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Juan Zhao,Wei Ma,Weizhi Chen,Jie Gao,Chunling Li,Yahong Tong,Qin Zhou,Xiuling Zhao,Menghua Wang,Huan Xiao,Yanrong Jin 한국통합생물학회 2019 Animal cells and systems Vol.23 No.6
Endometriosis (EMs) is one of the most common gynaecological diseases in women of childbearing age. Astrocyte elevated gene-1 (AEG-1) is associated with the invasion, migration, apoptosis and prognosis of various cancers. However, the roles of AEG-1 in EMs and its corresponding molecular mechanism are still unknown. In this study, animal models of EMs were established and mice were divided into two groups (n = 10): Sham group and EMs group. The EMs cells were isolated from EMs model. The AEG-1 gene was knocked down by shRNA, while the SOCS1 gene was knocked down by siRNA. Histological changes, AEG-1 expression in tissues and inflammatory factors level were detected by H&E staining, immunohistochemistry and ELISA, respectively. RTqPCR and western blotting were used to determine the expression level of related proteins. The present study found AEG-1 was up-regulated in the EMs model. Enhanced AEG-1 promoted inflammatory cell infiltration, and elevated the levels of IL-1β, IL-6, and TNF-α in EM group (p < 0.05). Besides, AEG-1 overexpression promoted the expression of NALP3, ASC and Cleavedcaspase- 1, while decreased SOCS1 level (p < 0.05). Decrease of SOCS1 further promoted the formation of NALP3 inflammasome. The inhibitory effect of AEG-1 on SOCS1 was weakened after the addition of MG-132 (p < 0.01). Furthermore, silencing AEG-1 alone increased SOCS1 level, decreased the levels of inflammatory cytokines, thereby inhibited the formation of NALP3 inflammasome. All these results demonstrated that AEG-1 aggravated inflammation via promoting NALP3 inflammasome formation in murine endometriosis lesions.
Fengnan Lian,Dan Wang,Shuo Yao,Lirui Ge,Yue Wang,Yuyi Zhao,Jinbin Zhao,Xiuling Song,Chao Zhao,Jinhua Li,Yajuan Liu,Minghua Jin,Kun Xu 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.8
This research aimed to detect Escherichia coli O157:H7 in milk based on immunomagnetic probe separation technology and quenching effect of gold nanoparticles to Rhodamine B. Streptavidin-modified magnetic beads (MBs) were combined with biotin-modified antibodies to capture E. coli O157:H7 specifically. Gold nanoparticle (AuNPs) was incubated with sulfhydryl-modified aptamers (SH-Aptamers) to obtain the Aptamers-AuNPs probe. After magnetic beads captured target bacteria and formed a sandwich structure with the gold nanoprobe, Rhodamine B was added into complex to obtain fluorescent signal changes. Our results demonstrated that the established method could detect E. coli O157:H7 in the range of 101–107 CFU/mL, and the limit of detection (LOD) was 0.35 CFU/mL in TBST buffer (pH = 7.4). In milk simulation samples, the LOD of this method was 1.03 CFU/mL. Our research provides a promising approach on the detection of E. coli O157:H7.
Xiuling Bai,Mingchuan Zhang,Qingtao Wu,Ruijuan Zheng,Haixia Zhao,Wangyang Wei 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.6
Due to various reasons, there are generally missing data in datasets. Usually the missing data in these incomplete datasets need to be filled. In this paper, the drawbacks of some existing data filling approaches for incomplete information systems are analyzed based on Rough Set theory. Several similarity relation models are discussed and the Valued Limited Tolerance Relation model is proposed. A data filling algorithm based on the Valued Limited Tolerance Relation model is put forward. This approach makes full use of the similarity of objects and selects the object which is the most similar to the incomplete object. More missing data can be filled scientifically. The experimental results show that this approach is effective.
A real-time sorting algorithm for in-beam PET of heavy-ion cancer therapy device
Ke, Lingyun,Yan, Junwei,Chen, Jinda,Wang, Changxin,Zhang, Xiuling,Du, Chengming,Hu, Minchi,Yang, Zuoqiao,Xu, Jiapeng,Qian, Yi,She, Qianshun,Yang, Haibo,Zhao, Hongyun,Pu, Tianlei,Pei, Changxu,Su, Hong Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.10
A real-time digital time-stamp sorting algorithm used in the In-Beam positron emission tomography (In-Beam PET) is presented. The algorithm is operated in the field programmable gate array (FPGA) and a small amount of registers, MUX and memory cells are used. It is developed for sorting the data of annihilation event from front-end circuits, so as to identify the coincidence events efficiently in a large amount of data. In the In-Beam PET, each annihilation event is detected by the detector array and digitized by the analog to digital converter (ADC) in Data Acquisition Unit (DAQU), with a resolution of 14 bits and sampling rate of 50 MS/s. Test and preliminary operation have been implemented, it can perform a sorting operation under the event count rate up to 1 MHz per channel, and support four channels in total, count rate up to 4 MHz. The performance of this algorithm has been verified by pulse generator and <sup>22</sup>Na radiation source, which can sort the events with chaotic order into chronological order completely. The application of this algorithm provides not only an efficient solution for selection of coincidence events, but also a design of electronic circuit with a small-scale structure.
Weihuan Li,Quan Zhang,Xiangqiang Kong,Chunxia Wu,Xiuling Ma,Hui Zhang,Yanxiu Zhao 한국식물학회 2009 Journal of Plant Biology Vol.52 No.2
Vacuolar Na+/H+ antiporters catalyze the exchange of Na+ for H+ across vacuolar membranes and compartmentalize Na+ into vacuoles. They play important roles in cellular pH and Na+ homeostasis. The SsNHX1 gene was previously cloned from a typical euhalophyte, Suaeda salsa. Its cloning revealed another N-terminus truncated transcript, SsNHX2. This potentially alternative splicing variant was truncated from the 49 amino acid residues (aa) of the N terminus of SsNHX1. To compare their degree of salt tolerance, we over-expressed SsNHX1 and SsNHX2 in Arabidopsis. Southern and northern blot analyses showed that both genes had been integrated into that genome and had expressed in several lines. Two types of transgenic plants grew more vigorously than the wild type (WT) under salt stress, but no remarkable differences were found between those SsNHX1 and SsNHX2 transformants. Physiological analyses also supported this phenotype. Both fresh and dry weights of the transgenics as well as their accumulations of Na+ and K+ under salinity were much higher than that of WT, but differences were not significant between SsNHX1 and SsNHX2 plants for any of those parameters. These results suggest that SsNHX2 is a functional Na+/H+ antiporter like SsNHX1 and their levels of salt tolerance are similar.
Evaluation of energy correction algorithm for signals of PET in heavy-ion cancer therapy device
Niu, Xiaoyang,Yan, Junwei,Wang, Xiaohui,Yang, Haibo,Ke, Lingyun,Chen, Jinda,Du, Chengming,Zhang, Xiuling,Zhao, Chengxin,Kong, Jie,Su, Hong Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.1
In order to solve the contradiction between requirements of high sampling rate for acquiring accurate energy information of pulses and large amount of data to be processed timely, the method with an algorithm to correct errors caused by reducing the sampling rate is normally used in front-end read-out system, which is conductive to extract accurate energy information from digitized waveform of pulse. The functions and effects of algorithms, which mainly include polynomial fitting with different fitting times, double exponential function fitting under different sampling modes, and integral area algorithm, are analyzed and evaluated, and some meaningful results is presented in this paper. The algorithm described in the paper has been used preliminarily in a prototype system of Positron Emission Tomography (PET) for heavy-ion cancer therapy facility.