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Research of the Mechanism of Low Frequency Oscillation Based on Dynamic Damping Effect
Wenying Liu,Rundong Ge,Dandan Zhu,Weizhou Wang,Wei Zheng,Fuchao Liu 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.4
For now, there are some low frequency oscillations in the power system which feature low frequency oscillation with positive damping and cannot be explained by traditional low frequency oscillation mechanisms. Concerning this issue, the dynamic damping effect is put forward on the basis of the power-angle curve and the study of damping torque in this article. That is, in the process of oscillation, damping will dynamically change and will be less than that of the stable operating point especially when the angle of the stable operating point and the oscillation amplitude are large. In a situation with weak damping, the damping may turn negative when the oscillation amplitude increases to a certain extent, which may result in an amplitude-increasing oscillation. Finally, the simulation of the two-machine two-area system verifies the arguments in this paper which may provide new ideas for the analysis and control of some unclear low frequency phenomena.
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Research of the Mechanism of Low Frequency Oscillation Based on Dynamic Damping Effect
Liu, Wenying,Ge, Rundong,Zhu, Dandan,Wang, Weizhou,Zheng, Wei,Liu, Fuchao The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.4
For now, there are some low frequency oscillations in the power system which feature low frequency oscillation with positive damping and cannot be explained by traditional low frequency oscillation mechanisms. Concerning this issue, the dynamic damping effect is put forward on the basis of the power-angle curve and the study of damping torque in this article. That is, in the process of oscillation, damping will dynamically change and will be less than that of the stable operating point especially when the angle of the stable operating point and the oscillation amplitude are large. In a situation with weak damping, the damping may turn negative when the oscillation amplitude increases to a certain extent, which may result in an amplitude-increasing oscillation. Finally, the simulation of the two-machine two-area system verifies the arguments in this paper which may provide new ideas for the analysis and control of some unclear low frequency phenomena.
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Fine Mapping of Pa-6 Gene for Purple Apiculus in Rice
Xu Liu,Xu Sun,Wenying Wang,Hanfeng Ding,Wei Liu,Guangxian Li,Mingsong Jiang,Changxiang Zhu,Fangyin Yao 한국식물학회 2012 Journal of Plant Biology Vol.55 No.3
Purple apiculus is one of the important agronomic traits of rice. Single-segment substitution line (SSSL) W23-07-6-02-14 in the genetic background of an elite rice variety Huajingxian74 (HJX74) with the substituted interval of RM225-RM217-RM253 on the chromosome 6 was found to have purple apiculus (Pa). To map the gene governing Pa,W23-07-6-02-14 was crossed with the recipient HJX74 to develop an F2 secondary segregation population. The ratio of purple apiculus to green apiculus showed a good fit to 3:1 ratio,indicating that Pa was controlled by a major dominant gene. The gene locus for Pa was tentatively designated as Pa-6. Using 430 individuals from the F2 segregation population, the Pa-6 locus was mapped between two SSR markers RM19556and RM19561 with genetic distances of 0.2 and 0.3 cM,respectively. For fine mapping of the Pa-6 gene, a large F2:3segregation population of 3890 individuals was developed from F2 heterzygous plants in the RM19556-RM19561 region. Recombinant analyses further mapped the Pa-6 gene locus to an interval of 41.7-kb bounded L02 and RM19561. Sequence analysis of this 41.7-kb region revealed that it contains eleven open reading frames (ORFs), of which, ORF5 is classified as the one that is associated with the C (chromogen for anthocyanin) gene, it was presumed to be the candidate gene for Pa. This result provided a foundation of map-based cloning and function analysis of the Pa-6 gene.
