Association of the Porcine Cluster of Differentiation 4 Gene with T Lymphocyte Subpopulations and Its Expression in Immune Tissues

Xu, Jingen,Liu, Yang,Fu, Weixuan,Wang, Jiying,Wang, Wenwen,Wang, Haifei,Liu, Jianfeng,Ding, Xiangdong,Zhang, Qin Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.4

Cluster of differentiation 4 (CD4) is mainly expressed on $CD4^+$ T cells, which plays an important role in immune response. The aim of this study was to detect the association between polymorphisms of the CD4 gene and T lymphocyte subpopulations in pigs, and to investigate the effects of genetic variation on the CD4 gene expression level in immune tissues. Five missense mutations in the CD4 gene were identified using DNA pooling sequencing assays, and two main haplotypes (CCTCC and AGCTG) in strong linkage disequilibrium (with frequencies of 50.26% and 46.34%, respectively) were detected in the population of Large White pigs. Our results indicated that the five SNPs and the two haplotypes were significantly associated with the proportions of $CD4^-CD8^-$, $CD4^+CD8^+$, $CD4^+CD8^-$, $CD4^+$ and $CD4^+/CD8^+$ in peripheral blood (p<0.05). Gene expression analysis showed the mRNA level of the CD4 gene in thymus was significantly higher than that in lymph node and spleen (p<0.05). However, no significant difference was observed between animals with CCTCC/CCTCC genotype and animals with AGCTG/AGCTG genotype in the three immune tissues (p>0.05). These results indicate that the CD4 gene may influence T lymphocyte subpopulations and can be considered as a candidate gene affecting immunity in pigs.

Genome-wide locus-specific DNA methylation repatterning may facilitate rapid evolution of mercury resistance in rice

Cong Weixuan,Li Ning,Wang Jinbin,Kang Ying,Miao Yiling,Xu Chunming,Wang Ziqi,Liu Tongtong,Gong Lei,Liu Bao,Ou Xiufang 한국유전학회 2022 Genes & Genomics Vol.44 No.3

Background: Albeit a relatively stable epigenetic modification, DNA methylation in plants can be repatterned and play important roles in response to biotic and abiotic stresses. However, whether DNA methylation dynamics may contribute to cope with mercury (Hg) stress in plants remains to be fully investigated. Objective: To probe the potential roles of DNA methylation dynamics in coping with Hg stress in rice. Methods: Whole-genome bisulfite sequencing was used to profile the DNA methylation patterns of a rice Hg-resistant line (RHg) selected from a heterozygous mutant of the DNA methyltransferase 1 gene (OsMET1+/-), together with its Hg-sensitive wild-type plants of cv. Nipponbare (Nip) under both normal and Hg stress conditions. Results: Genome-wide locus-specific differential methylation regions (DMRs) were detected between RHg and Nip under normal condition, the predominant DMR patterns were CG hypo-DMRs, CHG hypo-DMRs and CHH hyper-DMRs. In both lines, more hyper- than hypo-DMRs were detected at all three sequence contexts (CG, CHG and CHH) under Hg stress relative to normal condition. Comparison of DNA methylation changes in the two lines under Hg stress indicates that RHg had a more dynamic methylome than the control (Nip). Original DMRs in RHg trended to transform to opposite status (from hyper- to hypo- or vice versa) under Hg stress condition. Gene ontology analysis revealed that Hg-resistance-related DMGs were enriched in diverse biological processes. Conclusions: Our results suggest genome-wide locus-specific DNA methylation repatterning can facilitate rapid acquisition of Hg resistance in rice.

Tissues Expression, Polymorphisms of IFN Regulatory Factor 6 (IRF6) Gene and Their Associated with Immune Traits in Three Pig Populations

Liu, Yang,Xu, Jingeng,Fu, Weixuan,Weng, Ziqing,Niu, Xiaoyan,Liu, Jianfeng,Ding, Xiangdong,Zhang, Qin Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.2

