Prognostic Role of Circulating Tumor Cells in Patients with Pancreatic Cancer: a Meta-analysis

Ma, Xue-Lei,Li, Yan-Yan,Zhang, Jing,Huang, Jing-Wen,Jia, Hong-Yuan,Liu, Lei,Li, Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Isolation and characterization of circulating tumor cells (CTCs) in patients suffering from a variety of different cancers have become hot biomarker topics. In this study, we evaluated the prognostic value of CTCs in pancreatic cancer. Materials and Methods: Initial literature was identified using Medline and EMBASE. The primary data were hazard ratios (HRs) with 95% confidence intervals (CIs) of survival outcomes, including overall survival (OS) and progression free survival/recurrence free survival (PFS/RFS). Results: A total of 9 eligible studies were included in this meta-analysis, published between 2002 and 2013. The estimated pooled HR and 95%CI for OS for all studies was 1.64 (95%CI 1.39-1.94, p<0.00001) and the pooled HR and 95%CI for RFS/DFS was 2.36 (95%CI 1.41-3.96, p<0.00001). The HRs and 95%CIs for OS and RFS/DFS in patients before treatment were 1.93 (95%CI 1.26-2.96, p=0.003) and 1.82 (95%CI 1.22-2.72, p=0.003), respectively. In patients receiving treatment, the HRs and 95%CI for OS and RFS/DFS were 1.37 (95%CI 1.00-1.86, p=0.05) and 1.89 (95%CI 1.01-3.51, p=0.05), respectively. Moreover, the pooled HR and 95%CI for OS in the post-treatment group was 2.20 (95%CI 0.80-6.02, p=0.13) and the pooled HR for RFS/DFS was 8.36 (95%CI 3.22-21.67, p<0.0001). Conclusions: The meta-analysis provided strong evidence supporting the proposition that CTCs detected in peripheral blood have a fine predictive role in pancreatic patients especially on the time point of post-treatment.

Diagnostic Accuracy of 18F-FDG-PET in Patients with Testicular Cancer: a Meta-analysis

Zhao, Jing-Yi,Ma, Xue-Lei,Li, Yan-Yan,Zhang, Bing-Lan,Li, Min-Min,Ma, Xue-Lei,Liu, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Objective: Fluorine-18-fluorodeoxyglucose positron emission tomography (18F-FDG-PET) is a new technique for identifying different malignant tumors using different uptake values between tumor cells and normal tissues. Here we assessed the diagnostic accuracy of 18F-FDG-PET in patients with testicular cancer by pooling data of existing trials in a meta-analysis. Methods: PubMed/MEDLINE, Embase and Cochrane Central Trials databases were searched and studies published in English relating to the diagnostic value of FDG-PET for testicular cancer were collected. The summary receiver operating characteristic (SROC) curve was used to examine the FDG-PET accuracy. Results: A total of 16 studies which included 957 examinations in 807 patients (median age, 31.1 years) were analyzed. A meta-analysis was performed to combine the sensitivity and specificity and their 95% confidence intervals (CIs), from diagnostic odds ratio (DOR), positive likelihood ratios (PLR), negative likelihood ratio (NLR). SROC were derived to demonstrate the diagnostic accuracy of FDG-PET for testicular cancer. The pooled sensitivity and specificity were 0.75 (95% confidence interval (CI), 0.70-0.80) and 0.87 (95% CI, 0.84-0.89), respectively. The pooled DOR was 35.6 (95% CI, 12.9-98.3). The area under the curve (AUC) was 0.88. The pooled PLR and pooled NLR were 7.80 (95% CI, 3.73-16.3) and 0.31 (95% CI, 0.23-0.43), respectively. Conclusion: In patients with testicular cancer, 18F-FDG-PET demonstrated a high SROC area, and could be a potentially useful tool if combined with other imaging methods such as MRI and CT. Nevertheless, the literature focusing on the use of 18F-FDG-PET in this setting still remains limited.