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Chengguo Pei,Xu Liu,Wenying Wang,Hanfeng Ding,Mingsong Jiang,Guangxian Li,ChangXiang Zhu,FuJiang Wen,Fangyin Yao 한국식물학회 2011 Journal of Plant Biology Vol.54 No.3
Heading date is one of the importance agronomic traits. A library consisting of 1,123 single segment substitution lines (SSSLs) in the same genetic background of an elite rice variety Huajingxian 74 (HJX74) was evaluated for heading date (HD). From this library, the SSSL W06-26-35-1-5-2 with the substituted interval of PSM152–PSM154–PSM155–RM25–RM547–RM72–RM404 was found having a gene, which performed stable and late heading in the different environments of Shandong and Hainan provinces. To map the gene governing heading date, the SSSL W06-26-35-1-5-2 was crossed with the recipient HJX74 to develop an F_2 segregating population. The distribution of late and early heading plants in this population fitted a segregation ratio of 3:1, indicating the late heading was controlled by a dominant gene. The gene locus for heading date was tentatively designated as qHD8-1. Using a random sample of 460 individuals from the F_2population, the qHD8-1 was narrowed down to a region flanking by two SSR markers PSM155 and RM547. For fine mapping of qHD8-1, a large F_2:3 segregating population of 3,000 individuals were developed from F_2 plants heterozygous in the PSM155–RM547 region. Recombinants analysis further mapped qHD8-1 to an interval of region 26 kb with markers RM22492 and P23 bounded on the left and right sides, respectively. Sequence analysis of this 26-kb fragment revealed that it contains five putative open reading frames,which were regarded as candidates of qHD8-1. These results will be useful in cloning of the qHD8-1 gene
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Deng Jianyu,Shen Zhijie,Wang Fumin,Liu Tao,Hong Wenying,Fang Minghua,Wo Linfeng,Chu Sijie 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1
Grapholita molesta (Busck) (the oriental fruit moth; OFM) and Conogethes punctiferalis (Guenée) are both fruit and stem borers with overlapping habitats, occurrences, and outbreak cycles. These two species are in different families and they have completely different sex pheromone components. Here, the effects of the sex pheromone components of C. punctiferalis, (E)-10-hexadecenal (E10-16:Ald) and (Z)-10-hexadecenal (Z10-16:Ald) and their mixture, were evaluated on the sexual communication of OFM by examining electrophysiological (EAG) and behavioral responses. We found that a considerably large amount of E10-16:Ald or Z10-16:Ald and a tiny amount of OFM pheromone elicited comparable EAG responses in OFM males, indicating the low antennal olfactory sensitivity of OFM males to the heterospecific pheromone compounds. In two different peach orchard parcels, captures of OFM by their pheromone lures baited in delta traps were increased by at least 1.5 times when OFM pheromone lures were combined with lures that contained 1000 μg of either E10-16:Ald, Z10-16:Ald or their mixture. In two other pear orchard parcels, both E10-16:Ald and Z10-16:Ald increased captures of OFM in Unitraps in a dose-dependent manner with more than a four-fold increase. Therefore, the enhanced attractiveness of OFM lures by the two interspecific pheromones suggests their potential application to improve mass trapping, population monitoring and mating disruption of OFM.
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Haihua Yuan,Bin Jiang,Zhong-Zheng Zhu,Yachao Lu,Feng Liu,Wenying Zhang,Gang Huang,Guanshan Zhu 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.1
Purpose: Circulating free DNA (cfDNA) in plasma is promising to be a surrogate for tumor tissue DNA. However, not all epidermal growth factor receptor (EGFR) mutations in tumor tissue DNA has been detected in matched cfDNA, at least partly due to inefficient cfDNA extraction method. The purpose of this study was to establish an efficient plasma cfDNA extraction protocol. Materials and Methods: The yield of plasma cfDNA extracted by our modified phenol-chloroform (MPC) method from non-small-cell lung cancer (NSCLC) patients was compared with that by QIAamp MinElute Virus Spin kit (Qiagen kit) as control, using the Wilcoxon rank-sum test. TaqMan quantitative polymerase chain reaction (qPCR) assays were used to quantify the plasma cfDNA extracted. Both Mutant-enriched PCR (ME-PCR) coupled sequencing and DxS EGFR mutation test kit were used to evaluate the impact of extraction method on EGFR mutation analysis. Results: MPC method extracted more plasma cfDNA than Qiagen kit method (p=0.011). The proportion of longer fragment (≥202 bp) in cfDNA extracted by MPC method was significantly higher than by Qiagen kit method (p=0.002). In the sequencing maps of ME-PCR products, a higher mutant peak was observed on plasma cfDNA extracted by MPC method than by Qiagen kit method. In DxS EGFR mutation test kit results, plasma cfDNA extracted by MPC method contained more tumor-origin DNA than by Qiagen kit method. Conclusion: An improved plasma cfDNA extraction method of MPC is provided, which will be beneficial for EGFR mutation analysis for patients with NSCLC.
