Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

Lee, Jong-Uk,Cheong, Hyun Sub,Shim, Eun-Young,Bae, Da-Jeong,Chang, Hun Soo,Uh, Soo-Taek,Kim, Young Hoon,Park, Jong-Sook,Lee, Bora,Shin, Hyoung Doo,Park, Choon-Sik BioMed Central 2017 Respiratory research Vol.18 No.-

<P><B>Background</B></P><P>Idiopathic pulmonary fibrosis (IPF) is characterized by the complex interaction of cells involved in chronic inflammation and fibrosis. Global gene expression of a homogenous cell population will identify novel candidate genes.</P><P><B>Methods</B></P><P>Gene expression of fibroblasts derived from lung tissues (8 IPF and 4 controls) was profiled, and ontology and functional pathway were analyzed in the genes exhibiting >2 absolute fold changes with <I>p</I>-values < 0.05. <I>CCL8</I> mRNA and protein levels were quantified using real-time PCR and ELISA. <I>CCL8</I> localization was evaluated by immunofluorescence staining.</P><P><B>Results</B></P><P>One hundred seventy eight genes differentially expressed and 15 genes exhibited >10-fold change. Among them, 13 were novel in relation with IPF. <I>CCL8</I> expression was 22.8-fold higher in IPF fibroblasts. The levels of <I>CCL8</I> mRNA and protein were 3 and 9-fold higher in 14 IPF fibroblasts than those in 10 control fibroblasts by real-time PCR and ELISA (<I>p</I> = 0.022 and <I>p</I> = 0.026, respectively). The <I>CCL8</I> concentrations in BAL fluid was significantly higher in 86 patients with IPF than those in 41 controls, and other interstitial lung diseases including non-specific interstitial pneumonia (<I>n</I> = 22), hypersensitivity pneumonitis (<I>n</I> = 20) and sarcoidosis (<I>n</I> = 19) (<I>p</I> < 0.005, respectively). Cut-off values of 2.29 pg/mL and 0.43 pg/mL possessed 80.2 and 70.7% accuracy for the discrimination of IPF from NC and the other lung diseases, respectively. IPF subjects with <I>CCL8</I> levels >28.61 pg/mL showed shorter survival compared to those with lower levels (<I>p</I> = 0.012). <I>CCL8</I> was expressed by α-SMA-positive cells in the interstitium of IPF.</P><P><B>Conclusions</B></P><P>Transcriptome analysis identified several novel IPF-related genes. Among them, <I>CCL8</I> is a candidate molecule for the differential diagnosis and prediction of survival.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12931-016-0493-6) contains supplementary material, which is available to authorized users.</P>

지역 경제 사례를 통한 경제교육자료 개발

정창욱 한국경제교육학회 2005 경제교육연구 Vol.12 No.2

이식신의 분절성 사구체경화

정현주,오창권,김건욱,김유선,박기일,최인준 大韓移植學會 1994 Korean Journal of Transplantation Vol.8 No.1

Cu(Mg) 박막의 Time Dependent Dielectric Breakdown 특성 평가

안정욱,황상수,박영배,주영창 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.2

Electrical properties and time dependent dielectric breakdown (TDDB) characteristics of Cu (0.7 at.% Mg) alloy films are investigated to improve the reliability of Cu interconnects used in microelectronic devices. After Cu(Mg) films are annealed in vacuum at 400℃ for 30 min, resistivity decreases from 3.4 to 2.0 μΩcm and, thin and continuous MgO layers form both on Cu(Mg) film surface and on Cu(Mg)/SiO₂ interface. Metal-Oxide-Silicon (MOS) capacitors composed of Cu(Mg) film deposited on thermal oxide grown on Si wafer are subjected to bias temperature stressing at 180 through 220℃ under electric fields of 1.5 through 2.6 MV/cm. Median time to failure (MTTF) and activation energy of Cu(Mg)/SiO₂ are much larger than those of pure Cu/SiO₂. Cu drifts into dielectric are inhibited by the continuous Mg oxide formed at Cu(Mg)/SiO₂ interface which improve interfacial adhesion and finally lead to better interconnect reliabilities. (Received October 29, 2004)

