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      • Robust FeCo nanoparticles embedded in a N-doped porous carbon framework for high oxygen conversion catalytic activity in alkaline and acidic media

        Gao, Xuan-Wen,Yang, Junghoon,Song, Kyeongse,Luo, Wen-Bin,Dou, Shi-Xue,Kang, Yong-Mook The Royal Society of Chemistry 2018 Journal of Materials Chemistry A Vol.6 No.46

        <P>FeCo alloy nanoparticles were nucleated onto graphitic carbon layers through the pyrolysis of polydopamine (PDA) sub-micrometer spheres to form a highly active electrocatalytic system that exhibits excellent oxygen conversion catalytic activity in both alkaline and acidic media. Owing to the strong metal chelation capability during the chemical modification and high sp<SUP>2</SUP>-dominant carbon yield of PDA, an abundance of non-precious-metal ions were easily trapped and absorbed into the PDA segments at room temperature by catechol and amine functional groups, followed by the <I>in situ</I> nucleation of FeCo alloy nanoparticles on graphitic carbon layers during the pyrolysis. The contents of graphitic nitrogen and pyridinic nitrogen were significantly increased by the presence of the non-precious-metal ions during carbonization as well, which is a result of the chelation effect of non-precious-metal atoms. Meanwhile, the FeCo nanoparticles (diameter < 5 nm) were protected by the multi-layer-graphene-like carbon layer from the harsh acid and uniformly anchored on graphitic carbon sub-microspheres, which can greatly improve the catalytic durability, particularly in acidic media. From the perspective of the whole catalytic system being used as an air electrode in rechargeable Zn-air batteries, the porous nitrogen-doped graphitic carbon framework was functionalised as a continuous conductive framework due to its catalytic activity towards high oxygen conversion and good electrical conductivity.</P>

      • KCI등재

        CircZNF609 Aggravated Myocardial Ischemia Reperfusion Injury via Mediation of miR-214-3p/PTGS2 Axis

        Wen-Qiang Tang,Feng-Rui Yang,Ke-Min Chen,Huan Yang,Yu Liu,Bo Dou 대한심장학회 2022 Korean Circulation Journal Vol.52 No.9

        Background and Objectives: Circular RNAs were known to play vital role in myocardial ischemia reperfusion injury (MIRI), while the role of CircZNF609 in MIRI remains unclear. This study was aimed to investigate the function of CircZNF609 in MIRI. Methods: Hypoxia/reoxygenation (H/R) model was established to mimic MIRI in vitro. Quantitative polymerase chain reaction was performed to evaluate gene transcripts. Cellular localization of CircZNF609 and miR-214-3p were visualized by fluorescence in situ hybridization. Cell proliferation was determined by CCK-8. TUNEL assay and flow cytometry were applied to detect apoptosis. Lactate dehydrogenase was determined by commercial kit. ROS was detected by DCFH-DA probe. Direct interaction of indicated molecules was determined by RIP and dual luciferase assays. Western blot was used to quantify protein levels. In vivo model was established to further test the function of CircZNF609 in MIRI. Results: CircZNF609 was upregulated in H/R model. Inhibition of CircZNF609 alleviated H/R induced apoptosis, ROS generation, restored cell proliferation in cardiomyocytes and human umbilical vein endothelial cells. Mechanically, CircZNF609 directly sponged miR-214-3p to release PTGS2 expression. Functional rescue experiments showed that miR-214-3p/PTGS2 axis was involved in the function of circZNG609 in H/R model. Furthermore, data in mouse model revealed that knockdown of CircZNF609 significantly reduced the area of myocardial infarction and decreased myocardial cell apoptosis. Conclusions: CircZNF609 aggravated the progression of MIRI via targeting miR-214-3p/PTGS2 axis, which suggested CircZNF609 might act as a vital modulator in MIRI.