Interferon regulatory factor 6 (IRF6) gene is a member of the IRF-family, and plays functionally diverse roles in the regulation of the immune system. In this report, the 13,720 bp porcine IRF6 genomic DNA structure was firstly identified with a putative IRF6 protein of 467 amino acids. Alignment and phylogenetic analysis of the porcine IRF6 amino acid sequences with their homologies to other species showed high identity (over 96%). Tissues expression of IRF6 mRNA was observed by RT-PCR, the results revealed IRF6 expressed widely in eight tissues. One SNP (HQ026023:1383 G>C) in exon7 and two SNPs (HQ026023:130 G>A; 232 C>T) in the 5′ promoter region of porcine IRF6 gene were demonstrated by DNA sequencing analysis. A further analysis of SNP genotypes associated with immune traits including IFN-${\gamma}$ and IL10 concentrations in serum was carried out in three pig populations including Large White, Landraces and Songliao Black pig (a Chinese indigenous breed). The results showed that the SNP (HQ026023:1383 G>C) was significantly associated with the level of IFN-${\gamma}$ (d 20) in serum (p = 0.038) and the ratio of IFN-${\gamma}$ to IL10 (d 20) in serum (p = 0.041); The other two SNPs (HQ026023:130 G>A; 232 C>T) were highly significantly associated with IL10 level in serum both at the day 20 (p = 0.005; p = 0.001) and the day 35 (p = 0.004; p = 0.006). Identification of the porcine IRF6 gene will help our further understanding of the molecular basis of the IFN regulation pathway in the porcine immune response. All these results should indicate that the IRF6 gene can be regarded as a molecular marker associated with the IL10 level in serum and used for genetic selection in the pig breeding.

Study on the Effect of Tween80 on the Properties of PVA/SCFs Composites

Liulan Lin,Weixuan Xu,Guomin Liu 한국고분자학회 2023 폴리머 Vol.47 No.5

Tissues Expression, Polymorphisms Identification of FcRn Gene and Its Relationship with Serum Classical Swine Fever Virus Antibody Level in Pigs

Liu, Yang,Wang, Chonglong,Liu, Zhengzhu,Xu, Jingen,Fu, Weixuan,Wang, Wenwen,Ding, Xiangdong,Liu, Jianfeng,Zhang, Qin Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.8

Neonatal Fc receptor (FcRn) gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G (IgG) and is responsible for IgG transport and stabilization. In this report, the 8,900 bp porcine FcRn genomic DNA structure was identified and putative FcRn protein included 356 amino acids. Alignment and phylogenetic analysis of the porcine FcRn amino acid sequences with their homologies of other species showed high identity. Tissues expression of FcRn mRNA was detected by real time quantitative polymerase chain reaction (Q-PCR), the results revealed FcRn expressed widely in ten analyzed tissues. One single nucleotide polymorphism (SNP) (HQ026019:g.8526 C>T) in exon6 region of porcine FcRn gene was demonstrated by DNA sequencing analysis. A further analysis of SNP genotypes associated with serum Classical Swine Fever Virus antibody (anti-CSFV) concentration was performed in three pig populations including Large White, Landrace and Songliao Black pig (a Chinese indigenous breed). Our results of statistical analysis showed that the SNP had a highly significant association with the level of anti-CSFV antibody (At d 20; At d 35) in serum (p = 0.008; p = 0.0001). Investigation of expression and polymorphisms of the porcine FcRn gene will help us in further understanding the molecular basis of the antibody regulation pathway in the porcine immune response. All these results indicate that FcRn gene might be regarded as a molecular marker for genetic selection of anti-CSFV antibody level in pig disease resistance breeding programmes.

A Conflict Measure Model for an Application to Green Supplier Selection

Mingrong Deng,Jin-Xiao Chen,Weixuan Xu 중앙대학교 한국전자무역연구소 2010 전자무역연구 Vol.8 No.4

As environmental issues are of increased concern, many enterprises pay close attention to green supply chain. The conflict among supply chain members should be evaluated since the long strategic relation is essential to cooperate and collaborate smoothly. Based on their attitudes toward important issues, a conflict evaluation model is proposed. Due to the particularity in aggregating conflicts, both the issue importance and aggregation structure are considered. The weight parameters in model are delivered from experience. The model is very effective in supplier selection under e-trade mode because the attitudes can be collected easily through information platform. This paper address the problem how to measure the conflict between two organizations based on their real attitudes toward some important issues, for example, environmental ones. 환경 문제가 높은 관심을 받게 되었기 때문에 많은 기업들은 녹색공급사슬에 면밀한 관심을 가지게 되었다. 장기적인 전략적 관계는 원활하게 협동하고 협력하기 위해서 필수적인 것이기 때문에 공급사슬 구성원들 사이의 갈등은 측정되어야만 한다. 중요한 문제에 대한 그들의 행동에 기초하여 갈등 측정 모델이 제시되었다. 갈등을 집합시키는데 있어서의 특수성 때문에 문제에 대한 중요성과 집합 구조가 고려된다. 모델 내에서의 가중치 매개변수는 경험으로부터 얻을 수 있다. 행동은 정보 플랫폼을 통하여 쉽게 수집될 수 있기 때문에 모델은 전자무역 방식 하에서 공급자의 선택에서 매우 효과적이다. 본 논문은 어떠한 중요한 이슈들, 예를 들면 환경이슈들에 대한 기업들의 실질적인 태도에 근거하여 2개의 기업 간의 갈등을 어떻게 측정하는가의 문제를 다룬다.