기종성 신우신염 1례

이재욱,구정태,서정일,양창헌,이정호,이동철,이영현,이활,이경섭 동국대학교 경주대학 1997 東國論集 Vol.16 No.1

기종성 신우신염은 주로 당뇨병환자나 폐쇄성 요로질환을 가진 환자에 발생하며 특징적으로 신실질 및 그 주위조직에 가스를 형성하면서 심한 조직괴사를 일으키는 매우 드문 급성 화농성 신감염으로 사망률이 높은 질환이다. 이 질환은 1989년 Kelly와 MacCallum에 의해 처음 보고된 이래 외국문헌에 약 90례 정도가 보고되어 있으며 국내에는 28례가 보고되어 있다. 수액 및 전해질 보충, 인슐린 투여를 통한 적절한 혈당조절 및 항생제 투여등의 내과적 치료와 절개배농 및 신적출술등의 수술적 치료 방법이 있다. 내과적 치료로 가스의 감소가 없는 경우에는 즉각적인 수술적 치료를 시행하여야하므로 이 가스변화에 대한 추적관찰이 중요하다 하겠다. 본 저자들은 당뇨병환자에서 발생한 기종성 신우신염 1례를 내과적 요법을 치험하였기에 문헌고찰과 함께 보고하고자 한다. Emphysematous pyelonephritis is rare, life-threatening infection of the renal parenchyma and perirenal tisseue. This disease is characterized by the production of intrarenal and perirenal gas and is frequently encountered in patients with diabetes mellitus or urinary obstruction. We experienced a case of emphysematouse pyelonephritis in a 62 years old women with poorly controlled diabetes mellitus who had been managed with medical theraphy. So we report this case with a review of the referenced literatures.

SNMP를 이용한 효율적인 시스템 모니터링

김태수,정창영,김동억,김용석 江原大學校 産業技術硏究所 2005 産業技術硏究 Vol.25 No.A

SNMP is a standard protocol for management of networking devices. Nowadays, most computer systems have capability to act as SNMP agents. In this paper, we describe our system monitoring software based on SNMP. It consists of a monitoring server, SNMP agents, and client programs. The monitoring server collects status information from the SNMP agents running on the monitoring target graphical display. We developed two version of clients, Java based standalone program and Web based program. Since most known SNMP packages are too big and heavy, we developed an efficient version of SNMP library for out system monitoring

스테로이드로 유발된 쥐의 근골격계 변화에대한 성장호르몬의 치료 효과

최기섭,박창일,정우성,이상건,박용욱 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.5

Multiple myeloma 환자에서 Bortezomib치료 후 발생한 마비성 장폐색증 1례

김준영,이원식,손창학,박성길,진한영,박석주,조영완,정은욱,강명주,박정하,주영돈 인제대학교 2006 仁濟醫學 Vol.27 No.-

Bortezomib is the first clinically available proteasome inhibitor that is clinically effective in the treatment of multiple myeloma. A proteasome inhibitor acts through multiple mechanisms to arrest tumor growth, tumor spread, and angiogenesis. The main adverse effects of bortezomib are gastrointestinal symptoms, cytopenia, fatigue, and peripheral neuropathy. To date, severe paralytic ileus has not been reported as a toxic effect of bortezomib treatment in multiple myeloma. Bortezomib is a novel agent that has only been used clinically for 30 months, so a need exists to further evaluate its toxicity. We report a case of grade Ⅲ (NCI CTCAE v3.0) or grade Ⅳ(SWOG toxicity criteria) paralytic ileus in a 65-year-old man with relapsed multiple myeloma who underwent one cycle of single-agent bortezomib treatment scheduled in a 21-day cycle (1.3 mg/㎥ as a single i.v. bolus on days 1, 4, 8, and 11).

