MicroRNA-590-5p Regulates Proliferation and Invasion in Human Hepatocellular Carcinoma Cells by Targeting TGF-beta RII

Xiaofeng Jiang,De Chen,Guangyang Xiang,Yezeng Wang,Lei Zhang,Xuewei Yang,Liangqi Cao,Heping Peng,Ping Xue 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.6

MicroRNAs (miRNAs) are regulatory small non-coding RNAs that can regulate gene expression by binding to gene elements, such as the gene promotor 5UTR, mainly in the 3UTR of mRNA. One miRNA targets many mRNAs, which can be regulated by many miRNAs, leading to a complex metabolic network. In our study, we found that the expression level of miR-590-5p is higher in the human hepatocellular carcinoma cell line HepG2 than in the normal hepatocellular cell line L02. Downregulation of miR-590-5p inhibited proliferation and invasion of hepatocellular carcinoma cells (HCCs). We also showed that expression of TGF-beta RII, which has been regarded as a regulator of tumor proliferation, invasion, and migration in hepatocellular carcinoma, is regulated by miRNA-590-5p. In addition, miR-590-5p downregulated the expression of TGF- beta RII by targeting the 3UTR of mRNA. We also found that downregulation of miR-590-5p was associated with an elevation of TGF-beta RII and inhibition of proliferation and invasion in HepG2 cells. Furthermore, overex-pression of miR-590-5p was associated with upregulation of TGF-beta RII and could promote proliferation and invasion in L02 cells. In conclusion, we determined that TGF-beta RII is a novel target of miRNA-590-5p. Thus, the role of TGF-beta RII in regulating proliferation and invasion of human HCCs is controlled by miR-590-5p. In other words, miR-590-5p promotes proliferation and invasion in human HCCs by directly targeting TGF-beta RII.

Correlations of Fecal Bacterial Communities with Age and Living Region for the Elderly Living in Bama, Guangxi, China

Liang Zhao,Xuewei Qiao,Jun Zhu,Xiaoying Zhang,Jingli Jiang,Yanling Hao,Fazheng Ren 한국미생물학회 2011 The journal of microbiology Vol.49 No.2

Bama County (Guangxi, China) is famous for its longevous population. In this study, intestinal microflora of 17 healthy elderly subjects of different ages and from different regions (rural and urban) in Bama,were analyzed by denaturing gradient gel electrophoresis (DGGE). Significant effects of age and living region on the whole intestinal bacterial communities were observed by redundancy analysis (RDA). A total of 11 bacterial strains that were correlated with age and living region were identified using a t-value biplot combined with band sequencing. Four bacterial strains were correlated with both age and living region of the elderly in Bama. Two Bacteroides strains and one Ruminococcaceae strain were abundant in the rural, younger elderly; conversely, one Desulfovibrio strain was high in the urban, older elderly. Another Bacteroidetes strain was only correlated with the participant’s age, and its abundance increased with the age of the elderly. The richness of one Clostridium sordellii strain, which was only correlated with the elderly living region, was high in the urban elderly. The study also found five other novel bacterial strains that were correlated with the age or living region of the elderly in Bama. These results expand our understanding of age- and region-effects on the intestinal microflora of the elderly and raise the possibility of developing probiotics originating from centenarians.

Lactobacillus salivarius REN Counteracted Unfavorable 4-Nitroquinoline-1-Oxide-Induced Changes in Colonic Microflora of Rats

Ming Zhang,Xuewei Qiao,Liang Zhao,Lu Jiang,Fazheng Ren 한국미생물학회 2011 The journal of microbiology Vol.49 No.6

Probiotics and carcinogens both have a significant effect on the microfloral composition of the human intestine. The objective of this study was to investigate the impact of an important carcinogen, 4-Nitroquinoline-1- Oxide on colonic microflora and the efficacy of the probiotic Lactobacillus salivarius REN as an agent of counteracting these effects. Using denaturing gradient gel electrophoresis (DGGE) combined with redundancy analysis, we demonstrated that both 4-Nitroquinoline-1-Oxide and L. salivarius REN significantly altered the bacterial communities of rat colons. A total of 27 bacterial strains were identified as being affected by treatment with 4-Nitroquinoline-1-Oxide or L. salivarius REN using a t-value biplot combined with band sequencing. 4-Nitroquinoline-1-Oxide treatment increased the abundance of two potential pathogens (one Helicobacter strain and one Desulfovibrio strain), as well as reducing the abundance of two potentially beneficial strains (one Ruminococcaceae strain and one Rumen bacteria). The Helicobacter strain was initally detected in carcinogen-treated rat intestinal microflora, but L. salivarius REN treatment effectively suppressed the growth of the Helicobacter strain. These results suggested that L. salivarius REN may be a potential probiotic, efficiently acting against the initial infection with, and the growth of pathogenic bacteria.

