葡萄汁加酸棗仁湯의 스트레스 해소효과에 대한 臨床的 硏究

李仲徽,崔昇勳,吳旼錫,김재식,윤상원 대전대학교 한의학연구소 2003 한의학연구소 논문집 Vol.12 No.1

Clinical studies were carried out 29 cases through administration of Sanjointang added Grape Juice(ST+GJ) from December 1st 2002 to December 30th 2002. The results were summarized as follows; 1. There were significant decreases in physical symptoms of fatigue from 4.34±2.70 to 2.34±1.78 by administration of ST+GJ. 2. There were significant decreases in mental symptoms of fatigue from 5.10±2.74 to 3.76±2.23 by administration of ST+GJ. 3. There were significant decreases in neuro-sensory symptoms of fatigue from 3.66±2.13 to 2.69±2.07 by administration of ST+GJ. 4. There were significant decreases in total subjective symptoms of fatigue from 13.14±6.18 to 8.79±4.63 by administration of ST+GJ. 5. There were significant decreases in VAS of fatigue ratio, but not significant changes in VAS of concentration ratio by administration of ST+GJ. According to the results, we could suggest that ST+GJ is able to be applied to the relaxion of stress.

Protopanaxadiol modulates LPS-induced inflammatory activity in murine macrophage RAW264.7 cells

Lee, Whi-Min,Kim, Sung-Dae,Kim, Kil-Soo,Song, Yong-Bum,Kwak, Yi-Seong,Cho, Jae-Youl,Park, Hwa-Jin,Oh, Jae-Wook,Rhee, Man-Hee The Korean Society of Ginseng 2006 Journal of Ginseng Research Vol.30 No.4

Protopanaxadiol (PPD) is a mixture of protopanaxadiol type saponins with a dammarane skeleton, from Korean red ginseng (Panax ginseng C.A. Meyer; Araliaceae). Korean ginseng is well-known herb to treat almost all kinds of diseases in Oriental medicine. This herb was particularly prescribed for treatment various inflammatory diseases, including rheumatoid arthritis, atherosclerosis, and diabetes mellitus, for centuries. To understand the efficacy of ginseng against inflammatory diseases, we aimed to show anti-inflammatory activities of the PPD in murine macrophage cell line, RAW264.7 cells using nitric oxide (NO) production assay and the expressions of pro-inflammatory cytokines, such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), and IL-6, and monocyte chemotactic protein-1 (MCP-1). We found that PPD saponin significantly blocked LPS ($1{\mu}g/ml$)-induced NO production in a dose-dependent manner. In addition, PPD abrogated the expressions of LPS-induced pro-inflammatory cytokines, such as IL-$1{\beta}$ and MCP-1. Moreover, cyclooxygenase (COX)-2, a critical enzyme to produce prostaglandin E2 (PGE2), was significantly inhibited by PPD in LPS-activated RAW264.7 cells. Taken together, these results suggested that anti-inflammatory efficacy of Korean red ginseng on inflammatory diseases is, at least, due to the NO inhibitory activity and the inhibition of the expressional level of inflammatory cytokines and/or mediators.

Davallialactone, isolated from Inonotus xeranticus, is apotent anti-inflammatory priciple with inhibiting Akt/PI3K pathway

RHEE man hee,LEE yong gyu,KIM joo young,BYEON se eun,KIM byung him,LEE ji yeon,LEE whi min,KIM sung dae 대한약학회 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.2

JAPANESS ENCEPHALITIS VIRUS ISOLAITON FROM MOSQUITOES OF KOREA

Lee, Ho Wang,Min, Bong Whi,Lee, Yung Whi 대한의학협회 1969 대한의학협회지 Vol.12 No.4

Inhibitory Activities of Red Ginseng Acidic Polysaccharide in Platelet Aggregation

Whi Min Lee,S.M. Kamruzzaman,Yong Bum Song,Jae Youl Cho,Hwa Jin Park,Man Hee Rhee 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1

