Local Image Matrix Based on Poynting Vector and Its Application in RTM

Yang Hongyu,Liu Jicheng 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.6

Poynting vector is used to decompose source and receiver wavefields into different angle, then the partial images of different incident and scatter angle are computed and local imaging matrix(LIM) can be constructed. LIM composes all the angle information which is related with the geological structure, and it can be used as the base of angle-domain imaging analysis. We used LIM to reverse time migration image in angle domain and geological dip estimate. The numerical example is tested to demonstrate the computation method.

Development of CMOS Image Sensor System based on Chaos Particle Swarm

Hongyu Duan,Fengxia Yang 보안공학연구지원센터 2013 International Journal of Hybrid Information Techno Vol.6 No.6

Effects of Temperature and Additives on the Thermal Stability of Glucoamylase from Aspergillus niger

( Yang Liu ),( Zhaoli Meng ),( Ruilin Shi ),( Le Zhan ),( Wei Hu ),( Hongyu Xiang ),( Qiuhong Xie ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.1

GAM-1 and GAM-2, two themostable glucoamylases from Aspergillus niger B-30, possess different molecular masses, glycosylation, and thermal stability. In the present study, the effects of additives on the thermal inactivation of GAM-1 and GAM-2 were investigated. The half-lives of GAM-1 and GAM-2 at 70ºC were 45 and 216 min, respectively. Data obtained from fluorescence spectroscopy, circular dichroism spectroscopy, UV absorption spectroscopy, and dynamic light scattering demonstrated that during the thermal inactivation progress, combined with the loss of the helical structure and a majority of the tertiary structure, tryptophan residues were partially exposed and further led to glucoamylases aggregating. The thermal stability of GAM-1 and GAM-2 was largely improved in the presence of sorbitol and trehalose. Results from spectroscopy and Native-PAGE confirmed that sorbitol and trehalose maintained the native state of glucoamylases and prevented their thermal aggregation. The loss of hydrophobic bonding and helical structure was responsible for the decrease of glucoamylase activity. Additionally, sorbitol and trehalose significantly increased the substrate affinity and catalytic efficiency of the two glucoamylases. Our results display an insight into the thermal inactivation of glucoamylases and provide an important base for industrial applications of the thermally stable glucoamylases.

Rhombic TiO2 grown on g-C3N4 nanosheets towards fast charge transfer and enhanced Cr(VI) and NO removal

Hongyu Zhang,Ling Chen,Baogang Xu,Ping Yang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-

The strong band-to-band visible light absorption obtained by changing the bandgap of photocatalysts isdesirable but challenging for TiO2. In this paper, a mechanochemical pre-reaction and subsequent heattreatmentprocess were used to create TiO2/g-C3N4 heterojunctions. Acid-treated H2Ti3O7 nanobelts andsuperior thin g-C3N4 nanosheets (CN) were ground evenly and further heat-treated to grow rhombic TiO2in situ on the nanosheets. The heterojunctions exhibited a band gap with the absorption in visible lightregion. Heterojunction formation effective tunes the surface and electronic structures of the composite,resulting in significant decrease of bandgap. g-C3N4-based heterojunctions (5TCN) exhibited excellent H2generation (4991 lmol/g/h) and NO removal. In contrast, a TiO2-based composite (95TCN) revealed efficientphoto reduction of Cr(Ⅵ) which was 2 times of that of TiO2 sample and 22 times of that of CN. Thephotochemical reaction mechanism of TiO2 and g-C3N4-based composites was discussed with the ratio ofTiO2 and g-C3N4. The excellent performance is ascribed to single crystal rhombic TiO2 nanoparticlesgrown in situ on g-C3N4 to form well-developed heterojunctions which accelerate the carrier transfer. These results inspire the electronic structure engineering of photocatalysts to improve visible lightabsorption and provide a magic strategy for excellent photochemical activities.

Molecular profile-based recommendations for postoperative adjuvant therapy in early endometrial cancer with high-intermediate or intermediate risk: a Chinese randomized phase III trial (PROBEAT)

Yang Liu,Changkun Zhu,Hongyu Xie,Yaxia Chen,Weiguo Lv,Xing Xie,Xinyu Wang 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.2

