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Screening of the Antigen Epitopes of Basic Fibroblast Growth Factor by Phage Display
Xiang, Junjian,Zhong, Zhenyu,Deng, Ning,Zhong, Zhendong,Yang, Hongyu Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3
In order to investigate the epitope of basic fibroblast growth factor (bFGF) and its immunogenicity, the epitopes of bFGF were screened from the phage display library with monoclonal antibody GF22, which can neutralize the bio-activity of bFGF. By three rounds of screening, the positive phage clones with bFGF epitopes were selected, which can effectively block the bFGF to bind with GF22. Sequence analysis showed that the epitopes shared a highly conservative sequence (Leu-Pro-Pro/Leu-Gly-His-Phe/Ile-Lys). The sequence of PPGHFK was located at 22-27 of the bFGF. The specific immuno-response of mouse could be highly induced by phage clones with the epitopes. And the anti-bFGF activity induced by LPGHFK was 3 times higher than the original sequence, which showed that the mimetic peptide LPLGHIK might be used as a tumor vaccine in the prevention and treatment of tumor.
An LDPC-COFDM based High Speed Mobile Wireless Communication System
Hongyu Sun,Lijun Bi,Xiang Lu,Yinjing Guo 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.12
Coded Orthogonal Frequency Division Multiplexing (COFDM) is very well matched to the terrestrial channel, being able to cope with severe multi-path and the presence of co-channel narrowband interference. This paper presents an image and video transmission scheme based on COFDM for high speed mobile wireless communication system in which a good Rate-Compatible Low-Density Parity-Check codes structure is applied, this structure can provide a large range of supporting rates, and provide very good error performance, low decoding latency and low computational complexity at the decoder. The test experiment was made in television live transmitting to validate the designed system. The results show that our COFDM-based mobile wireless digital multimedia broadcasting system can transmit high definition image and video when it being equipped for 486Kilometre/Hour’s high speed rail, The Channel Bit Error Rate <2.0E-4 and Carrier to noise ratio(C/N) >30dB when the output power at 30dBm.
Dynamic Event-triggered Quantitative Feedback Control for Switched Affine Systems
Xiang Lu,Hongyu Sun,Xinzheng Lyu,Anhao Wen,Yinjing Guo,Gang Jing,Qunxian Zheng 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.6
This paper focuses on the design of a dynamic output feedback controller for switched affine systems under limited communication resources. Since the system states information is difficult to obtain, the dynamic output feedback switching function is considered to stabilize the switched affine system. Quantized output measurements are transmitted to the dynamic output feedback controller to reduce the communication load. In order to significantly reduce the sampling frequency, an event-triggering mechanism is introduced to detect the event periodically. By using Lyapunov stability theory and linear matrix inequality (LMI) technique, a set of dynamic output feedback gains together with a switching rule are designed assuring the global asymptotic stability of the desired equilibrium point. More specifically, the design conditions do not require that there exist a stable convex combination of the subsystems state-space matrix. Finally, a numerical example show the validity of the obtained results of this paper.
( Wei Hu ),( Jun-yan Xiang ),( Ping Kong ),( Ling Liu ),( Qiuhong Xie ),( Hongyu Xiang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.5
The single-chain variable fragment (scFv) against lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a promising molecule for its potential use in the diagnosis and immunotherapy of atherosclerosis. Producing this scFv in several milligram amounts could be the starting point for further engineering and application of the scFv. In this study, the abundant expression of the anti-LOX-1 scFv was attempted using Escherichia coli (E. coli) and Brevibacillus choshinensis (B. choshinensis). The scFv had limited soluble yield in E. coli, but it was efficiently secreted by B. choshinensis. The optimized fermentation was determined using the Plackett-Burman screening design and response surface methodology, under which the yield reached up to 1.5 g/l in a 5-L fermentor. Moreover, the properties of the scFvs obtained from the two expression systems were different. The antigen affinity, transition temperature, and particle diameter size were 1.01E-07 M, 55.2 ± 0.3℃, and 9.388 nm for the scFv expressed by B. choshinensis, and 4.53E-07 M, 52.5 ± 0.3℃, and 13.54 nm for the scFv expressed by E. coli. This study established an efficient scale-up production methodology for the anti-LOX-1 scFv, which will boost its use in LOX-1-based therapy.
Effects of Temperature and Additives on the Thermal Stability of Glucoamylase from Aspergillus niger
( Yang Liu ),( Zhaoli Meng ),( Ruilin Shi ),( Le Zhan ),( Wei Hu ),( Hongyu Xiang ),( Qiuhong Xie ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.1
GAM-1 and GAM-2, two themostable glucoamylases from Aspergillus niger B-30, possess different molecular masses, glycosylation, and thermal stability. In the present study, the effects of additives on the thermal inactivation of GAM-1 and GAM-2 were investigated. The half-lives of GAM-1 and GAM-2 at 70ºC were 45 and 216 min, respectively. Data obtained from fluorescence spectroscopy, circular dichroism spectroscopy, UV absorption spectroscopy, and dynamic light scattering demonstrated that during the thermal inactivation progress, combined with the loss of the helical structure and a majority of the tertiary structure, tryptophan residues were partially exposed and further led to glucoamylases aggregating. The thermal stability of GAM-1 and GAM-2 was largely improved in the presence of sorbitol and trehalose. Results from spectroscopy and Native-PAGE confirmed that sorbitol and trehalose maintained the native state of glucoamylases and prevented their thermal aggregation. The loss of hydrophobic bonding and helical structure was responsible for the decrease of glucoamylase activity. Additionally, sorbitol and trehalose significantly increased the substrate affinity and catalytic efficiency of the two glucoamylases. Our results display an insight into the thermal inactivation of glucoamylases and provide an important base for industrial applications of the thermally stable glucoamylases.
( Yong-fei Zhou ),( Jiao-jiao Nie ),( Chao Shi ),( Ke Ning ),( Yu-feng Cao ),( Yanbo Xie ),( Hongyu Xiang ),( Qiuhong Xie ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10
COVID-19 is an emerging disease that poses a severe threat to global public health. As such, there is an urgent demand for vaccines against SARS-CoV-2, the virus that causes COVID-19. Here, we describe a virus-like nanoparticle candidate vaccine against SARS-CoV-2 produced by an E. coli expression system. The fusion protein of a truncated ORF2-encoded protein of aa 439~608 (p170) from hepatitis E virus CCJD-517 and the receptor-binding domain of the spike protein from SARSCoV-2 were expressed, purified and characterized. The antigenicity and immunogenicity of p170-RBD were evaluated in vitro and in Kunming mice. Our investigation revealed that p170-RBD selfassembled into approximately 24 nm virus-like particles, which could bind to serum from vaccinated people (p < 0.001) and receptors on cells. Immunization with p170-RBD induced the titer of IgG antibody vaccine increased from 14 days post-immunization and was significantly enhanced after a booster immunization at 28 dpi, ultimately reaching a peak level on 42 dpi with a titer of 4.97 log10. Pseudovirus neutralization tests showed that the candidate vaccine induced a strong neutralizing antibody response in mice. In this research, we demonstrated that p170-RBD possesses strong antigenicity and immunogenicity and could be a potential candidate for use in future SARS-CoV-2 vaccine development.