Improved Ethanol Production in Jerusalem Artichoke Tubers by Overexpression of Inulinase Gene in Kluyveromyces marxianus

WenJie Yuan,Xinqing Zhao,Li-Jie Chen,Feng-Wu Bai 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4

Ethanol production from Jerusalem artichoke tubers through a consolidated bioprocessing (CBP) strategy using the inulinase-producing yeast Kluyveromyces marxianus is an economical and competitive than that from a grainbased feedstock. However, poor inulinase production under ethanol fermentation conditions significantly prolongs the fermentation time and compromises ethanol productivity. Improvement of inulinase activity appears to be promising for increasing ethanol production from Jerusalem artichoke tubers by CBP. In the present study, expression of the inulinase gene INU with its own promoter in K. marxianus (K/INU2) was explored using the integrative cassette. Overexpression of INU was explored using chromosome integration via the HO locus of the yeast. Inulinase activity and ethanol were determined from inulin and Jerusalem artichoke tubers under fed-batch operation. Inulinase activity was 114.9 U/mL under aerobic conditions for K/INU2, compared with 52.3 U/mL produced by the wild type strain. Importantly, inulinase production was enhanced in K/INU2 under ethanol fermentation conditions. When using 230 g/L inulin and 220 g/L Jerusalem artichoke tubers as substrates, inulinase activities of 3.7 and 6.8 U/mL,respectively, were measured using K/INU2, comparing favorably with 2.4 and 3.1 U/mL, respectively, using the wide type strain. Ethanol concentration and productivity for inulin were improved by the recombinant yeast to 96.2 g/L and 1.34 g/L/h, respectively, vs 93.7 g/L and 1.12 g/L/h,respectively, by the wild type strain. Ethanol concentration and productivity improvements for Jerusalem artichoke tubers were 69 g/L and 1.44 g/L/h, respectively, from the recombinant strain vs 62 g/L and 1.29 g/L/h, respectively,from the wild type strain.

Novel perpendicularly cross-rectangular CuO architectures: Controlled synthesis, enhanced photocatalytic activity and catalytic thermal-decomposition of NH4ClO4

Hanmei Hu,Xinqing Ge,Qiang Zheng,Chonghai Deng 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.11

Novel perpendicularly cross-rectangular CuO architectures have been successfully fabricated on a large scale by a facile microwave-assisted chemical aqueous route. The as-synthesized CuO products were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), high resolution transmission electron microscopy (HRTEM) and UV-vis absorption spectroscopy. An individual CuO microstructure is mainly assembled by two rectangle-shaped nanosheets with different sizes, which is perpendicularly intersected through the center. A possible formation mechanism of perpendicularly cross-rectangular CuO architectures was proposed based on the comparative experimental results. The prepared CuO nanoarchitectures exhibited excellent photocatalytic activity for the decolorization of Rhodamine B (RhB) under visible light irradiation. Simultaneously, the prepared CuO products, acting as an additive, also showed effective catalytic activity on the thermal decomposition of ammonium perchlorate (NH4ClO4).

중국전통 전지공예와 현대도자장식의 융합에 대한 연구

이신청,위정기,엄붕,김원석,노혜신,김성민,Xinqing, Li,Zhengqi, Wei,Peng, Yan,Kim, Won-Suk,Ro, Hae-Sin,Kim, Sung-Min 한국디지털정책학회 2020 디지털융복합연구 Vol.18 No.9

본 연구는 중국전통 전지공예와 현대도예의 융합을 시론적으로 다룬 것이다. 전지공예는 중국인이 오랫동안 일상에서 즐겨온 팝아트적 예술이며, 현대도자는 이로부터 창작의 소재와 모티브를 공급받아 작가의 창의성을 자극하며 도예의 새로운 길을 열고 있다. 순수한 평면예술인 전지공예는 도자기를 만나 3차원 공간으로 전이되어 새로운 예술언어로 번역되었으며 도자재료는 그 가소성과 수용성으로 말미암아 전지공예 내포하고 있는 음양허실의 조화는 물론 전지작품에 대한 새로운 해석까지 가능하게 하였다. 본 연구는 이와 같은 양자의 융합에 대한 경향성과 가능성을 보여주고 있다. This study is an introductory study on Convergence of Traditional Chinese Paper-cutting Art and Contemporary Ceramic Decoration. As a part of Chinese folk art, now newly developed by the convergence of contemporary ceramic ar, today it promotes artists' creativity and find a alternative way for ceramic art. This mainly explore transition of 2 dimentional paper-cutting art into ceramic art. As a part of convergence art, this study suggest combination of 4 elements yin-yang-true-false with understanding of its history and contemporary status, and towards aesthetic values.

