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- 저자 : Weizhou Wang (검색결과 5 건)
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Wang, Zhi,Xu, Weizhou,Kang, Jiyue,Li, Min,Huang, Jin,Ke, Qingbo,Kim, Ho Soo,Xu, Bingcheng,Kwak, Sang-Soo Elsevier 2018 Vol. No.
<P><B>Abstract</B></P> <P>The multifunctional Orange (Or) protein plays crucial roles in carotenoid homeostasis, photosynthesis stabilization, and antioxidant activity in plants under various abiotic stress conditions. The <I>Or</I> gene has been cloned in several crops but not in alfalfa (<I>Medicago sativa</I> L.). Alfalfa is widely cultivated across the world; however, its cultivation is largely limited by various abiotic stresses, including drought. In this study, we isolated the <I>Or</I> gene from alfalfa (<I>MsOr</I>) cv. Xinjiang Daye. The amino acid sequence of the deduced MsOr protein revealed that the protein contained two trans-membrane domains and a DnaJ cysteine-rich zinc finger domain, and showed a high level of similarity with the Or protein of other plants species. The MsOr protein was localized in leaf chloroplasts of tobacco. The expression of <I>MsOr</I> was the highest in mature leaves and was significantly induced by abiotic stresses, especially drought. To perform functional analysis of the <I>MsOr</I> gene, we overexpressed <I>MsOr</I> gene in tobacco (<I>Nicotiana benthamiana</I>). Compared with wild-type (WT) plants, transgenic tobacco lines showed higher carotenoid accumulation and increased tolerance to various abiotic stresses, including drought, heat, salt, and methyl viologen-mediated oxidative stress. Additionally, contents of hydrogen peroxide and malondialdehyde were lower in the transgenic lines than in WT plants, suggesting superior membrane stability and antioxidant capacity of TOR lines under multiple abiotic stresses. These results indicate the <I>MsOr</I> gene as a potential target for the development of alfalfa cultivars with enhanced carotenoid content and tolerance to multiple environmental stresses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Orange (<I>MsOr</I>) gene from alfalfa was isolated and characterized in transgenic tobacco. </LI> <LI> <I>MsOr</I> gene was localized to chloroplasts and strongly induced by abiotic stresses including drought. </LI> <LI> <I>MsOr</I> expressing tobacco plants showed enhanced tolerance to drought, heat, salt and oxidative stress. </LI> </UL> </P>
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Research of the Mechanism of Low Frequency Oscillation Based on Dynamic Damping Effect
Liu, Wenying,Ge, Rundong,Zhu, Dandan,Wang, Weizhou,Zheng, Wei,Liu, Fuchao The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.4
For now, there are some low frequency oscillations in the power system which feature low frequency oscillation with positive damping and cannot be explained by traditional low frequency oscillation mechanisms. Concerning this issue, the dynamic damping effect is put forward on the basis of the power-angle curve and the study of damping torque in this article. That is, in the process of oscillation, damping will dynamically change and will be less than that of the stable operating point especially when the angle of the stable operating point and the oscillation amplitude are large. In a situation with weak damping, the damping may turn negative when the oscillation amplitude increases to a certain extent, which may result in an amplitude-increasing oscillation. Finally, the simulation of the two-machine two-area system verifies the arguments in this paper which may provide new ideas for the analysis and control of some unclear low frequency phenomena.
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Research of the Mechanism of Low Frequency Oscillation Based on Dynamic Damping Effect
Wenying Liu,Rundong Ge,Dandan Zhu,Weizhou Wang,Wei Zheng,Fuchao Liu 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.4
For now, there are some low frequency oscillations in the power system which feature low frequency oscillation with positive damping and cannot be explained by traditional low frequency oscillation mechanisms. Concerning this issue, the dynamic damping effect is put forward on the basis of the power-angle curve and the study of damping torque in this article. That is, in the process of oscillation, damping will dynamically change and will be less than that of the stable operating point especially when the angle of the stable operating point and the oscillation amplitude are large. In a situation with weak damping, the damping may turn negative when the oscillation amplitude increases to a certain extent, which may result in an amplitude-increasing oscillation. Finally, the simulation of the two-machine two-area system verifies the arguments in this paper which may provide new ideas for the analysis and control of some unclear low frequency phenomena.
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LAMP, PCR, and real-time PCR detection of Acetobacter aceti in yogurt
Wei Zhou,Yan Zhang,Shuang Wang,Yuehua Li,Jingjing Zhang,Cuixia Zhang,Zan Wang,Zhisheng Zhang 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.1
Acetic acid bacteria (AAB) can spoil food. Acetobacter aceti as a core subgroup of AAB is usually isolated from yogurt. A. aceti should be timely and effectively detected to prevent yogurt contamination. The present study focused on A. aceti to establish an assay that can be performed to detect AAB in yogurt. LAMP, PCR, and real-time PCR were applied and compared for detecting A. aceti from pure culture and artificially contaminated yogurt samples. In pure culture, LAMP showed the highest detection sensitivity with 10−1 CFU/mL. For yogurt samples, the sensitivity limit of LAMP was 102 CFU/mL, which was lower than that of real-time PCR (101 CFU/mL). The results indicated that these methods could be quickly and efficiently applied to detect A. aceti. As LAMP technology has low cost and high detection efficiency, it can potentially be applied for detecting A. aceti in production and quality control programs of yogurt.
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Wei Zhou,Zhi-yuan Wei,Guang-fei Wang,Kang-ning Han,Ran Liu,Lian-Hua Ma 한국섬유공학회 2021 Fibers and polymers Vol.22 No.4
Accurate characterisation of transverse tensile deformation and damage evolution is of importance for evaluatingthe failure behaviors of three-dimensional (3D) braided composites. In the present study, a finite element method (FEM) andseveral non-destructive testing methods including acoustic emission, digital image correlation, and infrared thermography aredeveloped to investigate the transverse tensile deformation and damage evolution of 3D five-directional braided composites. In the finite element approach, a matrix-impregnated fiber bundles (MIFB) model and a representative volume cell (RVC)model, which take into account the fiber bundles and matrix, are respectively established to predict the effective mechanicalproperties of fiber bundles and simulate the deformation and progressive damage of such composites. The damaged locationsand the failure modes including matrix crack, fiber debonding and shear fracture of fiber are predicted and verified byexperimental tests. The non-destructive tests show that the transverse tensile fracture process can be divided into four stageswhich correspond to acoustic emission signals severally. The combination of the FEM based numerical modeling andmultiple non-destructive characterisation methods can accurately monitor the deformation and damage behaviors of 3Dbraided composites under transverse tensile loads and thus provide a reference for structural health monitoring of compositesin practical application.
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