Background: The use of molecular categorisation is shifting paradigm towards the use of molecular information to refine risk stratification in endometrial cancer (EC). To date, evidence to support molecular-guided therapies is limited to retrospective studies and secondary molecular analyses of patients receiving standard treatment. The PROBEAT study is the first randomized phase III trial to evaluate tailored adjuvant treatment based on WHO-endorsed molecular classification in Chinese EC patients. It is expected to provide a clinical decision-making tool for adjuvant treatment of patients with high-intermediate risk (HIR) or intermediate risk (IR) EC to better optimise and personalise patient care and increase relapse-free survival. Methods: The PROBEAT trial is a prospective, multicentre study led by Women’s Hospital of Zhejiang University Gynaecologic Oncology Group. Recruitment started on January 24, 2022, and 590 patients with HIR or IR endometrioid EC are expected to be recruited from 13 clinical centres in China. All tumor tissues will be classified into four molecular subtypes (POLEmut, MMRd, p53abn, or NSMP) based on WHO-endorsed molecular classification. Patients will be randomly assigned at a 2:1 ratio to either experimental arm and will receive molecular profile-based adjuvant treatment (observation in the POLEmut subgroup, vaginal brachytherapy in the MMRd or NSMP subgroup, or chemoradiotherapy in the p53abn subgroup) or to standard arm and will receive preferred adjuvant radiotherapy as recommended by the recent National Comprehensive Cancer Network guidelines version 1 (2022). The primary outcome is 3-year rates of recurrence. Secondary outcomes are relapse-free survival, overall survival, adverse events and health-related cancer-specific quality of life.

Circular RNA hsa_circ_0005556 Accelerates Gastric Cancer Progression by Sponging miR-4270 to Increase MMP19 Expression

Duo Shen,Hongyu Zhao,Peng Zeng,Jinyun Song,Yiqiong Yang,Xuefeng Gu,Qinghua Ji,Wei Zhao 대한위암학회 2020 Journal of gastric cancer Vol.20 No.3

Purpose: Circular RNAs (circRNAs) are a new class of RNA molecules whose function is largely unknown. There is a growing evidence that circRNAs play an important regulatory role in the progression of a variety of human cancers. However, the exact roles and the mechanisms of circRNAs in gastric cancer are not clear. In this study, we aimed to elucidate the mechanism of hsa_circ_0005556. Materials and Methods: Real-time quantitative polymerase chain reaction was used to detect the expression of hsa_circ_0005556, miR-4270, and matrix metalloproteinase-19 (MMP19) in gastric cancer tissues and cell lines. The expression of hsa_circ_0005556 in gastric cancer cells was silenced by lentivirus, and cell proliferation, invasion, migration, and tumorigenesis in nude mice were assessed to evaluate the function of hsa_circ_0005556 in gastric cancer. Results: The expression of hsa_circ_0005556 in gastric cancer tissues and gastric cancer cell lines was higher compared to normal controls. In vitro, the downregulation of hsa_circ_0005556 significantly inhibited proliferation, migration, and invasion of gastric cancer cells. In vivo, the downregulation of hsa_circ_0005556 suppressed tumor growth in nude mice. Conclusions: Our study shows that the hsa_circ_0005556/miR-4270/MMP19 axis is involved in proliferation, migration, and invasion of gastric cancer cells through the competing endogenous RNA (ceRNA) mechanism.

Core@shell MOFs derived Co2P/CoP@NPGC as a highly-active bifunctional electrocatalyst for ORR/OER

Weijia Gong,Hongyu Zhang,Liuyang Zhou,Ya Yang,Jiashuo Wang,Heng Liang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.106 No.-

In this study, Co2P/CoP hybrid nanoparticles (NPs) imbedded on the surface of core–shell metal–organicframeworks (MOFs) derived three-dimensional N, P co-doped graphitized carbon (Co2P/CoP@NPGC) areprepared via direct pyrolysis of P-containing MOF precursors. P dopant dosage is tailored to adjust activesites and crystalline phases of Co2P/CoP@NPGC. The active Co2P and CoP NPs and the synergistic effectfrom the Co-Nx/C and Co-P/C active sites and porous NPGC make the dominant contributions to theORR/OER. For ORR, the half-wave potential of Co2P/CoP@NPGC-1 is 0.93 V, which is superior to that ofPt/C (E1/2 = 0.875 V). As for OER, Co2P/CoP@NPGC-1 displays a lower overpotential (ƞ = 340 mV) comparedto RuO2 (ƞ = 380 mV, at 10 mA cm2). The Co2P@CoOOH heterojunction guarantees intrinsic conductivity. Furthermore, doping with N and P can modify the surface electronic structure of catalyst to lower theenergy of oxygen adsorption and dissociation, which are beneficial to enhance the ORR and OER activity. Additionally, its bifunctional activity parameter (DE) for ORR and OER is only 0.64 V, which is lower thanthat of Pt/C and RuO2 (0.76 V). Therefore, this work proposes a new sight into constructing a competitivecore–shell MOFs derived electrocatalyst for ORR/OER.