Antifungal activity of Streptomyces albidoflavus L131 against the leaf mold pathogen Passalora fulva involves membrane leakage and oxidative damage

Chao Chen,Yumei Wang,Chun Su,Xinqing Zhao,Ming Li,Xiaowei Meng,김영우,양승환,Yushu Ma,Dong-Zhi Wei,서주원 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1

Passalora fulva (or Fulvia fulva) is the causalmicroorganism of tomato leaf mold, the outbreak of whichoccurs worldwide in greenhouse especially when humidityis high. However, studies on antifungal agents of P. fulvaare still very limited. In this study, a marine-derivedStreptomyces albidoflavus strain L131 showing potentinhibitory activities against P. fulva was identified andcharacterized. The active antifungal components wereobtained, and studies on the antifungal mechanisms of thecrude extract showed that the antifungal metabolites ofL131 caused damage of hyphae and spore development, aswell as plasma membrane of P. fulva. In addition, accumulationof endogenous reactive oxygen species of the leafpathogen was also observed after treatment by cultureextracts of L131. To our knowledge, this is the first reporton the studies of the antifungal mechanisms againstP. fulva, which benefit further development of biocontrolagent against tomato leaf mold disease.

Antifungal activity of Streptomyces albidoflavus L131 against the leaf mold pathogen Passalora fulva involves membrane leakage and oxidative damage

Chen, Chao,Wang, Yumei,Su, Chun,Zhao, Xinqing,Li, Ming,Meng, Xiaowei,Jin, Yingyu,Yang, Seung-Hwan,Ma, Yushu,Wei, Wei,Joo-Won, Suh 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.1

Passalora fulva (or Fulvia fulva) is the causal microorganism of tomato leaf mold, the outbreak of which occurs worldwide in greenhouse especially when humidity is high. However, studies on antifungal agents of P. fulva are still very limited. In this study, a marine-derived Streptomyces albidoflavus strain L131 showing potent inhibitory activities against P. fulva was identified and characterized. The active antifungal components were obtained, and studies on the antifungal mechanisms of the crude extract showed that the antifungal metabolites of L131 caused damage of hyphae and spore development, as well as plasma membrane of P. fulva. In addition, accumulation of endogenous reactive oxygen species of the leaf pathogen was also observed after treatment by culture extracts of L131. To our knowledge, this is the first report on the studies of the antifungal mechanisms against P. fulva, which benefit further development of biocontrol agent against tomato leaf mold disease.

Adjustable and extensible hexagonal acoustic metamaterial cell combining multiple parallel Helmholtz resonators with optional apertures

Fei Yang,Shaohua Bi,Xinmin Shen,Xiaocui Yang,Xinqing Wang,Xiangpo Zhang,Jinhong Xue,Xiaonan Zhang,Qin Yin,Juying Dai,Wenqiang Peng,Jingwei Zhu,Junhong Ren 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.12

To prevent noise pollution, a hexagonal acoustic metamaterial cell combining multiple parallel Helmholtz resonators with optional apertures is proposed. There were 6 trapezoidal chambers and 6 triangular chambers, and each front panel had 6 different apertures, which meant that there were 6 12 = 2176782336 possible permutations. The distribution of sound pressures obtained by acoustic finite element simulation revealed the acoustic absorption mechanism, which provided effective guidance to alter the absorption capacity. For certain scenarios, the acoustic absorption performance was optimized by the joint combination of artificial neural network and acoustic finite element simulation. Through manufacturing and testing the sample, actual average acoustic absorption coefficients were achieved at 0.6733, 0.7296, 0.8785 and 0.7065 for the target frequency ranges 350-950 Hz, 400-1000 Hz, 500-800 Hz and 350-700 Hz, respectively, with total thickness 40 mm. The tunable acoustic absorption property proved that the hexagonal acoustic metamaterial cell was appropriate for noise reduction with variable frequency ranges.