      • KCI등재

        3-Hydroxy-3-methyl glutaryl coenzyme A reductase is required for ovarian development in the oriental fruit fly Bactrocera dorsalis (Hendel)

        Wen-Jia Yang,Kang-Kang Xu,Wei Dou,Can Li,JinjunWang 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.3

        3-Hydroxy-3-methyl glutaryl coenzyme A reductase (HMGR) is an important rate-limiting enzyme in the mevalonate pathway, which is critical for the synthesis of juvenile hormone and insect development. In this study, a full-length cDNA encoding HMGR (BdHMGR) was cloned from the oriental fruit fly, Bactrocera dorsalis (Hendel). The open reading frame of BdHMGR encoded 928 amino acids and shared a high degree of identity with other known HMGRs. The expression levels of BdHMGR were high during the early and lately pupal stages, and were low at the larval and mid pupal stages. In the adult stages, the highest level of BdHMGR was detected on day-10 of the adult lifecycle, and the expression levels in females were greater than those in males. The highest tissuespecific expression was in the ovary, followed by fat body and other tissues. Injection of double-stranded RNA (dsRNA) of BdHMGR into adult females significantly reduced BdHMGR transcript levels, and inhibited ovarian development and affected the expression of two yolk protein genes. After dissection, we found that one or two of the spheroids of ovaries from the BdHMGR dsRNA-injected flies were amorphous and reduced in size as compared with the controls. The results suggest that BdHMGR is required for ovarian development in B. dorsalis and may be a potential target for pest control.

      • Effectively remediating spiramycin from production wastewater through hydrolyzing its functional groups using solid superacid TiO<sub>2</sub>/SO<sub>4</sub>

        Yang, Wen,Ok, Yong Sik,Dou, Xiaomin,Zhang, Yu,Yang, Min,Wei, Dongbin,Xu, Peng Elsevier 2019 Environmental research Vol.175 No.-

        <P><B>Abstract</B></P> <P>Breaking down the structural bonds and eliminating the functional groups are more efficient than destroying the whole molecule in antibiotic production wastewater (APW) pretreatment before further biotreatment. Two sulfated titania (TiO<SUB>2</SUB>/SO<SUB>4</SUB>) solid superacids, SSA1 and SSA2 were synthesized, characterized and used for hydrolytic pretreatment of spiramycin in APW. Spiramycin removal followed an order of SSA2>SSA1>TiO<SUB>2</SUB>≈pH = 3>control. The hydrolytic efficiencies increased at elevated temperature from 25 °C to 65 °C. The hydrolytic kinetics followed a first-order model and SSA2 performed the fastest. The performances were positively correlated with both the total acidity determined by <I>n</I>-butylamine titration and the strength of acid sites measured by NH<SUB>3</SUB>-temperature-programmed desorption (TPD). The residual solution for SSA2 presented the least antibacterial potency and anaerobic inhibition among all treatments. The hydrolyzed product was identified as the <I>m/z</I> 699.4321 fragment using UPLC-Q/TOF-MS, which was formed after losing a functional mycarose moiety from the parent molecular. The solid superacids were effective in selectively eliminating 433 mg/L of spiramycin and the antibacterial potencies of the spiramycin production wastewater, which contained very high concentrations of COD (33,000 mg/L). This hydrolytic method avoids using and handling hazardous and corrosive mineral acids on site. It is attractive as a selective catalytic pretreatment method to cleave antibiotics’ functional groups and to reduce its inhibitory effects before sequential biotreatments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Solid superacids were made by calcining the reaction products of H<SUB>2</SUB>SO<SUB>4</SUB> and titania. </LI> <LI> Functional group breakage was catalyzed by the acid sites of solid superacids. </LI> <LI> The Lewis and Brønsted acidic sites all contributed to the hydrolytic reaction. </LI> <LI> The antibacterial potency and the anaerobic inhibition were effectively decreased. </LI> <LI> Superacids performed well in hydrolytic pretreatment of the production wastewater. </LI> </UL> </P>

      • KCI등재

        Preparation of highly efficient self-healing anticorrosion epoxy coating by integration of benzotriazole corrosion inhibitor loaded 2D-COF

        Tengfei Liu,Wen Li,Chenyang Zhang,Wei Wang,Wenwen Dou,Shougang Chen 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