Deletion of cg1360 Affects ATP Synthase Function and Enhances Production of L-Valine in Corynebacterium glutamicum

( Xiaochen Wang ),( Hongyu Yang ),( Wei Zhou ),( Jun Liu ),( Ning Xu ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8

Bacterial ATP synthases drive ATP synthesis by a rotary mechanism, and play a vital role in physiology and cell metabolism. Corynebacterium glutamicum is well known as an industrial workhorse for amino acid production, and its ATP synthase operon contains eight structural genes and two adjacent genes, cg1360 and cg1361. So far, the physiological functions of Cg1360 (GenBank CAF19908) and Cg1361 (GenBank CAF19909) remain unclear. Here, we showed that Cg1360 was a hydrophobic protein with four transmembrane helices (TMHs), while no TMH was found in Cg1361. Deletion of cg1360, but not cg1361, led to significantly reduced cell growth using glucose and acetic acid as carbon sources, reduced F1 portions in the membrane, reduced ATP-driven proton-pumping activity and ATPase activity, suggesting that Cg1360 plays an important role in ATP synthase function. The intracellular ATP concentration in the Δcg1360 mutant was decreased to 72% of the wild type, while the NADH and NADPH levels in the Δcg1360 mutant were increased by 29% and 26%, respectively. However, the Δcg1361 mutant exhibited comparable intracellular ATP, NADH and NADPH levels with the wild-type strain. Moreover, the effect of cg1360 deletion on L-valine production was examined in the L-valine-producing V-10 strain. The final production of L-valine in the V-10-Δcg1360 mutant reached 9.2 ± 0.3 g/l in shake flasks, which was 14% higher than that of the V-10 strain. Thus, Cg1360 can be used as an effective engineering target by altering energy metabolism for the enhancement of amino acid production in C. glutamicum.

Mechanical and antibacterial properties of resin co-filled with mesoporous silica and graphene quantum dots

Lu Shuxin,Zhang Hongyu,Chai Maozhou,Yao Xiaohong,Zhang Xiangyu,Yang Yongqiang 한국탄소학회 2023 Carbon Letters Vol.33 No.2

Poor mechanical properties and bacterial infection are the main problems faced by dental restorative resins in clinical use. In this study, graphene quantum dots (GQDs) grafted with imidazole groups and mesoporous silica (MSN) are co-filled in a dental resin to impart excellent antimicrobial activity and mechanical properties to the dental resin. The higher specific surface area of GQDs and MSN results in an increased contact area with the resin matrix, which enhances the strength of the dental composite resin. The introduction of GQDs significantly improves the antimicrobial activity of the resin. The inhibition efficiency of the composite resin against Streptococcus mutans reached 99.9% with the addition of GQDs at only 0.2 wt.%. When MSN and GQDs are co-filled, MSN interferes with the release of GQDs, thus reducing the antimicrobial activity of the dental resin but improving the cyto-compatibility. By reasonably adjusting the amount of GQDs and MSN, the dental composite resin can exhibit excellent antimicrobial properties, mechanical properties and cyto-compatibility at the same time.

Functional characterization of SlGSTD3 and SlGSTD4 associated with phoxim and chlorpyrifos resistance in Spodoptera litura

Xu Li,Liu Hongyu,Yang Tao,He Chengshuai,Li Bo,Song Genmiao,Zhou Lin,Liu Runqiang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

Glutathione S-transferases (GSTs) is one of the main detoxification enzyme systems in insects and play important roles in insecticide resistance by direct metabolism, sequestration and antioxidant activity. Several GSTs genes in Spodoptera litura, a polyphagous agricultural pest, have been demonstrated to be overexpressed and involved in organophosphates and pyrethroids resistance. Previous studies have indicated the significant overexpression of two delta class GSTs genes (SlGSTd3 and SlGSTd4) in organophosphates and pyrethroids resistant populations. Here, they were heterologous expressed, and their metabolism activity and antioxidant activity were determined. Results indicated that the recombinant protein SlGSTD3 and SlGSTD4 both showed metabolism activity to phoxim and chlorpyrifos, but not to fenvalerate, cyhalothrin or beta cypermethrin. The metabolism activity of SlGSTD3 to phoxim and chlorpyrifos is higher than that of SlGSTD4. The recombinant vector of SlGSTD3 and SlGSTD4 both showed antioxidant activity after exposure to cumene hydroperoxide. Further modeling and docking analysis indicated that the 3D structure of SlGSTD3 and SlGSTD4 were well shaped for phoxim and chlorpyrifos, and the binding affinity for phoxim was stronger than that of chlorpyrifos. Our work provides evidence that SlGSTd3 and SlGSTd4 both play roles in phoxim and chlorpyrifos resistance in S. litura.