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Performances of Concrete Columns with Modular UHPC Permanent Formworks Under Axial Load
Yibo Yang,Baixi Chen,Yong Chen,Huanyang Zhou,Fucai Liu,Xiangming Xie,Junsheng Chen,Wenying Guo,Hengchang Wang 한국콘크리트학회 2023 International Journal of Concrete Structures and M Vol.17 No.5
This research proposed the modular prefabricated permanent formwork system made of ultra-high-performance concrete (UHPC). Two kinds of modular formwork shapes were designed: the flat formwork and the ribbed. The experimental investigation on the axial compression performance of the composite columns that consist of the normal strength concrete (NSC) core and the modular UHPC permanent formwork was demonstrated. Compared with the flat formwork, the ribbed formwork exhibited better bonding with the NSC core. As observed from the test results, the composite column with the ribbed formwork presented a similar axial behavior as the NSC column with a slight improvement in ultimate loads. Therefore, the modular UHPC ribbed permanent formwork could be regarded as the additional cover to the conventional NSC column. In addition, the finite element analysis (FEA) model was also developed to simulate the composite columns numerically. The predicted capacities agreed with the experimental results, which validated the numerical models. The crack pattern estimated by the FEA model revealed that the interaction between the permanent formwork and the inner concrete introduced many tiny cracks to the concrete core. However, as protected by the UHPC permanent formwork, the overall durability of the composite columns can still be enhanced.
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Autophagy is a major regulator of beta cell insulin homeostasis
Riahi, Yael,Wikstrom, Jakob D.,Bachar-Wikstrom, Etty,Polin, Nava,Zucker, Hava,Lee, Myung-Shik,Quan, Wenying,Haataja, Leena,Liu, Ming,Arvan, Peter,Cerasi, Erol,Leibowitz, Gil Springer-Verlag 2016 Diabetologia Vol.59 No.7
<P>Aims/hypothesis We studied the role of protein degradation pathways in the regulation of insulin production and secretion and hypothesised that autophagy regulates proinsulin degradation, thereby modulating beta cell function. Methods Proinsulin localisation in autophagosomes was demonstrated by confocal and electron microscopy. Autophagy was inhibited by knockdown of autophagy-related (ATG) proteins and using the H+-ATPase inhibitor bafilomycin-A1. Proinsulin and insulin content and secretion were assessed in static incubations by ELISA and RIA. Results Confocal and electron microscopy showed proinsulin localised in autophagosomes and lysosomes. Beta-Atg7(-/-) mice had proinsulin-containing sequestosome 1 (p62 [also known as SQSTM1])(+) aggregates in beta cells, indicating proinsulin is regulated by autophagy in vivo. Short-term bafilomycin-A1 treatment and ATG5/7 knockdown increased steady-state proinsulin and hormone precursor chromogranin A content. ATG5/7 knockdown also increased glucose- and non-fuel-stimulated insulin secretion. Finally, mutated forms of proinsulin that are irreparably misfolded and trapped in the endoplasmic reticulum are more resistant to degradation by autophagy. Conclusions/interpretation In the beta cell, transport-competent secretory peptide precursors, including proinsulin, are regulated by autophagy, whereas efficient clearance of transport-incompetent mutated forms of proinsulin by alternative degradative pathways may be necessary to avoid beta cell proteotoxicity. Reduction of autophagic degradation of proinsulin increases its residency in the secretory pathway, followed by enhanced secretion in response to stimuli.</P>
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