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Dong Dong Zhang,Yang Fang Wu,Wei Xia Chen,Yao Xu,Si Yan Liu,Huang Huang Luo,Guang Mei Jiang,Yue Wu,Peng Hu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Renal osteodystrophy (ROD) occurs as early as chronic kidney disease (CKD) stage 2 and seems ubiquitous in almost all pediatric patients with CKD stage 5. Fibroblast growth factor (FGF)-23, a bone-derived endocrine regulator of phosphate homeostasis, is overexpressed in CKD and disturbs osteoblast differentiation and matrix mineralization. In contrast, C-type natriuretic peptide (CNP) acts as a potent positive regulator of bone growth. In the present study, we infused CNP into uremic rats and observed whether CNP could attenuate ROD through the inhibition of FGF-23 cascades. In uremic rats, CNP administration significantly alleviated renal dysfunction, calcium phosphate metabolic disorders, hypovitaminosis D, secondary hyperparathyroidism, the decrease in bone turnover markers and retarded bone pathological progression. More importantly, within FGF-23/mitogen-activated protein kinase (MAPK) signaling, the fibroblast growth factor receptor-1, Klotho and alternative (STAT-1/phospho-STAT-1) elements were upregulated by CNP, whereas FGF-23, RAF-1/phospho-RAF-1, and downstream (ERK/phospho-ERK and P38/phospho-P38) elements were paradoxically underexpressed in bone tissue. Therefore, CNP exerts a therapeutic effect on ROD through inhibition of FGF-23/MAPK signaling at the RAF-1 level.
Xu-Dong TANG,Nan LIU,Wei Wang,Jieyun Yan,Li Xu,Zhongyuan Shen 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1
The silkworm (Bombyx mori) is an important insect for various industrial applications and is also a model insect for the order Lepidoptera. The silkworm is more resistant to deltamethrin at high temperatures than at low temperatures. To elucidate the role of the negative temperature coefficient of deltamethrin in the silkworm, digital gene expression (DGE) analysis was performed for the identification of differentially expressed genes in silkworms challenged with deltamethrin at different temperatures. Compared with deltamethrin exposure at 30 °C, silkworms treated at 20 °C exhibited more severe stress responses, and gene sets associated with peptidoglycan recognition protein (PGRP),mucin, and antimicrobial peptides (AMPs)were downregulated dramatically. Similarly, a decrease in genes related to reactive oxygen species scavenging was also detected, which may have resulted in an imbalance between radical-generating and radical-scavenging systems. These results indicated that the lower mortality rate after deltamethrin treatment at high temperature was caused by a series of gene alterations (quantitative increases) rather than qualitative changes.
Cr(VI) Resistance and Removal by Indigenous Bacteria Isolated from Chromium-Contaminated Soil
( Dong Yan Long ),( Xian Jin Tang ),( Kuan Cai ),( Guang Cun Chen ),( Chao Feng Shen ),( Ji Yan Shi ),( Ling Gui Chen ),( Ying Xu Chen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing ability, with 100 mg/l Cr(VI) being completely removed within 144 h. It could effectively remove Cr(VI) over a wide pH range from 5.5 to 9.5, with the optimal pH of 8.5. The amount of Cr(VI) removed increased with initial Cr(VI) concentration. Data from the time-course analysis of Cr(VI) removal by strain LY6 followed first-order kinetics. Based on the 16S rRNA gene sequence, strain LY6 was identified as Pseudochrobactrum asaccharolyticum, a species that had never been reported for Cr(VI) removal before. Transmission electron microscopy and energy dispersive X-ray spectroscopy analysis further confirmed that strain LY6 could accumulate chromium within the cell while conducting Cr(VI) removal. The results suggested that the indigenous bacterial strain LY6 would be a new candidate for potential application in Cr(VI) pollution bioremediation.
Effect of Portal Vein Chemotherapy on Liver Metastasis after Surgical Resection of Colorectal Cancer
Yu, Dong-Sheng,Li, Ying,Huang, Xin-En,Lu, Yan-Yan,Wu, Xue-Yan,Liu, Jin,Cao, Jie,Xu, Xia,Xiang, Jin,Wang, Guo-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9
Objective: To explore the effect of portal vein chemotherapy on liver metastasis after surgical resection of colorectal cancer. Methods: Patients fulfilling the eligibility criteria were assigned to receive either surgery plus 1-week continuous infusion of 5-FU (study group) or surgery alone (observational group). Patients in the study group received portal vein chemotherapy, whereby 5-FU (1000 mg/d) and heparin (5000 IU/d) infusion was initiated from the day of surgery and lasted for 7 consecutive days. Liver metastasis was monitored during five years follow-up postoperatively. Results: Sixty four patients were recruited and assigned to the study group (12 with colon and 20 with rectal cancer) or the control group (10 with colon and 22 with rectal cancer). Liver metastasis rate was 12.5% in study and 25.0% in observational group, the difference being significant (P<0.01). Conclusion: Portal vein chemotherapy could be an effective treatment in preventing liver metastasis after surgical resection of colorectal cancer.