Cotton GhKCH2, a Plant-specific Kinesin, is Low-affinitive and Nucleotide-independent as Binding to Microtubule

Xu, Tao,Sun, Xuewei,Jiang, Shiling,Ren, Dongtao,Liu, Guoqin Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5

Kinesin is an ATP-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. The kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. Animal kinesins have been well characterized. In contrast, plant kinesins have not yet to be characterized adequately. Here, a novel plant-specific kinesin gene, GhKCH2, has been cloned from cotton (Gossypium hirsutum) fibers and biochemically identified by prokaryotic expression, affinity purification, ATPase activity assay and microtubule-binding analysis. The putative motor domain of GhKCH2, $M_{396-734}$ corresponding to amino acids Q396-N734 was fused with 6$\times$His-tag, soluble-expressed in E. coli and affinity-purified in a large amount. The biochemical analysis demonstrated that the basal ATPase activity of $M_{396-734}$ is not activated by $Ca^{2+}$, but stimulated 30-fold max by microtubules. The enzymatic activation is microtubule-concentration-dependent, and the concentration of microtubules that corresponds to half-maximum activation was about 11 ${\mu}M$, much higher than that of other kinesins reported. The cosedimentation assay indicated that $M_{396-734}$ could bind to microtubules in vitro whenever the nucleotide AMP-PNP is present or absent. As a plant-specific microtubule-dependent kinesin with a lower microtubule-affinity and a nucleotide-independent microtubule-binding ability, cotton GhKCH2 might be involved in the function of microtubules during the deposition of cellulose microfibrils in fibers or the formation of cell wall.

Switching Motion Control of Aircraft Skin Inspection Robot Using Backstepping Scheme and Nussbaum Disturbance Observer

Congqing Wang,Junjun Jiang,Xuewei Wu,Linfeng Wu 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.6

In this paper, a backstepping control using switching strategy is proposed for the motion control of the aircraft skin inspection robot with double frame in the presence of external disturbances. The inspection robot equipped with a CCD camera and an ultrasonic sensor can alternately adsorb and move on the aircraft surface. The influence of the external disturbances is obvious in the switching motion. The disturbances are efficiently estimated using a Nussbaum disturbance observer (NDO), and the disturbance observer errors are uniformly ultimately bounded. Then, the backstepping control method is used to design the motion controller. The stability of the closed-loop robot system is proved by Lyapunov analysis through the average dwell time method .The tracking errors and the disturbance observer errors are semi-globally uniformly bounded using the proposed control schemewith NDO,and NDO is compared with Super-twisting disturbance observer. Finally, simulation results illustrate that the proposed control scheme with NDO can achieve satisfactory tracking performance under the external disturbance.

Genome-wide analysis and environmental response profiling of dirigent family genes in rice (Oryza sativa)

Rongjun Chen,Yongrong Liao,Shengbin Liu,Yunyun Jiang,Changqiong Hu,Xuewei Zhang,Xufeng Cao,Zhengjun Xu,Xiaoling Gao,Lihua Li,Jianqing Zhu 한국유전학회 2017 Genes & Genomics Vol.39 No.1

Dirigent (DIR) and DIR-like family genes were involved in lignification or in the response to pathogen infection and abiotic stress in plants. Little is known to us about how rice DIR genes respond to adverse conditions. In this study, we reported genome-wide analysis of 49 DIR or DIR-likes genes in rice. The 49 OsDIRs or OsDIR-likes were tandem arranged into ten clusters. The phylogenetic analysis indicated that the 49 rice DIR and DIR-like genes cluster into five distinct subfamilies, DIR-a and four DIRlike subfamilies (DIR-b/d, and DIR-g, DIR-c, DIR-e). Meta-analysis of microarray gene expression datas indicated that all the OsDIRs or OsDIR-likes were expressed almost at the same level but with different patterns: most OsDIRs or OsDIR-likes were expressed exclusively in stigma and ovary and were induced by IAA and BAP; several genes were induced by trans-zeatin (tZ) and DMSO; 23 OsDIRs or OsDIR-likes were responded to abiotic stress. Our analysis also showed that most of these genes could respond to abiotic stresses, which contained cis-regulatory elements. The present study will provide a useful reference for further functional analysis of the DIR genes in rice.