Red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng (Panax ginseng C.A. Meyer), has been shown to have a variety of biological functions such as immunostimulating and anti-tumor activities. In the present study, we investigated whether RGAP inhibited ligand-induced platelet aggregation. The washed platelet-rich plasma was prepared from male SD rats with successive centrifugation. The platelets (10?/ml) were preincubated with 1 mM of CaCl₂for 2 min either in the presence or in the absence of RGAP (10 ~ 50 ㎍/ml) and were stimulated with collagen (2.5 ㎍/ml) and thrombin (0.1 U/ml). RGAP dose-dependently inhibited thrombin-induced platelet aggregation with IC50 value of 26.2±2.0 ㎍/ml. In collagen-induced platelet aggregation, RGAP inhibited the reaction with an IC50 value of 31.5±3.0 ㎍/ml. RGAP potently suppressed the intracellular calcium ion, which was stimulated by thrombin (0.1 U/ml). Among mitogen-activated protein kinase (MAPK) subtypes, the extracellular signal-regulated kinase (ERK) 1/2 and p38 MAPK were analyzed in the present study. RGAP inhibited the phosphorylation of ERK2 and p38 MAPK, which was activated by collagen (2.5 ㎍/ml). Finally, these results suggested that besides saponin fraction, RGAP take an important role in the preventive effect of Korean red ginseng against cardiovascular disease such as thrombosis and atherosclerosis.

Inhibitory Activities of Red Ginseng Acidic Polysaccharide in Platelet Aggregation

Lee, Whi-Min,Kamruzzaman, S.M.,Song, Yong-Bum,Cho, Jae-Youl,Park, Hwa-Jin,Rhee, Man-Hee The Korean Society of Ginseng 2008 Journal of Ginseng Research Vol.32 No.1

Red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng (Panax ginseng C.A. Meyer), has been shown to have a variety of biological functions such as immunostimulating and anti-tumor activities. In the present study, we investigated whether RGAP inhibited ligand-induced platelet aggregation. The washed platelet-rich plasma was prepared from male SD rats with successive centrifugation. The platelets $(10^8/ml)$ were preincubated with 1 mM of $CaCl_2$ for 2 min either in the presence or in the absence of RGAP $(10{\sim}50\;{\mu}g/ml)$ and were stimulated with collagen (2.5 ${\mu}g/ml$) and thrombin (0.1 U/ml). RGAP dose-dependently inhibited thrombin-induced platelet aggregation with $IC_{50}$ value of $26.2{\pm}2.0$ ${\mu}g/ml$. In collagen-induced platelet aggregation, RGAP inhibited the reaction with an $IC_{50}$ value of $31.5{\pm}3.0\;{\mu}g/ml$. RGAP potently suppressed the intracellular calcium ion, which was stimulated by thrombin (0.1 U/ ml). Among mitogen-activated protein kinase (MAPK) subtypes, the extracellular signal-regulated kinase (ERK) 1/2 and p38 MAPK were analyzed in the present study. RGAP inhibited the phosphorylation of ERK2 and p38 MAPK, which was activated by collagen (2.5 ${\mu}g/ml$). Finally, these results suggested that besides saponin fraction, RGAP take an important role in the preventive effect of Korean red ginseng against cardiovascular disease such as thrombosis and atherosclerosis.

Protopanaxadiol modulates LPS-induced inflammatory activity in murinemacrophage RAW264.7 cells

Whi Min Lee,Sung Dae Kim,Kil Soo Kim,Yong Bum Song,Yi Seong Kwak,Jae Youl Cho,Hwa Jin Park,Jae Wook Oh,Man Hee Rhee 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.4

Protopanaxadiol (PPD) is a mixture of protopanaxadiol type saponins with a dammarane skeleton, from Korean red ginseng (Panax ginseng C.A. Meyer; Araliaceae). Korean ginseng is well-known herb to treat almost all kinds of diseases in Oriental medicine. This herb was particularly prescribed for treatment various inflammatory diseases, including rheumatoid arthritis, atherosclerosis, and diabetes mellitus, for centuries. To understand the efficacy of ginseng against inflammatory diseases, we aimed to show anti-inflammatory activities of the PPD in murine macrophage cell line, RAW264.7 cells using nitric oxide (NO) production assay and the expressions of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6, and monocyte chemotactic protein-1 (MCP-1). We found that PPD saponin significantly blocked LPS (1 μg/ml)-induced NO production in a dose-dependent manner. In addition, PPD abrogated the expressions of LPS-induced pro-inflammatory cytokines, such as IL-1β and MCP-1. Moreover, cyclooxygenase (COX)-2, a critical enzyme to produce prostaglandin E2 (PGE2), was significantly inhibited by PPD in LPS-activated RAW264.7 cells. Taken together, these results suggested that anti-inflammatory efficacy of Korean red ginseng on inflammatory diseases is, at least, due to the NO inhibitory activity and the inhibition of the expressional level of inflammatory cytokines and/or mediators.

