Influence of Light on Biomass of Soybean in Narrow Strip Cropping of Oat, Corn, and Soybean

반규정 한국작물학회 2002 Korean journal of crop science Vol.47 No.6

The strip intercropping system has used due to many advantages. Many researches supported these crop systems are usually equal to or better than monoculture crop systems in both total production and profit. There was no research to examine the important ecological factors in the competition between crops. A strip intercropping system composed of adjacent narrow strips of corn, soybean, and oat/legumes has been investigated in Iowa, USA. This study conducted to investigate why and how the differences in soybean yield are produced and affected by light, one of the microclimate, of the strip intercropping system. In height, the two rows of soybean closest to corn were taller than the two rows near the then-empty oat strip. The height of each crop decreased as the amount of light received increased. Weight of plant parts was lowest in row 1, nearest corn, and highest in row 4, next to the vacant oat strip. Daily photon flux density(PFD) increased with increasing distance from corn, with the highest value occurring on the edge next to the empty oat strip. Analyses of the relationship between light and biomass of soybean showed that all biomass measurements had a positive relationship to total PFD per day except height.

SSR Mapping of Genes Conditioning Soybean Resistance to Six Isolates of Xanthomonas axonopodis pv. Glycines

반규정,하보균,김문영,문중경,백남천,허성기,이석하 한국유전학회 2004 Genes & Genomics Vol.26 No.1

Molecular Characterization of Hypernodulation in Soybean

반규정,하보근,김문영,이석하,Eun-YoungHwang,SunggiHeu 한국식물병리학회 2003 Plant Pathology Journal Vol.19 No.1

SS2-2, a hypernodulating soybean mutant, was isolated by EMS mutagenesis from Sinpaldalkong 2. This autoregulation mutant showed greater number of nodules and smaller plant size than its wild type Sinpaldalkong 2. SSR markers were used to identify DNA variation at SSR loci from different soybean LG. The only SSR marker that detected a length polymorphism between SS2-2 and its wild type ancestor was Satt294 on LG C1 instead of LG H, locating a hypernodulating gene. Sequencing data of flanking Satt294 indicated that the size variation was due to extra stretch of TTA repeats of the SSR motif in SS2-2, along with AG transversion. In spite of phenotypic differences between the wild type and its hypernodulating mutants, genomic DNA polymorphisms at microsatellite loci could not control regulation of nodule formation. The cDNA-AFLP method was applied to compare differential display of cDNA between Sinpaldalkong 2 and SS2-2. After isolation and sequence comparison with many AFLP fragments, several interesting genes were identified. Northern blot analysis, immunolocalization and/or the yeast two-hybrid system with these genes might provide information on regulation of nodule development in SS2-2.

Genome-wide scan of the soybean genome using degenerate oligonucleotide primed PCR: an example for studying large complex genome structure

반규정,강양제,심상리,이석하 한국유전학회 2012 Genes & Genomics Vol.34 No.5

The availability of next generation sequencing instruments has made large-scale and genome-wide sequence analysis more feasible in a wild variety of species with large complex genomes,especially crops. This report suggested an approach for characterizing large complex genomes of less-studied/orphan crops. Degenerate oligonucleotide primed PCR (DOP-PCR) is a useful tool for the survey of genomes in less-studied/orphan crops, as prior sequence information is not necessary. Here, four different degenerate primers were redesigned from previously described DOP-PCR primers. The degeneracy of these primers was increased with the addition of two more ‘Ns’. The amplified DOP-PCR products from Sinpaldalkong 2, a soybean genotype, were applied to GS-FLX and the reads from Sinpaldalkong 2 were mapped against Williams 82 as a reference (http://www.phytozome.net/soybean. php), using the Burrows-Wheeler Aligner (http://bio-bwa. sourceforge.net/). These results suggest the identification of 4 single nucleotide polymorphisms between Sinpaldalkong 2 and Williams 82 and recent duplication of the soybean genome. The sequenced reads were subsequently assembled into contigs by Newbler under default conditions. A total of 29 Sinpaldalkong 2 contigs exhibited 95% similarity and <E-100 when mega-blasted with Williams 82 reference sequences. These contigs were mapped to the soybean chromosomes and positioned as clusters within each chromosome. Most of the contigs also showed similarity with the Arabidopsis RNase H domain-containing protein, suggesting a potential way to study retrotransposons in less-studied/orphan crops. Using these modified DOP-PCR primers and GS-FLX, it is possible to obtain insight into the large complex genomes of less-studied/orphan crops.

