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      • KCI등재

        Predicting Media Credibility in China: The Influence of Weibo Use

        Shen, Fei,Zhang, Hongzhong Center for Asian Public Opinion ResearchCollaborat 2014 Asian journal for public opinion research Vol.1 No.4

        A telephone survey was conducted in a metropolitan city in 2012 to examine people's credibility ratings of different media outlets, in particular, Weibo - one of the most popular social media platforms in China. Our findings suggest: First, people place more trust in traditional news media than in online sources by a significant margin. Second, demographic influences on media trust seem to be minimal. Only age and gender were related to some credibility measures. Third, Weibo use was not related to one's credibility perception toward traditional media but interestingly, Weibo use showed different impacts on people's evaluation of Weibo's credibility. Commenting frequency was negatively related to one's trust in Weibo, while retweeting frequency was positively related to one's trust in Weibo.

      • KCI등재

        An Automatic Assembly Control Method for Peg and Hole based on Multidimensional Micro Forces and Torques

        Fei Shen,Zheng-Tao Zhang,De Xu,Juan Zhang,Wenrong Wu 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.8

        In this paper, an automatic assembly method for peg and hole based on multidimensional micro forces and torques is developed for satisfying the accurate and lossless assembly requirements. The relationship between the forces and torques from the force sensor and the attitudes deviation of peg and hole is discussed. Moreover, a novel method based on active constraint state for estimating the deviation angles between the peg and the hole through the toques exerted on the sensor is proposed. To achieve automated insertion and minimize the contact forces and torques in the meanwhile during the insertion process, a control strategy for controlling contact forces and torques is proposed by eliminating the position and attitudes deviation interactively. Related assembly experiments are conducted to verify the effectiveness of the proposed approaches.

      • KCI등재

        말단 수산화기를 가진 폴리락타이드와 이미다졸로 블록된 이소시아네이트를 이용한 폴리우레탄 바이오접착제의 합성 및 물성 평가

        Teng Fei Shen,Ying Juan Sun,Chun Feng Sun,Man Geng Lu 한국고분자학회 2013 폴리머 Vol.37 No.2

        A series of novel imidazole-blocked diisocyanate bioadhesives (IBAs) were synthesized from reaction of toluene 2, 4-diisocyanate (TDI), isophorone diisocyanate (IPDI), hydroxyl-terminated polylactide (HO-PLA-OH), 1,1,1-trimethylolpropane (TMP), and imidazole. Synthesis of IBAs was confirmed by Fourier transform infrared spectroscopy (FTIR) and gel permeation chromatography (GPC). Differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA) revealed that the TDI-based IBA had lower thermal dissociation temperature and a faster deblocking rate than IBA based on IPDI. Hydroxyl-terminated polyurethane (HPU) was introduced to study the adhesive effect of the synthesized IBAs. Improvement on elastic modulus, tensile strength and water resistance of IBA-modified HPU in comparison with neat HPU suggested the good adhesive effect of IBA due to the strong chemical reaction between released NCO groups from IBA and hydroxyl groups from HPU.

      • Enhancing cationic lipid-mediated tranfection efficiency of NT2/D1 cells

        심안비 ( Yan Fei Shen ),류명걸 ( Ming Jie Liu ),유맹루 ( Meng Lu Liu ),유양 ( Yang Yu ),이연식 ( Youn Sik Lee ),홍성출 ( Seong Tshool Hong ) 전북대학교 의과학연구소 2007 全北醫大論文集 Vol.31 No.2

        Cationic lipids, such as lipofectamine series, are the most well developed nonviral vectors that widely used to transfect nucleic acids into eukaryotic cells. Although cationic lipids are known as safer carriers, their transfection efficiency remains low in certain cell lines, such as NT2/D1. In this study, we developed an efficient method which mix the lipofectamine 2000/plasmid pEGFP-N1 lipoplex with trypsin treated NT2/D1 cells in small volume for 1 minute to improve the transfection efficiency. Transfection efficiency of NT2/D1 was significantly increased by this method up to 90% without visible toxicity and expression of transfected pEGFP-N1 plasmid was 2.6-fold increased than using conventional method.