Ifosfamide-containing Regimens for Treating Patients with Osteosarcomas
Li, Yan-Yan,Jiang, Xiao-Ming,Dong, Yi-Guo,Xu, Gang,Ma, Yu-Bo Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22
Background: This systemic analysis was conducted to evaluate the efficacy and safety of an ifosfamide-containing regimen in treating patients with osteosarcoma. Methods: Clinical studies evaluating the efficacy and safety of Ifosfamide-containing regimen on response and safety for patients with osteosarcoma were identified by using a predefined search strategy. Pooled response rate (RR) of treatment were calculated. Results: When ifosfamide-containing regimens were evaluated, 4 clinical studies which including 134 patients with osteosarcoma were considered eligible for inclusion. Systemic analysis suggested that, in all patients, pooled RR was 44.8% (60/134) in ifosfamide-containing regimens. Major adverse effects were neutropenia, leukopenia, and fatigue inIfosfamide-containing regimens; No treatment related death occurred in cantharidin combined regimens. Conclusion: This systemic analysis suggests that ifosfamide-containing regimens are associated with good response rate and acceptable toxicity in treating patients with osteosarcoma, but this result should be confirmed by randomized clinical trials.
Preparation of porous cordierite ceramic with acid-leached coal gangue
Wang Xu-Dong,Xu Hai-Yan,Zhang Feng-Jun,Li Dong-Cai,Wang Ai-Guo,Sun Dao-Sheng,오원춘 한국세라믹학회 2020 한국세라믹학회지 Vol.57 No.4
The eff ect of impurities in coal gangue on the sintering and properties (porosity and compressive strength) of porous cordierite ceramics has been investigated by adjusting the amount of impurities by controlling the acid–gangue ratio during acid leaching. The sintering behavior and microstructure of the porous ceramics have been characterized via X-ray diff ractometry and scanning electron microscopy. The eff ects of both the acid–gangue ratio and sintering temperature on the porosity and compressive strength have been also investigated. The results indicated that both porosity and compressive strength of the porous cordierite ceramics prepared using acid-leached coal gangue were evidently improved. The content of the impurities in coal gangue was adjusted via acid leaching by controlling the acid–gangue ratio. The appropriate content of impurities in coal gangue facilitated the sintering process and improved the porosity and compressive strength of the obtained porous cordierite ceramics.
Culture of a Whole Porcine Liver Ex Situ without Red Blood Cells
( Jing Dong ),( Lingling Xia ),( Hefang Shen ),( Congwen Bian ),( Sujin Bao ),( Ming Zhang ),( Yan Dai ),( Yanhong Xu ),( Qiru Xiong ),( Jianjian Xu ),( Lili Xu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Liver transplantation is an effective approach to end-stage liver disease. Shortage of donor liver and increased waiting time for liver transplantation necessitate the development of an organ culture system by which livers can be cultured and maintained ex situ for a prolonged period of time. The aim of this work is to test whether cell culture condition in vitro could be used to culture whole livers ex situ without the use of erythrocytes. Methods: Eight castrated male land race/farm young porcine livers were exposed to 30 min warm ischemia and 30 min cold perfusion. Livers were isolated and connected to an ex situ liver culture system using a standard culture medium RPMI 1640 supplied with 10% of fetal calf serum and sufficient dissolved oxygen under a normothermic condition for 6 hours. Metabolic biomarkers, bile and urea production, hepatic cell viability, and histology analysis of biopsies were performed and analyzed. Results: Dissociated porcine hepatic cells survived and grew in vitro under the standard RPMI 1640 culture medium. When the same RPMI 1640 medium supplemented with 10% of FCS and sufficient oxygen was used to culture livers ex situ, over 98% of liver cells were viable for at least 6 hours during ex situ whole organ culture based on the results from biochemical assays. Conclusions: Our data demonstrate that the liver culture system established in this work can be used to culture whole livers ex situ in the absence of erythrocytes.
Xu Bilin,Li Zhiliang,Liu Yan,Zhang Wanjing,Yu Jiaojun,Dong Hongjin,Zhang Jialiang,Wang Shuzhen 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2
Rhododendron molle G. Don, an endemic species of the Ericaceae family, possesses valuable medicinal and horticultural values. In this research, R. molle cp genome proved to be a typical quadripartite structure with the length of 200,878 bp. In particular, the lengths of large single-copy region (LSC), small single-copy region (SSC), and inverted repeat regions (IR) were 198,019 bp, 629 bp, and 1117 bp, respectively. Among the 149 unique genes, 97 were protein-coding genes, 44 were tRNA genes, and 8 were rRNA genes, respectively. Leucine was the most representative amino acid (10.663%), while Cysteine was the lowest representative (1.178%). A set of 30 codons showed obvious codon usage bias, while 29 were A/U-ending codons. Six gene regions showed high levels of nucleotide diversity (Pi > 0.02). Totally, 273 SSRs were identified. Maximum-likelihood (ML) phylogenetic analysis revealed that R. molle was relatively closed to the R. pulchrum and R. delavayi. High similarity was detected among Ericaceae species, and the coding regions were more conserved than the non-coding regions. Expansion and contraction detected in IR region could be the main length variation in R. molle and related Ericaceae species. This research will supply rich genetic resource for R. molle and related species of Ericaceae.
Three New 11,20-Epoxy-ent-kauranoids from Isodon rubescens
Xu Liu,Ji Zhou Wu,Rui Zhan,Wei Guang Wang,Xue Du,Yan Li,Peng Zhang,Jian Xin Pu,Han Dong Sun 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12
Three rare and new 11,20-epoxy-ent-kaurane diterpenoids, named jianshirubesins D-F (1-3), along with one known analogue (4), were isolated from the aerial parts of Isodon rubescens. Their structures were established by analysis of spectroscopic data. Found in the MTT assay to evaluate the cytotoxicity of compounds 1, 2, and 4, only 1 could selectively inhibit certain cell lines from proliferating. In addition, a simple structure-activity relationship discussion might suggest a new bioactive moiety, different from the α,β-unsaturated ketone group.