한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

고감도 CaSO_4 : Dy TL 분말의 TL 특성 및 고감도 TL 소자 제작 Dy TL powder and development of high sensitivity CaSO_4 Dy TL pellet

양정선,박재우,김두영,김장렬,장시영 제주대학교 방사능이용연구소 2002 연구보고 Vol.16 No.-

CaSO_4 : Dy series TL material is widely used as a personal dosimeter because of its high sensitivity to radiation. KAERI(Korea Atomic Energy Research Institute) has been produced CaSO_4 TL powder activated by Dy(rare earth elements) for several years and continually tried to improve its sensitivity having more than that of commercialized Teledyne CaSO_4 : Dy TL powders(USA). The TL powder is prepared by adding 0.1mol% Dy as an activator and by the acid evaporation method at first. And new step of washing this powder by distil-water is appended to separate non-crystalized powder in the initial crystal of CaSO_4 : Dy powder. CaSO_4 : Dy crystal TL powder which is produced by applying this new method has higer sensitivity than before washing step, and it has also higher sensitivity than that of Teledyne CaSO_4 : Dy TL powder. This CaSO_4 : Dy TL powder shows good TL characteristics such as dose response, energy response and fading. It is not possible to make solid detectors of only CaSO_4 : Dy TL phosphor without any binding material. Until now widely used sintered pellets. which were made from a mixture of CaSO_4 : Dy TL phosphor and Teflon. But, due to reduction in content of CaSO_4 : Dy TL phosphor in pellets(15∼20wt%) the TL sensitivity of pellets was reduced correspondingly. To variously use the CaSO_4 : Dy TL phosphor, research for preparing detectors by mixing CaSO_4 : Dy TL phosphor with different binding substances has been conductedThis paper described development of a new type of CaSO_4 : Dy pellets by using P-compounds as a bonding material(KCT-300), and compared the TL sensitivity with that of the commercialized Teledyne CaSO_4 : Dy pellets. Sensitivity of a new developed KCT-300 shows about 6 times than Teledyne ones, and can be used to measure very low radiation dose.

수도권 지역의 의료기기 관련 업체의 실태 조사 연구

양태홍,이우철,김정래,박승환 서울보건대학 1997 서울보건대학 부설 병원경영연구소 논문집 Vol.3 No.1

Recently, the domestic business of medical equipment have many difficult things in the development and sale of new product. To overcome them, it is needed to solidify the co-work system of industry-education marriage. This paper is undertakened to serve an aid in the field of medical equipment. The analysis of data is attempted through the survey by questionaires, and an object of study is the medical business corporation at Seoul area. As a result to review the data it was identifided that this study is able to be applied to the basis material to reconstruct a right role and phase to medical business Also, it`s expected that we're able to use its results as the educational materials to bring up the more qualified students fit to the industrial need.

용융 알미늄합금의 산화피막 강도측정에 관한 연구

우정명,양중식,백영남 慶熙大學校 材料科學技術硏究所 1991 材料科學技術硏究論集 Vol.4 No.-

This study carrys out to determine the strength of oxide skins of Al alloy melts as the practical alloy for automobile piston. This has been observed by Rotational Die Movement Equiment for the temperature range from 600℃ to 750℃ and oxidation time range from 10min to 60min. This method was able to quantify the strength of oxide skins of the molten alloys and had ±3% experimence error range. This strength of oxide skins of the molten alloys were much more affected by oxidation temperature than oxidation time.