Genetic Variation in Sproout-related Traits and Microsatellite DNA Loci of Soybean

반규정,김문영,이석하,Jae-GyunGwang,Kyung-GeunBae,YoungJinOh,KyongHoKim,HoKiPark 한국작물학회 2003 Korean journal of crop science Vol.48 No.5

Genetic diversity and soybean sprout-related traits were evaluated in a total of 72 soybean accessions (60 Glycine max, 7 Glycine soja, and 5 Glycine gracilis). 100-seed weight (SW) was greatly varied and ranged from 3.2g to 32.3g in 72 soybean accessions. Positive correlation was observed between GR and hypocotyl length (HL), whereas negative correlation was observed between SW and hypocotyl diameter (HD). Re-evaluation by discarding two soybean genotypes characterized with low GR indicated that much higher correlation of sprout yield (SY) with HD and SW. Based on the principal component analysis (PCA) for sprout-related traits, 57 accessions were classified. Soybean genotypes with better traits for sprout, such as small size of seeds and high SY, were characterized with high PCA 1 and PCA 2 values. The seed size in second is small but showed low GR and SY, whereas the third has large seed, high GR and more than 400% SY. In genetic similarity analysis using 60 SSR marker genotyping, 72 accessions were classified into three major and several minor groups. Nine of twelve accessions that were identified as the representatives of soybean for sprout based on PCA were in a group by the SSR marker analysis, indicating the SSR marker selection of parental genotypes for soybean sprout improvement program.

Molecular Marker Characterization of a Supernodulating Soybean Mutant, SS2-2

반규정,김기승,하보근,전태환,장현주,김문영,이석하 한국육종학회 2005 한국육종학회지 Vol.37 No.1

Symbiotic nodulation on root in legumes is regulated not only by environmental factors but also by autoregulation. Among soybean mutants deficient autoregulation, SS2-2, supernodulating mutant, was intensively studied. SS2-2 was generated by ethylmethane sulfonate (EMS) mutagenesis like other super-/hypernodulating mutants, nts382 and nts1116. To identify DNA variation at 67 SSR loci, the simple sequence repeat (SSR) polymorphisms were detected with 46 out of 67 primer pairs among the wild types. The only polymorphic SSR marker between SS2-2 and its wild-type ancestor was Satt294 on LG C1 instead of LG H where a super-/hyperndulating gene located. Sequencing data of amplicons using primers flanking the Satt294 for Sinpaldalkong 2 and SS2-2 indicated that the size variation was due to extra stretch of ATT repeat of the SSR motif in SS2-2, along with A→G transition. After SNPs were also surveyed between Sinpaldalkong 2 and SS2-2, only one SNP was detected in the exon region.

Adaptive responses to abiotic and biotic stresses in plants

반규정 한국작물학회 2002 한국작물학회 학술발표대회 논문집 Vol.2002 No.2

Regeneration of Plants from EMS-treated Immature Embryo Cultures in Soybean [Glycine max (L.) Merr.]

