Polymorphism, Expression of Natural Resistance-associated Macrophage Protein 1 Encoding Gene (NRAMP1) and Its Association with Immune Traits in Pigs

Ding, Xiaoling,Zhang, Xiaodong,Yang, Yong,Ding, Yueyun,Xue, Weiwei,Meng, Yun,Zhu, Weihua,Yin, Zongjun Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.8

Natural resistance-associated macrophage protein 1 encoding gene (NRAMP1) plays an important role in immune response against intracellular pathogens. To evaluate the effects of NRAMP1 gene on immune capacity in pigs, tissue expression of NRAMP1 mRNA was observed by real time quantitative polymerase chain reaction (PCR), and the results revealed NRAMP1 expressed widely in nine tissues. One single nucleotide polymorphism (SNP) (ENSSSCG00000025058: g.130 C>T) in exon1 and one SNP (ENSSSCG00000025058: g.657 A>G) in intron1 region of porcine NRAMP1 gene were demonstrated by DNA sequencing and PCR-RFLP analysis. A further analysis of SNP genotypes associated with immune traits including contain of white blood cell (WBC), granulocyte, lymphocyte, monocyte (MO), rate of cytotoxin in monocyte (MC) and $CD4^-CD8^+$ T lymphocyte subpopulations in blood was carried out in four pig populations including Large White and three Chinese indigenous breeds (Wannan Black, Huai pig and Wei pig). The results showed that the SNP (ENSSSCG00000025058: g.130 C>T) was significantly associated with level of WBC % (p = 0.031), MO% (p = 0.024), MC% (p = 0.013) and $CD4^-CD8^+$ T lymphocyte (p = 0.023). The other SNP (ENSSSCG00000025058: g.657 A>G) was significantly associated with the level of MO% (p = 0.012), MC% (p = 0.019) and $CD4^-CD8^+$ T lymphocyte (p = 0.037). These results indicate that the NRAMP1 gene can be regarded as a molecular marker for genetic selection of disease susceptibility in pig breeding.

Experimental and modeling study of viscoelastic behaviors of magneto-rheological shear thickening fluids

Peng, Gang Rou,Li, Weihua,Tian, Tong Fei,Ding, Jie,Nakano, Masami 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.2

Nowadays, both Magneto-rheological Fluid (MRF) and Shear Thickening Fluid (STF) have separately attracted considerable interest due to the fast reversible response to either external magnetic field or abrupt shearing loading. In this paper, we fabricated a combined phase of Magneto-rheological Shear Thickening Fluid (MRSTF), where the 25 wt% STF is applied as medium phase with the addition of varied fractions of iron particle. The investigation of the dynamic behavior of this novel material under oscillatory shear was launched in a parallel-plate rheometer. The relevance of the dynamic behavior to strain amplitude, frequency and external magnetic field were investigated and discussed. A four-parameter viscoelastic model was applied to reconstruct the mechanical behavior of the MRSTF under different working conditions, and the parameters were identified within the Matlab optimization algorithm. The comparison between the experimental data and the model prediction results indicated that the four-parameter model could predict viscoelastic material with desired accuracy. The MRSTF exhibits features of both components, while prone more to MRF with the inception of external field excitations.

Experimental and modeling study of viscoelastic behaviors of magneto-rheological shear thickening fluids

Gang Rou Peng,Weihua Li,Tongfei Tian,Jie Ding,Masami Nakano 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.2

Nowadays, both Magneto-rheological Fluid (MRF) and Shear Thickening Fluid (STF) have separatelyattracted considerable interest due to the fast reversible response to either external magnetic field or abruptshearing loading. In this paper, we fabricated a combined phase of Magneto-rheological Shear ThickeningFluid (MRSTF), where the 25 wt% STF is applied as medium phase with the addition of varied fractionsof iron particle. The investigation of the dynamic behavior of this novel material under oscillatory shear waslaunched in a parallel-plate rheometer. The relevance of the dynamic behavior to strain amplitude,frequency and external magnetic field were investigated and discussed. A four-parameter viscoelastic modelwas applied to reconstruct the mechanical behavior of the MRSTF under different working conditions, andthe parameters were identified within the Matlab optimization algorithm. The comparison between theexperimental data and the model prediction results indicated that the four-parameter model could predictviscoelastic material with desired accuracy. The MRSTF exhibits features of both components, while pronemore to MRF with the inception of external field excitations.

Joint Space-time Coding and Power Domain Non-orthogonal Multiple Access for Future Wireless System

( Jin Xu ),( Hanqing Ding ),( Zeqi Yu ),( Zhe Zhang ),( Weihua Liu ),( Xueyan Chen ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.1

According to information theory, non-orthogonal transmission can achieve the multiple-user channel capacity with an onion-peeling like successive interference cancellation (SIC) based detection followed by a capacity approaching channel code. However, in multiple antenna system, due to the unideal characteristic of the SIC detector, the residual interference propagated to the next detection stage will significantly degrade the detection performance of spatial data layers. To overcome this problem, we proposed a modified power-domain non-orthogonal multiple access (P-NOMA) scheme joint designed with space-time coding for multiple input multiple output (MIMO) NOMA system. First, with proper power allocation for each user, inter-user signals can be separated from each other for NOMA detection. Second, a well-designed quasi-orthogonal space-time block code (QO-STBC) was employed to facilitate the SIC-based MIMO detection of spatial data layers within each user. Last, we proposed an optimization algorithm to assign channel coding rates to balance the bit error rate (BER) performance of those spatial data layers for each user. Link-level performance simulation results demonstrate that the proposed time-space-power domain joint transmission scheme performs better than the traditional P-NOMA scheme. Furthermore, the proposed algorithm is of low complexity and easy to implement.

