian Qiang,Xiao-juan Guo,Yi-li Zhang,Xiao-Yi Duan,Qiu-Juan Zhang,Weifeng Liang 대한영상의학회 2008 Korean Journal of Radiology Vol.9 No.5

Objective: We aimed to do a meta-analysis of the existing literature to assess the accuracy of prostate cancer studies which use magnetic resonance spectroscopy (MRS) as a diagnostic tool. Materials and Methods: Prospectively, independent, blind studies were selected from the Cochrane library, Pubmed, and other network databases. The criteria for inclusion and exclusion in this study referenced the criteria of diagnostic research published by the Cochrane center. The statistical analysis was adopted by using Meta-Test version 6.0. Using the homogeneity test, a statistical effect model was chosen to calculate different pooled weighted values of sensitivity, specificity, and the corresponding 95% confidence intervals (95% CI). The summary receiver operating characteristic (SROC) curves method was used to assess the results. Results: We chose two cut-off values (0.75 and 0.86) as the diagnostic criteria for discriminating between benign and malignant. In the first diagnostic criterion, the pooled weighted sensitivity, specificity, and corresponding 95% CI (expressed as area under curve [AUC]) were 0.82 (0.73, 0.89), 0.68 (0.58, 0.76), and 83.4% (74.97, 91.83). In the second criterion, the pooled weighted sensitivity, specificity, and corresponding 95% CI were 0.64 (0.55, 0.72), 0.86 (0.79, 0.91) and 82.7% (68.73, 96.68). Conclusion: As a new method in the diagnostic of prostate cancer, MRS has a better applied value compared to other common modalities. Ultimately, large scale RCT randomized controlled trial studies are necessary to assess its clinical value. Objective: We aimed to do a meta-analysis of the existing literature to assess the accuracy of prostate cancer studies which use magnetic resonance spectroscopy (MRS) as a diagnostic tool. Materials and Methods: Prospectively, independent, blind studies were selected from the Cochrane library, Pubmed, and other network databases. The criteria for inclusion and exclusion in this study referenced the criteria of diagnostic research published by the Cochrane center. The statistical analysis was adopted by using Meta-Test version 6.0. Using the homogeneity test, a statistical effect model was chosen to calculate different pooled weighted values of sensitivity, specificity, and the corresponding 95% confidence intervals (95% CI). The summary receiver operating characteristic (SROC) curves method was used to assess the results. Results: We chose two cut-off values (0.75 and 0.86) as the diagnostic criteria for discriminating between benign and malignant. In the first diagnostic criterion, the pooled weighted sensitivity, specificity, and corresponding 95% CI (expressed as area under curve [AUC]) were 0.82 (0.73, 0.89), 0.68 (0.58, 0.76), and 83.4% (74.97, 91.83). In the second criterion, the pooled weighted sensitivity, specificity, and corresponding 95% CI were 0.64 (0.55, 0.72), 0.86 (0.79, 0.91) and 82.7% (68.73, 96.68). Conclusion: As a new method in the diagnostic of prostate cancer, MRS has a better applied value compared to other common modalities. Ultimately, large scale RCT randomized controlled trial studies are necessary to assess its clinical value.