Overexpression of a Phosphatidylinositol-Specific Phospholipase C Gene from Populus simonii ×  P. nigra Improves Salt Tolerance in Transgenic Tobacco

Sun Yao,Li Yao,Sun Xin,Wu Qiong,Yang Chunhui,Wang Lei 한국식물학회 2022 Journal of Plant Biology Vol.65 No.5

Phosphatidylinositol-specific phospholipase C (PI-PLC) catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate into diacylglycerol and inositol 1,4,5-trisphosphate. It can play an essential role in plant stress response and signaling. However, the functions of PLCs remain unclear in wooden plants. This study carried out the bioinformatics analysis of the PLC gene family in poplar. The expression pattern analysis suggested the transient up-regulation of PsnPLC in salt stress conditions. The transgenic tobacco plants overexpressing PsnPLC were generated by the Agrobacterium-mediated leaf disc method. The transgenic lines showed a significant increase in plant height, SOD and POD activity, and proline content under salt stress. By contrast, transgenic lines showed a decreased malondialdehyde content under salt stress. Comparative transcriptome analysis indicated that the overexpression of PsnPLC mainly affects membrane-associated GO terms and signaling pathways, and the differential expression of salt-responsive genes may contribute to the enhanced salt tolerance. Overall, the results indicated that PsnPLC plays an essential role in modulating salt tolerance in transgenic tobacco.

AutoScale: Adaptive QoS-Aware Container-based Cloud Applications Scheduling Framework

( Yao Sun ),( Lun Meng ),( Yunkui Song ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.6

Container technologies are widely used in infrastructures to deploy and manage applications in cloud computing environment. As containers are light-weight software, the cluster of cloud applications can easily scale up or down to provide Internet-based services. Container-based applications can well deal with fluctuate workloads by dynamically adjusting physical resources. Current works of scheduling applications often construct applications’ performance models with collected historical training data, but these works with static models cannot self-adjust physical resources to meet the dynamic requirements of cloud computing. Thus, we propose a self-adaptive automatic container scheduling framework AutoScale for cloud applications, which uses a feedback-based approach to adjust physical resources by extending, contracting and migrating containers. First, a queue-based performance model for cloud applications is proposed to correlate performance and workloads. Second, a fuzzy Kalman filter is used to adjust the performance model’s parameters to accurately predict applications’ response time. Third, extension, contraction and migration strategies based on predicted response time are designed to schedule containers at runtime. Furthermore, we have implemented a framework AutoScale with container scheduling strategies. By comparing with current approaches in an experiment environment deployed with typical applications, we observe that AutoScale has advantages in predicting response time, and scheduling containers to guarantee that response time keeps stable in fluctuant workloads.

Fault-tolerant Model Predictive Current Control of Six-Phase Permanent Magnet Synchronous Motors with Pulse Width Modulation

Yao Ming,Peng Jingyao,Sun Xiaodong,Sun Yueping 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.3

A model predictive current control strategy with pulse width modulation is proposed for a single-phase open circuit fault. This method decomposes the change matrix based on the normal vector space to ensure that the decoupling matrix remains unchanged so that there is no need to reconfigure the controller topology. By analysing the fault phase voltage difference under normal and fault conditions, 24 synthetic virtual voltage vectors are used to compensate the voltage vector during a single-phase fault and eliminate the current coupling problem. In addition, a standard pulse width modulation switching sequence is implemented to achieve the purpose of unifying the switching frequency, which makes the method easy to implement. Finally, the effectiveness of the proposed method is verified by experiments.

Capacitor commutation type DC circuit breaker with fault character discrimination capability

Yao Sun,Yanfang Fan,Junjie Hou 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.6

Flexible direct current (DC) grids face a serious challenge in terms of rapidly isolating DC faults. A DC circuit breaker is an effective solution for DC fault isolation. To improve the fault-isolation and reclosing capability of fl exible DC systems, a new high voltage direct current (HVDC) circuit breaker topology with adaptive reclosing capability is proposed in this paper. The topology of the circuit breaker is a T-shaped structure, which has the ability to break the current in both directions and effectively reduce the cost of components. Meanwhile, after the fault is cleared, the circuit breaker is controlled to inject a voltage signal into the line. Based on this, to prevent the circuit breaker reclosing in the event of a permanent fault from having an impact on the system, a method for fault identification based on Euclidean distance that uses the voltage signal to identify the fault properties is proposed. Finally, the performance of the circuit breaker is simulated and verified by PSCAD/EMTDC simulation software, and compared with the typical existing circuit breakers to verify the effectiveness of the circuit breaker and reclosing scheme.

Influence of Copper Base Foliar Fertilizer and Controlled Release Foliar Fertilizer on Yields, FruitQuality, Mineral Nutrition and Leaf Antioxidant Enzyme Activity of Pepper (capsicum Annuum L.)

