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Steel Bridge Construction of Hong Kong–Zhuhai–Macao Bridge
Wen-bo Gao,Quan-ke Su,Jin-wen Zhang,Hong-bing Xie,Feng Wen,Fang Li,Ji-zhu Liu 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.5
The 55-km-long Hong Kong–Zhuhai–Macao Bridge (HZMB) is the world’s longest sea-crossing bridge, connecting Hong Kong with Zhuhai and Macao at the mouth of the Pearl River Estuary in China, comprising 22.9-km-long steel bridges. HZMB is the leading steel bridge in China, with top-level manufacturing and installation technology. This paper outlines the steel bridge construction experiences of HZMB to provide comparisons for the construction of other long sea-crossing steel bridges at home or abroad. The main considerations of construction constraints, scheme selection, structural and aesthetic design of HZMB are presented, and the following points related to new strategies in the steel bridge construction of HZMB are elaborated: (1) construction quality assurance, (2) automatic manufacturing technology, (3) large segment off shore installation, (4) eco-friendly paint (content limitation of volatile organic compounds) and new multifunctional inspection gantry, and (5) Guss Mastic Asphalt steel deck pavement system. The successful implementation of those strategies shows that the steel bridge construction of HZMB promotes improvement in the overall construction and management level of the Chinese bridge industry. The advanced experience of HZMB has opened up broad prospects for the design and construction of off shore bridge engineering in China.
Wen Li,Xue-ping Li,Yuan-zhi Shao,Jiang-hui Xie,Wei-xin Chen,Zhi-qiang Jin 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6
Real-time reverse transcriptase (RT)-PCR using SYBR GreenI was applied to quantify sucrose phosphate synthase (SPS) mRNA expression at different storage days during the natural ripening (with no treatment), inhibited ripening with ethylene absorbent and accelerated ripening by treatment with propylene of banana fruit. The results showed that expression level of SPS mRNA were significantly different between 3 kinds of ripening fruit. Expression of SPS mRNA in fruit treated with propylene was dramatically promoted, the peak of SPS mRNA expression in these fruits appeared 16 days earlier than that in fruit with natural ripening. On the contrary, when fruit applied with ethylene absorbent, SPS mRNA expression was remarkably inhibited, the amount of SPS mRNA expression were significantly lower than that in naturally ripened fruit. These results indicated that the expression level of SPS mRNA of banana fruit can be impacted by regulating ethylene evolution, and that close relationships may exist between SPS mRNA expression and SPS activity, fruit softening, respiration rate, and ethylene production during ripening of banana fruit.
Protective activities of probiotics in letrozole-induced polycystic ovarian syndrome (PCOS) rats
Xinyu Li,Chenyang Xie,Qiuping Huang,Eun Mi Hwang,Bin Jin,Zhewen Dong,Zhen Zeng,Wen He,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
To investigate the effect of Bifidobacterium, C. butyricum and L. plantarum on the letrozole induced PCOS rats. All female SD rats were randomly divided into five groups, modeling PCOS rats, and respectively given intragastric administration of Bifidobacterium, C. butyricum, and L. plantarum solution (1×10<SUP>9</SUP> CFU/mL) for 30 days, and observed body weight (BW). The serum levels of testosterone (T), LH, E2, FSH, INS, TG, TC, LDL, HDL were determined by ELISA kits. The levels of TGF-β1, smad4, smad3, p-smad3, p-smad2, smad2 were detected by Western Blotting. Compared with normal group, the BW, T, LH, INS, TG, TC LDL-C in PCOS rats were significantly increased, and the levels of E2, FSH and HDL-C were significantly decreased. Administrated with different probiotics can decrease the BW, T, LH, INS, TG, TC,LDL-C, and the levels of E2, FSH and HDL-C increased in PCOS rats. All probiotics significantly decreased the expression of p-smad2, p-smad3, smad4, TGF-β1. Our results showed that Bifidobacterium, C. butyricum and L. plantarum were all effective in regulating hormone levels, improving IR and dyslipidemia, and improving ovarian fibrosis in PCOS rats.
Yong-Li Zhou,Xue-Wen Xie,Mei-Rong Xu,Jin-Ping Zang,Ling-Hua Zhu,Jian-Long Xu,Zhi-Kang Li 한국식물학회 2012 Journal of Plant Biology Vol.55 No.2
The genetic components responsible for the qualitative and quantitative resistance of rice to three Chinese races (C2, C4, and C5) of Xanthomonas oryzae pv. oryzae (Xoo)were investigated at the seedling and adult stages in two successive years in set of Lemont/Teqing cross introgression lines (ILs) in a Teqing background, to create a complete linkage map using 160 well-distributed SSR markers. Teqing was resistant to C2 and C4, but moderately susceptible to C5,whereas Lemont was susceptible to all three races. Highly significant correlations were detected among the resistance to different races at different developmental stages. A major gene (Xa4), 14 main-effect QTLs (M-QTLs), and 18 epistatic QTLs were identified in the two developmental stages over 2 years,and were largely responsible for the segregation of resistance in the ILs. In 2007, the Lemont alleles at all loci in the seedling stage, except QBbr10 to C4, increased lesion length (LL) or decreased resistance. The Teqing allele at the Xa4 locus acted as a resistance gene against C2 and C4, but acted as a M-QTL when its resistance was overcome by the virulent race C5. MQTLs showed a degree of race specificity and had a cumulative effect on resistance. Most M-QTLs (94%) consistently expressed resistance to the same race at the seedling and adult stages, indicating that a high degree of genetic overlap exists between Xoo resistance at both developmental stages in rice. Among the digenic interactions, most co-introgressed Lemont alleles at the two epistatic loci lead to significantly smaller LL with all three races, compared to other types of interacting alleles at both development stages. The results indicate that a high level of resistance may be achieved by the cumulative effect of multiple M-QTLs, including the residual effects of “defeated” major resistance genes and the epistatic effects of co-introgression from diverse susceptible varieties.