Profiling of skeletal muscle tissue for long non-coding RNAs related to muscle metabolism in the QingYu pig at the growth inflection point

Luo, Jia,Shen, Linyuan,Gan, Mailin,Jiang, Anan,Chen, Lei,Ma, Jideng,Jin, Long,Liu, Yihui,Tang, Guoqing,Jiang, Yanzhi,Li, Mingzhou,Li, Xuewei,Zhang, Shunhua,Zhu, Li Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.8

Objective: Investigation of muscle growth at different developmental stages is an appropriate strategy for studying the mechanisms underlying muscle development and differences in phenotypes. In particular, the muscle development mechanisms and the difference between the fastest and slowest growth rates. Methods: In this study, we used a growth curve model to fit the growth inflection point (IP) of QingYu pigs and compared differences in the long non-coding RNA (lncRNA) transcriptome of muscle both at the growth IP and plateau phase (PP). Results: The growth curve of the QingYu pig had a good fit (R² = 0.974) relative to a typical S-curve and reached the IP at day 177.96. At the PP, marbling, intramuscular fat, and monounsaturated fatty acids had increased significantly and the percentage of lean muscle and polyunsaturated fatty acids had decreased. A total of 1,199 mRNAs and 62 lncRNAs were differentially expressed at the IP compared with the PP. Additional to gene ontology and Kyoto encyclopedia of genes and genomes pathway analyses, these differentially expressed protein coding genes were principally related to muscle growth and lipid metabolism. Conclusion: Our results suggest that the identified differentially expressed lncRNAs, could play roles in muscle growth, fat deposition and regulation of fatty acid composition at the IP and PP.

Whole-genome sequence association study identifies cyclin dependent kinase 8 as a key gene for the number of mummified piglets

Wu Pingxian,Chen Dejuan,Wang Kai,Wang Shujie,Liu Yihui,Jiang Anan,Xiao Weihang,Jiang Yanzhi,Zhu Li,Xu Xu,Qiu Xiaotian,Li Xuewei,Tang Guoqing 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.1

Objective: Pigs, an ideal biomedical model for human diseases, suffer from about 50% early embryonic and fetal death, a major cause of fertility loss worldwide. However, identifying the causal variant remains a huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) and candidate genes for the number of mummified (NM) piglets using the imputed whole-genome sequence (WGS) and validate the potential candidate genes. Methods: The imputed WGS was introduced from genotyping-by-sequencing (GBS) using a multi-breed reference population. We performed genome-wide association studies (GWAS) for NM piglets at birth from a Landrace pig populatiGWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase on. A total of 300 Landrace pigs were genotyped by GBS. The whole-genome variants were imputed, and 4,252,858 SNPs were obtained. Various molecular experiments were conducted to determine how the genes affected NM in pigs. Results: A strong GWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase 8 (CDK8) gene, which plays a crucial role in embryonic retardation and lethality. Based on the molecular experiments, we found that Y-box binding protein 1 (YBX1) was a crucial transcription factor for CDK8, which mediated the effect of CDK8 in the proliferation of porcine ovarian granulosa cells via transforming growth factor beta/small mother against decapentaplegic signaling pathway, and, as a consequence, affected embryo quality, indicating that this pathway may be contributing to mummified fetal in pigs. Conclusion: A powerful imputation-based association study was performed to identify genes associated with NM in pigs. CDK8 was suggested as a functional gene for the proliferation of porcine ovarian granulosa cells, but further studies are required to determine causative mutations and the effect of loci on NM in pigs.

3-D Deformation Behavior Simulation of Cable Stitch Based on Particle System in Weft Knitted Fabrics

Sha Sha,Lei Luo,Zhongmin Deng,Dapeng Yuan,Bin Li,Xuewei Jiang,Hui Tao,Qufu Wei 한국섬유공학회 2018 Fibers and polymers Vol.19 No.9

The static simulation of weft knitting can be efficiently realized by graphics simulation techniques, but there still remains a challenge for mechanical models. The lack of practical mechanical models significantly limit the realistic deformation behaviors of complex cable stitches, which lead to a great different between the simulation effect and the actual fabric. In order to obtain the deformation behavior and volumetric performance of cable stitch, loop models were built based on an improved particle system in this work. Compared with plain weft knitted, the offset value of bonding points of cable stitches were measured. By analyzing the relationship between the deformation of loops and the displacement of the particles, the deformation behavior of cable stitch was simulated. Velocity-Verlet integration was introduced to simulate cable stitches and the stable results were obtained. The results show that these models and algorithm displayed the accurate deformation behavior of cable stitches, as demonstrated by qualitative comparisons to measure the deformations of actual samples.

