Acute Liver Failure Associated with Occupational Exposure to Tetrachloroethylene

Chuan Shen,Cai-Yan Zhao,Fang Liu,Ya-Dong Wang,Wei Wang 대한의학회 2011 Journal of Korean medical science Vol.26 No.1

Tetrachloroethylene is a chlorinated solvent that is primarily used in dry cleaning and degreasing operations. Although the hepatotoxicity caused by tetrachloroethylene has been well documented in literature, it is rarely considered as a cause of acute liver failure. We report a case of a 39-yr-old man who was admitted to our hospital for acute liver failure due to tetrachloroethylene exposure. Histological examination of the liver revealed massive hepatic necrosis, prominently, in zone 3 of the hepatic lobules. The patient underwent supportive treatment along with 3 sessions of plasmapheresis, and consequently, he presented a favorable outcome. Repeat liver biopsy performed 6 months after the patient’s discharge showed architectural distortion with postnecrotic cirrhosis. Physicians should be aware of the possibility of acute liver failure induced by tetrachloroethylene. Early plasmapheresis can be effective for individuals with sufficient capacity for hepatocyte regeneration.

Pose Measuring and Aligning of a Micro Glass Tube and a Hole on the Micro Sphere

Fu-Dong Li,De Xu,Zheng-Tao Zhang,Ya-Li Shi,Fei Shen 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

To ensure high uniformity of the inner space of the hollow sphere after assembly, the glass tube must be inserted into the hole on thesphere towards the center of the sphere. This paper deals with the pose measuring of the hole on the sphere and the glass tube andpose aligning of the two objects. The sphere is 500 mm in diameter with a 20 mm hole on it, and the glass tube is 17 mm in diameter. Novel pose measuring method for the hole on the sphere and the glass tube is developed, using two microscopic cameras, each oneacquires a projection vector of the object to be measured. A plane containing the optical axis of the microscopic camera and the posevector of the object is determined, with the microscopic camera calibrated in advance. Pose vector of the object to be measured canbe calculated by the intersection of the two planes acquired by the two microscopic cameras. Error analysis of the pose measuringmethod is conducted and experimental results were consistent with analytical results. Less than 0.7o pose aligning error is achievedusing the proposed pose measuring method and pose aligning method.

Step-size adaptive parametric level set method for structural topology optimization

Chen-Dong Yang,Jian-Hu Feng,Ya-Dong Shen 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10

In the structural topology optimization (STO), the step-size of the parametric level set method (PLSM) using the explicit scheme must satisfy the Courant-Friedrichs-Lewy (CFL) condition to ensure numerical stability. However, much larger step-sizes are arbitrarily used to speed up the convergence. For this reason, a narrowband in the velocity field is defined, and the step-size adaptive parametric level set method (SAPLSM) is proposed, which multiplies different step-sizes for the velocity of different nodes. The SAPLSM satisfies the CFL condition not only on the narrowband, but also on the entire design domain. Furthermore, a narrowband annealing (NA) scheme based on “annealing” is proposed to dynamically adjust the maximum step-size during the iterations. Numerical experimental results of several benchmark problems in two-dimensional minimum compliance show that: (1) The SAPLSM is more stable than PLSM under large step-sizes and complex problems. (2) The NA scheme not only accelerates the convergence of SAPLSM but also alleviates mesh dependence.

Long-term surgical outcomes of liver resection for hepatocellular carcinoma in patients with hepatitis B virus and hepatitis C virus co-infection: A multicenter observational study

Hang-Dong JIA,Lei LIANG,Chao LI,Han WU,Hong WANG,Ying-Jian LIANG,Ya-Hao ZHOU,Wei-Min GU,Xin-Ping FAN,Wan Yee LAU,Timothy M. PAWLIK,Yong-Kang DIAO,Qiu-Ran XU,Feng SHEN,Cheng-Wu ZHANG,Dong-Sheng HUANG,T 한국간담췌외과학회 2021 Annals of hepato-biliary-pancreatic surgery Vol.25 No.-

Effect of all-trans retinoic acid on casein and fatty acid synthesis in MAC-T cells