급성 심근경색 환자에서 예후 예측인자로 혈청 감마-글루타밀 트랜스퍼라제가 유용한가?

이장훈 ( Jang Hoon Lee ),채성철 ( Shung Chull Chae ),이현상 ( Hyun Sang Lee ),박용휘 ( Yong Whi Park ),류현민 ( Hyeon Min Ryu ),이순학 ( Soon Hak Lee ),배명환 ( Myung Hwan Bae ),양동헌 ( Dong Heon Yang ),박헌식 ( Hun Sik Park ) 대한내과학회 2007 대한내과학회지 Vol.72 No.3

목적: 혈청 감마-글루타밀 트랜스퍼라제(GGT)는 관상동맥 죽상경화반 내의 저밀도 지단백(LDL)의 산화과정을 촉매 하여 관상동맥 질환의 진행에 관여하며, 관상동맥 질환의 과거력이 있는 환자에서 심장사와 재경색의 독립적인 예후 예측인자로 알려져 있다. 저자는 관상동맥 질환의 과거력이 없는 급성 관상동맥 증후군 환자에서 예후 예측인자로서의 혈청 GGT의 효용성을 연구하였다. 방법: 흉통을 주소로 응급실을 방문하여 급성 심근경색으로 진단받은 환자의 혈청 GGT 값을 측정하여 응급실 방문당시 혈청 GGT 값이 정상범위(남자: 8-61 U/L; 여자: 5-31 U/L)에 있었던 192명(남/여=143/49, 평균 연령: 60.8±11.8세)의 환자를 대상으로 하여, 추적기간(16.5±10.8개월) 내 심장사건이 재발한 환자에서의 혈청 GGT 값을 심장사건이 없었던 환자의 혈청 GGT 값과 비교 하였다. 결과: 급성 심근경색 환자 192명중 추적 기간 내 17명의 환자에서 심장사와 재경색이 있었으며, 23명의 환자에서 불안정협심증이 있었다. 이 환자들의 혈청 GGT 값을 심장사건이 없었던 환자의 혈청 GGT 값과 비교하였을 때 통계적으로 유의한 차이를 보였다(29.5±10.0 U/L 대 25.0±11.2 U/L p=0.024). 그러나 다변량 분석에서 혈청 GGT에 영향을 줄 수 있는 혼란변수들과 알려진 심혈관계 질환의 위험인자로 보정하였을 경우 독립적인 예후 예측인자가 되지 못했다. 결론: 심질환의 과거력이 없는 심근경색 환자의 예후 예측인자로 심근경색의 급성기에 측정한 혈청 GGT 값은 통계적으로 유의한 차이는 있으나, 독립적인 예후 예측인자가 되지 못했다. Background: Serum gamma-glutamyl transferase activity (GGT) is able to catalyse low-density lipoprotein oxidation in coronary atherosclerotic plaques and has a role in the pathogenesis of atherosclerosis. GGT has been shown to be an independent risk factor for cardiac mortality in patients with a previous myocardial infarction. The purpose of this study is to determine the prognostic value of GGT within its normal range at an acute stage in patients with acute myocardial infarction. Methods: In a retrospective study, GGT and other cardiac risk factors were evaluated in 192 patients (M/F=143/49; mean age: 60.8±11.8 years) who were diagnosed with an acute myocardial infarction at the emergency room. We compared the serum GGT values for each patient with or without a cardiac event, including cardiac death, non-fetal myocardial infarction and unstable angina, after an acute myocardial infarction for a mean follow-up of 16.5±10.8 months. Results: During the follow-up period, 17 patients underwent cardiac death and experienced an acute myocardial infarction and 23 patients had unstable angina. Although the mean GGT values were significantly different from patients with cardiac events (29.5±10.0 U/L vs 25.0±11.2 U/L, p=0.024), serum GGT was not an independent cardiac risk factor for a cardiac event based on multivariate analysis adjusted for age, sex, alcohol and known cardiovascular risk factors. Conclusions: Serum GGT within its normal range at an acute stage in patients that experienced an acute myocardial infarction is not an independent prognostic marker. (Korean J Med 72:281-289, 2007)

Highly luminescent silica-coated CdS/CdSe/CdS nanoparticles with strong chemical robustness and excellent thermal stability

