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RISS 인기검색어
- 검색키워드
- 저자 : Jianxiu Chen (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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The Relaxant Effect of Propofol on Isolated Rat Intrapulmonary Arteries
Guangyan Zhang,Jianxiu Cui,Yijing Chen,Jue Ma 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.5
Propofol is a widely used anesthetic. Many studies have shown that propofol has direct effects on bloodvessels, but the precise mechanism is not fully understood. Secondary intrapulmonary artery ringsfrom male rats were prepared and mounted in a Multi Myograph System. The following constrictorswere used to induce contractions in isolated artery rings: high K+ solution (60 mmol/L); U46619 solution(100 nmol/L); 5-hydroxytryptamine (5-HT; 3 μmol/L); or phenylephrine (Phe; 1 μmol/L). The relaxationeffects of propofol were tested on high K+ or U46619 precontracted rings. Propofol also was addedto induce relaxation of rings preconstricted by U46619 after pretreatment with the nitric oxide synthaseinhibitor NG-nitro-L-arginine methyl ester (L-NAME). The effects of propofol on Ca2+ influx via theL-type Ca2+ channels were evaluated by examining contraction-dependent responses to CaCl2 in theabsence or presence of propofol (10 to 300 μmol/L). High K+ solution and U46619 induced remarkablecontractions of the rings, whereas contractions induced by 5-HT and Phe were weak. Propofol induceddose-dependent relaxation of artery rings precontracted by the high K+ solution. Propofol also inducedrelaxation of rings precontracted by U46619 in an endothelium-independent way. Propofol at differentconcentrations significantly inhibited the Ca2+-induced contractions of pulmonary rings exposed to highK+-containing and Ca2+-free solution in a dose-dependent manner. Propofol relaxed vessels precontractedby the high K+ solution and U46619 in an endothelium-independent way. The mechanism forthis effect may involve inhibition of calcium influx through voltage-operated calcium channels (VOCCs)and receptor-operated calcium channels (ROCCs).
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The Relaxant Effect of Propofol on Isolated Rat Intrapulmonary Arteries
Zhang, Guangyan,Cui, Jianxiu,Chen, Yijing,Ma, Jue The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.5
Propofol is a widely used anesthetic. Many studies have shown that propofol has direct effects on blood vessels, but the precise mechanism is not fully understood. Secondary intrapulmonary artery rings from male rats were prepared and mounted in a Multi Myograph System. The following constrictors were used to induce contractions in isolated artery rings: high $K^+$ solution (60 mmol/L); U46619 solution (100 nmol/L); 5-hydroxytryptamine (5-HT; $3{\mu}mol/L$); or phenylephrine (Phe; $1{\mu}mol/L$). The relaxation effects of propofol were tested on high $K^+$ or U46619 precontracted rings. Propofol also was added to induce relaxation of rings preconstricted by U46619 after pretreatment with the nitric oxide synthase inhibitor $N^G$-nitro-L-arginine methyl ester (L-NAME). The effects of propofol on $Ca^{2+}$ influx via the L-type $Ca^{2+}$ channels were evaluated by examining contraction-dependent responses to $CaCl_2$ in the absence or presence of propofol (10 to $300{\mu}mol/L$). High $K^+$ solution and U46619 induced remarkable contractions of the rings, whereas contractions induced by 5-HT and Phe were weak. Propofol induced dose-dependent relaxation of artery rings precontracted by the high $K^+$ solution. Propofol also induced relaxation of rings precontracted by U46619 in an endothelium-independent way. Propofol at different concentrations significantly inhibited the $Ca^{2+}$-induced contractions of pulmonary rings exposed to high $K^+$-containing and $Ca^{2+}$-free solution in a dose-dependent manner. Propofol relaxed vessels precontracted by the high $K^+$ solution and U46619 in an endothelium-independent way. The mechanism for this effect may involve inhibition of calcium influx through voltage-operated calcium channels (VOCCs) and receptor-operated calcium channels (ROCCs).
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Protective Immune Response of Bacterially-Derived Recombinant FaeG in Piglets
Huang Yahong,Wanqi Liang,Aihu Pan,Zhiai Zhou,Qiang Wang,Cheng Huang,Jianxiu Chen,Dabing Zhang 한국미생물학회 2006 The journal of microbiology Vol.44 No.5
FaeG is the key factor in the infection process of K88ad enterotoxigenic Escherichia coli(ETEC) fimbrial adhesin. In an attempt to determine the possibility of expressing recombinant FaeG with immunogenicity for a new safe and high-production vaccine in E. coli, we constructed the recombinant strain, BL21 (DE3+K88), which harbors an expression vector with a DNA fragment of faeG, without a signal peptide. Results of 15% SDS-polyacrylamide slab gel analysis showed that FaeG can be stably over-expressed in BL21 (DE3+K88) as inclusion bodies without FaeE. Immunoglobulin G (IgG) and M (IgM) responses in pregnant pigs, with boost injections of the purified recombinant FaeG, were detected 4 weeks later in the sera and colostrum. An in vitro villius-adhesion assay verified that the elicited antibodies in the sera of vaccinated pigs were capable of preventing the adhesion of K88ad ETEC to porcine intestinal receptors. The protective effect on the mortality rates of suckling piglets born to vaccinated mothers was also observed one week after oral challenge with the virulent ETEC strain, C83907 (K88ad, CT+, ST+). The results of this study proved that the adhesin of proteinaceous bacterial fimbriae or pili could be overexpressed in engineered E. coli strains, with protective immune responses to the pathogen.
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Protective Immune Response of Bacterially-Derived Recombinant FaeG in Piglets
Yahong, Huang,Liang, Wanqi,Pan, Aihu,Zhou, Zhiai,Wang, Qiang,Huang, Cheng,Chen, Jianxiu,Zhang, Dabing The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.5
FaeG is the key factor in the infection process of K88ad enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin. In an attempt to determine the possibility of expressing recombinant FaeG with immunogenicity for a new safe and high-production vaccine in E. coli, we constructed the recombinant strain, BL21 (DE3+K88), which harbors an expression vector with a DNA fragment of faeG, without a signal peptide. Results of 15% SDS-polyacrylamide slab gel analysis showed that FaeG can be stably over-expressed in BL21 (DE3+K88) as inclusion bodies without FaeE. Immunoglobulin G (IgG) and M (IgM) responses in pregnant pigs, with boost injections of the purified recombinant FaeG, were detected 4 weeks later in the sera and colostrum. An in vitro villius-adhesion assay verified that the elicited antibodies in the sera of vaccinated pigs were capable of preventing the adhesion of K88ad ETEC to porcine intestinal receptors. The protective effect on the mortality rates of suckling piglets born to vaccinated mothers was also observed one week after oral challenge with the virulent ETEC strain, $C_{83907}$ (K88ad, $CT^+,\;ST^+$). The results of this study proved that the adhesin of proteinaceous bacterial fimbriae or pili could be overexpressed in engineered E. coli strains, with protective immune responses to the pathogen.
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