Evaluation of coat color inheritance and production performance for crossbreed from Chinese indigenous Chenghua pig crossbred with Berkshire

Li Yujing,Yuan Rong,Gong Zhengyin,Zou Qin,Wang Yifei,Tang Guoqing,Zhu Li,Li Xuewei,Jiang Yanzhi 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.10

Objective: This work was to determine coat inheritance and evaluate production performance for crossbred pigs from Berkshire×Chenghua (BC) compared with Chinese indigenous Chenghua (CH) pigs. Methods: The coat color phenotypes were recorded for more than 16,000 pigs, and the genotypes of melanocortin 1 receptor (MCIR) gene were identified by sequencing. The reproductive performance of 927 crossbred BC F4 gilts and 320 purebred CH gilts was recorded. Sixty pigs of each breed were randomly selected at approximately 60 days of age to determine growth performance during fattening period, which lasted for 150 days for BC pigs and 240 days for CH pigs. At the end of the fattening period, 30 pigs of each breed were slaughtered to determine carcass composition and meat quality. Results: The coat color of BC pigs exhibits a “dominant black” hereditary pattern, and all piglets derived from boars or sows genotyped ED1ED1 homozygous for MC1R gene showed a uniform black coat phenotype. The BC F4 gilts displayed a good reproductive performance, showing a higher litter and tear size and were heavier at farrowing litter and at weaning litter than the CH gilts, but they reached puberty later than the CH gilts. BC F4 pigs exhibited improved growth and carcass characteristics with a higher average daily live weight gain, lower feed-to-gain ratio, and higher carcass lean meat rate than CH pigs. Like CH pigs, BC F4 pigs produced superior meat-quality characteristics, showing ideal pH and meat-color values, high intramuscular fat content and water-holding capacity, and acceptable musclefiber parameters. C18:1, C16:0, C18:0, and C18:2 were the main fatty acids in M. longissimus lumborum in the two breeds, and a remarkably high polyunsaturated/saturated fatty acid ratio of ~0.39 was observed in the BC F4 pigs. Conclusion: The BC F4 pigs exhibit a uniform black coat pattern and acceptable total production performance. Objective: This work was to determine coat inheritance and evaluate production performance for crossbred pigs from Berkshire×Chenghua (BC) compared with Chinese indigenous Chenghua (CH) pigs.Methods: The coat color phenotypes were recorded for more than 16,000 pigs, and the genotypes of melanocortin 1 receptor (MCIR) gene were identified by sequencing. The reproductive performance of 927 crossbred BC F4 gilts and 320 purebred CH gilts was recorded. Sixty pigs of each breed were randomly selected at approximately 60 days of age to determine growth performance during fattening period, which lasted for 150 days for BC pigs and 240 days for CH pigs. At the end of the fattening period, 30 pigs of each breed were slaughtered to determine carcass composition and meat quality.Results: The coat color of BC pigs exhibits a “dominant black” hereditary pattern, and all piglets derived from boars or sows genotyped E^D1E^D1 homozygous for MC1R gene showed a uniform black coat phenotype. The BC F4 gilts displayed a good reproductive performance, showing a higher litter and tear size and were heavier at farrowing litter and at weaning litter than the CH gilts, but they reached puberty later than the CH gilts. BC F4 pigs exhibited improved growth and carcass characteristics with a higher average daily live weight gain, lower feed-to-gain ratio, and higher carcass lean meat rate than CH pigs. Like CH pigs, BC F4 pigs produced superior meat-quality characteristics, showing ideal pH and meat-color values, high intramuscular fat content and water-holding capacity, and acceptable musclefiber parameters. C18:1, C16:0, C18:0, and C18:2 were the main fatty acids in M. longissimus lumborum in the two breeds, and a remarkably high polyunsaturated/saturated fatty acid ratio of ~0.39 was observed in the BC F4 pigs.Conclusion: The BC F4 pigs exhibit a uniform black coat pattern and acceptable total production performance.