Yao Sun,Min Zhang 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

An Empirical Study on Self-service Technologies and Consumer Satisfaction

Sun Yao 동아시아무역학회 2020 Journal of East Asian Trade(JEAT) Vol.2 No.2

Purpose - This research examines what factors principally affect consumers’ acceptance of Self-Service Technologies and intent to use. Design/Methodology/Approach - To answer the research question, the TAM was adopted. We collected data from market research. By adopting TAM, we examine the factors that affect the continuous use intention of Self-Service Technologies. Findings - The empirical results show that SSTs’ usefulness and ease of use positively affect consumers’ intension to use in line with the proposed assumption. Research Implications - The perceived usefulness and perceived ease of use positively affect continuous use intention. The Self-Service provider would enhance usefulness and ease of use, then increase the likelihood of adopting Self-Service Technologies.

Study of One Kind of Extended Proportional Guidance Law

Yao Jun,Feng Hai-tao,Sun Peng 보안공학연구지원센터 2014 International Journal of Hybrid Information Techno Vol.7 No.6

One kind of extended proportional guidance law is presented. This guidance law can be used without restriction about the motion speed of missile and target, and this is a different point with the classical proportional guidance law. In the case of random alter the tracking target curvature radius, compared with classical proportional guidance law under the proper random quantity, the normal overload of trajectory is relatively small, and has certain advantage, so the study has significant reference value in actual application. And interception time and normal overload have good performance. Tracking performance for high maneuverability target is better than the classical proportional guidance law. A target intercepting simulation based on MATLAB of the proportional coefficients and line-of-sight angular velocities is generated to analyze the trajectory properties. The effect of guidance coefficients on trajectory properties is studied. Through simulation, we simplify the problem by ignoring the actual factors so that a certain level of error is expected. The situation in practical requires more in-depth analysis and research.

ANXA2 Regulates the Behavior of SGC-7901 Cells

Sun, Meng-Yao,Xing, Rui-Huan,Gao, Xiao-Jie,Yu, Xiang,He, Hui-Min,Gao, Ning,Shi, Hong-Yan,Hu, Yan-Yan,Wang, Qi-Xuan,Xu, Jin-Hui,Hou, Ying-Chun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

ANXA2, a member of the annexin family, is overexpressed and plays important roles in tumor development. However, the significance of ANXA2 expression in gastric carcinoma has not been clarified.To elucidate its roles in growth of gastric cancer, ANXA2 expression in SGC-7901 cells was inhibited with a designated siRNA, then cell proliferation, cell cycling, apoptosis and motility were determined by MTT assay, flow cytometry, Hoechst 33342 staining and wound healing assay, respectively. To further assess the behavior of ANXA2 deleted SGC-7901 cells, changes of microstructures were observed under fluorescence microscopy, laser scanning confocal microscopy and electron microscopy. We found that inhibition of ANXA2 expression caused cell proliferation to decrease significantly with G1 arrest, motility to be reduced with changes in pseudopodia/filopodia structure and F-actin and ${\beta}$-tubulin expression, and apoptosis to be enhanced albeit without significance. At the same time, ANXA2 deletion resulted in fewer pseudopodia/filopodia, non-stained areas were increased, contact inhibition among cells reappeared, and expression of F-actin and ${\beta}$-tubulin was decreased, with induction of polymerized disassembled forms. Taken together, these data suggest that ANXA2 overexpression is important to maintain the malignancy of cancer cells, and this member of the annexin family has potential to be considered as a target for the gene therapy of gastric carcinoma.

Robust Output Feedback Consensus of High-order Multi-agent Systems with Nonlinear Uncertainties

Yao Yu,Na Li,Liang Sun,Jian Liu,Changyin Sun 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.2

In this paper, the output feedback consensus problem of high-order multi-agent systems with nonlinear uncertainties is researched by the robust control method. By the dimensional extension of the observation matrix, the consensus control problem is transformed into a stability problem. Then the robust controller is designed by combining the nominal controller and the robust compensator. The nominal controller can obtain desired nominal performance based on the output informations. The robust compensator, which relys on robust signal compensation technology, is order to suppress the nonlinear uncertainties. According to the proposed method, output consensus error can be guaranteed as small as desired. Finally, simulation results are given to illustrate the effectiveness of this control method.

Electrical Measurements of an AlGaN/GaN High-electron-mobility Transistor Structure Grown on Si

Zhi-Yao Zhang,Shun-Tsung Lo,Li-Hung Lin,Kuang Yao Chen,J. Z. Huang,Zhi-Hao Sun,C.-T. Liang,N. C. Chen,Chin-An Chang,P. H. Chang 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.9

We report on magnetotransport results for an Al_0.15Ga_0.85N/GaN high-electron-mobility-transistor structure grown on a p-type Si (111) substrate. Our results show that there exists an approximately temperature (<i>T</i>)-independent point, which could be ascribed to a direct transition from a weak insulator to a high Landau level filling factor quantum Hall state, exists in the longitudinal resistivity ρ_xx. The Hall resistivity decreases with increasing <i>T</i>, compelling experimental evidence for electron-electron interaction effects in a weakly-disordered two-dimensional (2D) system. We find that electron-electron interaction effects can be estimated and eliminated, giving rise to a corrected nominally temperature-independent Hall slope. By fitting the low-field magnetotransport data to conventional 2D weak localization theory, we find that the dephasing rate 1/τ_φ is proportional to <i>T</i>. Moreover, 1/τ_φ is finite as <i>T</i> → 0, evidence for zero-temperature dephasing in our system.