A highly active Z-scheme NiGa2O4/anthraquinone/MoO3 photocatalyst via charge transfer for sunlight photocatalytic simultaneous conversions of nitrite and sulfite

Xue Ma,Siyi Li,Zhihui Qu,Meng Zhang,Jing Qiao,Xin Cui,Chunquan Wang,Jun Wang,Youtao Song 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.78 No.-

The separation efficiency of photogenerated electron-hole pairs (e –h+) is an important factor for ahighly photocatalytic activity of Z-scheme photocatalytic system. In order to suppress the recombining ofphotogenerated e and h+ and enhance the electron transfer rate, anthraquinone (AQ) as special electrontransfer channel is combined with NiGa2O4 and MoO3, forming a Z-scheme NiGa2O4/AQ/MoO3photocatalyst. Due to unique chemical and physical properties, AQ provides a novel electron transmissionway. Compared with traditional conductive channels (for example, noble metals and graphene), in thisway, the AQ may greatly enhance the electron transfer efficiency. In addition, the related morphology,optical properties and chemical composition were investigated by a sequence of characterizationmethods. The influences of photocatalyst species, irradiation time and used times in simultaneouslyconverting nitrite and sulfite under simulated sunlight were also studied. The results indicate that theprepared Z-scheme NiGa2O4/AQ/MoO3 photocatalyst exhibits a much high activity. The conversion ratesof nitrite and sulfite can reach 89.81% and 94.47%, respectively, under simulated sunlight irradiation forfour hours. The enhanced photocatalytic activity is attributed to the fact that AQ can rapidly transferelectron by inner charge transfer. This charge transfer way can provide a new idea to design subsequentphotocatalysts.

Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis

Xue Song,Jing Guo,Wen-xiu Ma,Zhi-yuan Ji,Li-fang Zou,Gong-you Chen,Hua-song Zou 한국미생물학회 2015 The journal of microbiology Vol.53 No.5

To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis revealed that each of the mutants carried a single copy of Tn5. The flanking sequence was identified by plasmid rescue and 18 different ORFs were identified in the genome sequence. Of these 18 ORFs, seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis. Real-time PCR analysis showed that the seven genes were upregulated when the bacteria were grown in citrus plants, suggesting that the expression of these genes was essential for canker development.

Patterns of Failure and Survival Trends in 3,808 Patients with Stage II Nasopharyngeal Carcinoma Diagnosed from 1990 to 2012: A Large-Scale Retrospective Cohort Study

Xue-Song Sun,Di-Han Liu,Sai-Lan Liu,Qiu-Yan Chen,Shan-Shan Guo,Yue-Feng Wen,Li-Ting Liu,Hao-Jun Xie,Qing-Nan Tang,Yu-Jing Liang,Xiao-Yun Li,Jin-Jie Yan,Ming-Huang Hong,Jun Ma,Lin-Quan Tang,Hai-Qiang M 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose The purpose of this study was to investigate the survival trends and patterns of failure in patients with stage II nasopharyngeal carcinoma (NPC) treated with radiotherapy (RT) and chemotherapy over the last 20 years. Materials and Methods Thirty-eight hundred and eight patients diagnosed with stage II NPC between January 1990 and December 2012 were involved in this retrospective cohort study. All patients were treated with RT. According to the main imaging techniques and RT technology, we categorized these patients into four calendar periods: 1990-1996, 1997-2002, 2003-2007, and 2008-2012. Overall survival (OS), progression-free survival (PFS), locoregional relapse-free survival (LRFS), and distant metastasis–free survival (DMFS) were served as the clinical outcome. Results After a median follow-up period of 84.7 months, we observed increasing trends in survival and disease control. The 3- and 5-year OS rates increased from 87.1% and 78.7% in the first calendar period to 97.4% and 94.5% in the last calendar period, respectively (p < 0.001). Additionally, significant increasing trends could be seen in the PFS and LRFS during the four calendar periods. In the subgroup analysis, the LRFS in patients older than 50 years at diagnosis showed greater improvement than younger patients. However, the rate of distant metastasis was stable and relatively low, as the 5-year DMFS ranged from 90.5% to 94.7% among the four calendar periods. Conclusion The survival rates in patients with stage II NPC showed increasing trends from 1990 to 2012. The advance of RT provided excellent locoregional control and enhanced OS.

