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      • KCI등재

        Structural Behavior Analysis of UHPC Hybrid Tower for 3-MW Super Tall Wind Turbine Under Rated Wind Load

        Xiang-Guo Wu,Xuesen Zhang,Hom Bahadur Bhattarai,황현종,Jing Yang,강순필 한국콘크리트학회 2022 International Journal of Concrete Structures and M Vol.16 No.6

        Based on the conceptual design of an advanced wind turbine tower system, use of ultra-high-performance cementitious composites material with compressive strength of 200 MPa (UHPC-200) is proposed to ensure high durability and ductility of the UHPC hybrid wind turbine tower. Key design parameters are proposed for the structural design of a 3-MW wind turbine. The material properties, mixing compositions, simplified constitutive relationship, and model parameters are outlined. Using nonlinear finite element analysis, the effects of wall thickness, wall thickness ratio, and prestressing tendon on the structural performance including the longitudinal stress field, lateral displacement, stress concentration at the transition zone between the middle and bottom segments are evaluated. Based on the stress-field analysis, the design limitation of the segmental wall thickness and its ratio is recommended. The numerical results show that the tower with the wall thickness ratio of 2:3 (i.e., thickness 200–300 mm) with prestressing tendons is an optimal design for the UHPC hybrid tower. The results of this study can be used as a reference for the engineering design of a new type of UHPC hybrid wind turbine tower.

      • KCI등재

        Theoretical study of UHPCC composite column behaviors under axial compression

        Xiang-Guo Wu,Ruofei Zou,Xinyu Zhao,Qun Yu 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.55 No.5

        To improve the durability and service life of reinforced concrete column such as bridge piers, an advanced composite column made of Ultra High Performance Cementitious Composites (UHPCC) permanent form is proposed. Based on elasticity plasticity theory, axial compression behavior of the composite column was studied theoretically. The first circumferential cracking load and ultimate limit loading capacity are derived for the composite column. Short composite column compression tests and numerical simulations using FEM method were carried out to justify the theoretical formula. The effects of UHPCC tube thickness on the axial compression behavior were studied. Using the established theoretical model and numerical simulation, the large dimension composite columns are calculated and analyzed with different UHPCC tube thickness. These studies may provide a reference for advanced composite column design and application.

      • KCI등재

        Flexural behavior of UHPC-RC composite beam

        Xiang-Guo Wu,Yang Lin 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.22 No.2

        In order to evaluate the effects of U shape ultra high performance concrete (UHPC) permanent form on the behaviors of Reinforced Concrete (RC) beam, a full scale RC composite beam is designed and tested with U shape UHPC permanent form and a reference RC beam with same parameters is tested simultaneously for comparison. The effects of the permanent form on the failure mode, cracking strength, ultimate capacity and deformation are studied. Test results shows that the contributions of the U shape UHPC permanent form to the flexural cracking behaviors of RC beam are significant. This study may provide a reference for the design of sustainable RC beam with high durable UHPC permanent form.

      • KCI등재

        Production of isophthalic acid from m-xylene oxidation under the catalysis of the H3PW12O40/carbon and cobalt catalytic system

        Xiang-li Long,Zhi-hao Wang,San-qiang Wu,Shi-ming Wu,Hai-feng Lv,Wei-kang Yuan 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.1

        Isophthalic acid (IPA) is commercially produced from m-xylene oxidation with the catalysis of thehomogeneous Co–Mn–Br catalyst system. In this study, a catalytic system consisting of HPW/C and Co(II)has been put forward to oxidize m-xylene (MX) to IPA. The experimental results prove that the HPW/Cand Co catalytic system is capable of catalyzing the oxidation of MX to IPA, which can obtain a higher MXconversion and IPA concentration than the homogeneous H3PW12O40/Co(OAc)2/Mn(OAc)2 catalyticsystem. The heterogeneous catalytic system is also advantageous over the homogeneous catalyticsystem in the inhibition of the oxidation of acetic acid and IPA. The optimal amount of phosphotungsticacid supported on carbon is 7.5% (wt). The best dosage of HPW/C is 15 g l-1. The optimum Co(II)concentration in the catalytic system for IPA production is 0.064% (wt). The best HPW/C activationtemperature is 220℃ .

      • Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

        Wu, Xiang-Mei,Liu, Xing,Jiao, Qing-Fang,Fu, Shao-Yue,Bu, You-Quan,Song, Fang-Zhou,Yi, Fa-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

        Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

      • KCI등재

        Novel AgCl/Ag2SO3 Hybrids as a Visible-Light-driven Photocatalyst: Preparation, Characterization, and Degradation of Rhodamine-B and Methyl Orange

        Xiang-Feng Wu,Yi-Jin Wang,Zuo-Lin Cao,Yan-Mei Feng,Hui Li,Chen-Xu Zhang,Jun-Zhang Su,Jia-Rui Zhang,Yi-Wei Wang,Kai-Yuan Wang,Guo-Wen Sun 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.7

        The novel AgCl/Ag2SO3 hybrids as an efficient photocatalyst had been fabricated by an in situ synthetic method. The correlations between the structure and the photocatalytic properties of the as-fabricated hybrids were analyzed. Experimental results exhibited that with increasing the amount of Ag2SO3, the degradation rate of the as-obtained samples was firstly increased and then decreased under the visible light irradiation. When the mass ratio of AgCl to Ag2SO3 was 1:2, in 30?min, it displayed the highest degradation rate of 99.2% for rhodamine-B, which was obviously higher than 46.1, 60.5, and 14.6% of pure AgCl, Ag2SO3, and TiO2 (P25), respectively. Similar results could be found in degradation of methyl orange. It had the maximum of 97.4% in 90?min, which was higher than 55.2, 48.7, and 12.7% of pure AgCl, Ag2SO3, and P25, respectively. Moreover, the as-prepared hybrids possessed the enhanced separation and transfer of photo-generated electron?hole pairs compared to the pure samples. In addition, the holes and superoxide radicals played the dominant role and the hydroxyl radicals played the secondary role during the process of photocatalytic degradation.

      • KCI등재

        Flexural behavior of precast concrete wall – steel shoe composite assemblies with dry connection

        Xiang-Guo Wu,Xinlei Xia,Thomas H.-K. Kang,Jingcheng Han,김창수 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.29 No.4

        This study aimed to investigate the flexural behavior of precast concrete (PC) wall – steel shoe composite assemblies with various dry connection details at mid-span. Flexural tests were performed for five scenarios. Test parameters included the width of test specimens, arrangement of steel shoe connectors, and use of structural adhesive or waterproof tape at the mid-span joint. The test results showed that the PC wall – steel shoe composite assemblies joined at mid-span showed flexural damage patterns combined with rotational deformation, and the structural performance was satisfactory regardless of the arrangement of steel shoe connectors. Considering the two deformation components (flexural deformation by bending and rotational deformation due to joint opening), a theoretical model was proposed to analyze flexural strength and joint opening, and the simple model gave good predictions with acceptable accuracy.

      • KCI등재

        Structural behaviors of sustainable hybrid columns under compression and flexure

        Wu Xiang-Guo,Hu Qiong,Zou Ruofei,Zhao Xinyu,Yu Qun 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.52 No.5

        Structural behaviors of a sustainable hybrid column with the ultra high performance cementitious composites (UHPCC) permanent form under compression and flexure were studied. Critical state and failure stage characters are analyzed for large and small eccentricity cases. A simplified theoretical model is proposed for engineering designs and unified formulas for loading capacity of the hybrid column under compression and flexure loads are derived, including axial force and moment. Non-linear numerical analysis is carried out to verify the theoretical predictions. The theoretical predictions agree well with the numerical results which are verified by the short hybrid column tests recursively. Compared with the traditional reinforced concrete (RC) column, the loading capacity of the sustainable hybrid column is improved significantly due to UHPCC confinements.

      • Proteomic analysis of novel targets associated with TrkA-mediated tyrosine phosphorylation signaling pathways in SK-N-MC neuroblastoma cells

        Wu, Fang-Xiang,Ressom, Habtom,Dunn, Michael J Blackwell Publishing Inc 2013 Proteomics Vol.13 No.2

        <P>Tropomyosin-related kinase A (TrkA) is a receptor-type protein tyrosine kinase and exploits pleiotypic roles via nerve growth factor (NGF)-dependent or NGF-independent mechanisms in various cell types. Here, we showed that the inhibition of TrkA activity by GW441756 resulted in the suppression of tyrosine phosphorylation of cellular proteins including extracellular signal-regulated protein kinase (ERK) and c-Jun N-terminal kinase (JNK). To find novel targets associated with TrkA-mediated tyrosine phosphorylation signaling pathways, we investigated GW441756 effects on TrkA-dependent targets in SK-N-MC neuroblastoma cells by proteomic analysis. The major TrkA-dependent protein spots controlled by GW441756 were determined by PDQuest image analysis, identified by MALDI-TOF MS and MALDI-TOF/TOF MS/MS, and verified by 2DE/Western blot analysis. Thus, we found that most of the identified protein spots were modified forms in a normal condition, and their modifications were regulated by TrkA activity. Especially, our results demonstrated that the modifications of α-tubulin and heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C1/C2) were significantly upregulated by TrkA, whereas α-enolase modification was downregulated by TrkA, and it was suppressed by GW441756, indicating that TrkA activity is required for their modifications. Taken together, we suggest here that the major novel TrkA-dependent targets such as α-tubulin, hnRNP C1/C2, and α-enolase could play an essential role in TrkA-mediated tyrosine phosphorylation signaling pathways via regulation of their posttranslational modifications.</P>

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