박실지와 연당지의 植物相과 그들의 保護

鄭宇珪,孔榮植,金哲壽,梁運眞 경남대학교 환경문제연구소 1996 환경연구 Vol.18 No.-

1990년 7월부터 1996년 8월까지 16회에 걸쳐 慶南 陝川郡 龍州面 박실지와 연당지 및 그 周邊 地域의 水生植物과 植物相을 조사한 결과는 다음과 같았다. 1. 조사된 總 植物은 38目 107科 287屬 365種 1亞種 61變種 3品種 總 430種類로 구성되었다. 2. 이들 중 水生植物이 11目 22科 41種 10變種 1品目 總 52 種類 12.1%이고, 挻水植物 25種類 5.8%, 浮葉植物 15種類 3.5%, 沈水植物 9種類 2.5%, 浮生植物 3種類 0.8%였다. 3. 陸上植物은 36目 92科 269屬 324種 1亞種 51變種 2品種 總 378種類 87.0%였고, 이들 중에는 濕生植物이 50種類 13.2%, 水邊植物이 328種類 86.8%였다. 4. 박실지의 溜池에서 優占種은 마름, 準優占種은 줄이었고, 연당지에서의 優占種은 줄이었고 準優占種은 연이었다. 5. 희귀식물인 가시연꽃과 통발, 북방분자인 돌단풍과 도루박이 및 최근에 국내에 분포하는 것이 확인된 민초피나무의 분포가 논의되었다. The flora and distribution of hydrophytes and terrestrial plants from Paksil and Yundang swamp and their adjacent area in Hapchon-hun. Kyongsangnam-do were investigated at sixteen times from July. 1990 to August. 1996. The flora was composed of 38 orders. 107 families. 287 genera. 365 species. 1 subspecies, 61 varieties, and 3 forma, total 430 taxa. Among them, the vascular hydrophytes were composed of 11 orders. 22 families, 31 genera, 41 species, 10 varieties, and 1 forma, total 52 taxa. 12.1%. These were composed of 25 taxa emerged plants. 15 taxa floating-leaves plants. 9 taxa submerged plants, and 3 taxa free-floating plants. The terrestrial plants were composed of 36 orders. 92 families. 269 genera. 324 species. 1 subspecies. 51 varieties, and 2 forma. total 378 taxa. 87.9%. Among them, the hygrophytes and water side plants were 50 taxa, 13.2% and 328 taxa, 86.8%. In Paksil swamp dominent species was Trapa japonica and subdominent was Zizania latiforia on the whole. In Yundang swamp dominent species was Zizania latiforia and subdominent species was Nelumbo nucifera on the whole. The distribution of Euryale ferox. Scirpus radicans. Acerphyllum rossii. Utricularia Japonica. Xanthoxylum piperitum var. inerme. were discussed. Also the work protection of Paksil swamp were discussed.

NiTi-형상기억합금 차동식 액츄에이터의 동특성연구

정상화,김현욱,장우양,김경석,차경래,나윤철 朝鮮大學校 機械技術硏究所 2001 機械技術硏究 Vol.4 No.2

최근 전 세계적으로 초소형 정밀 기계 기술에 관한 연구 및 개발이 활발하게 이루어지면서 형상기억합금(Shape Memory Alloy : SMA)을 이용한 초소형 액츄에이터(Micro Actuator)에 관한 관심이 증대되고 있다. 형상기억합금을 액츄에이터에 응용하기 위해서는 동적 특성의 분석이 필수적이지만 지금까지의 연구에서는 형상기억합금의 물질 및 재료적 특성은 많이 보고되고 있으나 동적 특성에 대한 연구는 매우 부족한 실정이다. 본 논문에서는 비저항(resistivity)이 큰 NiTi합금 와이어(wire)를 이용하여 나선형 스프링(helical spring)을 제작하여 디지털 힘 측정기(Digital force gauge)와 적외선 온도센서(Infrared themometer) 그리고 레이저 변위 센서(Laser displacement sensor)를 이용하여 이 NiTi 스프링의 힘의 크기와 반응속도, 온도와 변위를 측정하여 형상기억합금의 동적 특성을 분석하였다. 또한 형상기억합금을 이용하여 차동식 액츄에이터를 제작하여 그 동적 성능을 실험하였다. In the recent years, as the research and the development of micro and precision machinery become active, the interest of micro actuators using SMA(Shape Memory Alloy) has been increased. The dynamic characteristic analysis of SMA is necessary for actuator application and many common researches report the material characteristics of SMA sufficiently. However, the research on dynamic characteristics is very deficient. In this paper, the helical spring are fabricated with NiTi SMA wire of high resistivity. The force, response speed, temperature, and displacement are measured by digital force gauge, infrared thermometer, and laser displacement sensor so that the dynamic characteristics of this SMA is analyzed. Also, bidirectional actuator was fabricated and experimented for its performance.