Predictability of Early Change in Derived Neutrophil to Lymphocyte Ratio A in Patients with Advanced Non-small Cell Lung Cancer Under Immune Checkpoint Inhibitors

( Jeong Uk Lim ),( Chang Dong Yeo ),( Hye Seon Kang ),( Ju Sang Kim ),( Chan Kwon Park ),( Jin Woo Kim ),( Seung Joon Kim ),( Sang Haak Lee ) 대한결핵 및 호흡기학회 2020 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.128 No.-

Purpose While association between systemic inflammation and disease progression is suggested, we assessed potential predictability of early changes in neutrophil to lymphocyte ratio (NLR) and derived NLR (dNLR) in NSCLC under ICI. Method Among a multicenter cohort of lung cancer, patients with advanced (stage IIIB-IV) NSCLC who were under ICI between January 2016 and December 2019 were enrolled from six university hospitals. Complete blood count was measured just before 1st cycle of ICI and 2nd cycle of ICI. Differences in NLR, and dNLR were measured. When the increase in NLR was equal or more than 1, the patients were classified into the “NLR increase group” if otherwise, they were classified in to the “NLR non-increase group.” Likewise, when the increase in dNLR was equal or more than 1, the patients were classified into the “dNLR increase group”, if not, they were classified into the “dNLR non-increase group”. Results Total of 89 patients were selected for evaluation. Median progression free survival (PFS) was significantly longer for the NLR non-increase group when compared to the NLR increase group (9.5 vs 2.6 months, P<0.001). Derived NLR increase group showed significantly shorter median PFS when compared to the dNLR non-increase group (4.2 vs 9.2 months, P=0.001) (Figure). Using cox regression hazard model, association with PFS was analyzed. In the model 1, in which NLR increase was entered, increase ≥ 1 in NLR showed significant association (HR=3.007, 95% CI =1.610-5.617, P=0.001). In the model 2, in which dNLR increase was entered in the multivariate analysis, increase ≥ 1 in dNLR showed significant association (HR=2.601, 95% CI=1.331-5.079, P=0.005) (Table). Conclusion While early change in dNLR and NLR were shown to have prognostic value in patients undergoing immunotherapy, using multiple measurement points, they can be more accurate and comprehensive biomarkers in predicting ICI response.

The Effect of Choline Acetyltransferase Genotype on Donepezil Treatment Response in Patients with Alzheimer’s Disease

Lee, Kang Uk,Lee, Jung Hie,Lee, Dong Young,Youn, Jong Chul,Kim, Jeong Lan,Moon, Seok Woo,Kim, Bong-Jo,Ryu, Seung-Ho,Kim, Moon Doo,Lee, Chang-Uk,Lee, Nam-Jin,Chang, Sung Man,Kim, Young Hoon,Kim, Do Hoo Korean College of Neuropsychopharmacology 2015 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.13 No.2

<P><B>Objective</B></P><P>We examined the difference in responses to donepezil between carriers and non-carriers of the A allele at the +4 position of the choline acetyltransferase (ChAT) gene in Koreans.</P><P><B>Methods</B></P><P>Patients who met the criteria for probable Alzheimer’s disease (AD) (n=199) were recruited. Among these, 145 completed the 12-week follow-up evaluation and 135 completed the 26-week scheduled course. Differences and changes in the Korean version of the mini-mental state examination (MMSE-KC) score, Korean version of the Consortium to Establish a Registry for Alzheimer’s Disease Neuropsychological Assessment Battery (CERAD-K[N]) wordlist subtest score (WSS), CERAD-K(N) total score (TS), and the Korean version of geriatric depression scale (GDS-K) score between baseline and 12 weeks or 26 weeks were assessed by the Student’s <I>t</I>-test.</P><P><B>Results</B></P><P>At 12 weeks, the changes in the MMSE-KC score, CERAD-K(N) WSS, and CERAD-K(N) TS from baseline were not significant between ChAT A allele carriers and non-carriers; however, at 26 weeks, these changes were significantly larger in ChAT A allele carriers than in non-carriers (<I>p</I>=0.02 for MMSE-KC and <I>p</I>=0.03 for CERAD-K(N) WSS respectively).</P><P><B>Conclusion</B></P><P>Our findings in this study suggested that presence of the A allele at the +4 position of ChAT might positively influence the treatment effect of donepezil in the early stages of AD in Koreans.</P>