반규정,장현주,이석하,장영은 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.2

Since somatic embryogenesis combined with ethylmethane sulfonate (EMS) treatments is the most efficient technique for mutagenesis, the embryogenic capacity of four soybean cultivars was evaluated at different EMS concentrations, treatment times, and preculture durations. Two to 4 mm long immature cotyledons were placed in induction medium after EMS treatment, and the numbers of somatic embryos formed per explant were counted four weeks after culture initiation. We observed genotypic differences in the efficiency of somatic embryogenesis from immature embryos among four cultivars treated with different concentrations of EMS for six hours. Cultivars, Sinpaldalkong 2 and Jack, displayed highly efficient somatic embryogenesis regardless of EMS concentration, whereas very low efficiency or no survival was observed in Jinju 1 and Iksannamulkong cultivars. Preculture duration did not influence the efficiency of somatic embryogenesis. Because Sinpaldalkong 2 exhibited the best somatic embryogenesis, much higher concentrations of EMS were used to test somatic embryo formation under different periods of time in this cultivar. Three and six hour treatments with both 1 and 2 mM EMS yielded higher embryo formation than longer periods of time. Increasing the time with embryos in 2 mM EMS caused a reduction in somatic embryogenesis in Sinpaldalkong 2, but many chlorophyll-deficient soybean variants were identified in the M₁R0 and M₂R₁ generations. In addition to Jack, Sinpaldalkong 2 is a good genotype for plant regeneration from EMS-treated immature embryo cultures. Since somatic embryogenesis combined with ethylmethane sulfonate (EMS) treatments is the most efficient technique for mutagenesis, the embryogenic capacity of four soybean cultivars was evaluated at different EMS concentrations, treatment times, and preculture durations. Two to 4 mm long immature cotyledons were placed in induction medium after EMS treatment, and the numbers of somatic embryos formed per explant were counted four weeks after culture initiation. We observed genotypic differences in the efficiency of somatic embryogenesis from immature embryos among four cultivars treated with different concentrations of EMS for six hours. Cultivars, Sinpaldalkong 2 and Jack, displayed highly efficient somatic embryogenesis regardless of EMS concentration, whereas very low efficiency or no survival was observed in Jinju 1 and Iksannamulkong cultivars. Preculture duration did not influence the efficiency of somatic embryogenesis. Because Sinpaldalkong 2 exhibited the best somatic embryogenesis, much higher concentrations of EMS were used to test somatic embryo formation under different periods of time in this cultivar. Three and six hour treatments with both 1 and 2 mM EMS yielded higher embryo formation than longer periods of time. Increasing the time with embryos in 2 mM EMS caused a reduction in somatic embryogenesis in Sinpaldalkong 2, but many chlorophyll-deficient soybean variants were identified in the M₁R0 and M₂R₁ generations. In addition to Jack, Sinpaldalkong 2 is a good genotype for plant regeneration from EMS-treated immature embryo cultures.

콩 종자 품질 향상을 위한 콩 유전체 연구

반규정 ( Kyu Jung Van ),김문영 ( Moon Young Kim ),채춘매 ( Chun Mei Cai ),전태환 ( Tae Hwan Jun ),이석하 ( Suk Ha Lee ) 한국콩연구회 2005 韓國콩硏究會誌 Vol.22 No.1

Genotypic Variation in Flowering and Maturing Periods and Their Relations with Plant Yield and Yield Components in Soybean

이석하,반규정,김문영,Ngon Trong Truong 한국작물학회 2006 Korean journal of crop science Vol.51 No.3

Improvement of crop yield can be achievedthrough understanding genetic variation in reproductivecharacters and its impact on yield components. Thepresent study was performed to evaluate genetic diversityresources and to determine the relationships betweendevelopmental and growth periods with yield and yieldcomponents in soybean cultivar groups. For phenotypicevaluation such as reproductive and agronomic traits, atotal of 80 indigenous and exotic soybean cultivars col-lected from four different geographical regions (China,Japan, Korea, and Vietnam) were grown from May toNovember of 2003 at the Seoul National University Farm,Suwon, Korea (127o02’E longitude, 37o26N latitude).Most of all the characters exhibited wide range of pheno-typic variation, of which pod number, sed number, andplant yield showed greater range as compared to othercharacters. Korean cultivar groups showed greaterdiversity than the other cultivar groups in seven charac-ters. Corelation analysis showed that days to flowering(DTF) and days to maturity (DTM) had close associationwith agronomic traits as wel as yield and yield compo-the other characters except one hundred sed weight.Stepwise multiple linear regression revealed that seed andpod number were identified as being significant for plantyield. The results in this study indicated wide variation inagronomic traits including DTF and DTM, sugesting thevaluable genetic resources in a soybean breeding program.