Experimental and modelling study of the effect of temperature on shear thickening fluids

Tongfei Tian,Gang Rou Peng,Weihua Li,Jie Ding,Masami Nakano 한국유변학회 2015 Korea-Australia rheology journal Vol.27 No.1

This paper is a study of the effect that temperature has on a shear thickening fluid made from ethylene glycol and fumed silica, with 20%-26% weight fractions. Three typical temperatures, ranging from 20oC to 60oC, were selected to study the shear rate dependence of viscosity. The temperature tests showed that a high temperature increased the critical shear rate and lowered the shear thickening ratio. A viscosity function was proposed to represent the three characteristic regions in typical shear thickening fluid and to predict viscosity at different temperatures

Solid-phase synthesis of perovskite using spent SCR catalyst and calcium carbonate and its application as ceramic opacifier

Han Zhang,Jianmeng Zhang,Hao Ding,Yangzi Li,Sijia Sun,Weihua Ao,Yu Liang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

In this study, the synthetic perovskite powder (SPP) was produced by solid-phase method using S-SCRand calcium carbonate (CaCO3), and its structure, morphology, and application as ceramic opacifier werestudied. The results show that SPP with a single perovskite phase can be obtained by grinding and calciningraw materials. Adding SPP as opacifier of sanitary ceramics for firing, a white ceramic glaze withexcellent opacity was obtained. Among the glaze chromaticity values, L* is 93.09, a* is 0.76 and b* is4.98. The opacity of the glaze is stronger than that of the glaze using ZrSiO4 and TiO2 as opacifiers, andthe whiteness of the glaze is significantly greater than that of glaze with TiO2. The ceramic glaze layerusing SPP is mainly composed of glass phase, titanite and quartz, of which titanite is the opacified phase. Importantly, TiO2 is pre-combined into perovskite to make it unable to freely and avoid its own hightemperaturephase transition reaction, which is the mechanism to inhibit the yellowing of glaze surfacecaused by direct use of TiO2. This study can play a positive role in improving the utilization value of S-SCRand eliminating the yellowing of ceramic glazes using TiO2 as opacifier.

Unified Overload Method of Slope Stability Analysis Based on Potential Sliding Direction

Haibin Xue,Faning Dang,Xiaotao Yin,Weihua Ding 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.9

The overload method is difficult to be promoted in slope stability analysis for its disunity of loading forms and directions. Based on the traditional overload method and the Strength Reduction Method (SRM) in which the limit equilibrium state of the slope was reached by reducing sliding resisting force without changing the sliding force, a new way to reach the limit equilibrium state of the slope was developed by increasing sliding force without changing resisting force. Referring the loading forms in Gravity Increase Method (GIM) and sliding direction determination in Vector Sum Method (VSM), the theoretical relationship was built between overload coefficient and safety factor of Vector Sum Method (VSM) and the unified overload method based on overall potential sliding direction was proposed. The loading forms and directions were unified by this method, respectively. Sliding surface could be determined while solving the safety factor and the developing direction of overload method in slope stability analysis application was indicated. Three representative slopes with fixed sliding surfaces and two slopes with unknown sliding surfaces were taken as examples to compare results from Limit Equilibrium Method (LEM), Strength Reduction Method (SRM), Vector Sum Method (VSM), Gravity Increase Method (GIM) and overloading method along the horizontal direction with each other. The safety factor resulted from the method proposed in this paper was close to the one from Vector Sum Method (VSM) and the location of sliding surface was close to the one from Strength Reduction Method (SRM). Thus the reliability of the method was testified.

MiR-26a promotes apoptosis of porcine granulosa cells by targeting the 3β-hydroxysteroid-Δ24-reductase gene

Zhang, Xiaodong,Tao, Qiangqiang,Shang, Jinnan,Xu, Yiliang,Zhang, Liang,Ma, Yingchun,Zhu, Weihua,Yang, Min,Ding, Yueyun,Yin, Zongjun Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.4

Objective: Apoptosis of ovarian granulosa cells (GCs) affects mammalian follicular development and fecundity. This study aimed to explore the regulatory relationship between microRNA-26a (miR-26a) and the 3β-hydroxysteroid-Δ24-reductase gene (DHCR24) gene in porcine follicular granular cells (pGCs), and to provide empirical data for the development of methods to improve the reproductive capacity of pigs. Methods: The pGCs were transfected with miR-26a mimic, miR-26a inhibitor and DHCR24-siRNA in vitro. The cell apoptosis rate of pGCs was detected by the flow cytometry. The secretion levels of estradiol (E2) and progesterone (P) in pGCs were detected by enzyme-linked immunosorbent assay. Double luciferase validation system was used to detect the binding sites between miR-26a and DHCR24 3'-UTR region. Qualitative real-time polymerase chain reaction and Western blotting were used to verify the DHCR24 mRNA and protein expression in pGCs, respectively, after transfecting with miR-26a mimic and miR-26a inhibitor. Results: Results showed that enhancement of miR-26a promoted apoptosis, and inhibited E2 and P secretion in pGCs. Meanwhile, inhibition of DHCR24 also upregulated the Caspase-3 expression, reduced the BCL-2 expression, promoted pGCs apoptosis, and inhibited E2 and P secretion in pGCs. There were the binding sites of miR-26a located within DHCR24 3'-UTR. Up-regulation of miR-26a inhibited DHCR24 mRNA and protein expression in pGCs. Conclusion: This study demonstrates that miR-26a can promote cell apoptosis and inhibit E2 and P secretion by inhibiting the expression of DHCR24 in pGCs.