Metal-Doped In2O3 Nanosphere Arrays with Enhanced Gas-Sensing Property

Xuanji Zhang,Ni Deng,Xianjia Chen,Yanting Yang,Jing Li,BO HONG,DINGFENG JIN,Xiaoling Peng,XINQING WANG,Hongliang Ge,HONGXIAO JIN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.3

In2O3 and metal-doped (Ni or Ce) In2O3 mesoporous three-dimensional (3D) nanospheres arrays were synthesized via nanocasting using mesoporous silica as hard templates. Effects of Ni or Ce doping on the structure, optical and gas-sensing properties of the In2O3 nanospheres were investigated. Both the undoped and the doped In2O3 nanospheres showed single-phase structure without any impurity. The nanospheres were about 20 nm in size and they stacked closely to formrigid 3D mesoporous structure. Achange in the value of optical band gap was observed upon metal doping. The room temperature photo luminescence behavior also showed some differences between pure and doped In2O3. Compared with pure In2O3 nanospheres, the metal-doped In2O3 exhibited superior response, fast recovery and good selectivity to ethanol. The enhanced gas-sensing properties might be related to the doping of metal ion and its effective contribution towards the oxygen vacancies, conductivity and crystallite size of the grains.

SYNTHESIS OF MAGNETIC SBA-15 AND Fe–SBA-15 MESOPOROUS NANOCOMPOSITES WITH COBALT FERRITES

HONGXIAO JIN,LIANG LI,MIN CHEN,JINGCAI XU,BO HONG,BAO HUANG,DINGFENG JIN,HONGLIANG GE,XINQING WANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.3

A new nanocomposite based on SBA-15 mesoporous materials combined with Fe_2O_3 and CoFe_2O_4 nanoparticles was prepared via sol–gel growth. The nanostructures and magnetic properties of the SBA-15 nanocomposite were investigated by X-ray diffraction topography, high resolution transmission electron microscopy, and vibrating sample magnetometer. Results indicate that α-Fe_2O_3 nanoparticles are present in the frame or micropores of SBA-15 (denoted as Fe–SBA-15 below) and that CoFe_2O_4 nanoparticles are confined in the mesoporous channels of Fe–SBA-15. Our results also reveal that the addition of CoFe_2O_4 and α-Fe_2O_3 magnetic nanoparticles critically affects their magnetic properties. The saturation magnetization of the SBA-15 nanocomposite is attributed to ferrimagnetic CoFe_2O_4 nanoparticles and antiferromagnetic α-Fe_2O_3 nanoparticles, whereas the coercivity increases with the content of CoFe_2O_4. Moreover, the presence of the couple exchange interaction between the magnetic nanoparticles is confirmed, which can enhance the magnetic properties of the SBA-15 nanocomposite.

An Experimental Study on Spray Transient Characteristics of Fuel Containing Co2 Using a High Speed Camera

( Jin Xiao ),( Jun Jun Ma ),( Zhen Huang ),( Xinq I Qiao ) 한국액체미립화학회 2005 한국액체미립화학회 학술강연회 논문집 Vol.2005 No.-

Injection of fuel containing CO2 involves dissolving the gas into fuel prior to injection, which has potential to reduce the NOx and soot emissions in diesel engine. This paper presents an experimental study on the flash boiling and atomization process of transient spray of fuel containing CO2 spray by means of high speed imaging technology. Experiments were performed under atmospheric conditions on a diesel hole-type nozzle. The effect of injection pressure on the flash boiling and atomization process were evaluated, especially in the early stage of injection. The transient characteristics of the spray, such as spray-angle and spray tip penetration were also investigated. The images clearly display the developing process of flashing boiling spray . Experimental results show that fuel atomization will improve greatly when the concentration of dissolved CO2 in the fuel is larger enough. New insight into the flash boiling and atomization process of the fuel containing CO2 spray was obtained and possible mechanism to explain the phenomena was proposed.