        In this work, multi-layer stacked two-dimensional (2D) covalent organic framework of TpPa-1 wassynthesized as nanocontainer to fabricate self-healing epoxy coating. The corrosion inhibitor ofbenzotriazole (BTA) was loaded into porous TpPa-1 (Tp: 1,3,5-Triformylphrogroglucinol, Pa-1: 1,4-phenylenediamine), and the load capacity was further improved by electrostatic adsorption. Then, 0.5 wt. % BTA/TpPa-1 and 0.5 wt.% TpPa-1 were added to the epoxy coating, respectively. The electrochemicalImpedance Spectroscopy (EIS) test shown that after 60 days of immersion, the impedance of the BTA/TpPa-1/EP in low-frequency region was 6.45108V cm2, while the impedance corresponding to theTpPa-1/EP coating was 9.04107V cm2. In addition, in the localized electrochemical impedancespectroscopy (LEIS) test, as the immersion time was extended, the impedance of TpPa-1/EP coating at thedefect continued to decrease, while the impedance at the defect of the BTA/TpPa-1/EP coating shown anupward trend, showing self-healing performance. This was due to the pH change when the metal at thedefect is corroded, which caused the BTA to be released from the nanocontainer and adhered to the metalsurface to form a protectivefilm. The improvement of the anti-corrosion performance of BTA/TpPa-1/EPcoating mainly comes from two aspects: (1) The good dispersion of TpPa-1 in EP coating improves thephysical shielding effect of the coating; (2) The controlled release of BTA can Realize the continuousformation of the bare metal surface protective layer.

      • KCI등재

        SERIES REPRESENTATIONS FOR SOME SPECIAL FUNCTIONS

        LI YIN,WEN-YAN CUI,XIANG-KAI DOU 장전수학회 2016 Proceedings of the Jangjeon mathematical society Vol.19 No.1

        In this paper, we show some series representations for the generalized complete elliptic integrals, generalized trigonometric functions and incomplete beta function.

      • KCI등재

        Experimental Study on Combined Column of Steel Pipe

        Liang Zheng,Shengrun Dou,Wen Wang,Ying Gao,Hongwei Gai 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.1

        To study the properties of various steel-pipe combined columns, ten steel pipe combined columns were designed, including fi ve axial compression specimens and fi ve eccentric compression specimens. The test mainly investigated the infl uence of the combination of a fl ange steel pipe and web steel pipe on the combined column, including the overall failure mode, local failure mode, load–displacement curve, bearing capacity, and stability of the fl ange steel pipe and web steel pipes. The test results showed that the overall and local failure modes of the axial compression specimens with diff erent steel pipe combinations were diff erent. The failure modes of the eccentric compression specimens with diff erent steel pipe combinations were almost identical. Except for the eccentric compression specimen PZH2, none of the other eccentric compression specimens exhibited local buckling. The combination of the fl ange steel pipes and web steel pipes near the fl ange steel pipes had an obviously eff ect on the bearing capacity, while the combination of steel pipes in the middle of the web had a lower eff ect on the bearing capacity. During the loading process, the fl ange and web steel pipe did not lose stability, and the weld between the steel pipes of the axial compression specimen and the eccentric compression specimen was not damaged. There was no obvious separation between the unwelded parts of the steel pipes.

      • SCOPUSKCI등재SCIE

        Effects of fireworks on air quality in the main urban area of Nanchong City during the spring festival of 2014-2019

        Yifan Qian,Xu Yuan,Wen Dou,Jian Hu,Jie Xia,Danyu Li,Qing Zheng,Ping Zhang,Qiumei Quan,Yunxiang Li 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.2

        The sources and variation trend of ambient air pollutants, the duration of pollution process and the conditions for decontamination were analyzed in this essay based on the ambient air pollutants data, PM<SUB>2.5</SUB> source analysis data and meteorological factors in Nanchong City during 2014 to 2019. The results indicated that the concentrated discharge of fireworks on New Year’s Eve was the main reason for the sharp deterioration of the ambient air quality. PM<SUB>2.5</SUB> and PM<SUB>10</SUB> concentrations increased significantly after fireworks were set off, and the peak value appeared at about 02:00 on the Lunar New Year’s Day, but the value of PM<SUB>2.5</SUB>/PM<SUB>10</SUB> did not change significantly. The concentration of SO₂ in gaseous pollutants was slightly affected by fireworks, while NO₂, CO and O₃ were not affected basically. Having been affected by fireworks, the concentrations and percentages of K⁺, Cl⁻ and Mg<SUP>2+</SUP> of the water-solubility ions in PM<SUB>2.5</SUB> significantly increased at the 0.01 level. Meanwhile, the concentrations and percentages of the metal elements, including Cu, Pb, Mg, Al, K and Ba, significantly increased at the 0.05 level. Restricted to the topography and meteorological factors, the duration of the pollution was prolonged.

      • KCI등재후보

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