Some Multi-sublinear Operators on generalized Morrey spaces with non-doubling measures

Yanlong Shi,Xiangxing Tao 대한수학회 2012 대한수학회지 Vol.49 No.5

In this paper the boundedness for a large class of multi-sublinear operators is established on product generalized Morrey spaces with non-doubling measures. As special cases, the corresponding results for multilinear Calderon-Zygmund operators, multilinear fractional inte-grals and multi-sublinear maximal operators will be obtained.

SOME MULTI-SUBLINEAR OPERATORS ON GENERALIZED MORREY SPACES WITH NON-DOUBLING MEASURES

Shi, Yanlong,Tao, Xiangxing Korean Mathematical Society 2012 대한수학회지 Vol.49 No.5

In this paper the boundedness for a large class of multi-sublinear operators is established on product generalized Morrey spaces with non-doubling measures. As special cases, the corresponding results for multilinear Calder$\acute{o}$n-Zygmund operators, multilinear fractional integrals and multi-sublinear maximal operators will be obtained.

Surface-Displayed Porcine IFN-λ3 in Lactobacillus plantarum Inhibits Porcine Enteric Coronavirus Infection of Porcine Intestinal Epithelial Cells

Yong-Shi Liu,Qiong Liu,Yanlong Jiang,Wentao Yang,Hai-Bin Huang,Chun-Wei Shi,Gui-Lian Yang,Chun-Feng Wang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN- λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

Mycobacterium vaccae induces a strong Th1 response that subsequently declines in C57BL/6 mice

Lijiao Zhang,Yanlong Jiang,Ziyin Cui,Wentao Yang,Limin Yue,Yingcong Ma,Shaohua Shi,Chun-fang Wang,Chun-feng Wang,Aidong Qian 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4

Mycobacterium (M.) vaccae is a fast-growing species of saprophytic bacteria that is widely distributed. To understand the host immuneresponses induced by M. vaccae isolated from bovine submaxillary lymph nodes, C57BL/6 mice were infected with reference strain M. bovisBacillus Calmette-Guérin (BCG) and isolated M. vaccae using intraperitoneal injections. Comparison of the bacterial replication and organpathology between M. vaccae and M. bovis BCG revealed that M. vaccae was more malignant than M. bovis in mice. We also demonstratedthat serum from the M. vaccae-infected mice contained a higher expression level of gamma-interferon (IFN-), tumor necrosis factor alpha,monocyte chemoattractant protein-1, interleukin (IL)-4, IL-12, IL-10 and transforming growth factor beta than did the other groups, especiallyafter week 4. Furthermore, when the numbers of CD3+CD4+IFN-+ and CD3+CD4+IL4+ cells in the infected mice were observed by flowcytometry, we found that a powerful T helper 1 (Th1) response was induced by M. vaccae infection, which was associated with the emergenceof CD3+CD4+IFN-+ cells. However, the Th1 response declined over time, which was associated with appearance of the CD4+CD25+FoxP3+and CD4+CD25+CD152+Treg cell reaction. In addition, a strong Th2 response was found. Finally, we found that M. vaccae infection increasedthe production of type I IFNs, which was associated with a reduced Th1 response.

Modulation electronic structure of NiS nanoarray induced by Fe, V doping for high efficiency water and urea electrolysis

Xiaojuan Feng,Yanlong Shi,yuanyuan Chen,Zhirong Xu,Haiyu Guan 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

Exploring high-efficient and stable low-cost electrocatalysts is of significant importance for boosting theefficiency of water splitting and purifying urea-enriched wastewater. Herein, bimetallic doping strategywas adopted to obtain jasminum nudiflorum-like Fe, V doped NiS arrays (Fe, V-NiS/NF) via typicalhydrothermal process and subsequent anion exchange reaction. The as-obtained Fe, V-NiS/NF array displayshigh catalytic activity and stability toward oxygen evolution reaction (OER) and urea oxidationreaction (UOR) in alkaline media, with reduced overpotentials of 273 and 214 mV to deliver the currentdensity of 50 mA cm2 for OER and UOR, respectively. More notably, when employing Fe, V-NiS/NF assymmetric electrolytic cell for urea electrolysis, a low cell voltage of 1.45 V is needed at 10 mA cm2,which is about 110 mV lower than the conventional water electrolysis. Meanwhile, the catalyst also displayssuperior stability for over 72 h. Such outstanding performance is attributed to the following points:(i) 3D porous flower-like structure facilitates the mass transfer and abundant exposure of active sites; (ii)in situ growth of catalysts on conductive substrate and the effective interface engineering of differentcomposition shorten the charge transport pathways and expedite electron transfer. Density functionaltheory calculations demonstrate that the Fe and V dopants regulate the electronic environment of Ni sitesand optimize the adsorption free energy of urea. This work provides a universal pathway to design highefficientand non-noble electrocatalysts for H2 production in an energy-saving way via urea electrolysis.

Designing cactus-like Fe-P doped CoNi-S arrays as highly efficient electrocatalyst for overall water splitting

Xiaojuan Feng,Yanlong Shi,Jingmei Liang,Wei Li,Xuyuan Gou 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.118 No.-

Exploring high activity, stable and low-cost electrocatalysts are of great importance for high-efficienthydrogen production via water splitting. Herein, a cactus-like Fe, P co-doped CoNi-S arraywas constructedvia facile hydrothermal reaction and subsequently sulfuration and phosphorization process. Benefitingfrom the strong synergistic effects of different components, high conductivity of bimetallic sulfide, 3Dcactus-like architecture with rich channels for mass/charge transport, and Fe and P co-doping with electronstructure regulation, the as-prepared Fe, P-NiCo2S4/NF presents superior electrocatalytic activity withlow overpotentials of 139 and 290 mV to drive the current density of 10 and 40 mA cm2 for HER and OER,respectively. Meanwhile, it also manifests the distinguished durability for over 60 h in alkaline media. Notably, when integrating in a two-electrode electrolytic cell with Fe, P-CoNi-S, a reduced operation voltageof 1.55 V is required to reach 10 mA cm2, which is superior to precious metal-based Pt/C/NF//RuO2/NFcounterparts. Impressively, it can also be driven by sustainable solar and thermal energies motivatedStirling engine. This work offers a universal strategy for designing high-activity and durable electrocatalystsby dual doping heteroatoms and synergistic interface engineering.

Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice

Yu-Ying Liu,Wentao Yang,Shaohua Shi,Ya-Jie Li,Liang Zhao,Chunwei Shi,Fangyu Zhou,Yanlong Jiang,Jingtao Hu,Wei Gu,Gui-Lian Yang,Chun-feng Wang 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2

Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 109 colony-forming unit/200 mL dose of the recombinant L. plantarum strain, and the ratios and numbers of CD11c+, CD3+CD4+, CD3+CD8+, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.

Immunomodulatory Properties of Lactobacillus plantarum NC8 Expressing an Anti-CD11c Single-Chain Fv Fragment

( Jing Liu ),( Guilian Yang ),( Xing Gao ),( Zan Zhang ),( Yang Liu ),( Xin Yang ),( Chunwei Shi ),( Qiong Liu ),( Yanlong Jiang ),( Chunfeng Wang ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.1

The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer’s patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of IL-4+ and IL-17A+ T helper (Th) cells in MLNs, as well as production of B220+ IgA+ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.