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Wenxin Li,반규정,Da-Hao Zheng,Weixian Liu,이영호,Sue Yeon Lee,이준호,Suk-Ha Lee 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.4
The bean bug Riptortus clavatus Thunberg (Heteroptera: Alydidae) is an important pest, causing serious yield loss in soybean. Butthe information on mechanism of resistance to R. clavatus is limited. The objective of this study was to identify QTLs for R. clavatusresistance using simple sequence repeat (SSR) markers in a soybean population of recombinant inbred lines (RILs) developed fromthe cross PI 171451 × Hwaeomputkong. A genetic map from this population was constructed with a total of 136 SSR markers covering1073.9 cM on 20 linkage groups (LGs). With 126 F5 RILs, two independent QTLs for resistance to R. clavatus were mapped onLGs B1 and C2. The amount of phenotypic variation explained by these QTLs ranged from 12 to 16%. PI 171451 showed an escaperesponse to R. clavatus. Under feeding conditions, 14.4% of RILs showed greater resistance to R. clavatus than the resistant parent. The resistance to R. clavatus in soybean from PI 171451 was incomplete and quantitatively inherited and the QTLs for resistance toR. clavatus detected in the RIL population were not significantly affected by epistatic interactions.
Impact of Grafting Density on the Self-Assembly and Hydrophilicity of Succinylated Collagen
Wenxin Wang,Feiyi Shu,Lingyu Pan,Shanshan Huang,Xiao Tu,Ping Li,Siman Li,Yuanyuan Li,Chengzhi Xu,Yang Sun,Juntao Zhang,Haibo Wang 한국고분자학회 2020 Macromolecular Research Vol.28 No.6
Modification of protein could give their new functionality but would affect their intrinsic property and activity. In this paper, a series of succinylated collagen (SCol(n/1)) (n≥1) were prepared by modification of collagen with succinic anhydride at different molar ratio to amino groups amount of collagen. The impact of grafting density on the intrinsic self-assembly and additional hydrophilicity of succinylated collagen was explored. The results revealed that excessive grafting density of succinylated collagen would improve their hydrophilicity but weaken their self-assembly property, although the triple helix of collagen could be reserved after succinylation. SCol(1/1) (grafting density of 17%) with self-assembly property and good hydrophilicity was chosen to compare with native collagen. Compared to native collagen, thermostability of SCol(1/1) decreased slightly, however, SCol(1/1) could form softer hydrogel, which was more favorable for the proliferation of NIH/3 T3. The present work would help us to further understand the importance of grafting density for the design of modified collagen with intrinsic self-assembly property and additional new functionality.
Li, Wenxin,Van, Kyujung,Zheng, Da-Hao,Liu, Weixian,Lee, Yeong-Ho,Lee, Sue-Yeon,Lee, Joon-Ho,Lee, Suk-Ha The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.4
The bean bug Riptortus clavatus Thunberg (Heteroptera: Alydidae) is an important pest, causing serious yield loss in soybean. But the information on mechanism of resistance to R. clavatus is limited. The objective of this study was to identify QTLs for R. clavatus resistance using simple sequence repeat (SSR) markers in a soybean population of recombinant inbred lines (RILs) developed from the cross PI 171451 ${\times}$ Hwaeomputkong. A genetic map from this population was constructed with a total of 136 SSR markers covering 1073.9 cM on 20 linkage groups (LGs). With 126 $F_5$ RILs, two independent QTLs for resistance to R. clavatus were mapped on LGs B1 and C2. The amount of phenotypic variation explained by these QTLs ranged from 12 to 16%. PI 171451 showed an escape response to R. clavatus. Under feeding conditions, 14.4% of RILs showed greater resistance to R. clavatus than the resistant parent. The resistance to R. clavatus in soybean from PI 171451 was incomplete and quantitatively inherited and the QTLs for resistance to R. clavatus detected in the RIL population were not significantly affected by epistatic interactions.
Li Yansong,Jiang Tao,Du Mengyu,He Shuxuan,Ning Huang,Cheng Bo,Yan Chaoying,Tang Wenxin,Gao Wei,Guo Hongyan,Li Qiao,Wang Qiang 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Dementia, as an advanced diabetes-associated cognitive dysfunction (DACD), has become the second leading cause of death among diabetes patients. Given that little guidance is currently available to address the DACD process, it is imperative to understand the underlying mechanisms and screen out specific therapeutic targets. The excessive endogenous fructose produced under high glucose conditions can lead to metabolic syndrome and peripheral organ damage. Although generated by the brain, the role of endogenous fructose in the exacerbation of cognitive dysfunction is still unclear. Here, we performed a comprehensive study on leptin receptor-deficient T2DM mice and their littermate m/m mice and revealed that 24-week-old db/db mice had cognitive dysfunction and excessive endogenous fructose metabolism in the hippocampus by multiomics analysis and further experimental validation. We found that the rate-limiting enzyme of fructose metabolism, ketohexokinase, is primarily localized in microglia. It is upregulated in the hippocampus of db/db mice, which enhances mitochondrial damage and reactive oxygen species production by promoting nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) expression and mitochondrial translocation. Inhibiting fructose metabolism via ketohexokinase depletion reduces microglial activation, leading to the restoration of mitochondrial homeostasis, recovery of structural synaptic plasticity, improvement of CA1 pyramidal neuron electrophysiology and alleviation of cognitive dysfunction. Our findings demonstrated that enhanced endogenous fructose metabolism in microglia plays a dominant role in diabetes-associated cognitive dysfunction and could become a potential target for DACD.