Numerical analysis and horizontal bearing capacity of steel reinforced recycled concrete columns

Hui Ma,Jian-yang Xue,Yunhe Liu,Jing Dong 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.22 No.4

This paper simulates the hysteretic behavior of steel reinforced recycled concrete (SRRC) columns under cyclic loads using OpenSees software. The effective fiber model and displacement-based beam-column element in OpenSees is applied to each SRRC columns. The Concrete01 material model for recycled aggregate concrete (RAC) and Steel02 material model is proposed to perform the numerical simulation of columns. The constitutive models of RAC, profile steel and rebars in columns were assigned to each fiber element. Based on the modelling method, the analytical models of SRRC columns are established. It shows that the calculated hysteresis loops of most SRRC columns agree well with the test curves. In addition, the parameter studies (i.e., strength grade of RAC, stirrups strength, steel strength and steel ratio) on seismic performance of SRRC columns were also investigated in detail by OpenSees. The calculation results of parameter analysis show that SRRC columns suffered from flexural failure has good seismic performance through the reasonable design. The ductility and bearing capacity of columns increases as the increasing magnitude of steel strength, steel ratio and stirrups strength. Although the bearing capacity of columns increases as the strength grade of RAC increases, the ductility and energy dissipation capacity decreases gradually. Based on the test and numerical results, the flexural failure mechanism of SRRC columns were analysed in detail. The computing theories of the normal section of bearing capacity for the eccentrically loaded columns were adopted to calculate the nominal bending strength of SRRC columns subjected to vertical axial force under lateral cyclic loads. The calculation formulas of horizontal bearing capacity for SRRC columns were proposed based on their nominal bending strength.

Facile Synthesis of Thermo-responsive TiO2/PNIPAM Composite with Switchable Photocatalytic Performance

Yinli Duan,Jing Ma,Jiao Liu,Liang-Sheng Qiang,Juanqin Xue 한국섬유공학회 2020 Fibers and polymers Vol.21 No.4

In this paper, a facile and efficient method was performed to prepare the thermo-responsive Poly(Nisopropylacrylamide)/TiO2 (TiO2/PNIPAM) with the dispersing of TiO2 nanoparticles into PNIPAM. The structures ofnanoparticles and the grafted polymer were confirmed by X-ray diffraction (XRD), Raman spectra, Fourier transforminfrared spectra (FT-IR) and Dynamic Light Scattering (DLS). The interactions between the two components were studied byX-ray photoelectron spectroscopy (XPS), and the morphology of TiO2/PNIPAM was observed by scanning electronmicroscopy (SEM). The results showed that TiO2 exhibited homogeneous dispersion, without sacrificing the properties ofTiO2. Furthermore, The composite was used to photocatalytic degradation of Methylene blue (MB). The highest MB removalefficiency is 86.3 %. The photocatalytic activities above or below the lower critical solution temperature (LCST) of PNIPAMwas also investigated. The experiment results show that the existence of PNIPAM contributed not only the thermo-responsiveabilities to TiO2, but also the enhanced photocatalytic activities to TiO2. In addition, the corresponding mechanism ofenhanced photocatalytic activity was proposed. The results manifest that the well-designed TiO2/PNIPAM is a promisingcandidate in wastewater treatment application.

Associations of Single Nucleotide Polymorphisms in miR-146a, miR-196a, miR-149 and miR-499 with Colorectal Cancer Susceptibility