Liao, Xian-Dong,Zhou, Chang-Hai,Zhang, Jing,Shen, Jing-Lin,Wang, Ya-Jing,Jin, Yong-Cheng,Li, Sheng-Li Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: Caseins and fatty acids of milk are synthesized and secreted by the epithelial cells of the mammary gland. All-trans retinoic acid (ATRA), an active metabolite of vitamin A, has been shown to promote mammary development. This study was conducted to determine the effect of ATRA on casein synthesis and fatty acid composition in MAC-T cells. Methods: MAC-T cells were allowed to differentiate for 4 d, treated with ATRA (0, 1.0, 1.5, and 2.0 μM), and incubated for 3 d. We analyzed the fatty acid composition, the mRNA expression of casein and fatty acid synthesis-related genes, and the phosphorylation of casein synthesis-related proteins of MAC-T cells by gas chromatography, quantitative polymerase chain reaction, and western blotting, respectively. Results: In MAC-T cells, ATRA increased the mRNA levels of α_S1-casein and β-casein, janus kinase 2 (JAK2) and E74-like factor 5 of the signal transducer and activator of transcription 5 β (STAT5-β) pathway, ribosomal protein S6 kinase beta-1 (S6K1) and eukaryotic translation initiation factor 4E binding protein 1 of the mammalian target of rapamycin (mTOR) pathway, inhibited the mRNA expression of phosphoinositide 3-kinase and eukaryotic initiation factor 4E of the mTOR pathway, and promoted the phosphorylation of STAT5-β and S6K1 proteins. Additionally, ATRA increased the de novo synthesis of fatty acids, reduced the content of long-chain fatty acids, the ratio of monounsaturated fatty acids to saturated fatty acids (SFA), the ratio of polyunsaturated fatty acids (PUFA) to SFA, and the ratio of ω-6 to ω-3 PUFA. The mRNA levels of acetyl-CoA carboxylase 1, fatty acid synthase, lipoprotein lipase, stearoyl-CoA desaturase, peroxisome proliferator-activated receptor gamma, and sterol regulatory element-binding protein 1 (SREBP1) were enhanced by ATRA. Conclusion: ATRA promotes the synthesis of casein by regulating JAK2/STAT5 pathway and downstream mTOR signaling pathway, and it improves the fatty acid composition of MAC-T cells by regulating SREBP1-related genes.

Prognostic Significance of GSTP1, XRCC1 and XRCC3 Polymorphisms in Non-small Cell Lung Cancer Patients

Ke, Hong-Gang,Li, Jun,Shen, Yi,You, Qing-Sheng,Yan, Yu,Dong, Han-Xuan,Liu, Jun-Hua,Shen, Zhen-Ya Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Aim: Individual differences in chemosensitivity and clinical outcome in non-small cell lung cancer (NSCLC) patients treatment with platinum-based chemotherapy may be due to genetic factors. Our study aimed to investigate the prognostic role of GSTP1, XRCC1 and XRCC3 in NSCLC patients treated with chemotherapy. Methods: A total of 460 cases were consecutively selected from The Affiliated Hospital of Nantong University between Jan. 2003 to Nov. 2006, and all were followed-up until Nov. 2011. Genotyping of GSTP1 Ile105Val, XRCC1 Arg194Trp, XRCC1 Arg399Gln and XRCC3 Thr241Met was conducted by duplex polymerase-chain-reaction with confronting-two-pair primer methods. Results: Patients with GSTP Val/Val exhibited a shorter survival time, and had a 1.89 fold greater risk of death than did those with the IIe/IIe genotype. For XRCC1 Arg194Trp, the variant genotype Trp/Trp was significantly associated with a decreased risk of death from NSCLC when compared with the Arg/Arg. Individuals carrying XRCC1 399Gln/Gln genotype had a longer survival time, with a lowered risk of death from NSCLC. Conclusion: This study indicated that GSTP1 Ile105Val, XRCC1 Arg194Trp and XRCC1Arg399Gln genes have a role in modifying the effect of platinum-based chemotherapy for NSCLC patients in a Chinese population. Our findings provide information for therapeutic decisions for individualized therapy in NSCLC cases.

Preferential Induction of CYP1A1 over CYP1B1 in Human Breast Cancer MCF-7 Cells after Exposure to Berberine

Wen, Chun-Jie,Wu, Lan-Xiang,Fu, Li-Juan,Shen, Dong-Ya,Zhang, Xue,Zhang, Yi-Wen,Yu, Jing,Zhou, Hong-Hao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Estrogens are considered the major breast cancer risk factor, and the carcinogenic potential of estrogens might be attributed to DNA modification caused by derivatives formed during metabolism. $17{\beta}$-estradiol ($E_2$), the main steroidal estrogen present in women, is metabolized via two major pathways: formation of 2-hydroxyestradiol (2-OH $E_2$) and 4-hydroxyestradiol ($4-OH\;E_2$) through the action of cytochrome P450 (CYP) 1A1 and 1B1, respectively. Previous reports suggested that $2-OH\;E_2$ has putative protective effects, while $4-OH\;E_2$ is genotoxic and has potent carcinogenic activity. Thus, the ratio of $2-OH\;E_2/4-OH\;E_2$ is a critical determinant of the toxicity of $E_2$ in mammary cells. In the present study, we investigated the effects of berberine on the expression profile of the estrogen metabolizing enzymes CYP1A1 and CYP1B1 in breast cancer MCF-7 cells. Berberine treatment produced significant induction of both forms at the level of mRNA expression, but with increased doses produced 16~ to 52~fold greater induction of CYP1A1 mRNA over CYP1B1 mRNA. Furthermore, berberine dramatically increased CYP1A1 protein levels but did not influence CYP1B1 protein levels in MCF-7 cells. In conclusion, we present the first report to show that berberine may provide protection against breast cancer by altering the ratio of CYP1A1/CYP1B1, could redirect $E_2$ metabolism in a more protective pathway in breast cancer MCF-7 cells.