Proteomic and Morphologic Evidence for Taurine-5-Bromosalicylaldehyde Schiff Base as an Efficient Anti-Mycobacterial Drug

( Wenyong Ding ),( Houli Zhang ),( Yuefei Xu ),( Li Ma ),( Wenli Zhang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.8

Mycobacterium tuberculosis, a causative pathogen of tuberculosis (TB), still threatens human health worldwide. To find a novel drug to eradicate this pathogen, we tested taurine-5- bromosalicylaldehyde Schiff base (TBSSB) as an innovative anti-mycobacterial drug using Mycobacterium smegmatis as a surrogate model for M. tuberculosis. We investigated the antimicrobial activity of TBSSB against M. smegmatis by plotting growth curves, examined the effect of TBSSB on biofilm formation, observed morphological changes by scanning electron microscopy and transmission electron microscopy, and detected differentially expressed proteins using two-dimensional gel electrophoresis coupled with mass spectrometry. TBSSB inhibited mycobacterial growth and biofilm formation, altered cell ultrastructure and intracellular content, and inhibited cell division. Furthermore, M. smegmatis adapted itself to TBSSB inhibition by regulating the metabolic pathways and enzymatic activities of the identified proteins. NDMA-dependent methanol dehydrogenase, NAD(P)H nitroreductase, and amidohydrolase AmiB1 appear to be pivotal factors to regulate the M. smegmatis survival under TBSSB. Our dataset reinforced the idea that Schiff base-taurine compounds have the potential to be developed as novel anti-mycobacterial drugs.

Cross-platform characterization of electron tunneling in molecular self-assembled monolayers

이탁희,Wenyong Wang,J. J. Zhang,J. Su, J,F. Klemic,M. A. Ree 한국물리학회 2005 Current Applied Physics Vol.5 No.3

Electron tunneling is investigated for the alkanethiol self-assembled monolayers (SAMs) formed using three dierent devicestructures spanning from the nanometer to the micrometer scale. The measured currentvoltage characteristics for the alkanethiolSAMs can be explained by the classical metalinsulatormetal tunneling model and the tunneling current exhibits overall expo-nential trend on the molecular length. Although dierent structures give consistent results (such as decay coecient), unambiguousdetermination of the tunneling requires characterization of length and temperature dependencies..

Mechatronic Control Model of the Wind Turbine with Transmission to Split Power

Tong Zhang,Wenyong Li,Yu Du 대한전기학회 2005 International Journal of Control, Automation, and Vol.3 No.4

Geochemical characteristics of trace elements and carbon isotopes in Taiyuan Formation: a case study of Huainan Coalfield

Dun Wu,Wenyong Zhang 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.6

This paper reports systematically the redox sensitive elements (Mo, V, Cd, Cr, U and Th) and carbon and oxygen isotope data of the Late Carboniferous Taiyuan Formation limestone in the Huainan Coalfield in the southern margin of North China Platform, combined with the observation of limestone slice, and tries to explain the sedimentary environment of each layer of limestone. Through the analysis of the diagenesis of each layer of limestone, except for L7 and L12 limestone, the lithogenesis change of the remaining limestone becomes weak, which generally reflects the geochemical characteristics of the paleosol during sedimentation. There are three negative shifts of δ13C in Taiyuan Formation limestone, which may be related to the enrichment of redox sensitive elements (Cd, Cr, Mo and V) in limestone. In addition, the value of the above redox sensitive elements shows a negative drift. Combining the low V/Cr ratio and high Th/U ratio, it can be concluded that limestone of the Taiyuan Formation is likely to be deposited under an oxidizing condition.

Coupling effects of vortex-induced vibration for a square cylinder at various angles of attack

Deqian Zheng,Wenyong Ma,Xiaobin Zhang,Wei Chen,Junhao Wu 한국풍공학회 2022 Wind and Structures, An International Journal (WAS Vol.34 No.5

Vortex-induced vibration (VIV) is a significant concern when designing slender structures with square cross sections. VIV strongly depends on structural dynamics and flow states, which depend on the conditions of the approaching flow and shape of a structure. Therefore, the effects of the angle of attack on the coupling effects of VIV for a square cylinder are expected to be significant in practice. In this study, the aerodynamic forces for a fixed and elastically mounted square cylinder were measured using wind pressure tests. Aerodynamic forces on the stationary cylinder are firstly discussed by comparisons of variation of statistical aerodynamic force and wind pressure coefficient with wind angle of attack. The coupling effect between the aerodynamic forces and the motion of the oscillating square cylinder by VIV is subsequently investigated in detail at typical wind angels of attack with occurrence of three typical flow regimes, i.e., leading-edge separation, separation bubble (reattachment), and attached flow. The coupling effect are illustrated by discussing the onset of VIV, characteristics of aerodynamic forces during VIV, and interaction between motion and aerodynamic forces. The results demonstrate that flow states can be classified based on final separation points or the occurrence of reattachment. These states significantly influence coupling effects of the oscillating cylinder. Vibration enhances vortex shedding, which creates strong fluctuations in aerodynamic forces. However, differences in the lock-in range, aerodynamic force, and interaction process for angles of attack smaller and larger than the critical angle of attack revealed noteworthy characteristics in the VIV of a square cylinder.

Numerical investigation on pressure responsiveness properties of the skirt-cushion system of an air cushion vehicle

Xu, Shengjie,Tang, Yujia,Chen, Kejie,Zhang, Zongke,Ma, Tao,Tang, Wenyong The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.-

The pressure responsiveness property of a skirt-cushion system, which is closely related to the overall performance of Air Cushion Vehicles (ACVs), has always been the difficulty and challenging problem involving cushion aerodynamics and flexible skirt dynamics. Based on a widely used bag and finger skirt-cushion system, the pressure responsiveness properties are investigated numerically. The physical process and mechanism are analyzed and a numerical method for evaluating the pressure responsiveness property is proposed. A cushion-skirt information communication platform is also presented for interchanging the force and the skirt configuration between cushion aerodynamics and flexible skirt dynamics. The pressure responsiveness of a typical skirt-cushion system is calculated and the results demonstrate that the pressure responsiveness property helps alleviate the influence of the cushion height changing on the overall performance of ACVs. Finally, the influences of skirt geometrical and cushion aerodynamic parameters on the pressure responsiveness properties are discussed systematically, giving insight into the design of skirt-cushion systems.

Numerical investigation on pressure responsiveness properties of the skirt-cushion system of an air cushion vehicle

Xu, Shengjie,Tang, Yujia,Chen, Kejie,Zhang, Zongke,Ma, Tao,Tang, Wenyong The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.1

The pressure responsiveness property of a skirt-cushion system, which is closely related to the overall performance of Air Cushion Vehicles (ACVs), has always been the difficulty and challenging problem involving cushion aerodynamics and flexible skirt dynamics. Based on a widely used bag and finger skirt-cushion system, the pressure responsiveness properties are investigated numerically. The physical process and mechanism are analyzed and a numerical method for evaluating the pressure responsiveness property is proposed. A cushion-skirt information communication platform is also presented for interchanging the force and the skirt configuration between cushion aerodynamics and flexible skirt dynamics. The pressure responsiveness of a typical skirt-cushion system is calculated and the results demonstrate that the pressure responsiveness property helps alleviate the influence of the cushion height changing on the overall performance of ACVs. Finally, the influences of skirt geometrical and cushion aerodynamic parameters on the pressure responsiveness properties are discussed systematically, giving insight into the design of skirt-cushion systems.