Du, Wei,Ma, Xue-Lei,Zhao, Chong,Liu, Tao,Du, Yu-Liang,Kong, Wei-Qi,Wei, Ben-Ling,Yu, Jia-Yun,Li, Yan-Yan,Huang, Jing-Wen,Li, Zi-Kang,Liu, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Background: MicroRNAs (miRNAs) are an abundant class of endogenous small non-coding RNAs of 20-25 nucleotides in length that function as negative gene regulators. MiRNAs play roles in most biological processes, as well as diverse human diseases including cancer. Recently, many studies investigated the association between SNPs in miR-146a rs2910164, miR-196a2 rs11614913, miR-149 rs229283, miR-499 rs3746444 and colorectal cancer (CRC), which results have been inconclusive. Methodology/Principal Findings: PubMed, EMBASE, CNKI databases were searched with the last search updated on November 5, 2013. For miR-196a2 rs11614913, a significantly decreased risk of CRC development was observed under three genetic models (dominant model: OR = 0.848, 95%CI: 0.735-0.979, P = 0.025; recessive model: OR = 0.838, 95%CI: 0.721-0.974, P = 0.021; homozygous model: OR = 0.754, 95%CI: 0.627-0.907, P = 0.003). In the subgroup analyses, miR-$196a2^*T$ variant was associated with a significantly decreased susceptibility of CRC (allele model: OR = 0.839, 95%CI: 0.749-0.940, P = 0.000; dominant model: OR = 0.770, 95%CI: 0.653-0.980, P = 0.002; recessive model: OR = 0.802, 95%CI: 0.685-0.939, P = 0.006; homozygous model: OR = 0.695, 95%CI: 0.570-0.847, P = 0.000). As for miR-149 rs2292832, the two genetic models (recessive model: OR = 1.199, 95% CI 1.028-1.398, P = 0.021; heterozygous model: OR = 1.226, 95% CI 1.039-1.447, P = 0.013) demonstrated increased susceptibility to CRC. On subgroup analysis, significantly increased susceptibility of CRC was found in the genetic models (recessive model: OR = 1.180, 95% CI 1.008-1.382, P = 0.040; heterozygous model: OR = 1.202, 95% CI 1.013-1.425, P = 0.013) in the Asian group. Conclusions: These findings supported that the miR-196a2 rs11614913 and miR-149 rs2292832 polymorphisms may contribute to susceptibility to CRC.

Preparation of ortho-symmetric double (OSD) Z-scheme SnO2\CdSe/ Bi2O3 sonocatalyst by ultrasonic-assisted isoelectric point method for effective degradation of organic pollutants

Siyi Li,Meng Zhang,Xue Ma,Jing Qiao,Hongbo Zhang,Jun Wang,Youtao Song 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.72 No.-

In this work, an ortho-symmetric double (OSD) Z-scheme SnO2\CdSe/Bi2O3 sonocatalyst is skillfullydesigned and synthesized via ultrasonic-assisted isoelectric point method and then the sonocatalyticactivity is studied under ultrasonic irradiation in the degradation of organic contaminants. The effects ofultrasonic irradiation time, sonocatalyst dosage, initial Rhodamine B concentration, organic dye kind andused times are investigated systemically. The formation of OH and h+ are confirmed by using t-BuOH andoxalate, respectively. The Rhodamine B can completely be degraded under given experimental conditionssuch as 10 mg/L Rhodamine B solution, 1.00 g/L OSD Z-scheme SnO2\CdSe/Bi2O3 dosage and 150 minultrasonic process. The formation of mainly intermediate products is studied by Liquid Chromatography–Mass Spectrometry. Moreover, the sonocatalytic reaction mechanism caused by OSD Z-schemeSnO2\CdSe/Bi2O3 is presented.

Layer-by-layer Self-assembly Polyurethane/TiO2 NPs Hybrid Film and Its Photocatalytic Efficiency for Decolorization of Methyl Blue

Yijiong Liu,Juanqin Xue,Yinli Duan,Liang-Sheng Qiang,Jing Ma 한국섬유공학회 2019 Fibers and polymers Vol.20 No.9

In this study, Layer-by-layer (LbL) self-assembly technique was used to fabricate TiO2 films onto glass slides. Polyurethane (PU) was used as a substrate for the immobilization of TiO2, to increase the adsorption ability of thephotocatalyst. The LBL sequence was (PU/TiO2 NPs)n (n is the number of bilayers). These LbL hybrid films werecharacterized by Raman spectra, which confirmed the formation of TiO2 nanoparticles (TiO2 NPs) in the anatase phase withaverage size around 30 nm. The thickness of the film estimated around 1.1±0.2 μm as determined by scanning electronmicroscopy (SEM). UV-vis spectra confirmed the linear and regular growth of the films with the number of bilayers. Thephotocatalytic property of the films was investigated by the decomposition of methyl blue (MB) under UV-light irradiation. Furthermore, the reusability studies revealed that the fabricated film could be repeatedly used for up to six times withoutaffecting the photocatalytic activity. In addition, the corresponding mechanism of enhanced photocatalytic activity wasproposed on the basis of the investigated results from reactive species scavenging of (PU/TiO2 NPs)10. The active species h+played the most important role in the photocatalytic reaction. This work manifested that the LbL films can be handled moreeasily than TiO2 NPs in suspension form. Therefore, as-synthesized film is a promising candidate in wastewater treatmentapplication.