대장암종에서 암관련 유전자들의 Promoter Methylation과 암종 발생 및 진행과의 관게에 대한 연구

정동준,이정은,민영기,조성우,백무준,양승하,김의한,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal corcinoma is one of the most common malignant tumor in Korea and its carcinogenic mechanism has not been elucidated exactly yet. So far, the most common carcinogenic mechanism has been known to be multiple genetic changes. But, the CpG island hypermethylation has been revealed to be one of the most important carcinogenic mechanism second to the genetic changes. The epigenetic changes characteristically imprint and transfer to the next generation without changes of nucleotide sequences. When the CpG island of promoter or 5'- exon 1 of a specific gene is methylated by DNA methyltrasferases (DNMTs), the gene expression is inhibited. Carcinogenic mechansisms by promoter methylation has been well known, but the progression of the cancer has not been elucidated yet. The molecular researches on the carcinogenic mechanisms largely depended on the tumor tissue containing various components of cell, due to difficulties in harvesting pure homogeneous tumor cells. Promoter methylatonof the cancer related genes as APc, hMLH2, p16, DAP-K and MGMT in 50 cases of sporadic colorectal carcinoma was studied. The pure normal colorectal epithelial cells, and cancer cells from superficial and deep invasive portion were harvested respectively by laser captured microdissection(LCM), and DNA was extracted from them. The promoter methylation was studied by methylation specific poymerase reaction (MSP) after bisulfite modification of the DNA and the expression of the genes by immunohistochemistry. The results suggests that promoter methylation of the cancer related genes are closely related with the colorectal carcinogenesis and it occurrs from early phase of carcinogenesis, progressing constantly to the late stage. And the promoter methylation of normal epithelial cells increased by age. The p15, known to be the genes related to hematologic malignancy, was closely related to the colorectal carcinoma as well.

Li 화합물을 첨가한 CaSO₄:Dy 열중성자 측정용 TLD 소자 개발

양정선,김두영,박재우,김장렬,장시영 제주대학교 방사능이용연구소 2003 연구보고 Vol.17 No.-

Personal neutron dosimetry is quite a difficult area because a neutron is always accompanied with gamma radiation, which is required of a capability for mixed field dosimetry. CaSO₄:Dy phosphor is known to have a very high sensitivity to gamma, but the neutron capture cross section of the constituents of CaSO₄:Dy are so small that the interactions between the thermal neutron and the phosphor are rare. One method to improve the neutron interaction is by introducing an impurity ion with a large thermal neutron captures cross section into the phosphor to act as a neutron target centre such as 6Li. In neutron-gamma mixed radiation fields, if two detectors for the 6Li-7Li compounds embedded CaSO₄:Dy TL pellets are used, a 6Li-compound embedded pellet can detect the neutron and gamma radiation together, and the other pellet can only detect the gamma radiation. Recently Korea Atomic Energy Research Institute (KEARI) has developed a new type of CaSO₄:Dy TL materials embedded with phosphorous (KCT-300) to detect beta and gamma radiation with a very high sensitivity[1]. This paper presents the development of CaSO₄:Dy TL pellets embedded with 6Li compound for a thermal neutron measurement, and the detection method of the neutron and gamma dose in mixed fields with CaSO₄:Dy TL pellets embedded with a 6Li compound(KCT-306) and CaSO₄:Dy TL pellets embedded with a 7Li compound(KCT-307) is introduced. The net neutron sensitivity of CaSO₄:Dy TL pellets embedded with 6Li compound developed in this study is higher than that of the TLD-600 (Harshaw Co.) dosimeter which is available in the open market.

인슐린 주사제의 고충실 액체색층기 분석

우정택,팽정렬,김성운,양인명,김진우,김영설,김광원,최영길 최신의학사 1993 最新醫學 Vol.36 No.8