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RISS 인기검색어
- 검색키워드
- 저자 : Wanqi Liang (검색결과 5 건)
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Protective Immune Response of Bacterially-Derived Recombinant FaeG in Piglets
Huang Yahong,Wanqi Liang,Aihu Pan,Zhiai Zhou,Qiang Wang,Cheng Huang,Jianxiu Chen,Dabing Zhang 한국미생물학회 2006 The journal of microbiology Vol.44 No.5
FaeG is the key factor in the infection process of K88ad enterotoxigenic Escherichia coli(ETEC) fimbrial adhesin. In an attempt to determine the possibility of expressing recombinant FaeG with immunogenicity for a new safe and high-production vaccine in E. coli, we constructed the recombinant strain, BL21 (DE3+K88), which harbors an expression vector with a DNA fragment of faeG, without a signal peptide. Results of 15% SDS-polyacrylamide slab gel analysis showed that FaeG can be stably over-expressed in BL21 (DE3+K88) as inclusion bodies without FaeE. Immunoglobulin G (IgG) and M (IgM) responses in pregnant pigs, with boost injections of the purified recombinant FaeG, were detected 4 weeks later in the sera and colostrum. An in vitro villius-adhesion assay verified that the elicited antibodies in the sera of vaccinated pigs were capable of preventing the adhesion of K88ad ETEC to porcine intestinal receptors. The protective effect on the mortality rates of suckling piglets born to vaccinated mothers was also observed one week after oral challenge with the virulent ETEC strain, C83907 (K88ad, CT+, ST+). The results of this study proved that the adhesin of proteinaceous bacterial fimbriae or pili could be overexpressed in engineered E. coli strains, with protective immune responses to the pathogen.
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Protective Immune Response of Bacterially-Derived Recombinant FaeG in Piglets
Yahong, Huang,Liang, Wanqi,Pan, Aihu,Zhou, Zhiai,Wang, Qiang,Huang, Cheng,Chen, Jianxiu,Zhang, Dabing The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.5
FaeG is the key factor in the infection process of K88ad enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin. In an attempt to determine the possibility of expressing recombinant FaeG with immunogenicity for a new safe and high-production vaccine in E. coli, we constructed the recombinant strain, BL21 (DE3+K88), which harbors an expression vector with a DNA fragment of faeG, without a signal peptide. Results of 15% SDS-polyacrylamide slab gel analysis showed that FaeG can be stably over-expressed in BL21 (DE3+K88) as inclusion bodies without FaeE. Immunoglobulin G (IgG) and M (IgM) responses in pregnant pigs, with boost injections of the purified recombinant FaeG, were detected 4 weeks later in the sera and colostrum. An in vitro villius-adhesion assay verified that the elicited antibodies in the sera of vaccinated pigs were capable of preventing the adhesion of K88ad ETEC to porcine intestinal receptors. The protective effect on the mortality rates of suckling piglets born to vaccinated mothers was also observed one week after oral challenge with the virulent ETEC strain, $C_{83907}$ (K88ad, $CT^+,\;ST^+$). The results of this study proved that the adhesin of proteinaceous bacterial fimbriae or pili could be overexpressed in engineered E. coli strains, with protective immune responses to the pathogen.
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Yi, Jakyung,Moon, Sunok,Lee, Yang-Seok,Zhu, Lu,Liang, Wanqi,Zhang, Dabing,Jung, Ki-Hong,An, Gynheung American Society of Plant Biologists 2016 Plant Physiology Vol.170 No.3
<P>After meiosis, tapetal cells in the innermost anther wall layer undergo program cell death (PCD)-triggered degradation. This step is essential for microspore development and pollen wall maturation. We identified a key gene, Defective Tapetum Cell Death 1 (DTC1), that controls this degeneration by modulating the dynamics of reactive oxygen species (ROS) during rice male reproduction. Mutants defective in DTC1 exhibit phenotypes of an enlarged tapetum and middle layer with delayed degeneration, causing male sterility. The gene is preferentially expressed in the tapetal cells during early anther development. In dtc1 anthers, expression of genes encoding secretory proteases or lipid transporters is significantly reduced, while transcripts of PCD regulatory genes, e.g. UDT1, TDR1, and EAT1/DTD, are not altered. Moreover, levels of DTC1 transcripts are diminished in udt1, tdr, and eat1 anthers. These results suggest that DTC1 functions downstream of those transcription factor genes and upstream of the genes encoding secretory proteins. DTC1 protein interacts with OsMT2b, a ROS scavenger. Whereas wild-type plants accumulate large amounts of ROS in their anthers at Stage 9 of development, those levels remain low during all stages of development in dtc1 anthers. These findings indicate that DTC1 is a key regulator for tapetum PCD by inhibiting ROS-scavenging activity.</P>
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Lu Zhu,Jianxin Shi,Guochao Zhao,Dabing Zhang,Wanqi Liang 한국식물학회 2013 Journal of Plant Biology Vol.56 No.1
The tapetum of the anther locule encloses the malereproductive cells and plays a supportive role for normalpollen development. However, the underlying mechanismremains less understood. Previously, we identified a completerecessive male sterile mutant, post-meiotic deficient anther1(pda1), with abnormal postmeiotic tapetal development. Inthis study we comprehensively characterized pda1. Chemicalanalysis uncovered that pda1 anther had significant lowerlevels of cutin monomers and cuticular waxes. PDA1 geneencodes an ATP-binding cassette (ABC) half-transporter,namely OsABCG15, which is conserved from algae tohigher plants. In situ RNA hybridization assay showed thatPDA1 is strongly expressed in tapetal cells, and weakly inmicrospores during the anther development. Additionally,the expression of two pollen exine biosynthetic genesCYP704B2 and CYP703A3 was dramatically reduced inpda1 mutant anthers. Altogether, these observations suggestthat the tapetum-expressed ABC transporter PDA1 plays acrucial role in secreting lipidic precursors from the tapetumto developing microspores and the anther epidermis.
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Chromatin interacting factor Os VIL 2 increases biomass and rice grain yield
Yang, Jungil,Cho, Lae‐,Hyeon,Yoon, Jinmi,Yoon, Hyeryung,Wai, Antt Htet,Hong, Woo‐,Jong,Han, Muho,Sakakibara, Hitoshi,Liang, Wanqi,Jung, Ki‐,Hong,Jeon, Jong‐,Seong,Koh, Hee John Wiley and Sons Inc. 2019 Plant biotechnology journal Vol.17 No.1
<P><B>Summary</B></P><P>Grain number is an important agronomic trait. We investigated the roles of chromatin interacting factor <I>Oryza sativa </I>VIN3‐LIKE 2 (OsVIL2), which controls plant biomass and yield in rice. Mutations in <I>OsVIL2</I> led to shorter plants and fewer grains whereas its overexpression (OX) enhanced biomass production and grain numbers when compared with the wild type. RNA‐sequencing analyses revealed that 1958 genes were up‐regulated and 2096 genes were down‐regulated in the region of active division within the first internodes of OX plants. Chromatin immunoprecipitation analysis showed that, among the downregulated genes, OsVIL2 was directly associated with chromatins in the promoter region of <I>CYTOKININ OXIDASE/DEHYDROGENASE2</I> (<I>OsCKX2</I>), a gene responsible for cytokinin degradation. Likewise, active cytokinin levels were increased in the OX plants. We conclude that OsVIL2 improves the production of biomass and grain by suppressing <I>OsCKX2</I> chromatin.</P>
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