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- 저자 : Wen-xiu Ma (검색결과 5 건)
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Design and Implementation on Spatial Science and Technology Information Database of CSI
Chen, Xiu Wan,Deng, Zheng Quan,Lu, Zhi Gao,Ma, Jia,Lin, Jia Yuan,Zhang, Wen Jiang,Luo, Tianfu,Liu, Baofu 대한원격탐사학회 2000 International Symposium on Remote Sensing Vol.16 No.1
Remote Sensing technology, which is characterized by producing imagery an multi-platform, different temporal and spatial resolution, has greatly improved mankind's capability of acquisition, processing and application of spatial information. The increase of spatial data sources and the development, applications and industrialization of spatial information technology are urging the need of spatial data sharing and exchanging. Based an a brief introduction an the China Spatial Information Network (CSI) and its database system, the CSI Spatial Science and Technology Information Database (SSTID) management system was designed and implemented in this paper.
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A novel M2e-multiple antigenic peptide providing heterologous protection in mice
Feng Wen,Ji-Hong Ma,Hai Yu,Fu-Ru Yang,Meng Huang,Yan-Jun Zhou,Ze-Jun Li,Xiu-Hui Wang,Guo-Xin Li,Yi-Feng Jiang,Wu Tong,Guangzhi Tong 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
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Wettability contrast on the surface of CuO nanostructures for highly efficient fog harvesting
He Wen-xin,Jin Lian-lian,Ma Xiu-jia,Li Xiao,Li Jia,Wang Xue-wei 한국물리학회 2023 Current Applied Physics Vol.53 No.-
Fog collection can provide a sustainable and efficient method for solving the shortage of water resources. In this article, CuO nanostructures were designed and synthesized on the Cu mesh by a one-step oxidation method and used to collect water from fog. Their morphology and surface wettability are characterized through scanning electron microscopy and dynamic contact angle measuring device, while the process of fog collection is investigated by a high-speed camera. The fog collection efficiency depends on the microstructure of the sample surface, which could be well controlled by adjusting the reaction temperature. Compared with CuO nanoneedles and CuO nanowire clusters, CuO nanosheets promote the rapid falling of liquid droplets, improving the fog harvesting efficiency, which can achieve 3168 mg/cm2 within 120 s. CuO nanosheets have ultra-low adhesion due to high roughness and high air restraint ability, accelerating the transport of water droplets and reducing the re-evaporation of water droplets.
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Xue Song,Jing Guo,Wen-xiu Ma,Zhi-yuan Ji,Li-fang Zou,Gong-you Chen,Hua-song Zou 한국미생물학회 2015 The journal of microbiology Vol.53 No.5
To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis revealed that each of the mutants carried a single copy of Tn5. The flanking sequence was identified by plasmid rescue and 18 different ORFs were identified in the genome sequence. Of these 18 ORFs, seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis. Real-time PCR analysis showed that the seven genes were upregulated when the bacteria were grown in citrus plants, suggesting that the expression of these genes was essential for canker development.
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Integrin-linked Kinase Functions as a Tumor Promoter in Bladder Transitional Cell Carcinoma
Wang, De-Lin,Lan, Jian-Hua,Chen, Liang,Huang, Biao,Li, Zeng,Zhao, Xiu-Min,Ma, Qiang,Sheng, Xia,Li, Wen-Bin,Tang, Wei-Xue Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6
The aim of this study was to elucidate the role of the integrin-linked kinase (ILK) gene in development of human bladder transitional cell carcinoma (BTCC). Expression of ILK protein and ILK mRNA in 56 cases of human BTCC tissue and in 30 cases of adjacent normal bladder tissue was detected by immunohistochemistry S-P and reverse transcription polymerase chain reaction (RT-PCR), respectively. Four specific miRNA RNAi vectors targeting human ILK were synthesized and transfected into BIU-87 cells by liposome to obtain stable expression cell strains. The influence of ILK on proliferation of BTCC was detected by MTT, FCM on athymic mouse tumorigenesis. The positive rate of ILK protein in BTCC tissue (53.6%) was much higher than adjacent normal bladder tissue (10.0%) (p<0.05). Similarly, expression of ILK mRNA in BTCC tissue ($0.540{\pm}0.083$) was significantly higher than in adjacent normal bladder tissue ($0.492{\pm}0.070$) (p<0.05). MTT showed that the proliferation ability of miRNA-ILK transfected group was clearly decreased (p<0.05), the cell cycle being arrested in G0/G1-S, an tumorigenesis in vivo was also significantly reduced (p<0.05). ILK gene transcription and protein expression may be involved in the development of BTCC, so that ILK might be the new marker for early diagnosis and the new target for gene treatment.
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