      • KCI등재

        Comparative Study on Single Pulse and Dual Pulses Variable Width Control Strategies for High-Speed PM BLDC Motor Drive

        Jian-Xin Shen,Xue-Fei Qin,Wei-Zhong Fei,Yun-Chong Wang 한국자기학회 2019 Journal of Magnetics Vol.24 No.2

        Pulse width modulation (PWM) of voltage source inverter (VSI) is common for speed regulation of permanent magnet (PM) brushless direct-current (BLDC) motor drives. However, when the motor runs at high speed, the conventional PWM technique may become unfeasible due to the low carrier ratio of inverter, which makes the motor current distorted and the motor core loss increased significantly. In this paper, two control strategies, namely single pulse variable width (SPVW) and dual pulses variable width (DPVW), are introduced for the high-speed PM BLDC motor drives, and are comprehensively investigated. Finite element method (FEM) is employed to evaluate and compare the drive system performance when using the conventional PWM, and the proposed SPVW and DPVW, respectively. Influence of the control techniques, especially on the loss distribution, is revealed.

      • KCI등재

        SRSF7 is a promising prognostic biomarker in hepatocellular carcinoma and is associated with immune infiltration

        Shen Wei,Yuan Lebin,Cheng Fei,Wu Zhao,Li Xiaodong 한국유전학회 2024 Genes & Genomics Vol.46 No.1

        Background Previous studies indicate that the splicing process, regulated by the cellular machinery of tumors (spliceosome), undergoes alterations, leading to oncogenic splicing events associated with the progression of tumors towards aggressiveness. However, the role of serine/arginine-rich splicing factor 7 (SRSF7) in hepatocellular carcinoma (HCC) and the tumor microenvironment (TME) remains unclear. Methods This study was aimed to explore the role and clinical significance of SRSF7 in HCC. By conducting functional analysis and gene set enrichment analysis, it was discovered that SRSF7 contributes to multiple pathways associated with immune response and tumor advancement. Further experiments verified that silencing of SRSF7 obviously inhibits progression of HCC. Results Aberrant expression of SRSF7, which were referred as an independent prognostic risk factor, effectively predicts the prognosis of patients with HCC. Functional and gene enrichment analyses revealed that SRSF7 is linked with multiple immune and tumor progression-related pathways, including the B cell receptor signaling pathway, positive regulation of leukocyte and immunoglobulin receptor binding cell activation, nuclear division, membrane invagination, cell cycle, as well as mTOR signaling pathway. Furthermore, increased SRSF7 expression was associated with tumor-infiltrating inflammatory cells (CD4+, monocytes/macrophages, CD8 + and endothelial). Additionally, multiple immune checkpoint genes were markedly positively related to SRSF7. The efficiency of SRSF7 in predicting immunomodulator and chemokine responses were also assessed in microenvironment. Moreover, in vitro analyses demonstrated that knockdown of SRSF7 suppressed the malignant evolution of HCC possibly by deactivating the PI3K/AKT/mTOR signaling. Conclusion The role of SRSF7 in the tumor microenvironment has been successfully assessed. It may be a valid bio-index for predicting the HCC prognosis, thereby guiding individualized immunotherapy for cancer. Background Previous studies indicate that the splicing process, regulated by the cellular machinery of tumors (spliceosome), undergoes alterations, leading to oncogenic splicing events associated with the progression of tumors towards aggressiveness. However, the role of serine/arginine-rich splicing factor 7 (SRSF7) in hepatocellular carcinoma (HCC) and the tumor microenvironment (TME) remains unclear. Methods This study was aimed to explore the role and clinical significance of SRSF7 in HCC. By conducting functional analysis and gene set enrichment analysis, it was discovered that SRSF7 contributes to multiple pathways associated with immune response and tumor advancement. Further experiments verified that silencing of SRSF7 obviously inhibits progression of HCC. Results Aberrant expression of SRSF7, which were referred as an independent prognostic risk factor, effectively predicts the prognosis of patients with HCC. Functional and gene enrichment analyses revealed that SRSF7 is linked with multiple immune and tumor progression-related pathways, including the B cell receptor signaling pathway, positive regulation of leukocyte and immunoglobulin receptor binding cell activation, nuclear division, membrane invagination, cell cycle, as well as mTOR signaling pathway. Furthermore, increased SRSF7 expression was associated with tumor-infiltrating inflammatory cells (CD4+, monocytes/macrophages, CD8 + and endothelial). Additionally, multiple immune checkpoint genes were markedly positively related to SRSF7. The efficiency of SRSF7 in predicting immunomodulator and chemokine responses were also assessed in microenvironment. Moreover, in vitro analyses demonstrated that knockdown of SRSF7 suppressed the malignant evolution of HCC possibly by deactivating the PI3K/AKT/mTOR signaling. Conclusion The role of SRSF7 in the tumor microenvironment has been successfully assessed. It may be a valid bio-index for predicting the HCC prognosis, thereby guiding individualized immunotherapy for cancer.