Young Chul Kim,Hikaru Suzuki,Wen-Xie Xu,Hikaru Hashitani,Woong Choi,Hyo-Yung Yun,Seon-Mee Park,Sei Jin Youn,Sang-Jeon Lee,Sang Jin Lee 대한생리학회-대한약리학회 2008 The Korean Journal of Physiology & Pharmacology Vol.12 No.6
The properties of voltage dependent Ca<sup>2+</sup> current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c<i>-Kit</i> positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min<sup>−1</sup>, respectively, in the presence of 1</SUP>ՌM nifedipine. Single ICC-MY isolated by enzyme treatment also showed c<i>-Kit</i> immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K<sup>+</sup>-rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs<sup>+</sup>-rich pipette solution at <i>V</i><sub>h</sub>=−80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1ՌM) blocked VDIC. Therefore, we successfully isolated c<i>-Kit</i> positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca<sup>2+</sup> currents (VDCC<sub>L</sub>).
Xiaoqing Lin,Renjie Li,Qingshi Wen,Jinglan Wu,Jiansheng Fan,Xiaohong Jin,Wenbin Qian,Dong Liu,Xiaochun Chen,Yong Chen,Jingjing Xie,Jianxin Bai,Han-Jie Ying 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2
Removal of biobutanol from acetone-butanolethanol (ABE) fermentation broth can be achieved by fixed-bed sorption by means of KA-I resin, and the relevant breakthrough curves would provide much valuable information to help design a continuous fixed-bed sorption process in field application. In the present study, the effects of several important design parameters, i.e., initial butanol concentration (Cf: 3.0 ~ 30.0 g/L), inlet flow rate (Qf: 0.5~ 5.5 mL/min) and adsorbent bed height (Z: 4.2 ~ 18.0 cm),on the adsorption breakthrough curves of KA-I resin in a fixed-bed column were investigated. It was found that the amount of adsorbed butanol at breakthrough point was increased with an increase in the value of Cf and Z; and with decrease in the value of Qf. However, the maximum sorption capacities of butanol at saturated point were basically unchanged. Three well-established fixed-bed adsorption models, namely Thomas, Yoon-Nelson and Adams-Bohart,were applied to predict the breakthrough curves and to determine the characteristic parameters of fixed-bed column,which are the basis for the process design at a real scale. Good agreement between the theoretical breakthrough curves and the experimental result were observed using Thomas and Yoon-Nelson models.
Kim, Young-Chul,Suzuki, Hikaru,Xu, Wen-Xie,Hashitani, Hikaru,Choi, Woong,Yun, Hyo-Yung,Park, Seon-Mee,Youn, Sei-Jin,Lee, Sang-Jeon,Lee, Sang-Jin The Korean Society of Pharmacology 2008 The Korean Journal of Physiology & Pharmacology Vol.12 No.6
The properties of voltage dependent $Ca^{2+}$ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times $min^{-1}$ respectively, in the presence of $1{\mu}M$ nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under $K^+$ -rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With $Cs^+$-rich pipette solution at $V_h=-80\;mV$, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine ($1{\mu}M$) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent $Ca^{2+}$ currents ($VDCC_L$).
The Role of ATP-Sensitive K+ Channel (KATP channel) in Longitudinal Smooth Muscles of Murine Uterus
( Seung Hwa Hong ),( Kyu Sang Kyeong ),( Yeon Jin Park ),( Iii Woon Ji ),( Eun Hwan Jeong ),( Hak Soon Kim ),( Chan Hyung Kim ),( Woong Choi ),( Wen Xie Xu ),( Ra Young You ),( Sang Jin Lee ),( Young 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-
Effect of gelatin nano-coating containing Gardenia pigment on the preservation of pork slices
Yong Liu,Zi-Hao Liu,Chang-Qi Luo,Chun-Tao Xiao,Wen-Yu Zhou,Wen-Jin Xie 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.4
The nano-coating composed of gelatin and Gardenia pigment (GP) was successfully prepared and showed strong antioxidant activity. The average particle sizes of the nano-coating containing 0.1% and 0.3% GP were 269.58 and 394.13 nm, respectively. The pork slices uncoated and coated with the nano-coating were preserved at 4 C for 15 days. The pork slices’ pH, total volatile basic nitrogen (TVB-N), total viable counts (TVC), water-bind- ing capacity (WHC), and thiobarbituric acid reactive sub- stances (TBARS) were measured to assess the preservation effect of the nano-coating. The results showed that the pork coated with the nano-coating had lower pH, TVC, TVB-N, TBARS, and higher WHC, significantly different (p \ 0.05) than the uncoated pork. It is suggested that the proposed nano-coating can be used to effectively improve the pork’s quality and shelf life during refrigeration storage.