Application of Wireless Electromagnetic Resonance Technology to Rotary Transformer Used in Ultrasonic Spindle

Yuki INOUE,Yafei GAO,Masahito SHOYAMA,Hideaki FUJITA 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

In order to develop a new style drill for the factories, the ultrasonic spindle was designed. Rotary transformer was introduced to solve the design problems existing in the specification and structure. However, rotary transformer’s voltage conversion ratio is markedly lower than the inductance ratio, it cannot transmit sufficient electric power to the ultrasonic element. Then, this problem is being solved by using electromagnetism resonance technology in this paper.

Summarizing the Differences in Chinese-Vietnamese Bilingual News

Wu, Jinjuan,Yu, Zhengtao,Liu, Shulong,Zhang, Yafei,Gao, Shengxiang Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.6

Summarizing the differences in Chinese-Vietnamese bilingual news plays an important supporting role in the comparative analysis of news views between China and Vietnam. Aiming at cross-language problems in the analysis of the differences between Chinese and Vietnamese bilingual news, we propose a new method of summarizing the differences based on an undirected graph model. The method extracts elements to represent the sentences, and builds a bridge between different languages based on Wikipedia's multilingual concept description page. Firstly, we calculate the similarity between Chinese and Vietnamese news sentences, and filter the bilingual sentences accordingly. Then we use the filtered sentences as nodes and the similarity grade as the weight of the edge to construct an undirected graph model. Finally, combining the random walk algorithm, the weight of the node is calculated according to the weight of the edge, and sentences with highest weight can be extracted as the difference summary. The experiment results show that our proposed approach achieved the highest score of 0.1837 on the annotated test set, which outperforms the state-of-the-art summarization models.

Summarizing the Differences in Chinese-Vietnamese Bilingual News

Jinjuan Wu,Zhengtao Yu,Shulong Liu,Yafei Zhang,Shengxiang Gao 한국정보처리학회 2019 Journal of information processing systems Vol.15 No.6

Summarizing the differences in Chinese-Vietnamese bilingual news plays an important supporting role in thecomparative analysis of news views between China and Vietnam. Aiming at cross-language problems in theanalysis of the differences between Chinese and Vietnamese bilingual news, we propose a new method ofsummarizing the differences based on an undirected graph model. The method extracts elements to representthe sentences, and builds a bridge between different languages based on Wikipedia’s multilingual conceptdescription page. Firstly, we calculate the similarity between Chinese and Vietnamese news sentences, andfilter the bilingual sentences accordingly. Then we use the filtered sentences as nodes and the similarity gradeas the weight of the edge to construct an undirected graph model. Finally, combining the random walkalgorithm, the weight of the node is calculated according to the weight of the edge, and sentences with highestweight can be extracted as the difference summary. The experiment results show that our proposed approachachieved the highest score of 0.1837 on the annotated test set, which outperforms the state-of-the-artsummarization models.

Relationship among porcine lncRNA TCONS_00010987, miR-323, and leptin receptor based on dual luciferase reporter gene assays and expression patterns

Ding, Yueyun,Qian, Li,Wang, Li,Wu, Chaodong,Li, DengTao,Zhang, Xiaodong,Yin, Zongjun,Wang, Yuanlang,Zhang, Wei,Wu, Xudong,Ding, Jian,Yang, Min,Zhang, Liang,Shang, Jinnan,Wang, Chonglong,Gao, Yafei Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.2

Objective: Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3'-UTR fragments were generated and cloned into pmiRRB-REPORT^TM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wild-type LEPR-3'-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3'-UTR (p>0.05 for both). Backfat expression levels of TCONS_00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

Relationship between porcine miR-20a and its putative target low-density lipoprotein receptor based on dual luciferase reporter gene assays

Yueyun Ding,Shujiao Zhu,Chaodong Wu,Li Qian,DengTao Li,Li Wang,Yuanlang Wang,Wei Zhang,Min Yang,Jian Ding,Xudong Wu,Xiao-Dong Zhang,Yafei Gao,Zongjun Yin 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.7

Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3′-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3′-UTR and pGL3 Control LDLR mutant-3′-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine pre-miR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3′-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR-3′-UTR-driven (p<0.01), but not mutant LDLR-3′-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = –0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3′-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.