Wang, Nianfang,Koh, Sungjun,Jeong, Byeong Guk,Lee, Dongkyu,Kim, Whi Dong,Park, Kyoungwon,Nam, Min Ki,Lee, Kangha,Kim, Yewon,Lee, Baek-Hee,Lee, Kangtaek,Bae, Wan Ki,Lee, Doh C IOP 2017 Nanotechnology Vol.28 No.18

<P>We present facile synthesis of bright CdS/CdSe/CdS@SiO<SUB>2</SUB> nanoparticles with 72% of quantum yields (QYs) retaining ca 80% of the original QYs. The main innovative point is the utilization of the highly luminescent CdS/CdSe/CdS seed/spherical quantum well/shell (SQW) as silica coating seeds. The significance of inorganic semiconductor shell passivation and structure design of quantum dots (QDs) for obtaining bright QD@SiO<SUB>2</SUB> is demonstrated by applying silica encapsulation via reverse microemulsion method to three kinds of QDs with different structure: CdSe core and 2 nm CdS shell (CdSe/CdS-thin); CdSe core and 6 nm CdS shell (CdSe/CdS-thick); and CdS core, CdSe intermediate shell and 5 nm CdS outer shell (CdS/CdSe/CdS-SQW). Silica encapsulation inevitably results in lower photoluminescence quantum yield (PL QY) than pristine QDs due to formation of surface defects. However, the retaining ratio of pristine QY is different in the three silica coated samples; for example, CdSe/CdS-thin/SiO<SUB>2</SUB> shows the lowest retaining ratio (36%) while the retaining ratio of pristine PL QY in CdSe/CdS-thick/SiO<SUB>2</SUB> and SQW/SiO<SUB>2</SUB> is over 80% and SQW/SiO<SUB>2</SUB> shows the highest resulting PL QY. Thick outermost CdS shell isolates the excitons from the defects at surface, making PL QY relatively insensitive to silica encapsulation. The bright SiO<SUB>2</SUB>-coated SQW sample shows robustness against harsh conditions, such as acid etching and thermal annealing. The high luminescence and long-term stability highlights the potential of using the SQW/SiO<SUB>2</SUB> nanoparticles in bio-labeling or display applications.</P>

Biological and Antibacterial Activities of the Natural Herb <i>Houttuynia cordata</i> Water Extract against the Intracellular Bacterial Pathogen <i>Salmonella</i> within the RAW 264.7 Macrophage

Kim, Gon Sup,Kim, Dong Hyeok,Lim, Jeong Ju,Lee, Jin Ju,Han, Dae Yong,Lee, Whi Min,Jung, Won Chul,Min, Won Gi,Won, Chung Gil,Rhee, Man Hee,Lee, Hu Jang,Kim, Suk Pharmaceutical Society of Japan 2008 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.31 No.11

<P>Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans. Recently, the emergence of multidrug-resistant strains of <I>Salmonella</I> sp. has caused more serious problems in public health. The present study investigated the antibacterial effects of <I>Houttuynia cordata</I> water extract (HCWE) against murine salmonellosis. In RAW 264.7 cells, there was no detectable cytotoxic effect of HCWE at any concentration between 25 and 100 μg/ml after 8-h incubation. The antibacterial activity of HCWE was then examined in a <I>Salmonella enterica</I> serovar (<I>Salmonella typhimurium</I>), and was found to increase in a dose-dependent manner at concentrations from 25 to 100 μg/ml during 8-h incubation. HCWE also affected RAW 264.7 cells including morphologic change and bacterial uptake, but there was no significant difference in bacterial replication in RAW 264.7 cells. With HCWE alone, nitric oxide (NO) production by RAW 264.7 cells did not increase, but when RAW 264.7 cells were infected by <I>S. typhimurium</I>, with or without HCWE, NO production with HCWE was 2-fold higher than that without HCWE. Treatment with HCWE did not affect inducible NO synthase (iNOS) mRNA expression by RAW 264.7 cells, but when RAW 264.7 cells with HCWE were infected by <I>S. typhimurium</I>, iNOS mRNA expression was increased during 8-h incubation. Furthermore, HCWE showed virulence reduction effects in <I>S. typhimurium</I>-infected BALB/c mice. After a lethal dose of <I>S. typhimurium</I>, the mortality rate in the HCWE untreated group was 100% at 7 d, but the HCWE 25, 50, and 100 μg/ml groups survived until 11, 17, and 23 d, respectively. These data suggest that HCWE is stable and beneficial in the treatment of bacterial infection including intracellularly replicating pathogens and may solve antimicrobial misuse and overuse.</P>