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- 저자 : Xiuqing Hu (검색결과 4 건)
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Jianli Hu,Xiaobing Li,Dikang Zhang,Jun Wang,Xiuqing Hu,Yuanqiang Cai 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.9
Vacuum-electroosmosis is a common method of foundation treatment; however, it is disadvantaged by fine soil particles clogging the drainage plate. To overcome this issue, this study treated the dredged fill from vacuum-electroosmosis with different additives. The parameters of discharged water, current, water content, and shear strength of soils treated with different amounts of Ca(OH)2, FeCl3, and NaCl were analyzed. The results showed that different additives have different effects on the vacuum-electroosmosis method for reinforcing dredger filling. Excessive additive contents were found to have an adverse effect on vacuum-electroosmosis, and hence, optimum amounts are required for the three additives. On comparing the optimum dosage of these additives, FeCl3 was observed to be the most energy-saving. From the aspect of anode corrosion and the costs involved, Ca(OH)2 was the most economical. For practical engineering applications, Ca(OH)2 was the most preferable additive. The results of this study provide guidance and scientific criteria for similar dredging foundation treatments.
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Induction of Rhesus Keratinocytes into Functional Ameloblasts by Mouse Embryonic Dental Mesenchyme
Ningsheng Ruan,Chensheng Lin,Xiuqing Dong,Xuefeng Hu,Yanding Zhang 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.2
Fast progresses in stem cell-based tooth tissue engineering have been achieved in recent years in several animal models including the mouse, rat, dog, and pig. Moreover, various postnatal mesenchymal stem cells of dental origin have been isolated and shown capable of differentiating into odontoblasts and generating dentin. Meanwhile, human ker- atinocyte stem/progenitor cells, gingival epithelial cells, and even iPSC-derived epithelium have been demonstrated to be able to differentiate into functional ameloblasts. Translational medicine studies in the nonhuman primate are irreplaceable steps towards clinical application of stem cell-based tissue engineering therapy. In the present study, we first examined the epithelial stem cell markers in the rhesus skin using immunostaining. Keratinocyte stem cells were then isolated from rhesus epidermis, cultured in vitro, and characterized by epithelial stem cell markers. Epithelial sheets of these cultured keratinocytes, which were recombined with E13.5 mouse dental mesenchyme that possesses odontogenic potential in the presence of exogenous FGF8, were induced to differentiate into enamel-secreting ameloblasts. Our results demonstrate that in the presence of appropriate odontogenic signals, rhesus keratinocytes can be induced to gain odontogenic competence and are capable of participating in odontogenesis, indicating that rhesus keratinocytes are an ideal epithelial cell source for further translational medicine study of tooth tissue engineering in nonhuman primates.
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Influence of Dynamic Loading Activation Time on Electro-osmotic Consolidation of Soft Soil
Xiaobing Li,Jianyu Shu,Jun Wang,Hongtao Fu,Peng Wang,Xiuqing Hu 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.11
Electro-osmosis is a suitable method for the consolidation of soft soil. However, the significant potential loss caused by the cracking of the soil near the electrode in the later stages of electro-osmosis hinders further improvement of its efficiency. Dynamic loading is applied to overcome this problem. The dynamic loading activation time is considered to strongly influence the performance of the combined electro-osmosis-dynamic loading process. In this paper, the maximum current ratio (MCR) is proposed as an index of the dynamic loading activation time. Laboratory tests were performed with different dynamic loading activation times to confirm the influence of the dynamic loading time on electro-osmosis consolidation. During the combined processes of dynamic loading and electro-osmosis, the current, electric potential, discharged water, and surface settlement were monitored. In the following testing, the water content and undrained shear strength were measured. Compared with the electro-osmosis method alone, electro-osmosis and dynamic loading was found to yield superior performance improvement. The method described in this paper to determine the optimal dynamic loading activation time as a function of the MCR, and electro-osmosis, exhibits potential as an improved approach for the consolidation of soft soil in engineering practice.
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Biodegrading of Pyrene by a Newly Isolated Pseudomonas putida PL2
Lifeng Ping,Chunrong Zhang,Yahong Zhu,Min Wu,Xiuqing Hu,Zhen Li,Hua Zhao 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5
Polycyclic aromatic hydrocarbons (PAHs) are a class of persistent organic compounds derived from natural sources and anthropogenic processes, which have been recommended as priority pollutants. Degradation of PAHs in the environment is becoming more necessary and urgent. In the current study, strain PL2, which is capable of growing aerobically on pyrene (PYR) as the sole carbon source, was isolated from hydrocarbons-contaminated soil and then identified as Pseudomonas putida by morphological and physiological characteristics as well as 16S rDNA sequence. The strain PL2 was able to degrade 50.0%of the pyrene at 28°C within 6 days in the presence of 50mg/L pyrene, while the strain PL2 degraded 50.0% of the pyrene within 2 days when a solution of 50 mg/L pyrene and 50 mg/L phenanthrene was used. In addition, phenanthrene was shown to increase the biodegradation efficiency of pyrene by the strain PL2. The order of degradation by the strain PL2 was pH 6.0 > pH 7.0 > pH 5.0 > pH 8.0. The degradation rate of PYR in the soil by the strain PL2reached 70.0% at the 10^th day. The dynamics of PYR degradation in soil by PL2 was fit to the first order model and the strain PL2 was shown to efficiently degrade PYR in soil. The current study showed that P. putida PL2 was a novel bacterium that could degrade pyrene and holds great promise for use in PAHs bioremediation in soil.
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