      • SCISCIESCOPUS

        Risk evaluation of biochars produced from Cd-contaminated rice straw and optimization of its production for Cd removal

        Shen, Zhengtao,Fan, Xiaoliang,Hou, Deyi,Jin, Fei,O'Connor, David,Tsang, Daniel C.W.,Ok, Yong Sik,Alessi, Daniel S. Pergamon Press 2019 Chemosphere Vol. No.

        <P><B>Abstract</B></P> <P>Based on the “waste-treat-waste” concept, biochars were produced from cadmium (Cd)-contaminated rice straw (CRSBs) at 300, 500, and 700 °C (CRSB300, CRSB500, and CRSB700). The risks of the Cd remaining in CRSBs were evaluated and the optimal biochar pyrolysis temperature for Cd removal was investigated. It was observed that 41% of the total Cd in the raw rice straw was exchangeable, which may pose significant risks to crops and humans. Pyrolyzing at 300 °C did not significantly alter the Cd fractions, while the exchangeable fraction of Cd greatly dropped to 5.79% at 500 °C and further to 2.12% at 700 °C. Increasing the highest pyrolysis temperature resulted in CRSBs with higher pH values, greater surface area, and smaller pore sizes, thus providing more rapid and efficient removal of Cd from aqueous solutions. For Cd removal tests, increasing pyrolysis temperature (300–700 °C) increased the total (24.8–55.1 mg/g) and non-exchangeable (18.9–52.8 mg/g) Cd concentrations immobilized on the CRSBs and significantly decreased the exchangeable Cd fraction (23.7%–4.85%). It is suggested based on the study from aqueous solutions that CRSB700 was the most suitable for the remediation of Cd contaminated soil on site due to the lowest risks of remained Cd from feedstock, fastest and highest Cd removal, and most stable immobilization of Cd.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 41% of Cd in raw rice straw was exchangeable, posing great environmental risks. </LI> <LI> Pyrolyzing at 300 °C did not significantly alter Cd fractions remained in biochar. </LI> <LI> Exchangeable fraction of Cd dropped to 5.79% at 500 °C and to 2.12% at 700 °C. </LI> <LI> Increasing temperature decreased exchangeable Cd fraction immobilized on biochar. </LI> <LI> CRSB700 has the fastest and highest Cd removal, and most stable Cd immobilization. </LI> </UL> </P>

      • KCI등재

        Characterization and profiling of MicroRNAs in posterior silk gland of the silkworm (Bombyx mori)

        Fei Song,Xin Wang,Chen Chen,Yangyang Fan,Shunming Tang,Jinshan Huang,Xijie Guo,Xingjia Shen 한국유전학회 2015 Genes & Genomics Vol.37 No.8

        MicroRNAs (miRNAs) regulate expression of genes at post-transcriptional level by binding on complementary sequences of target mRNAs and play multiple roles in biological processes. To investigate the differential expression of miRNAs in posterior silk gland (PSG) of silkworm (Bombyx mori) in different periods and regulation of miRNAs on the expression of fibroin genes, Solexa sequencing technology was used to detect miRNAs in PSGs of fourth-instar day-2 larvae and fifth-instar day-3 larvae, respectively. As a result, 466 previously reported miRNAs, and 35 novel miRNAs were detected, and 499 of these detected miRNAs are predicted to target 13,383 genes by target prediction softwares. Additionally, 29 miRNAs expressed differently between the PSG of fourthinstar day-2 larvae and fifth-instar day-3 larvae were found, and the differential expression of these miRNAs may play an important role in the expression of fibroin genes.

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