QTL Mapping for Major Agronomic Traits across Two Years in Soybean(Glycine max L. Merr.)
Li, Wenxin,Zheng, Da-Hao,Van, Kyu-Jung,Lee, Suk-Ha The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.3
The agronomic traits, such as days to flowering and maturity, plant height, 100-seed weight and seed filling period, are quantitatively inherited and important characters in soybean(Glycine max L. Merr.). A total of 126 $F_5$ recombinant inbred lines(RILs) developed from the cross of PI 171451$\times$Hwaeomputkong were used to identify quantitative trait loci(QTLs) for days to flowering(FD), days to maturity(MD), plant height(PH), 100-seed weight(SW), number of branches(NB) and seed filling period(FP). A total of 136 simple sequence repeat(SSR) markers segregated in a RIL population were distributed over 20 linkage groups(LGs), covering 1073.9 cM of the soybean genome with the average distance between adjacent markers of 7.9 cM. Five independent QTLs were identified for FD, three for MD, two for PH, three for SW, one for NB and one for FP. Of these, three QTLs were related to more than two traits of FD, MD, PH, NB and FP and mapped near the same positions on LGs H and O. Thus, these traits could be correlated with biologically controlled major QTLs in this soybean RIL population.
QTL Mapping for Major Agronomic Traits across Two Years in Soybean (Glycine max L. Merr.)
Wenxin Li,Da-Hao Zheng,반규정,Suk-Ha Lee 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.3
The agronomic traits, such as days to flowering and maturity, plant height, 100-seed weight and seed filling period, are quantitativelyinherited and important characters in soybean (Glycine max L. Merr.). A total of 126 F5 recombinant inbred lines (RILs) developed fromthe cross of PI 171451×Hwaeomputkong were used to identify quantitative trait loci (QTLs) for days to flowering (FD), days to maturity(MD), plant height (PH), 100-seed weight (SW), number of branches (NB) and seed filling period (FP). A total of 136 simple sequencerepeat (SSR) markers segregated in a RIL population were distributed over 20 linkage groups (LGs), covering 1073.9 cM of the soybeangenome with the average distance between adjacent markers of 7.9 cM. Five independent QTLs were identified for FD, three for MD, twofor PH, three for SW, one for NB and one for FP. Of these, three QTLs were related to more than two traits of FD, MD, PH, NB and FPand mapped near the same positions on LGs H and O. Thus, these traits could be correlated with biologically controlled major QTLs inthis soybean RIL population.
Design and optimization of novel transceiver device for one‑way single‑wire power transfer
Yang Li,Taocheng Hu,Xueliang Wang,Yujie Zhai,Yao Li,Wenxin Huang,Zhigang Lou 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.10
The further development of wireless power transfer technology is hindered by the limiting relationship between transmission distance, transmission efficiency, and size of the coupling mechanism. Thus, a feasible solution was provided by one-way single-wire power transfer. First, a horn-type mode conversion device was proposed in this study by analyzing the principle of one-way single-wire power transfer. Second, the system structure was designed to save materials and reduce the volume of space. The transmission efficiency of the system was improved, and the operating frequency was reduced by optimizing the parameters of the mode conversion device. Finally, the feasibility and structural applicability of the one-way single-wire power transfer method proposed in this study were proved by the experimental results.
Cooperation between Human DAF and CD59 in Protecting Cells from Human Complement-mediated Lysis
Xu, Li,Wu, Wenlan,Zhao, Zhouzhou,Shao, Huanjie,Liu, Wanhong,Liu, Hui,Li, Wenxin Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6
The complement (C) regulatory proteins decay accelerating factor (DAF, CD55) and CD59 could protect host cells using different mechanisms from C-mediated damage at two distinct levels within the C pathway. Co-expression of DAF and CD59 would be an effective strategy to help overcome host C-induced xenograft hyperacute rejection. In this study, we made a construct of recombinant expression vector containing DAF and CD59 cDNA and the stable cell lines were obtained by G418 selection. Extraneous genes integration and co-expression were identified by PCR, RT-PCR and Western blot analysis. Human c-mediated cytolysis assays showed that NIH/3T3 cells transfected stably with pcDNA3-CD59, pcDNA3-DAF, and pcDNA3-CD59DAF-DP were protected from C-mediated damage and that synchronously expressed human CD59 and DAF provided the most excellent protection for host cells as compared with either human CD59 or DAF expressed alone. Therefore, the construct represents an effective and efficacy strategy to overcome C-mediated damage in cells and, ultimately, in animals.
Decentralized Neural Network-based Excitation Control of Large-scale Power Systems
Wenxin Liu,Jagannathan Sarangapani,Ganesh K. Venayagamoorthy,Li Liu,Donald C. Wunsch II,Mariesa L. Crow,David A. Cartes 대한전기학회 2007 International Journal of Control, Automation, and Vol.5 No.5
This paper presents a neural network based decentralized excitation controller design for large-scale power systems. The proposed controller design considers not only the dynamics of generators but also the algebraic constraints of the power flow equations. The control signals are calculated using only local signals. The transient stability and the coordination of the subsystem control activities are guaranteed through rigorous stability analysis. Neural networks in the controller design are used to approximate the unknown/imprecise dynamics of the local power system and the interconnections. All signals in the closed loop system are guaranteed to be uniformly ultimately bounded. To evaluate its performance, the proposed controller design is compared with conventional controllers optimized using particle swarm optimization. Simulations with a three-machine power system under different disturbances demonstrate the effectiveness of the proposed controller design.